Awesome thank you Val. I like the multi control option. Can save money in the end by using less reagents
On Wed, May 8, 2024 at 10:52 AM Val T <vtolle...@gmail.com> wrote: > Hey Charles- > > Our research lab prepares a “multimouse” control. We embed 10 organs on > the same slide and it serves as a +/- control for just about every IHC > marker. > However, if you don’t want to do that- here are some tissues that would > make nice controls. > > > BCL-2- spleen, lymph nodes > > Caspase-3- spleen, lymph nodes > > CD117- skin, spleen, brain, lymph nodes > > CD62E (e-selectin), bone marrow, colon > > Collagen II, cartilage > > Desmin, heart, skeletal muscle > > GFAP, brain, spinal cord > > HMB45, skin > > Ki67, spleen, bowel > > p53, tumors, colon > > s100, colon, muscle > > Brain can serve as a negative control on just about all these. CD117 will > have some expression in the cerebellum, but will be negative in most other > areas. > Heart can be a negative control for GFAP. > Val > > > On May 8, 2024, at 6:59 AM, Charles Riley via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > Hello all, > > > > I am trying to put together a positive and negative tissue control list > for > > the following antibodies at my research core. I came over from a > clinical > > pathology background and we used tonsils for a lot of these antibodies. > > Since rats and mice don't have tonsils I am looking for something they do > > have that can be used if possible to keep the controls as similar to the > > samples as possible. > > > > BCL-2 > > Caspase-3 > > CD117 > > CD62E (e-selectin) > > Collagen II > > Desmin > > GFAP > > HMB45 > > Ki67 > > p53 > > s100 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet