Yes, they can be left overnight in 95% alcohol before staining. We do at times and have not had any report of altered quality.
-----Original Message----- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, March 13, 2020 12:52 PM To: Histo List Subject: [EXTERNAL Sender] [Histonet] Pap slides This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- Can pap slides be left in 95% alcohol overnight to be stained the next morning without affecting the quality of the slides? Or should they be moved to xylene then run back prior to staining? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!t41b5SyZJvJtfmoB17JVt_hvFQ_Fgvuf42TYKsSF1BIUruucoQ1Qjmkduo-4wzUXruaX9w$ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet