I would suggest to always refer to your reagent’s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. Our world is becoming more restrictive……
hope this helps, Lynette Pavelich, HT(ASCP), QIHC > On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Depends on what you are doing with the sections. > IHC or just dye -staining? > Sure...too hot ( cooking) is not recommended, as stated > Also stated is that high -temp fixation may also be used with no deleterious > effects as long as the fixation time is not extended. > However, RT -ish even for a week won't be a problem...imho > Needs must? > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
