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Nancy Schmitt
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/05/2009 11:07 AM
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Subject
[Histonet] time in paraffin and fr
er, a
practice I do not think is adequate.
To your second question, just place them in NBF and when fixed filter and wrap
them yourself while cassetting, do not wrap them before being fixed.
René J.
--- On Mon, 10/5/09, Nancy Schmitt wrote:
From: Nancy Schmitt
Subject: [Histonet] time in paraffi
Good Morning Histonetters-
First question: Textbook says "tissue should remain in paraffin the shortest
time necessary for good infiltration because exposure to prolonged heat causes
shrinkage and hardening". Can anyone define "exposure to prolonged heat"? Is
that an hour? Three hours? Sitt