Hi Greg, It has been a problem for months across hundreds of kits. We added some washes and more dips in the DI water after the run to the protocol. It helps a little bit. I am going to try a more things in the next few weeks and I will keep the community updated.
-----Original Message----- From: Greg Dobbin <greg.dob...@gmail.com> Sent: Thursday, October 11, 2018 11:04 AM To: linda.marg...@cookchildrens.org Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Bond Max artifact Hi Linda, Is this a problem that has just started or has the red kit always had this artefact? - If it just started, perhaps it is a problem with a particular kit lot number (or numbers). If so, get Leica Tech service involved to see if anyone else has reported a problem. - If it has been an ongoing issue for the red kit then perhaps you need to modify the Red Kit protocol by adding additional (and/or longer) wash steps. Here again Tech Service can help you with this. - The only other thing I can think to ask is: Do you have anyone new operating the instrument? Could a new person be putting undiluted Bond Wash in the bulk container? Let us know what you find out. Good luck. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet