In my experience soaking the block in water works just as well as surface decal. If you need to you can take the bone back, by reverse processing. Run the block through the clean cycle back to water, then leave the bone in NBF for 24-48hrs. The fixative will protect the tissue when you put it back into decal.
Gerard Spoelstra Sent from my iPad > On 1 Jul 2021, at 4:37 am, Patricia Latham via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > To Histopeeps, > Does anyone know if there is a method to decalcify bone once it is FFPE? > Thank you, > Pat L > George Washington University > Washington, DC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
