I just want to ask about precipitation reaction of secondary antibody especially on goat antibody conjugate Alexa dye 594 and 488 Invitrogen. I have noticed that the method I used using 1% BSA and 3% BSA blocking , then overnight with primary has reacted to my secondary goat anti Rabbit and mouse alexa dyre 594 and 488 with too much precipitation. I tried to remove it with PBS with 0.05 tween wash overnight and the precipitate are still there. Any help would be appreciated with my technique. Thank you so much.
Reuel Cornelia Cellular Pathology Laboratory Supervisor Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel. Number: 214-559-7766 FAX Number: 214-347-4806 Scottish Rite for Children is a world-renowned leader in the treatment of orthopedic conditions such as scoliosis, clubfoot, hand differences, hip disorders, sports injuries and fractures, as well as certain related arthritic and neurological disorders and learning disorders, such as dyslexia. Patients receive treatment regardless of the family's ability to pay. For more information about services available at our Dallas or Frisco campuses, visit scottishriteforchildren.org. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
