Lori You can't use a dual label detection system. We have found most mouse polymers will cross react with porcine tissue. We know that the dako one does. You need to use the rabbit envision for rabbit monoclonal antibodies or with mouse monoclonals you need to use a rabbit anti-mouse and then a rabbit envision detection system.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com<mailto:l...@premierlab.com> www.premierlab.com<http://www.premierlab.com/> Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Lori Sorrells via Histonet <histonet@lists.utsouthwestern.edu> Sent: Wednesday, January 29, 2020 1:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Pig Tissues Good afternoon- Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues? I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies. I have used a variety of blockers including various sera in the diluents and blocking reagents. All samples are FFPE. I routinely use the DAKO EnVision+ Dual HRP system. Thanks in advance! Lori Sorrells Research Associate II Revivicor, Inc. 1700 Kraft Drive, Suite 2400 Blacksburg, VA 24060 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
