[Histonet] Maria added you as a friend on Reunion.com!

[Maria Katleba]

Hi,

I looked for you on Reunion.com, but you weren't there. Please 
connect with me so we can keep in touch.
-Maria

Do You Know Maria?
YES - Connect with Maria, and see who's searching for you

http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&[EMAIL
 
PROTECTED]&userid=646706&extra=&&&2002&&&http://www.reunion.com/showInviteRegistration.do?uid=297786637
NO - I don't know Maria 
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PROTECTED]&userid=646706&extra=&&&2000&&&http://www.reunion.com/showInviteRegistration.do?unsub=true&[EMAIL
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[Histonet] Maria added you as a friend on Reunion.com!

[Maria Katleba]

Hi,

I looked for you on Reunion.com, but you weren't there. Please 
connect with me so we can keep in touch.
-Maria

Do You Know Maria?
YES - Connect with Maria, and see who's searching for you

http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&[EMAIL
 
PROTECTED]&userid=646707&extra=&&&2002&&&http://www.reunion.com/showInviteRegistration.do?uid=297786637
NO - I don't know Maria 
http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&[EMAIL
 
PROTECTED]&userid=646707&extra=&&&2000&&&http://www.reunion.com/showInviteRegistration.do?unsub=true&[EMAIL
 PROTECTED]&uid=297786637


Reunion.com - Find Everyone from Your Past. 
You have received this e-mail because a Reunion.com Member sent 
an invitation to
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Contact Us. 
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Re: [Histonet] Re: Fungal stains & political views

[Akemi Allison-Tacha]

Hi All,

I totally agree with Robert on his information regarding periodic acid and 
chromic acid. I have done extensive research on this subject when I developed 
my special stain kits for Biocare Medical.  I did incorporate 10% chromic acid 
for the GMS stain, and 1% periodic acid for the PAS stain. 

As far as this forum for political views,  in frustration, I deleted everything 
with these headings.  There could of been some very interesting feed-back, but 
you have used this source totally inappropriately!  I believe in this countries 
beliefs for freedom of speech, but use it wisely!!  The histonet is NOT THE 
PLACE! 

Akemi Allison-Tacha, BS, HT(ASCP)HTL
Client Services Manager
PhenoPath laboratories
551 North 34th Street, Suite 100 
Seattle, WA 98103-8675
Work: (206) 374-9000 ext 1053
E-Mail: [EMAIL PROTECTED]


--- On Wed, 11/5/08, Robert Richmond <[EMAIL PROTECTED]> wrote:

> From: Robert Richmond <[EMAIL PROTECTED]>
> Subject: [Histonet] Re: Fungal stains
> To: histonet@lists.utsouthwestern.edu
> Date: Wednesday, November 5, 2008, 7:29 PM
> Colin Weaver asks about staining of various fungal species
> by the
> standard fungal stains.
> 
> The standard fungal stains have two basic steps:
> 
> 1. Periodic acid or chromic acid splits bonds between
> vicinal diol
> groups (two carbons with OH"s on adjacent carbon
> atoms) and oxidizes
> the free ends to aldehyde groups (-CHO).
> 
> 2. The aldehyde groups oxidize Schiff's reagent or
> methenamine silver
> to produce a visible deposit.
> 
> Periodic acid is not a sufficiently strong oxidant to
> demonstrate some
> fungal cell walls. In human pathology, Histoplasma is most
> likely to
> stain weakly or not at all. You need chromic acid, most
> usually
> followed by methenamine silver.
> 
> Unfortunately, many so-called GMS kits silently substitute
> periodic
> acid for the more hazardous chromic acid. Freida Carson
> reviewed this
> top in J Histotechnol several years ago - I can find the
> reference if
> anyone needs it (I think I've posted it on Histonet
> before, so it's
> probably in the archives.) So your first step is to see if
> you're
> actually using chromic acid.
> 
> PLEASE get the politics off this list - the repetitious
> garbage is
> making the list hard to read, particularly in digest form.
> Linda
> Margraf, if it doesn't stop, I suggest moderating the
> list for a few
> days, though not permanently.
> 
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
> 
> ___
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[Histonet] Re: Fungal stains

[Robert Richmond]

Colin Weaver asks about staining of various fungal species by the
standard fungal stains.

The standard fungal stains have two basic steps:

1. Periodic acid or chromic acid splits bonds between vicinal diol
groups (two carbons with OH"s on adjacent carbon atoms) and oxidizes
the free ends to aldehyde groups (-CHO).

2. The aldehyde groups oxidize Schiff's reagent or methenamine silver
to produce a visible deposit.

Periodic acid is not a sufficiently strong oxidant to demonstrate some
fungal cell walls. In human pathology, Histoplasma is most likely to
stain weakly or not at all. You need chromic acid, most usually
followed by methenamine silver.

Unfortunately, many so-called GMS kits silently substitute periodic
acid for the more hazardous chromic acid. Freida Carson reviewed this
top in J Histotechnol several years ago - I can find the reference if
anyone needs it (I think I've posted it on Histonet before, so it's
probably in the archives.) So your first step is to see if you're
actually using chromic acid.

PLEASE get the politics off this list - the repetitious garbage is
making the list hard to read, particularly in digest form. Linda
Margraf, if it doesn't stop, I suggest moderating the list for a few
days, though not permanently.

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] Re: bone marrow biopsies

[Ingles Claire]

Hey gang - 
I have taken a bit of a proactive approach to this spamming and contacted 
google e-mail  (where 'Sam' sends little notes from) about our little problem. 
I don't know if it will do any good, but I did remind them that this activity 
IS against their use policy. Hopefully we won't be hearing from 'Sam' any more 
after this.
Claire



From: [EMAIL PROTECTED] on behalf of Sam Histology
Sent: Wed 11/5/2008 3:08 PM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: bone marrow biopsies



At home, there will be an increase in taxes-income, estate, payroll-to
fund more government health care, education, and general entitlement
programs. The old Reaganesque notion that government subsidies can
make one more dependent, angrier, and envious is forgotten, along with
the notion that lower taxes stimulate economic growth and encourage
risk-taking, innovation, and independence. I worry especially about
the lifting of income caps (how far?) on social security taxes
inasmuch as they were part of the original covenant justifying the
caps on benefits paid out.

NAFTA and other free trade agreements would be repealed; illegal
immigration would either not be an issue, or more a problem of finding
the right way, with borders still open, to grant amnesties.
Appointments would hinge on a belief in bigger government and the
theme that the individual is currently suffering due to reactionaries
in government and corporations, barely housed, fed, or educated, and
deserves more federal dollars appropriated from others who either
don't need all their income or didn't deserve the compensation they
were given.

Abroad, there is a general argument that things are going terribly.
Forget that the Taliban and Saddam are gone. Forget that we have not
suffered another 9/11 attack. Forget that there is far more democratic
promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in
2001. Forget that the Merkel and Sarkozy governments, along with
Eastern European leaders, are more pro-American than their
predecessors in 2001.

Instead, we are disliked by everyone, and for good reasons. The fact
that Iranian mullahs, the House of Saud cousins, Hugo Chavez's
communists, European mullahs, and the Arab street don't approve of
America says more about us than it does them. The solution is to
follow more the dictates of European Union and United Nations, where
sophisticated internationalists can guide us through the maze of
global power, instructing mostly ignorant Americans how and why we
tend to cause so many of the world's problems. Misunderstanding and
our own obtuseness explain global tension, not the agendas of enemies
who know exactly what they want and how to get it.

Our military is not so much an offensive force, designed to defeat and
kill our enemies, that needs support and constant honing; better to
see it as a large social organization that we must look at in terms
only of proper rotations, health care, and benefits. We are to support
the troops not in the sense of doing everything we can to ensure they
win, and gain the proper recognition for their courage and sacrifice,
but rather in consideration of their victimhood, offering proper
sympathy and remediation for the defeat in Iraq, the unwise use of
their skills, and the needless loss of their lives.

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Re: [Histonet] ASCP HT maintenance fees

[Nicholas Rosenbaum]

Amen, Amos! Amen!


Nicholas Rosenbaum HT, (ASCP)



From: Amos Brooks <[EMAIL PROTECTED]>
To: "histonet@lists.utsouthwestern.edu" 
Sent: Tuesday, November 4, 2008 7:39:51 PM
Subject: [Histonet] ASCP HT maintenance fees

Hi,
   I don't mean to be the fly in the ointment here, but in the words of
Janet Jackson "What have you done for me lately?". It's a long standing joke
among sticker collectors that aside from the certification the sticker is
all you really get from the society.
   Where was the ASCP when NYS decided to pass that whacky law causing
histotechs basically to have to be medtechs? The legislation seemed to
actually take even them by surprise. There was little warning that this was
going to happen. The main school for histology (SUNY Cobleskill) actually
closed for a while after this.
   The proper thing for an advocate for histotechnology woule be to inform
the profession of upcoming changes. Help those affected, and certainly make
sure the educational requirements are fully lined up prior to the final
passage of the laws. That is if they were paying attention.
   Where was ASCP? Worrying about medtechs perhaps. To my eye ASCP has much
less interest in histotechnology (after certification) than histotechnology
has in the ASCP.

Just an observation,
Amos



Message: 12
Date: Tue, 4 Nov 2008 08:23:02 -0700
From: "Patsy Ruegg" <[EMAIL PROTECTED]>
Subject: RE: [Histonet] ASCP HT maintenance fees
To: "'R C'" <[EMAIL PROTECTED]>,  "'Podawiltz, Thomas'"
   <[EMAIL PROTECTED]>
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
   <[EMAIL PROTECTED]>
Content-Type: text/plain;   charset="us-ascii"
One of the benefits of ASCP membership is to support their efforts to fight
for the medical technology profession in local and national legislation.
With shortages, licensure, billing and all sorts of political issues that
affect our lives we need someone to be working for us.  CAP and CLIA does
not do much for HT's since they still do not even require that a certified
HT be doing the job. I get reports almost daily on what ASCP is doing for us
and I support it by paying my membership dues.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
[EMAIL PROTECTED]
www.ihctech.net
www.ihcrg.org
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Re: [Histonet] stain

[Bryan Llewellyn]

Altmann's acid fuchsin picric acid is a histological stain for mitochondria.

It requres the tissue to be fixed in a non-acid dichromate fixative, then 
post-chromed for some time.  The tissue is then processed by paraffin and 
sectioned.


Staining is in aniline water saturated with acid fuchsin, which is applied 
much like carbol fuchsin in a ZN, i.e. heated.  Diffeerentiation is with 
picric acid.


The method requires considerable skill from the technologist, but produces 
red mitochondria on a yellow background.


I suggest that if you just want to demonstrate mitochondria, do a succinate 
dehydrogenase instead, it is much easier and much faster.


If you want, I can look up the details and send them on, but I recall they 
used to be in Culling's book.


Bryan Llewellyn


- Original Message - 
From: "Patsy Ruegg" <[EMAIL PROTECTED]>

To: <[EMAIL PROTECTED]>
Sent: Wednesday, November 05, 2008 2:27 PM
Subject: [Histonet] stain



Anyone familiar with this stain?



Altmann acid-fushsin picric. This stain is a histochemical stain for
mitochondria in intestine.



Best regards,



Patsy





Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email [EMAIL PROTECTED]
web site www.ihctech.net




This email is confidential and intended solely for the use of the 
Person(s)

('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that 
is
privileged & confidential within the meaning of applicable law. 
Accordingly

any dissemination, distribution, copying, or other use of this message, or
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Re: [Histonet] 200 proof alcohol?

[Joe Nocito]

200 proof is 200 proof, can't get any purer than that. Of course, I would 
take the taste test first. Green olives stuffed with Feta cheese goes good.


JTT
- Original Message - 
From: "Caroline Bass" <[EMAIL PROTECTED]>

To: 
Sent: Wednesday, November 05, 2008 11:15 AM
Subject: [Histonet] 200 proof alcohol?


Hello,

I¹m trying to buy some alcohol for both histology and molecular biology
protocols. But I¹m having some difficult getting ³molecular biology grade²
100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say
anything about the quality. Since it¹s 200 proof, is that basically the same
as molecular biology grade? How about histology grade? I seem to recall
using hospital provided ethanol for molecular biology experiments in my
postdoc, so I think it should be ok.

Any advice is appreciated!

Caroline
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RE: [Histonet] Re: bone marrow biopsies

[Tony Henwood]

Only if he is well fixed first!!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Robyn
Vazquez
Sent: Thursday, 6 November 2008 8:25 AM
To: Sam Histology; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: bone marrow biopsies


Can you block this person?

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Sam
Histology
Sent: Wednesday, November 05, 2008 1:09 PM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: bone marrow biopsies

At home, there will be an increase in taxes-income, estate, payroll-to
fund more government health care, education, and general entitlement
programs. The old Reaganesque notion that government subsidies can make
one more dependent, angrier, and envious is forgotten, along with the
notion that lower taxes stimulate economic growth and encourage
risk-taking, innovation, and independence. I worry especially about the
lifting of income caps (how far?) on social security taxes inasmuch as
they were part of the original covenant justifying the caps on benefits
paid out.

NAFTA and other free trade agreements would be repealed; illegal
immigration would either not be an issue, or more a problem of finding
the right way, with borders still open, to grant amnesties. Appointments
would hinge on a belief in bigger government and the theme that the
individual is currently suffering due to reactionaries in government and
corporations, barely housed, fed, or educated, and deserves more federal
dollars appropriated from others who either don't need all their income
or didn't deserve the compensation they were given.

Abroad, there is a general argument that things are going terribly.
Forget that the Taliban and Saddam are gone. Forget that we have not
suffered another 9/11 attack. Forget that there is far more democratic
promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in
2001. Forget that the Merkel and Sarkozy governments, along with Eastern
European leaders, are more pro-American than their predecessors in 2001.

Instead, we are disliked by everyone, and for good reasons. The fact
that Iranian mullahs, the House of Saud cousins, Hugo Chavez's
communists, European mullahs, and the Arab street don't approve of
America says more about us than it does them. The solution is to follow
more the dictates of European Union and United Nations, where
sophisticated internationalists can guide us through the maze of global
power, instructing mostly ignorant Americans how and why we tend to
cause so many of the world's problems. Misunderstanding and our own
obtuseness explain global tension, not the agendas of enemies who know
exactly what they want and how to get it.

Our military is not so much an offensive force, designed to defeat and
kill our enemies, that needs support and constant honing; better to see
it as a large social organization that we must look at in terms only of
proper rotations, health care, and benefits. We are to support the
troops not in the sense of doing everything we can to ensure they win,
and gain the proper recognition for their courage and sacrifice, but
rather in consideration of their victimhood, offering proper sympathy
and remediation for the defeat in Iraq, the unwise use of their skills,
and the needless loss of their lives.

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Views expressed in this message and any attachments are those of the individual 
sender, and are not necessarily the views of The Children's Hospital at Westmead

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RE: [Histonet] Fatty tissue

[Tony Henwood]

I would suggest microwaving the tissue blocks in formalin for at least 1
hour (longer is better) up to a temperature of 45oC (you might even
consider a higher temp maybe up to 55oC??)

We use this with our placenta blocks and the results were dramatically
improved (35% poorly processed to less than 1% after microwaving).

We have a Mega TT Microwave oven 

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Webb,
Dorothy L
Sent: Thursday, 6 November 2008 7:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fatty tissue


I would like to know how other labs are processing "fatty" tissue,
especially breast for optimal processing?  We have been doing a few
"reprocessing" of these specimens and my pathologist feels this should
not be happening!  Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick, but, at times the tumor is ingrained in the
fatty area and they need to obtain as much of the perimeter as they can.
Does anyone agitate or heat fixate their specimens prior to processing?
Would appreciate any feedback on this age old dilemma. Also, I only
heard back from one person on the recycling issue.  Any input would be
appreciated as to savings incurred and a still vs. recycler.

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[Histonet] stain

[Patsy Ruegg]

Anyone familiar with this stain?

 

Altmann acid-fushsin picric. This stain is a histochemical stain for
mitochondria in intestine.

 

Best regards,

 

Patsy

 

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email [EMAIL PROTECTED]
web site www.ihctech.net

 


This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

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Re: [Histonet] Re: bone marrow biopsies

[Larry Woody]

Even better we will find where he is.
 
Larry A. Woody
Seattle, Wa.





From: Robyn Vazquez <[EMAIL PROTECTED]>
To: Sam Histology <[EMAIL PROTECTED]>; Histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 5, 2008 1:24:58 PM
Subject: RE: [Histonet] Re: bone marrow biopsies

Can you block this person?

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sam Histology
Sent: Wednesday, November 05, 2008 1:09 PM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: bone marrow biopsies

At home, there will be an increase in taxes—income, estate, payroll—to
fund more government health care, education, and general entitlement
programs. The old Reaganesque notion that government subsidies can
make one more dependent, angrier, and envious is forgotten, along with
the notion that lower taxes stimulate economic growth and encourage
risk-taking, innovation, and independence. I worry especially about
the lifting of income caps (how far?) on social security taxes
inasmuch as they were part of the original covenant justifying the
caps on benefits paid out.

NAFTA and other free trade agreements would be repealed; illegal
immigration would either not be an issue, or more a problem of finding
the right way, with borders still open, to grant amnesties.
Appointments would hinge on a belief in bigger government and the
theme that the individual is currently suffering due to reactionaries
in government and corporations, barely housed, fed, or educated, and
deserves more federal dollars appropriated from others who either
don't need all their income or didn't deserve the compensation they
were given.

Abroad, there is a general argument that things are going terribly.
Forget that the Taliban and Saddam are gone. Forget that we have not
suffered another 9/11 attack. Forget that there is far more democratic
promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in
2001. Forget that the Merkel and Sarkozy governments, along with
Eastern European leaders, are more pro-American than their
predecessors in 2001.

