Why would one decide to use a primary antibody along with a secondary antibody rather than a primary antibody conjugated with the secondary?
Example. I have a researcher who wants to do CD11C staining with Alexafluor488 Is it better to buy and use a primary antibody CD11C conjugated with Alexafluor488 or to do the CD11C primary and a Rabbit anti-rat (H_L) IgG antibody secondary? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
