It’s difficult to know without your full protocol, but you can easily test your DAB.
Take a paper towel and add a drop of your secondary/HRP. Add a drop of DAB to that. If it doesn’t immediately turn brown, your hydrogen peroxide has likely degraded into water. Make a fresh batch of DAB and try again. > On May 1, 2020, at 8:28 AM, Kristy Castillo via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hi Histonetters, > > We are starting our IHC (fun times), we are having trouble with the DAB > lighting up. Processed for 5 thru 10 minutes and still nothing. Our > permanent red is working just fine. Any ideas. > > Thank you! > > Kristy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet