Especially a very blood tissue like GP spleen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com
________________________________ From: Patsy Ruegg <prueg...@hotmail.com> Sent: Thursday, January 31, 2019 11:51 AM To: Jan Shivers; histonet Subject: Re: [Histonet] guinea pig IHC In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin. What are others experiences with h202 blocking on frozen sections. I always used an IHC detection system that did not require h202 blocking for frozen sections. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com ________________________________ From: Jan Shivers <shive...@umn.edu> Sent: Tuesday, January 29, 2019 12:58 PM To: histonet Subject: [Histonet] guinea pig IHC Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet