Dear MARMAMs,

My co-authors and I are happy to share our note on delphinid postmortem 
vitreous humor biochemistry, available online at:
https://onlinelibrary.wiley.com/doi/epdf/10.1111/mms.13157.

Please let me know if you cannot access the document, as I am happy to share a 
.pdf with anyone interested.

Zorotrian, T., Stern, A. W., Costidis, A., Harms, C. A., Gao, H., Fontaine, C., 
& Adams, H. R. (2024). Delphinid postmortem vitreous humor biochemistry as 
proxy for antemortem blood analyte concentrations. Marine Mammal Science, 
e13157. https://doi.org/10.1111/mms.13157

Biochemical analysis of vitreous humor has multiple forensic applications in 
human death investigations, including estimation of postmortem interval and as 
proxy for select antemortem serum/plasma analytes. Little work has been done on 
vitreous humor biochemistry in cetaceans due in part to a paucity of 
appropriate cases and relatively high cost of analysis. To investigate the 
utility of vitreous humor biochemistry in stranded delphinids, paired 
antemortem serum/plasma and postmortem vitreous humor samples (n=10) were 
analyzed on the UF Clinical Pathology Lab’s Beckman Coulter AU480. Results were 
assessed via a Wilcoxon signed-rank test and calculation of the intraclass 
correlation coefficient.  This study supports the utility of postmortem 
biochemical analysis of delphinid VH for data on antemortem health status and 
metabolic state. Analytes that are likely to be informative for forensic 
purposes or to provide insight into antemortem health include UN, CREA, GLU, K, 
Na, and Cl.

Sincerely,
Tiffany

​Tiffany J. Zorotrian, MSc, LVT, CVT
PhD Student
Aquatic Animal Health Program
Large Animal Clinical Sciences
College of Veterinary Medicine
University of Florida
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