[Histonet] Toluidine Blue and staining archaeological residues.
Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which are lifted using 20ul of water, the subtle colour differences between blues, purples, and violets have not been useful. I was looking at the possibility of counterstaining the Toluidine Blue stained residues with say Phloroglucinol or IKI which would highlight the plant component of the residue and then see if what was left looked like collagen. Has anyone tried this type of counterstaining? OR does anyone know of a stain that would colour differently for plant and animals understanding that this is not a tissue section but microscopic residues in solution? Thanks Birgitta Stephenson The Research Microscopy Laboratory, University of Queensland. -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - Access your email from home and the web ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] vanGieson-Trichrome staining protocol
And it looks so pretty (VVG/TRI)- especially on lung. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rena Fail Sent: Monday, July 19, 2010 12:47 PM To: Doughty, Adele; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] vanGieson-Trichrome staining protocol Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then a trichrome it can be done. The silver should be cloudy like a slightly dirty window , not milky generally if you add enough ammonia to the silver and sodium hydroxide mixture to render it completely clear then you will probably have added too much ammonia. Rena Fail - Original Message From: "Doughty, Adele" To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] vanGieson-Trichrome staining protocol
Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then a trichrome it can be done. The silver should be cloudy like a slightly dirty window , not milky generally if you add enough ammonia to the silver and sodium hydroxide mixture to render it completely clear then you will probably have added too much ammonia. Rena Fail - Original Message From: "Doughty, Adele" To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Wax removal
Hi, Thanks for the reminder. We purchased them from Lab Safety Supply. They are called Safety Treads, and they come in a variety of sizes and shapes. The one we have is 6 X 24 in (I guesstimated the size wrong earlier) and it's part number is 10766BR. Here is a link to the web page for them: http://www.labsafety.com/search/10766/24527943/?GoButton=Go I hope this helps, it has worked great for us! Oh and getting them to sweep more regularly helped too, we were having a lot of trouble with dust. Happy Monday, Amos On Mon, Jul 19, 2010 at 10:31 AM, Hartz, Rhonda SktnHR < rhonda.ha...@saskatoonhealthregion.ca> wrote: > I would appreciate that info. > > Thanks. > > > Rhonda Hartz > Technologist Supervisor > Anatomic Pathology Division > Saskatoon Health Region > (306) 655-8197 > rhonda.ha...@saskatoonhealthregion.ca > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos > Brooks > Sent: July 16, 2010 7:04 PM > To: Hartz, Rhonda SktnHR; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wax removal > > Hi, > You can get abrasive pads that have adhesive on one side. They are > usually used on stairs. They are like 8 inch wide 3 foot long strips of > sandpaper that stick (really well) to the floor. I have them placed > about 1 per foot along main traffic areas. I don't recall off the top of > my head where we got them from, but if you'd like, I'll look it up on > Monday. > > Amos > > > Message: 6 > Date: Thu, 15 Jul 2010 10:42:26 -0600 > From: "Hartz, Rhonda SktnHR" > Subject: [Histonet] Wax removal > To: > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi everyone; > > This may seem like a insignificant question, but we consistently have > issues with injuries to our maintenance staff from cleaning the wax off > of our floors. Housekeeping has laid sticky layered mats all over our > floors (like large mouse traps), which they peel off layer by layer as > they become wax covered. Apparently these are very expensive. Does > anyone have any suggestions? > > Rhonda Hartz > Technologist Supervisor > Anatomic Pathology Division > Saskatoon Health Region > (306) 655-8197 > rhonda.ha...@saskatoonhealthregion.ca > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] vanGieson-Trichrome staining protocol
Adele, Since the vanGieson IS a trichrome stain, why would anyone wish to combine one trichrome with another? Bryan - Original Message - From: "Doughty, Adele" To: Sent: Monday, July 19, 2010 10:29 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] vanGieson-Trichrome staining protocol
Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] human on human IHC
Anything using FITC is pretty expensive. If you aren't want= ing
flourescence then why would you use this? Does this specific conj
ugation eliminate the Fc binding? Conjugating to HRP doesn't seem to
= yield that Fc region staining that I can see? Just a happy Monday
que= stion. =)
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
<= /span>
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 = Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: Re: [Histonet] human on human IHC
From: [1]andreahoo...@rocketm= ail.com
Date: Mon, July 19, 2010 6:06 am
To: [2]sgoe...@xbiotech.com, "Ki= m Merriam" <[3]kmerriam2...@yah
oo.com>
Cc: "Histonet" <[4]h= isto...@lists.utsouthwestern.edu>
Along those same lines I have conjugated primaries to biotin and it
works b= eautifully for human on human IHC. Another alternative if
extra amplificati= on is needed is to conjugating to FITC and then
using a rabbit-anti-FITC fo= llowed by an anti-rabbit polymer.
