Since you are newbies, I am just putting IHC 101 out there. Permanent red is for alkaline phosphatase detection and DAB is for HRP. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com
A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, May 1, 2020, 10:29:00 AM CDT, Kristy Castillo via Histonet <histonet@lists.utsouthwestern.edu> wrote: Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet