Same here, we moved to a new location and the PH of the water was too acidic, 
the DI water would remove the silver and leave it yellowed.   Some solutions 
that I was making that usually would take hours to go into solution on the stir 
plate went immediately.

Good Luck!

Ginny Kurth, HT, (ASCP)CM


-----Original Message-----
From: Bacon, Charles via Histonet <histonet@lists.utsouthwestern.edu>
Sent: Saturday, September 16, 2023 2:15 PM
To: Steven Crochiere <scrochi...@hotmail.com>
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retic help

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Hi Steven,

We ran into this issue and it was the pH of the water. We were using our 
sterile line from the facility but the pH was too high. We used tap and it 
worked fine. This will also effect your stock solutions if you make them 
yourself so be aware of that as well.

Sent from my iPhone

> On Sep 15, 2023, at 4:02 PM, Steven Crochiere <scrochi...@hotmail.com> wrote:
>
> Any advice on getting my retic stain to work properly is appreciated.
> Currently coming out a washed out gray overall, with proper staining
> at the periphery of the section. Thanks
>
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