[Histonet] HHV8 Antibody

[Rae Staskiewicz via Histonet]

Has anyone had experience with this antibody? One of our Hemepaths wants to
bring this on line. Where do you get your antibody and what is your
protocol? Any info would be much appreciated.

 

Rae Staskiewicz

UnityPoint Health-Methodist/Proctor

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[Histonet] C-Myc

[Rae Staskiewicz]

Would anyone have a protocol for C-Myc using the Ventana Benchmark Ultra or
XT? Thanks in advance for any information you can provide.

 

Rae Ann Staskiewicz

UnityPoint Health Methodist

Peoria, IL

309-672-5994

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[Histonet] H pylori and Ventana instruments

[Rae Staskiewicz]

Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori 
antibody you are using, and whether or not you are experiencing non-specific 
staining, or staining that looks specific but with a stippled effect. 

Thanks

Rae Staskiewicz
UnityPoint Health-Methodist


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[Histonet] FW: processing after Bouin's fixation

[Rae Staskiewicz]

Sending this for a friend, please do not reply to me.

 

  _  

From: Burton, Lynn [mailto:lynn.bur...@illinois.gov] 
Sent: Thursday, October 18, 2012 8:43 AM
To: raest...@grics.net
Subject: FW: processing after Bouin's fixation

 

Rae,

I have been trying to get this out on Histonet and I can't seem to address
it correctly. It is for Dr. Dybas. Would you please help me?

Lynn

 

From: Burton, Lynn 
Sent: Wednesday, October 17, 2012 3:14 PM
To: 'histo...@lists.utsouthwester.edu';
'histonet-boun...@lists.utsouthwestern.edu'
Subject: FW: processing after Bouin's fixation

 

 

 

From: Burton, Lynn 
Sent: Wednesday, October 17, 2012 1:46 PM
To: 'on behalf of Lynn Burton'
Subject: processing after Bouin's fixation

 

Hi,

I have a question about the step in between Bouin's fixation and alcohol
dehydration of rat brain tissue, nucleus accumbans. Several procedures are
suggested by various resources, what are any of you using successfully?

Lynn Burton

Animal Disease Lab

Galesburg,Il

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[Histonet] ADH 5

[Rae Staskiewicz]

Is anyone doing the ADH 5 stain on a Ventana platform?

Rae Staskiewicz
Methodist Medical Center in Illinois


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[Histonet] QIHC

[Rae Staskiewicz]

Is there any other resource for information and studying for the QIHC exam
other than the ASCP outline?

 

Rae Staskiewicz

 

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[Histonet] H Pylori Control

[Rae Staskiewicz]

Good Afternoon everyone,

 

My institution is about to start running H Pylori for another lab. They
microwave process their tissue. Would there be anyone out there who
microwave processes and would have one or two blocks of H Pylori positive
control tissue they would  be willing to let us have?

 

Thanks in advance,

 

Rae Ann Staskiewicz

Methodist Medical Center of Illinois

Peoria, IL

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RE: [Histonet] NCCI policy on IHC billing

[Rae Staskiewicz]

Clair,

I agree that the cocktailed antibodies would be charged as a single test,
however, with the dual and triple stains, since these are technically done
with separate procedures, would not each antibody be able to be charged?
This is what we are thinking. How are others interpreting this

Rae Staskiewicz

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare
Thornton
Sent: Thursday, January 05, 2012 5:35 AM
To: Sally Price; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] NCCI policy on IHC billing

Sally,

The way I read it, I don't think it's one antibody per specimen; I think
what they are saying is that if you are running one antibody on multiple
blocks from one specimen, you can only bill once for that antibody.  I think
if you are running a panel of antibodies one one block from a specimen, you
can still charge for each antibody.  However, I completely agree with you.
This change came as a big surprise for our lab, until Dorothy posted it on
Histonet we had no idea.  How can that be?  (And thanks, Dorothy!)  The day
that post came out we happened to be running 20 pankeratin/p63 double stain
slides, all on multiple blocks from one specimen.  The two antibodies from
that double stain are applied at different times (to the same slide) and we
use two different detections.  So had we ran them after the new year, we
would've been able to charge only once for those 20 slides, never mind 20
tests being used from each antibody and 20 tests being taken from two
different detection kits.  And the tech time put into cutting those 20
slides!  We have several double and triple stains that we run on a daily
basis, and only one component from the triple stain is a cocktail; the rest
are separate antibodies being applied to the same slide using two different
detections.  I'm not sure exactly what our lab is going to do about it, but
somehow this change should have been made aware to everyone well before it
happened.  There are going to be labs who are no longer in compliance, and
we all know what that means..



Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com

From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sally Price
[sprice2...@gmail.com]
Sent: Thursday, January 05, 2012 12:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] NCCI policy on IHC billing

Histonetters:
I waited a few days to see how others might weigh in after this information
was posted.  Call me crazy, but I expected quite a bit more reaction from
our community.  How is it that such a signifcant change in how IHC testing
may be conducted and will be paid for in the future can produce so little
response?

The way this new policy is stated, it looks pretty straightfoward: one
antibody (IHC procedure) per specimen; so, when it's necessary to use a
battery of IHC stains to determine the origin of an undifferentiated
neoplasm, the lab can only bill for one procedure.  How could such an
approach be possible?  And what about multi-antibody procedures, which
are usually more cost effective than single-antibody procedures?

Come on folks, this is a big deal becuase IHC staining is essential to to
the practice of anatomic pathology and provides a lot of us with our
livelihood.  I know I'm not alone in thinking that the CMS needs to know
that this new policy is completely impractical and must be changed.  Sure,
there's some unnecessary IHC procedures being performed, but this isn't the
way limit the problem.
Sally
--

Message: 6
Date: Fri, 30 Dec 2011 12:33:17 -0600
From: "Webb, Dorothy L" 
Subject: [Histonet] NCCI policy update
To: "'histonet@lists.utsouthwestern.edu'"

Is everyone aware that beginning 1/1/12, we can no longer bill for each
block regarding IHC billing, only one unit of billing for each part type no
matter how many blocks are stained? Also IHC "cocktail" stains, such as
PIN4 must now be billed as one unit even though multiple antibodies are
reported out.

Kind of a surprising reversal of the policy set in motion 10/1/2009.
SPECIMEN becomes the unit of service rather than block(s) for IHC codes
88342, 88360, and 88361.

Happy New Year to everyone out there. May 2012 find you happiness and
health!

Dorothy Webb, HT
Regions Histology TS
651-254-2962
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RE: [Histonet] AMPHYL

[Rae Staskiewicz]

Linda, 

Where do you get your Lysol IC?

Rae Staskiewicz

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Thursday, August 25, 2011 1:07 PM
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] AMPHYL

We use Lysol I. C.; haven't be able to get Amphyl for years. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
barbara.cr...@lpnt.net
Sent: Thursday, August 25, 2011 12:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AMPHYL

My materials management department told me that AMPHYL has been
discontinued.
Ventana recommends that we clean/decontaminate the Benchmark and the
Ultras with AMPHYL.

Has anyone else ran across this?  Has AMPHYL really been discontinued?
Is there a substitute we can use.

Thanks everyone!


ANTOINETTE CRILL
ANATOMIC PATHOLOGY
EXT 5451

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RE: [Histonet] Outdating- Embedding Wax and Mounting Media

[Rae Staskiewicz]

Now you guys have done it!!! All the QAQC gurus are thinking!!!

Rae Staskiewicz

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Saturday, May 14, 2011 4:56 PM
To: John Smallwood; Histonet
Subject: Re: [Histonet] Outdating- Embedding Wax and Mounting Media

How funny! You are so right!

What about the ink on all of those old reports and reference books? Isn't
ink a 
dye in solvent?

The best special stains I ever did were back in the day when I made my own 
solutions 

from dry chemicals that were already 20 to 30 years old.

Paperwork, just a way to decrease productivity! 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com





From: John Smallwood 
To: Histonet Submissions 
Sent: Sat, May 14, 2011 4:18:36 PM
Subject: [Histonet] Outdating- Embedding Wax and Mounting Media

  To all Quality Control Purists
There seems to be a growing concern with regards to outdating on reagents
and 
their  continued use beyond this date.  I notice that Histological wax and 
Mounting media are dated with an expiry date. Just begging the
question-should 
we go back. melt down and re-embed all old blocks in fresh wax ?? Should we 
recover slip old slides in fresh dated Mounting media. With all the fuss
over 
quality control in the Laboratory and Government Lab Inspection oversight ,
how 
can this be ignored and our archives put at such dreadful risk ??

J. Smallwood,MLT.
STEGH
St.Thomas, Ont. 
Can.
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[Histonet] Competancies for handling hazardous material

[Rae Staskiewicz]

 

Has anyone been inspected by the EPA regarding removal of hazardous
chemicals?  It came up in a safety meeting that I should have training and
competencies (beyond general safety training and PPE) for pouring xylene
from staining dishes and processors into accumulation containers.  

 

Rae Ann Staskiewicz

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[Histonet] Contact information

[Rae Staskiewicz]

Could anyone send me the contact information for Charlie Doerner?

 

Rae Ann Staskiewicz

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[Histonet] Pax 8

[Rae Staskiewicz]

Does anyone know where to purchase Pax 8 antibody for formalin fixed paraffin 
embedded tissue?

Rae Staskiewicz
Methodist Medical Center


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[Histonet] Cutting Microarrays

[Rae Staskiewicz]

 

Good Morning All,

 

I am having some issues when cutting a microarray containing 4mm punches.
The punches are rolling and separating from the paraffin during cutting.
Anyone have any tips or ideas??

 

Rae Staskiewicz

MMCI

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RE: [Histonet] Lung biopsies - Question

[Rae Staskiewicz]

We cut 10 unstained slides.

Rae Staskiewicz
Methodist Medical Center of Illinois

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Thursday, August 26, 2010 3:54 PM
To: Histonet
Subject: [Histonet] Lung biopsies - Question

How many labs cut extra tissue sections (or tissue curls) for molecular
testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of
H&E sectioning)?  Thanks.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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RE: [Histonet] RE: Barcode and Tracking Information

[Rae Staskiewicz]

Jan,

Ditto! Ditto! Ditto! Couldn't have said it better myself!

