RE: [Histonet] Request for Coverslip Removal SOP
We put xylene in a jar, add the slide, cover the jar loosely and put it in a warming oven. Alternately, you could put the jar in a water bath. The warm xylene helps to soften the old mounting media. This could take some time though . old mounting media can be some pretty tough stuff! ;o) Good luck! Michelle From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Friday, January 14, 2011 3:15 PM To: 'Sean McBride'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Request for Coverslip Removal SOP Hi, I don't have an actual SOP but we just soak in a jar of xylene until the coverslip come off. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Friday, January 14, 2011 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Request for Coverslip Removal SOP Importance: High Hi colleagues, I need to remove a glass coverslip from a mounted H&E biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1191 / Virus Database: 1435/3378 - Release Date: 01/13/11 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Request for Coverslip Removal SOP
Hi, I don't have an actual SOP but we just soak in a jar of xylene until the coverslip come off. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Friday, January 14, 2011 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Request for Coverslip Removal SOP Importance: High Hi colleagues, I need to remove a glass coverslip from a mounted H&E biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Request for Coverslip Removal SOP
soaking in xylene for at least a couple of hours (overnight is best) works for us. On Fri, Jan 14, 2011 at 11:55 AM, Sean McBride wrote: > Hi colleagues, > > I need to remove a glass coverslip from a mounted H&E biopsy slide with an > implant in order to run some biomaterial surface characterization studies. > Does anyone have a SOP that they would be willing to share for removing > glass coverslips without damaging the specimen? Thanks in advance for all > of the great advice that I always get from the histonet. > > > Best regards, > > > ~Sean McBride > > > Scientific Specialist > Bone Tissue Engineering Center > Carnegie Mellon Research Institute > Suite 4311 > 700 Technology Drive > Pittsburgh, PA 15219-3124 > > 412-268-8275 (o) > 412-915-1683 (m) > 412-268-8275 (fax) > smcbr...@andrew.cmu.edu > > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
