Jay, Michael's post is correct and very well stated. In the US we estimate between 80 -85% of labs now use and RGA. The most common blend in the U.S is 95-5 which is 95% ethanol denatured with 5% methanol. Any company you purchase from should be happy to provide a C of A (certificate of analysis) to verify how they denature.
Hope this helps. Robert Jacox Product Manager Thermo Fisher Scientific Sent from my iPad > On May 12, 2018, at 5:21 PM, Michael Gudo (Morphisto GmbH) via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > CAUTION: This email originated from outside of the organization. Do not click > links or open attachments unless you recognize the sender and know the > content is safe. > > > Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96 > and 99 percent as the last two steps in tissue processing. These ethanols are > are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl > is not allowed as denaturation agent in Europe, otherwise you have to pay the > full alcohol tax. > > In our lab we we use 96 % ethanol and as last step and then, before going to > xylene we use isopropanol which has a better dehydration effect than ethanol. > 99 % ethanol takes water from the air and quite fast becomes a 96 or 95 % > ethanol and you will have some water left in the specimens when you to to > xylene. > So our experience are that the best quality of dehydration can be reached > with ethanol as high as possible and finally isopropanol before the xylene (2 > steps). > > With best regards > Michael > > >> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet >> <histonet@lists.utsouthwestern.edu>: >> >> Most places use "reagent alcohol" which, from most suppliers, is a mongrel >> blend of whatever is cheapest, and not even listed on the label. It's >> usually made up of a blend of ethanol, methanol, and isopropyl. Good luck >> finding what the percentages are. I've called one vendor before, and even >> they couldn't tell me. >> >> <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.avg.com_email-2Dsignature-3Futm-5Fmedium-3Demail-26utm-5Fsource-3Dlink-26utm-5Fcampaign-3Dsig-2Demail-26utm-5Fcontent-3Dwebmail&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=MiCOHRyehovZqvWLqUd85gtNyKdx2Qnkl8IyXhDVZ7A&e=> >> Virus-free. >> www.avg.com >> <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.avg.com_email-2Dsignature-3Futm-5Fmedium-3Demail-26utm-5Fsource-3Dlink-26utm-5Fcampaign-3Dsig-2Demail-26utm-5Fcontent-3Dwebmail&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=MiCOHRyehovZqvWLqUd85gtNyKdx2Qnkl8IyXhDVZ7A&e=> >> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> >> >> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet < >> histonet@lists.utsouthwestern.edu> wrote: >> >>> We use reagent alcohol on the processor and for staining. >>> >>> Get Outlook for >>> iOS<https://urldefense.proofpoint.com/v2/url?u=https-3A__aka.ms_o0ukef&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=G68TvqrXQ5gMkZ7yXrFjlTfSBXBWHSyCXohNpKIZAgI&e=> >>> ________________________________ >>> From: Jamie Watson via Histonet <histonet@lists.utsouthwestern.edu> >>> Sent: Saturday, May 12, 2018 9:06:01 AM >>> To: Histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Ethanol in tissue processing >>> >>> Hi All, >>> >>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol >>> or isopropyl alcohol on the tissue processor? >>> >>> Jamie >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= > > ************************************************************************************************ > MORPHISTO GmbH > PD Dr. phil. nat. Michael Gudo > Weismüllerstr. 45 > 60314 Frankfurt am Main > Telefon: 069 / 400 3019 - 62 > Telefax: 069 / 400 3019 - 64 > > E-Mail: michael.g...@morphisto.de <mailto:i...@morphisto.de> > Internet: > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.morphisto.de_&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=W1YneELMXXshKSQu4jJSvRn53FG5LC6-E-xdlQ4K4pQ&e= > > <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.morphisto.de_&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=W1YneELMXXshKSQu4jJSvRn53FG5LC6-E-xdlQ4K4pQ&e=> > > Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo > > Registergericht: Amtsgericht Frankfurt > Registernummer: HRB 74954 > Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: > DE243397199 > ************************************************************************************************ > Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder > dessen Vertreter bestimmt. 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