Hello Taganrog, If you are having issues with high autoflorescence due to prolonged formalin fixation (7 days), it might help to do a few minutes extra protease digestion (5+ minutes) during tissue pretreatment. Alternately or in conjunction, I would suggest re-applying HER2 probe to the same slide that had already been pretreated and hybridized. By doing this a few times (even up to four tries), you will likely get FISH signals that you can see and enumerate.
Good luck, Mark Tarango On Tue, Jun 11, 2019 at 11:33 AM Пешков Максим via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > Dear colleagues! > Can anyone help? We are very appreciatated any suggestions, tips and hacks > for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more > than 7 days, then processed as usual into wax. IHC for Her2 has value as 2+. > Sincerely, > Maxim Peshkov, > Russia, > Taganrog. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet