Re: [Histonet] time in paraffin and fried bloody specimen

2009-10-05 Thread DKBoyd
Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net Nancy Schmitt Sent by: histonet-boun...@lists.utsouthwestern.edu 10/05/2009 11:07 AM To "Histonet (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] time in paraffin and fr

Re: [Histonet] time in paraffin and fried bloody specimen

2009-10-05 Thread Rene J Buesa
er, a practice I do not think is adequate. To your second question, just place them in NBF and when fixed filter and wrap them yourself while cassetting, do not wrap them before being fixed. René J. --- On Mon, 10/5/09, Nancy Schmitt wrote: From: Nancy Schmitt Subject: [Histonet] time in paraffi

[Histonet] time in paraffin and fried bloody specimen

2009-10-05 Thread Nancy Schmitt
Good Morning Histonetters- First question: Textbook says "tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening". Can anyone define "exposure to prolonged heat"? Is that an hour? Three hours? Sitt