Just be aware with human on human controls are imperative as you may
get bi= nding to human Fc receptors.
Andrea Hooper
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: <[5]sgoe...@xbiotech.com>
Sender: [6]hist= onet-boun...@lists.utsouthwestern.edu
Date: Thu, 15 Jul 2010 09:27:19
To: Kim Merriam<[7]kmerriam2003= @yahoo.com>
Cc: Histonet<[8]hist= o...@lists.utsouthwestern.edu>
Subject: RE: [Histonet] human on human IHC
I am doing alot with this right now. The best thing I havefound to
do is to conjugate your primary antibody with HRP, then just use
the direct method (no secondary), and it turns out beautifully!!
You can buy thekit to do this from Fisher. I can get the exact
order number if you need it. Good Luck!!
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] human on human IHC
From: Kim Merriam <[1][9]kme= rriam2...@yahoo.com>
Date: Thu, July 15, 2010 8:00 am
To: Histonet <[2][10]histo...@lists.utsouthwestern.edu>
Hi,
Does anyone know of a human-on-human IHC kit similar to the
mouse-on-mouse kits
that are available?
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
___
Histonet mailing list
[3][11]histo...@lists= .utsouthwestern.edu
[4][12]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3D"[13]mailto://kmerriam2...@= yahoo.com"/
2. 3D"[14]mailto://hi= sto...@lists.utsouthwestern.edu"/
3. 3D"[15]mailto://Hi= sto...@lists.utsouthwestern.edu"/
4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
___
Histonet mailing list
[16]histo...@lists.utsou= thwestern.edu
[17]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3D"mailto://andreahoo...@rocketmail.com"/
2. 3D"mailto://sgoe...@xbiotech.com"/
3. 3D"mailto://kmerriam2...@yahoo.com"/
4. 3D"mailto://histonet@lists.utsouthwestern.edu"/
5. 3D"mailto://sgoe...@xbiotech.com"/
6. 3D"mailto://histonet-boun...@lists.utsouthwestern.edu"/
7. 3D"mailto://kmerriam2...@yahoo.com"/
8. 3D"mailto://histonet@lists.utsouthwestern.edu"/
9. 3D"mailto://kmerriam2...@yahoo.com"/
10. 3D"mailto://histo...@lists.utsouthwestern.ed=/
11. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; 13.
3D"mailto://kmerriam2...@yahoo.com"/
14. 3D"mailto://histonet@lists.utsouthwestern.edu"/
15. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
16. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
17. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
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Histonet mailing list
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Re: [Histonet] human on human IHC
Along those same lines I have conjugated primaries to biotin and it works
beautifully for human on human IHC. Another alternative if extra amplification
is needed is to conjugating to FITC and then using a rabbit-anti-FITC followed
by an anti-rabbit polymer.
Just be aware with human on human controls are imperative as you may get
binding to human Fc receptors.
Andrea Hooper
Sent from my Verizon Wireless BlackBerry
-Original Message-
From:
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Thu, 15 Jul 2010 09:27:19
To: Kim Merriam
Cc: Histonet
Subject: RE: [Histonet] human on human IHC
I am doing alot with this right now. The best thing I havefound to
do is to conjugate your primary antibody with HRP, then just use
the direct method (no secondary), and it turns out beautifully!!
You can buy thekit to do this from Fisher. I can get the exact
order number if you need it. Good Luck!!
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] human on human IHC
From: Kim Merriam <[1]kmerriam2...@yahoo.com>
Date: Thu, July 15, 2010 8:00 am
To: Histonet <[2]histo...@lists.utsouthwestern.edu>
Hi,
Does anyone know of a human-on-human IHC kit similar to the
mouse-on-mouse kits
that are available?
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
___
Histonet mailing list
[3]histo...@lists.utsouthwestern.edu
[4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3D"mailto://kmerriam2...@yahoo.com"/
2. 3D"mailto://histonet@lists.utsouthwestern.edu"/
3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lyve - 1 on Bond Max
** Proprietary ** ** Reply Requested When Convenient ** Hi there, I was wondering if anyone on the histonet list use anti-mLYVE-1 antibody (purified in rat Monoclonal IgG 2A from R&D System) on the Bond Max. We have been trying out various titrations and pre-treatment without much success. All we seems to achieve is background staining with no real specificity at 1:100 H1(10) ; 1:100 H2(20) ;1:100 E2(10): 1:50 E1(30) ;1:50 H1(30); 1:50 H2(10) Any suggestion ? Regards Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk * This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Apoptosis staining on mouse testis
Hello all, I am trying to stain for apoptosis on bouins fixed, paraffin embedded mouse testis. I have tried a cleaved caspase-3 anitbody, & TUNEL, with no success. Any suggestions would be much appreciated. Many thanks Lindsey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