Rae Staskiewicz

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Wednesday, May 05, 2010 2:53 PM
To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu
Cc: histonet-requ...@lists.utsouthwestern.edu;
":histonet-bounces"@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] RE: Barcode and Tracking Information

Blake,
I think I put in my comments about Ventana's Vantage when you first posted
the question, but here goes again..  It is wonderful.  Vantage is the best
thing to come along in my lifetime as a histo tech!  Wonderful for patient
safety, easy for the techs to use and a manager's dream for all the data it
can provide.
Jan Mahoney
Omaha, NE

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maggie Allen
Sent: Wednesday, May 05, 2010 2:24 PM
To: histonet@lists.utsouthwestern.edu
Cc: histonet-boun...@lists.utsouthwestern.edu;
":histonet-bounces"@lists.utsouthwestern.edu;
histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] RE: Barcode and Tracking Information

Hello Blake,

LabelClinic HTS is a tracking system for Histology artifacts within the
Pathology workflow. The product is designed to provide improved quality
control and traceability with the lab. It provides flexible and reliable
labeling and tracking of histology requisitions, containers, specimens,
blocks and slides. Using bar code technology, LabelClinic HTS provides an
optimized workflow that reduces errors and significantly enhances patient
safety.  It can be used stand alone, or integrated into an LIS / AP software
system.

Benefits:
*   Flexible lab configuration for custom lab workflow
*   Reduce errors and increase efficiency
*   Just-in-time reporting of artifact locations or last know location
*   Alerting for sub-process time violations and missing artifacts
*   Deploy to Desktop PC's or mobile hand held's
*   Configurable workflow
*   Supports color selection for slide and cassette printers

I would be happy to set up an online WebEx demo of the system for anyone who
may be interested. Thank you!


Maggie Allen
Healthcare Business Development Manager
Niceware International, LLC
200 South Executive Drive
Suite 200
Brookfield, Wisconsin 53005
Tel  (810) 629-3930
Cell (215) 200-0268

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[Histonet] Illinois Annual Meeting

[Rae Staskiewicz]

This year the Illinois Society for Histotechnologists is holding their
Annual Spring meeting at Eaglewood Resort in Itaska, Illinois. The dates are
May 13-14, 2010. Unfortunately it seems as though we are trying to follow
the example of the Republican National Committee and have an incorrect phone
number for room reservations at the resort. The correct number is
1-877-285-6150. Be sure to mention that you are with the ISH when making
your reservation in order to receive the convention rates. Reservations must
be made by April 12 in order to receive the reduced rates. Check out the
Eaglewood Resort at www.eaglewoodresort.com
  to see all of the amenities waiting for
you! If you would like an electronic brochure, contact myself at
raest...@grics.net. 

 

 

Rae Ann Staskiewicz

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[Histonet] Her-2 Controls

[Rae Staskiewicz]

Morning All,

 

My chief pathologist has asked the following question. For all of those
doing Her-2 Neu staining for scoring with the Vias system. How many control
slides do you cut in advance and how long are they viable if kept
refrigerated?  

 

Thanks in advance,

 

Rae Ann Staskiewicz

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[Histonet] Poor Nuclear Detail

[Rae Staskiewicz]

We had a case the other day which showed poor nuclear detail. This was fatty
breast which had been fixed overnight before processing. Tissues were
reprocessed in hopes of improving, but failed to do so. Does anyone have any
ideas what may have caused this artifact. All other cases that day were
fine.

 

Rae Ann Staskiewicz

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[Histonet] FW: Commercial Source for Direct Immunofluorescence Control Tissue

[Rae Staskiewicz]

Forwarding this for someone else.

Rae Staskiewicz

 

  _  

From: Dana Spears [mailto:dspe...@mmci.org] 
Sent: Monday, April 06, 2009 3:11 PM
To: histonet-requ...@lists.utsouthwestern.edu
Subject: Commercial Source for Direct Immunofluorescence Control Tissue

 

Anyone know of a commercial source for FITC Control tissue?  I've searched
the archives and saw the question asked before, but no answer!

 

Thanks to all who respond!

 

Dana Spears, HTL(ASCP)
Laboratory Manager
Methodist Medical Center
(309) 672-4930 (office)
(309) 255-7214 (cell)
(309) 279-3768 (fax)
dspe...@mmci.org


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[Histonet] Fall Symposium

[Rae Staskiewicz]

The Illinois Society for Histotechnologists is holding a one day seminar on
November 8, 2008 at the Four Points by Sheraton in Fairview Heights, IL near
St. Louis, MO. Get 2 workshops (6 CEUs) for only $35!  Registration includes
lunch.  You can register on-line at www.ilhisto.org
 , or register on the day for only $10.00
additional.

Workshops being offered are:

 

Basic Dynamics of Fixation and Processing - Herbert Skip Brown

 

Where Do I Find that Antibody and What Do I Do Once I have it? - Charlie
Dorner

 

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