Dear Carl, thank you so much for checking! Yes, may I please see some images? And would you mind to share your specific protocols and the brand also of the DAPI that you bought? (I am guessing it does not matter too much though). Thank you very very much, I really appreciate the help.
All the best Alida ps: my sections are demineralized bone, it should work the same for any tissue right? Do you recommend to cut and then stain right away? Or would it also work on paraffin slides that were cut a few years ago? On Wed, Jun 9, 2021 at 10:01 PM Hobbs, Carl via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Dear Alida > my emails to your msu.montan.edu get returned to me as undeliverable > I have incubated 2 dewaxed sections ( different slides) in HOECHST +/- > HIER. > Both stain nuclei very well. > The HIER-treated section gave a brighter nuclear positivity ( so needed to > lower exposure time on taking images) > NSD though > I can post on Histonet images, if you wish > > carl > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > > 020 7848 6810 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Dr. Alida M. Bailleul Associate Research Fellow/Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar <https://scholar.google.co.jp/citations?user=Yn4PuWMAAAAJ&hl=en> - ResearchGate <https://www.researchgate.net/profile/Alida_Bailleul> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
