[Histonet] Bringing new antibodies in house - Ki-67,P40,PAX8
Hello Everyone, I am currently looking to bring a few new antibodies in house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and wanted to get some opinions from everyone. For P40 and PAX8; are you using a monoclonal or polyclonal? Also what company are you getting them from? My hospital deals mainly with Dako, Biocare, and Cellmarque. We are a very small facility that does HIER with a waterbath, and then manual IHC pipetting with the old sequenza coverplate tiles. So usually we prefer concentrate for antibodies not pre-dilute because of the longer expiration times, and of course the cost. So I guess what I am asking is, what clone do you prefer. What company do you get it from? How much does it cost? And have you ever run it on a manual setup, if so how was it to work with? Thanks for all the help in advance, Natalie J. Nagy Histology Supervisor Holyoke Medical Center nagy_nata...@holyokehealth.com CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
histonet@lists.utsouthwestern.edu
Dear Laurie, We use thrombin and plasma to form our cell blocks. We order the Topical Thrombin 5,000 IU from King Pharmaceuticals. Hope this helps, Natalie Nagy (HT)ASCP Holyoke Hospital Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about slide drying/ convection ovens
Hi everyone, Just have a quick question for all of you out there in histo-land. Have you ever bought or worked with a gravity convection oven (the thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly dry all of your slides, did it keep temp?? I am thinking about buying one, and wanted some opinions. Or do you all prefer a real forced air slide drying oven? Thanks in advance, Natalie Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help with CPT code 88363 for archived tissue retrieval
Hi everyone, I just have a question about CPT code 88363, first can it be used for pulling blocks for Oncotype DX testing, also is there a time limit on when this code can be used? Does it have to be within a year, a month, etc...of when the patient account went active? Thanks for all the help, Natalie J. Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet