The fixation time is too short.
Fix as long as you can (how about 2-3 days at least for brain?)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and
Hello all,
I have received embolic material in test tubes in 10% formalin. The
tubes had been sitting around for about 2 years. To quantitate the
number of embolic particles, after removing particles at less than 40
microns, the solution was poured into a petri dish and th
Ethanol/alcohol is what will process the specimen. If the tissue is fixed
would it really matter that the tissue came into contact with ethanol?
Mark
On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) <
randi.ha...@horizonnb.ca> wrote:
> At a recent conference, our PA learned of using GE
I was wondering if anyone could help me find a supplier for Ketamine. We
usually order ours from Sigma but they are back ordered until mid September. I
have considered trying to order Ketamine/Xylazine solution in place of the
powdered forms but I don't know what ratio to get. We use this for
Nueterra, a leading developer and manager of physician-owned surgery
centers and specialty hospitals has an immediate opportunity available
for a Histotechnician.
Great opportunity for a Histotechnician in a brand new laboratory!
Nueterra Pathology Services in Irving, TX is looking for a
Sounds like to me that it might involve another revalidation of each one of
your antibodies.
Good luck
On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) <
randi.ha...@horizonnb.ca> wrote:
> At a recent conference, our PA learned of using GEWF (glacial acetic acid,
> ethanol, distilled w
At a recent conference, our PA learned of using GEWF (glacial acetic acid,
ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node
retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering
how "safe" it is to use when staining for IHC. Does anyone hav
A friend is asking this question and we were looking for responses
telling what others would do in this situation:
I am hoping to submit some brains for paraffin-embedded sectioning
soon, but we started intending to go the frozen-section route, so I
have some questions as to the feasibility
Dear Histonetters,
We were asked to process Hamster tissue(Large intestine) for one of our
investigators. I would like to know whether any of our users process hamster
tissues in their labs, if so kindly provide me a protocol for the same. Our
tissue processor is Sakura VIP6.
Thanks for all
Everyone,
Thanks for your help, based off your explanations, statements and citations,
we will attempt to protest this and have it expunged.
On Mon, Jul 26, 2010 at 4:41 PM, Lee & Peggy Wenk wrote:
> See if you can get the following article. Biotech Histochem is published by
> the Biological S
We keep our water bath at 40-41 C. Sometimes we add some 70% ethanol to the
water bath and this helps to get the wrinkles out (especially for cerebellum
and mice brain). Most of our tissue is bovine brain but we deal with multiple
other species including mice. We use McCormick Paraplast Plus
My water bath is usually around the same temperature as yours is.= I
just take literally a hand full of ice and put it in the bath, onc= e
all the ice melts, I cut the brain. I'll try to go look at what tem p
it gets to, my guess would be around room temperature? Your
To All:
We have been experiencing problems sectioning mouse brain. The sectioning is
fine, but we have problems in the water bath. At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections
Try cooling down your water bath with ice before you cut the brai= n.
All CNS tissue will act like you described. You may end up w= ith
more wrinkles with the cooler bath, but put the slides in a 70C oven
fo= r about an hour and they will usually fall out. =)
Happy Cuttin=
How big are these rat brains? Are you processing by hand or with a machine?
We routinely process pieces of human brain for about 10 to 11 hours on a VIP.
This seems like a really really long cycle. No heat and no vacuum until the
paraffin infiltration step. (formalin, 70% EtoH, 95% EtoH, 95%
Hi there,
I am currently using rat brain for histology but something is not working. I
follow the protocol below for tissue processing.
Station
Solution
Routine (mins)
1
Formalin
10
2
Formalin
10
3
70% Ethanol
60
4
80% Ethanol
240
5
96% Ethanol
240
6
100% Ethano
Hey histonetters!
Keiser University is looking for a program coordinator for their
histotechnology program at their Pembroke Pines campus. For further
information please contact
Tania Phillips, MBA
Associate Dean - Allied Health
Keiser University - Pembroke Pines
12520 Pines Boulevard
Pe
Hello Histonetters,
I have been staining residues with Phloroglucinol which is light
sensitive however I due to time constraints I have not been able to wait
for all the colour to fade ( as this takes about one week). Therefore, I
have dried off the stained residue under lights and then countersta
The following info might be useful:
It is believed that formalin given time will kill any microorganisms
that are present in tissue and that formalin will inactivate
tuberculosis.
Vardaxis et al (J Clin Pathol 1997;50:429-433) were quite rightly
concerned with the disinfection of bacterial endosp
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