Thank you all for your answers, our RG alcohol is 3% methanol. You are confirming what I thought, we are using the same type of alcohol as most histology labs.
Jamie On May 13, 2018 7:37:52 AM "Jacox, Robert A. via Histonet" <histonet@lists.utsouthwestern.edu> wrote: > Jay, > > Michael's post is correct and very well stated. In the US we estimate > between 80 -85% of labs now use and RGA. The most common blend in the U.S > is 95-5 which is 95% ethanol denatured with 5% methanol. Any company you > purchase from should be happy to provide a C of A (certificate of analysis) > to verify how they denature. > > Hope this helps. > > Robert Jacox > Product Manager > Thermo Fisher Scientific > > Sent from my iPad > >> On May 12, 2018, at 5:21 PM, Michael Gudo (Morphisto GmbH) via Histonet >> <histonet@lists.utsouthwestern.edu> wrote: >> >> CAUTION: This email originated from outside of the organization. Do not >> click links or open attachments unless you recognize the sender and know >> the content is safe. >> >> >> Well, in Germany, and as far as I know in Europe, most labs use ethanol at >> 96 and 99 percent as the last two steps in tissue processing. These >> ethanols are are denatured with MEK (= Butanon, Methylethylketon). Methanol >> or isopropyl is not allowed as denaturation agent in Europe, otherwise you >> have to pay the full alcohol tax. >> >> In our lab we we use 96 % ethanol and as last step and then, before going >> to xylene we use isopropanol which has a better dehydration effect than >> ethanol. 99 % ethanol takes water from the air and quite fast becomes a 96 >> or 95 % ethanol and you will have some water left in the specimens when you >> to to xylene. >> So our experience are that the best quality of dehydration can be reached >> with ethanol as high as possible and finally isopropanol before the xylene >> (2 steps). >> >> With best regards >> Michael >> >> >>> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet >>> <histonet@lists.utsouthwestern.edu>: >>> >>> Most places use "reagent alcohol" which, from most suppliers, is a mongrel >>> blend of whatever is cheapest, and not even listed on the label. It's >>> usually made up of a blend of ethanol, methanol, and isopropyl. Good luck >>> finding what the percentages are. I've called one vendor before, and even >>> they couldn't tell me. >>> >>> <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.avg.com_email-2Dsignature-3Futm-5Fmedium-3Demail-26utm-5Fsource-3Dlink-26utm-5Fcampaign-3Dsig-2Demail-26utm-5Fcontent-3Dwebmail&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=MiCOHRyehovZqvWLqUd85gtNyKdx2Qnkl8IyXhDVZ7A&e=> >>> Virus-free. >>> www.avg.com >>> <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.avg.com_email-2Dsignature-3Futm-5Fmedium-3Demail-26utm-5Fsource-3Dlink-26utm-5Fcampaign-3Dsig-2Demail-26utm-5Fcontent-3Dwebmail&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=MiCOHRyehovZqvWLqUd85gtNyKdx2Qnkl8IyXhDVZ7A&e=> >>> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> >>> >>> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet < >>> histonet@lists.utsouthwestern.edu> wrote: >>> >>>> We use reagent alcohol on the processor and for staining. >>>> >>>> Get Outlook for >>>> iOS<https://urldefense.proofpoint.com/v2/url?u=https-3A__aka.ms_o0ukef&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=G68TvqrXQ5gMkZ7yXrFjlTfSBXBWHSyCXohNpKIZAgI&e=> >>>> ________________________________ >>>> From: Jamie Watson via Histonet <histonet@lists.utsouthwestern.edu> >>>> Sent: Saturday, May 12, 2018 9:06:01 AM >>>> To: Histonet@lists.utsouthwestern.edu >>>> Subject: [Histonet] Ethanol in tissue processing >>>> >>>> Hi All, >>>> >>>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol >>>> or isopropyl alcohol on the tissue processor? >>>> >>>> Jamie >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >> >> ************************************************************************************************ >> MORPHISTO GmbH >> PD Dr. phil. nat. 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