500 µg in 1000µl is the same as 100µg in 200µl So I would reconstitute the 100 µg with 200 µl of water to get the liquid antibody-concentrate. Then I would make the working-dilution: eg. 5µl of concentrate + 5000µl buffer
The correct way would be 5 µl + 4995 µl, but in practice this does'nt matter. Gudrun Lang -----Ursprüngliche Nachricht----- Von: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Donnerstag, 13. Oktober 2022 17:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Reconstituting antibodies and dilutions When calculating the dilution of an antibody when do start? i.e. If i have 100 ug of lyophilized antibody and its recommended reconstitution is 500 ug/ml How do I mix a 1:1000 dilution? I am thinking it's adding 2mL diH20 to create the 500 ug/mL and then diluting the sample. And I would be able to make 2000ml of 1:1000 concentration solution ? 2mL antibody and 1998mL diluent? Please let me know if I made a calculation error _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet