I was taught it best to use separate controls on a second slide. But in practice, the only cases where a floater appeared it was obvious. In my own lab 14 year experience it has not happened.
However, about 10 years ago we started an experiment and switched to 2 AFB slides with pos and neg controls on one slide only but with patients samples on both slides. Looking at 2 slides sometimes makes me more confident. I have not yet competed the study but anecdotally I do not recall or have never seen an AFB floater using this practice. We deliberately keep both our control blocks quite small maybe 1-2 mm. Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
