[Histonet] CD200(OX2) that works in FFPE mouse samples

2013-05-22 Thread Colleen Forster
To all the histonetters, IS anyone doing CD200 staining in FFPE mouse samples with good results? If so, please share your information. Thanks in advance...your help is always appreciated!! Colleen Forster U of MN ___ Histonet mailing list Histo

Re: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen

2013-05-22 Thread Krista Sider
Thank you all very much for you help. I really appreciate it. Gudrun: I tried the PTA before the WSAF and it worked beautifully (1 min PTA, dH2O, 5 min WSAF, dH20, AA, 10 min PTA). Red muscle and clean collagen.Thank-you Liz: I didn't get to trying the 60*C Bouin's but I will make a note for next

[Histonet] CHARLESTON, SC HISTOTECHS-I need a PRN HT

2013-05-22 Thread Wanda.Smith
I have a PRN Ht position available for early morning fill-ins and/or set days during the week. Upcoming maternity leave also. 4:00 am until you have to go to your other job or work part time for us. GREAT crew and Pathologist to work for and opportunities to multitask, i.e., embedding, cuttin

[Histonet] Looking for mouse antibody of LTa for use in IHC

2013-05-22 Thread Erickson, Jamie E
Hello Histonetters, Does anyone know of a antibody to mouse LTa (Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA)) that works in IHC. A colleague is pulling her hair out trying various clones in formalin and frozen sections of mouse spleen but with no luck, please help her.. Is there one th

RE: [Histonet] Oven recommendations

2013-05-22 Thread MaryK Mendell
Minnie This is what I have used for years as a dryer. You can go onto the TBS website : SD-II-120 Slide Dryer II, forced air, holds 2 conventional slide stainer racks, overheat protection switch, ambient to 75°C. Specifications: 100-120VAC, 50/60Hz, 2A; SD-II-220: 220-240VAC, 50/60Hz, 2A. Wx

[Histonet] Miraca Life Sciences Position's in KY

2013-05-22 Thread Hale, Meredith
Great opportunity for Histotechnician's in Crestview Hills, KY! Miraca Life Sciences is looking to fill 2 Full Time Histologist positions in Northern Kentucky. The position will be with Miraca Life Sciences and will include benefits. The candidates must meet and have documentation to support the

RE: [Histonet] Safranin O cartilage staining

2013-05-22 Thread Elizabeth Chlipala
Brett Safranin O can be a bit tricky at times. There are a few tips that we have used through the years. 1. Fresh reagents are key. 2. For proteoglycan staining you can cut the sections a bit thicker and get better staining we typically cut the joint sections for Saf O at 6 to 7 microns in

RE: [Histonet] Oven recommendations

2013-05-22 Thread WILLIAM DESALVO
Since you state you are in reasearch, consider air drying your slides. You only need to remove the water between the paraffin section and the glass slide to allow adhesion of the proteins in the tissue sample to the glass. Melting the paraffin in not necessary, your deparaffinization steps in th

[Histonet] Oven recommendations

2013-05-22 Thread Maribel Santiago
Hi All, I had just received a letter from Boekel (models 107800-107801 or 107905 in either 120v or 230v) that my oven can not be used to melt wax of any kind. as this is our bread and butter and need some suggestions to what kind or brand is used that is good for us (histologists). I need a

[Histonet] Looking for a Leica Bond or Dako Autostainer

2013-05-22 Thread Jill Cox
Hi Netters, We are looking for a used Leica Bond or Dako Autostainer. If anyone out there is upgrading or has one for sale please respond to this email..  Thank you, Jill ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwes

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

2013-05-22 Thread Boyd, Debbie M
We have two Excelsiors (had Three). We use Americlear in the cleaning cycle. We have never had issues with our cleaning cycle or the operation of our processors. Our oldest processor was 12 years old before the oven cracked and started leaking. The repair was too expensive for an older proce

Re: [Histonet] Unsubscribe

2013-05-22 Thread 木村裕樹
2013/05/22 12:26 "Lee & Peggy Wenk" : For everyone needing to unsubscribe over the summer months while on vacation, or just because . . . keep this email handy. Go to the bottom of any email. The last line is an internet address - starts with http://has the word mailman in it. Click on the