Instead, we are disliked by everyone, and for good reasons. The fact
that Iranian mullahs, the House of Saud cousins, Hugo Chavez's
communists, European mullahs, and the Arab street don't approve of
America says more about us than it does them. The solution is to
follow more the dictates of European Union and United Nations, where
sophisticated internationalists can guide us through the maze of
global power, instructing mostly ignorant Americans how and why we
tend to cause so many of the world's problems. Misunderstanding and
our own obtuseness explain global tension, not the agendas of enemies
who know exactly what they want and how to get it.

Our military is not so much an offensive force, designed to defeat and
kill our enemies, that needs support and constant honing; better to
see it as a large social organization that we must look at in terms
only of proper rotations, health care, and benefits. We are to support
the troops not in the sense of doing everything we can to ensure they
win, and gain the proper recognition for their courage and sacrifice,
but rather in consideration of their victimhood, offering proper
sympathy and remediation for the defeat in Iraq, the unwise use of
their skills, and the needless loss of their lives.

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RE: [Histonet] Re: bone marrow biopsies

[Robyn Vazquez]

Can you block this person?

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sam Histology
Sent: Wednesday, November 05, 2008 1:09 PM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: bone marrow biopsies

At home, there will be an increase in taxes—income, estate, payroll—to
fund more government health care, education, and general entitlement
programs. The old Reaganesque notion that government subsidies can
make one more dependent, angrier, and envious is forgotten, along with
the notion that lower taxes stimulate economic growth and encourage
risk-taking, innovation, and independence. I worry especially about
the lifting of income caps (how far?) on social security taxes
inasmuch as they were part of the original covenant justifying the
caps on benefits paid out.

NAFTA and other free trade agreements would be repealed; illegal
immigration would either not be an issue, or more a problem of finding
the right way, with borders still open, to grant amnesties.
Appointments would hinge on a belief in bigger government and the
theme that the individual is currently suffering due to reactionaries
in government and corporations, barely housed, fed, or educated, and
deserves more federal dollars appropriated from others who either
don't need all their income or didn't deserve the compensation they
were given.

Abroad, there is a general argument that things are going terribly.
Forget that the Taliban and Saddam are gone. Forget that we have not
suffered another 9/11 attack. Forget that there is far more democratic
promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in
2001. Forget that the Merkel and Sarkozy governments, along with
Eastern European leaders, are more pro-American than their
predecessors in 2001.

Instead, we are disliked by everyone, and for good reasons. The fact
that Iranian mullahs, the House of Saud cousins, Hugo Chavez's
communists, European mullahs, and the Arab street don't approve of
America says more about us than it does them. The solution is to
follow more the dictates of European Union and United Nations, where
sophisticated internationalists can guide us through the maze of
global power, instructing mostly ignorant Americans how and why we
tend to cause so many of the world's problems. Misunderstanding and
our own obtuseness explain global tension, not the agendas of enemies
who know exactly what they want and how to get it.

Our military is not so much an offensive force, designed to defeat and
kill our enemies, that needs support and constant honing; better to
see it as a large social organization that we must look at in terms
only of proper rotations, health care, and benefits. We are to support
the troops not in the sense of doing everything we can to ensure they
win, and gain the proper recognition for their courage and sacrifice,
but rather in consideration of their victimhood, offering proper
sympathy and remediation for the defeat in Iraq, the unwise use of
their skills, and the needless loss of their lives.

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Re: [Histonet] re: Biocare Decloaker HIER

[Piero Nelva]

Ooops.  One too many ones!

Piero

- Original Message - 
From: "Linda M Watson" <[EMAIL PROTECTED]>

To: "Piero Nelva" <[EMAIL PROTECTED]>
Cc: "histonet" 
Sent: Wednesday, November 05, 2008 11:07 PM
Subject: Re: [Histonet] re: Biocare Decloaker HIER



Is the 118 psi a typo? I don't believe the instrument can go that
high-max psi is 30.

Piero Nelva wrote:


I used it for 4 minutes at about 118 psi and most antibodies worked
really well.  I was having huge problems with the Er and Her2 and this
solved them (almost) overnight.


- Original Message - From: "Jan Shivers" <[EMAIL PROTECTED]>
To: "histonet" 
Sent: Wednesday, November 05, 2008 3:12 AM
Subject: [Histonet] re: Biocare Decloaker HIER


For those of you who use Biocare's Decloaker for HIER on regular cases
(not prion protein retrieval), could you tell me how long you do HIER
with these machines?  I normally do my prion cases in a Decloaker for
20', with a 25' cooldown, but I suspect that regular tissues don't
need that length of time at high pressure/temp.  I have an extra
Decloaker and am considering switching to this device for my normal
case work HIER, if it's time efficient.

Please reply privately.   Thanks.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
[EMAIL PROTECTED]
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Checked by AVG - http://www.avg.com
Version: 8.0.175 / Virus Database: 270.8.6/1765 - Release Date:
11/3/2008 4:59 PM


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9:38 PM



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Re: [Histonet] Re: bone marrow biopsies

[Sam Histology]

At home, there will be an increase in taxes—income, estate, payroll—to
fund more government health care, education, and general entitlement
programs. The old Reaganesque notion that government subsidies can
make one more dependent, angrier, and envious is forgotten, along with
the notion that lower taxes stimulate economic growth and encourage
risk-taking, innovation, and independence. I worry especially about
the lifting of income caps (how far?) on social security taxes
inasmuch as they were part of the original covenant justifying the
caps on benefits paid out.

NAFTA and other free trade agreements would be repealed; illegal
immigration would either not be an issue, or more a problem of finding
the right way, with borders still open, to grant amnesties.
Appointments would hinge on a belief in bigger government and the
theme that the individual is currently suffering due to reactionaries
in government and corporations, barely housed, fed, or educated, and
deserves more federal dollars appropriated from others who either
don't need all their income or didn't deserve the compensation they
were given.

Abroad, there is a general argument that things are going terribly.
Forget that the Taliban and Saddam are gone. Forget that we have not
suffered another 9/11 attack. Forget that there is far more democratic
promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in
2001. Forget that the Merkel and Sarkozy governments, along with
Eastern European leaders, are more pro-American than their
predecessors in 2001.

Instead, we are disliked by everyone, and for good reasons. The fact
that Iranian mullahs, the House of Saud cousins, Hugo Chavez's
communists, European mullahs, and the Arab street don't approve of
America says more about us than it does them. The solution is to
follow more the dictates of European Union and United Nations, where
sophisticated internationalists can guide us through the maze of
global power, instructing mostly ignorant Americans how and why we
tend to cause so many of the world's problems. Misunderstanding and
our own obtuseness explain global tension, not the agendas of enemies
who know exactly what they want and how to get it.

Our military is not so much an offensive force, designed to defeat and
kill our enemies, that needs support and constant honing; better to
see it as a large social organization that we must look at in terms
only of proper rotations, health care, and benefits. We are to support
the troops not in the sense of doing everything we can to ensure they
win, and gain the proper recognition for their courage and sacrifice,
but rather in consideration of their victimhood, offering proper
sympathy and remediation for the defeat in Iraq, the unwise use of
their skills, and the needless loss of their lives.

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Re: [Histonet] Fatty tissue

[Rene J Buesa]

Dorothy:
"Reprocessing" fatty tissue (usually a hopeless proposition) is essentially 
caused by poor infiltration more than by poor fixation, and poor infiltration 
is usually caused by less than optimal time in the clearing agent or antemedium 
(usually xylene).
The thing is that fatty tissue contains less water than other tissues and will 
dehydrate excessively in the routine protocol, but requires more clearing time 
than in the "normal" protocols.
The solution is to shorten the dehydration period and increase the clearing 
times or, as I solved the problem, by eliminating xylene altogether when I 
started using a clearing mixture composed of isopropyl alcohol and mineral oil.
René J.

--- On Wed, 11/5/08, Webb, Dorothy L <[EMAIL PROTECTED]> wrote:

From: Webb, Dorothy L <[EMAIL PROTECTED]>
Subject: [Histonet] Fatty tissue
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, November 5, 2008, 3:21 PM

I would like to know how other labs are processing "fatty" tissue,
especially breast for optimal processing?  We have been doing a few
"reprocessing" of these specimens and my pathologist feels this
should
not be happening!  Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick, but, at times the tumor is ingrained in the
fatty area and they need to obtain as much of the perimeter as they can.
Does anyone agitate or heat fixate their specimens prior to processing?
Would appreciate any feedback on this age old dilemma.
Also, I only heard back from one person on the recycling issue.  Any
input would be appreciated as to savings incurred and a still vs.
recycler.

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[Histonet] Male and female fungal organisms? Chromic and periodic acid

[Madary, Joseph]

Gridley Fungus is a stain that uses chromic as the oxidizer and schiffs
reagent but finishes off with aldehyde fuchsin(and maybe Metanil
Yellow?), and provides a light stain, but great for morphology.  I do
not know how specific it is but when I was at AFIP in the Infectious
Disease Lab, we used it a lot in fungal panels.

What did the female mushroom say to the male mushroom?  Hey you are a
real fun guy(get it fun gi?).  I made a funny.

Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory Mgr
One Medimmune Way, Lab 2438-Area 4
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose. Thank you for your cooperation.





To the extent this electronic communication or any of its attachments contain 
information that is not in the public domain, such information is considered by 
MedImmune to be confidential and proprietary.  This communication is expected 
to be read and/or used only by the individual(s) for whom it is intended.  If 
you have received this electronic communication in error, please reply to the 
sender advising of the error in transmission and delete the original message 
and any accompanying documents from your system immediately, without copying, 
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[Histonet] Fatty tissue

[Webb, Dorothy L]

I would like to know how other labs are processing "fatty" tissue,
especially breast for optimal processing?  We have been doing a few
"reprocessing" of these specimens and my pathologist feels this should
not be happening!  Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick, but, at times the tumor is ingrained in the
fatty area and they need to obtain as much of the perimeter as they can.
Does anyone agitate or heat fixate their specimens prior to processing?
Would appreciate any feedback on this age old dilemma.
Also, I only heard back from one person on the recycling issue.  Any
input would be appreciated as to savings incurred and a still vs.
recycler.

This e-mail and any files transmitted with it are confidential and are intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient or the individual responsible for delivering 
the e-mail to the intended recipient, please be advised that you have received 
this e-mail in error and that any use, dissemination, forwarding, printing, or 
copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the 
HealthPartners Support Center by telephone at (952) 967-6600. You will be 
reimbursed for reasonable costs incurred in notifying us.
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Re: [Histonet] 200 proof alcohol?

[John C. Dennis]

Caroline

I would not recommend using the same alcohol for histo that you use for 
molecular work.  Assuming by "molecular" you mean isolating DNA/RNA, 
cloning, etc.  You'll want a higher grade, more expensive alcohol for 
nucleic acid work then the histological stuff that comes in a 55 gal drum.


John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Wed, 5 Nov 2008, Caroline Bass wrote:


Hello,

I¹m trying to buy some alcohol for both histology and molecular biology
protocols. But I¹m having some difficult getting ³molecular biology grade²
100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say
anything about the quality. Since it¹s 200 proof, is that basically the same
as molecular biology grade? How about histology grade? I seem to recall
using hospital provided ethanol for molecular biology experiments in my
postdoc, so I think it should be ok.

Any advice is appreciated!

Caroline
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RE: [Histonet] funding for NIH

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

unfortunately, all government funding comes from us peons...that's
the way it has always been and will always be. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
[EMAIL PROTECTED]


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Peter
Carroll
Sent: Wednesday, November 05, 2008 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] funding for NIH


 > because that would mean more taxes!

Making more than $250,000/year doing histology? Where do I sign up?! ;)


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[Histonet] Cytology QA Plan

[Rutledge, Nancy]

We just started doing NONGYN cytology.  We have no cytologist.  Does
anyone have a suggestion and/or a starting place for how to develop a QA
Plan.  

 

Thanks in advance.

 

Nancy 

 

 

~~
This email and any files transmitted with it are confidential, and intended 
solely for the use of the individual or entity to whom they are addressed. If 
you have received this email in error  contact the Help Desk for Cape Cod 
Healthcare.

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Re: [Histonet] funding for NIH

[Peter Carroll]

> because that would mean more taxes!

Making more than $250,000/year doing histology? Where do I sign up?! ;)


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RE: [Histonet] Re: bone marrow biopsies

[Weems, Joyce]

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[Histonet] Fwd: Texas Society for Histotechnology 2009 Call for Abstracts

[kdwyer3322]

-Original Message-
From: [EMAIL PROTECTED]
To: histonet@lists.utsouthwestern.edu
Sent: Sun, 2 Nov 2008 7:04 pm
Subject: Texas Society for Histotechnology 2009 Call for Abstracts



Histonetters, 

The Texas Society for Histotechnology will be holding it 2009 meeting in 
beautiful Austin Texas on May 28-31, 2009 (This is not Memorial weekend) at the 
Hyatt Regency Austin Lady Bird Lake, 208 Barton Springs, Austin, Texas.? 

The program committee is actively seeking workshop/symposum abstracts for this 
meeting.? If you or anyone you know would like to submit a workshop to the 
meeting please contact: 

Kathy Dwyer?- [EMAIL PROTECTED] ?
OR
Veronica Davis - [EMAIL PROTECTED]

Attached is the TSH 2009 Call for Abstracts. 

Thanks, 
TSH Convention Committe


McCain or Obama? Stay up to date on the latest from the campaign trail with AOL 
News. 

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[Histonet] Texas Society for Histotechnology 2009 Call for Abstracts

[kdwyer3322]

Histonetters, 

The Texas Society for Histotechnology will be holding it 2009 meeting in 
beautiful Austin Texas on May 28-31, 2009 (This is not Memorial weekend) at the 
Hyatt Regency Austin Lady Bird Lake, 208 Barton Springs, Austin, Texas.? 

The program committee is actively seeking workshop/symposum abstracts for this 
meeting.? If you or anyone you know would like to submit a workshop to the 
meeting please contact: 

Kathy Dwyer?- [EMAIL PROTECTED] ?
OR
Veronica Davis - [EMAIL PROTECTED]

Attached is the TSH 2009 Call for Abstracts. 

Thanks, 
TSH Convention Committe
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[Histonet] 200 proof alcohol?

[Caroline Bass]

Hello,

I¹m trying to buy some alcohol for both histology and molecular biology
protocols. But I¹m having some difficult getting ³molecular biology grade²
100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say
anything about the quality. Since it¹s 200 proof, is that basically the same
as molecular biology grade? How about histology grade? I seem to recall
using hospital provided ethanol for molecular biology experiments in my
postdoc, so I think it should be ok.

Any advice is appreciated!

Caroline
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[Histonet] president

[Hehl Joachim]

Please receive my apology. It was a provocation 


-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED] im Auftrag von [EMAIL PROTECTED]
Gesendet: Mi 05.11.2008 19:09
An: histonet@lists.utsouthwestern.edu
Betreff: Histonet Digest, Vol 60, Issue 9
 
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. prostate core processing (karen adams)
   2. Thank you! ([EMAIL PROTECTED])
   3. President (Joachim Hehl)
   4. Gina Rodriguez is out of the office.
  ([EMAIL PROTECTED])
   5. What the heck is this doiing on the HistoNet???
  ([EMAIL PROTECTED])
   6. RE: What the heck is this doiing on the HistoNet???
  (Mickie Johnson)
   7. Fungal staining (Weaver, Colin)
   8. Re: funding for NIH (Mark Tarango)
   9. Re: Fungal staining (Larry Woody)
  10. AW: [Histonet] Fungal staining (Gudrun Lang)
  11. RE: funding for NIH (Bartlett, Jeanine (CDC/CCID/NCZVED))
  12. Political SPAM (Dawson, Glen)


--

Message: 1
Date: Wed, 5 Nov 2008 10:10:51 -0500
From: "karen adams" <[EMAIL PROTECTED]>
Subject: [Histonet] prostate core processing
To: histonet@lists.utsouthwestern.edu
Message-ID:
<[EMAIL PROTECTED]>
Content-Type: text/plain; charset=ISO-8859-1

Greetings,

 Can anyone offer a processing schedual for prostate bx cores for the
Leica processor??


--

Message: 2
Date: Wed, 5 Nov 2008 10:54:32 EST
From: [EMAIL PROTECTED]
Subject: [Histonet] Thank you!
To: histonet@lists.utsouthwestern.edu
Message-ID: <[EMAIL PROTECTED]>
Content-Type: text/plain; charset="US-ASCII"

Many thanks for intervening.
 
Elfi Hacker
Hacker Industries
Winnsboro, SC
 

Dear Histonetters:
I am the list administrator and I would like to  ask that people please 
refrain from further political comments on the list.  I know this is a touchy 
topic but this is not the forum for these  discussions.  Please remember, my 
colleagues and I at the University of  Texas Southwestern Medical Center run 
this list using University resources  and it would be a real shame if they 
were to decide this was not a good use  of their funds due to the off-topic 
comments etc. The Histonet list is an  amazing collection of >3300 people 
from around the world helping each  other and we really need to stick to 
topics pertinent to Histology.  Thanks.
Linda M
Histonet administrator

Linda Margraf,  MD
Professor of Pathology
University of Texas Southwestern Medical  School
Dallas TX USA
**AOL Search: Your one stop for directions, recipes and all other 
Holiday needs. Search Now. 
(http://pr.atwola.com/promoclk/10075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from
-aol-search/?ncid=emlcntussear0001)


--

Message: 3
Date: Wed, 05 Nov 2008 16:58:03 +0100
From: Joachim Hehl <[EMAIL PROTECTED]>
Subject: [Histonet] President
To: 
Message-ID: <[EMAIL PROTECTED]>
Content-Type: text/plain;   charset="US-ASCII"

Interesting, the histonet list seems to be occupied by ultraconservatives.




--

Message: 4
Date: Wed, 5 Nov 2008 10:00:57 -0600
From: [EMAIL PROTECTED]
Subject: [Histonet] Gina Rodriguez is out of the office.
To: histonet@lists.utsouthwestern.edu
Message-ID:
<[EMAIL PROTECTED]>

Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  11/05/2008 and will not return until
11/10/2008.

I will respond to your message when I return.


__
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
__



--

Message: 5
Date: Wed, 5 Nov 2008 10:52:25 EST
From: [EMAIL PROTECTED]
Subject: [Histonet] What the heck is this doiing on the HistoNet???
To: Histonet@lists.utsouthwestern.edu
Message-ID: <[EMAIL PROTECTED]>
Content-Type: text/plain; charset="US-ASCII"

This kind of material really should not be posted on the HistoNet! Why  don't 
you find some other place to vent!
 
Elfi Hacker
Hacker Industries Inc
 
http://www.youtube.com/watch?v=wFYk7a3vEyo

I don't know how many  times I've heard the words "Obama" and "Messiah" used
in the same sentence.  To liberals, Barack Obama is a spotless god-like
figure who will save America  from the scourge of neo-conservatism. I'm
totally sick of hearing and reading  your hero-w

Re: [Histonet] Gina Rodriguez is out of the office.

[Sam Histology]

> I will be out of the office starting  11/05/2008 and will not return until 
> 11/10/2008.

This kind of material really should not be posted on the HistoNet! Why
are you posting something that's off-topic like this? This is a
Histology list, not a 'I will be out of the office' list! They might
shut down the list!

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[Histonet] Political SPAM

[Dawson, Glen]

All,

Just an FYI: these political postings are SPAM.  These are not real histonet 
users, they are generic postings that are fired everywhere like viagra 
solicitations.  In other words, there is no real need to get upset with 
histonet users or administrators for random postings that everyone that has 
email or signs up for a listserv. will be getting by no choice or fault of 
their own.

Glen Dawson
Milwaukee, WI

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RE: [Histonet] funding for NIH

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

All I will say on the matter is that if more funding is approved I hope
it is simply pulled from somewhere else and is not in addition
to.because that would mean more taxes! 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
[EMAIL PROTECTED]


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Mark
Tarango
Sent: Wednesday, November 05, 2008 12:17 PM
To: Geoff McAuliffe
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] funding for NIH

I definately hope that now that Obama is going to be President that the
reseach will get moving again.  He's got The Congress too.

P.S.  I hope this message doesn't shut the histonet down.

Mark


On 11/5/08, Geoff McAuliffe <[EMAIL PROTECTED]> wrote:
>
> Hi Emily et al.
>
> The President, any President, can put $ for NIH is the budget request 
> he/she submits to Congress but ONLY Congress can appropriate money. 
> That is in the Constitution.
> Several of my colleagues are out of work due to loss of grant support 
> 'cause NIH, while not broke, has many worthy projects to fund and 
> cannot fund them all.
>
> Geoff
>
> Emily Sours wrote:
>
>> Isn't the election over?
>> Griping will do you no good, spambot!
>>
>> So, being in grant funded research, I hope our new president will put

>> some money back into NIH.
>> (I was hoping that no matter who won, so my political views will not 
>> spam
>> you)
>> Anyone else feel that way?
>>
>> Emily
>>
>>
>
>
> --
> --
> **
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583 [EMAIL PROTECTED]
> **
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Re: [Histonet] Fungal staining

[Larry Woody]

Maybe Aspergillus or Rhizopus.
 
Larry A. Woody
Seattle, Wa.





From: "Weaver, Colin" <[EMAIL PROTECTED]>
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 5, 2008 9:02:37 AM
Subject: [Histonet] Fungal staining

Hi - everyone - does anyone know of any fungi that can not be
demonstrated by PAS stain? We appear to have one in a cow placenta which
is obvious on Grocott and H&E but do not stain on PAS. The micro lab
isolated Absidia spp but the hyphae in the Grocott are septate but I
believe Absidia are aseptate. Any ideas would be most welcome.

Colin Weaver
VLA Thirsk
England



  

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Re: [Histonet] funding for NIH

[Mark Tarango]

I definately hope that now that Obama is going to be President that the
reseach will get moving again.  He's got The Congress too.

P.S.  I hope this message doesn't shut the histonet down.

Mark


On 11/5/08, Geoff McAuliffe <[EMAIL PROTECTED]> wrote:
>
> Hi Emily et al.
>
> The President, any President, can put $ for NIH is the budget request
> he/she submits to Congress but ONLY Congress can appropriate money. That is
> in the Constitution.
> Several of my colleagues are out of work due to loss of grant support
> 'cause NIH, while not broke, has many worthy projects to fund and cannot
> fund them all.
>
> Geoff
>
> Emily Sours wrote:
>
>> Isn't the election over?
>> Griping will do you no good, spambot!
>>
>> So, being in grant funded research, I hope our new president will put some
>> money back into NIH.
>> (I was hoping that no matter who won, so my political views will not spam
>> you)
>> Anyone else feel that way?
>>
>> Emily
>>
>>
>
>
> --
> --
> **
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583 [EMAIL PROTECTED]
> **
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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AW: [Histonet] Fungal staining

[Gudrun Lang]

Try a CAS stain. = PAS with Chromic acid 
Perhaps this fungus needs something stronger than Periodic acid.

Gudrun

-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Weaver,
Colin
Gesendet: Mittwoch, 05. November 2008 18:03
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Fungal staining

Hi - everyone - does anyone know of any fungi that can not be
demonstrated by PAS stain? We appear to have one in a cow placenta which
is obvious on Grocott and H&E but do not stain on PAS. The micro lab
isolated Absidia spp but the hyphae in the Grocott are septate but I
believe Absidia are aseptate. Any ideas would be most welcome.

Colin Weaver
VLA Thirsk
England



  

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[Histonet] Fungal staining

[Weaver, Colin]

Hi - everyone - does anyone know of any fungi that can not be
demonstrated by PAS stain? We appear to have one in a cow placenta which
is obvious on Grocott and H&E but do not stain on PAS. The micro lab
isolated Absidia spp but the hyphae in the Grocott are septate but I
believe Absidia are aseptate. Any ideas would be most welcome.

Colin Weaver
VLA Thirsk
England



  

Veterinary Laboratories Agency (VLA)

This email and any attachments is intended for the named recipient
only.
If you have received it in error you have no authority to use, disclose,
store or copy any of its contents and you should destroy it and inform
the sender.
Whilst this email and associated attachments will have been checked
for known viruses whilst within VLA systems we can accept no
responsibility once it has left our systems.
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RE: [Histonet] What the heck is this doiing on the HistoNet???

[Mickie Johnson]

Ditto!

Best Regards,
 
Mickie
 
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
  & Mohs Suite Mohs Reporting Software
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX   509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com &
www.mohssuite.com
Email: [EMAIL PROTECTED]
 
DISCLAIMER:
This message is intended for the sole use of the addressee, and may contain
information that is privileged, confidential and exempt from disclosure
under applicable law. If you are not the addressee you are hereby notified
that you may not use, copy, disclose, or distribute to anyone the message or
any information contained in the message. If you have received this message
in error, please immediately advise the sender by reply email and delete
this message. Thank You.
 


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of
[EMAIL PROTECTED]
Sent: Wednesday, November 05, 2008 7:52 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] What the heck is this doiing on the HistoNet???

This kind of material really should not be posted on the HistoNet! Why
don't 
you find some other place to vent!
 
Elfi Hacker
Hacker Industries Inc
 
http://www.youtube.com/watch?v=wFYk7a3vEyo

I don't know how many  times I've heard the words "Obama" and "Messiah" used
in the same sentence.  To liberals, Barack Obama is a spotless god-like
figure who will save America  from the scourge of neo-conservatism. I'm
totally sick of hearing and reading  your hero-worshiping crap, folks. Let's
face reality: *like so many other  powerful politicians in this country,
Barack Obama sucks*:


1. *The  Race Card*: Whether it be in suggesting that anyone who doesn't
vote
for him  because he is black is probably  a
republican,
or  in blaming Bush  administration
racismon  a
slow response to Hurricane Katrina, Obama is quite comfortable  playing
the race card.

2. *Anti-Indian*: After the Obama campaign  released a paper disparaging
other candidates for their ties to the  Indian-American community, the
chairman of the bipartisan US India Political  Action Committee, Sanjay
Puri,
stated that the Obama Campaign was "engaging  in the worst kind of
anti-Indian  American
stereotyping."
Of  course, Obama denied any hand in the racist document put out by  his
campaign.

3. *Corrupt Buddies*: Tony Rezko, a long time friend and  fund-raiser for
Obama, was indicted last fall on federal charges that accuse  him of
demanding kickbacks from companies seeking state business. When asked  about
his friend, Obama said, "I've never done any favors for him." This  turned
out to be a lie, as evidence  turned
upprov
in
g
that  Obama had written letters to city and state officials praising
Rezko's  business practices.

4. *Wal-Mart Ties*: While bashing of Wal-Mart's labor  practices in public,
Obama has been profiting from  their
businessthrough
the  money his wife made as a member of the board of directors for a
company that  produces food for the mega-corporation.

5. *Religious Ties*: Is Obama a  Muslim? Is he a Christian? Nobody is 100%
sure, but it is true that Obama was  raised in a Muslim family and at one
time attended an Islamic school. He  currently claims to be a convert to
Christianity, but some are concerned  about his  Muslim
upbringing
.

6.  *Anti-Second Amendment*: Obama is one of the most anti-Second  Amendment
legislators   in the
country.  He supports a ban the sale or transfer of *all* forms of
semi-automatic  weapons.

7. *Gas-guzzler*: Obama might attack American automakers for not  making
enough environmental friendly automobiles, but when he goes home he  drives
a
gas-guzzling V-8 hemi-powered  Chrysler
300
.

8.  *Obama Ringtones*: The most annoying campaign  tool
ever
.

9.  *Obama Girl*: I take back what I said about the ringtones.  This
girlis far more  annoying.

10. *His Unelectable Name*: Barack Hussein Obama, 'nuff  said.



On Tue, Nov 4, 2008 at 6:47 PM, Linda Jones  
<[EMAIL PROTECTED]>wrote:

>
>  
stupid!!
!!
>
>  Linda Harper-Jones BS.,HT/ HTL(ASCP)
> University of Mississippi Medical  Center
> Department of Pathology

[Histonet] What the heck is this doiing on the HistoNet???

[HACKERLAB]

This kind of material really should not be posted on the HistoNet! Why  don't 
you find some other place to vent!
 
Elfi Hacker
Hacker Industries Inc
 
http://www.youtube.com/watch?v=wFYk7a3vEyo

I don't know how many  times I've heard the words "Obama" and "Messiah" used
in the same sentence.  To liberals, Barack Obama is a spotless god-like
figure who will save America  from the scourge of neo-conservatism. I'm
totally sick of hearing and reading  your hero-worshiping crap, folks. Let's
face reality: *like so many other  powerful politicians in this country,
Barack Obama sucks*:


1. *The  Race Card*: Whether it be in suggesting that anyone who doesn't vote
for him  because he is black is probably  a
republican,
or  in blaming Bush  administration
racismon  a
slow response to Hurricane Katrina, Obama is quite comfortable  playing
the race card.

2. *Anti-Indian*: After the Obama campaign  released a paper disparaging
other candidates for their ties to the  Indian-American community, the
chairman of the bipartisan US India Political  Action Committee, Sanjay Puri,
stated that the Obama Campaign was "engaging  in the worst kind of
anti-Indian  American
stereotyping."
Of  course, Obama denied any hand in the racist document put out by  his
campaign.

3. *Corrupt Buddies*: Tony Rezko, a long time friend and  fund-raiser for
Obama, was indicted last fall on federal charges that accuse  him of
demanding kickbacks from companies seeking state business. When asked  about
his friend, Obama said, "I've never done any favors for him." This  turned
out to be a lie, as evidence  turned
upprovin
g
that  Obama had written letters to city and state officials praising
Rezko's  business practices.

4. *Wal-Mart Ties*: While bashing of Wal-Mart's labor  practices in public,
Obama has been profiting from  their
businessthrough
the  money his wife made as a member of the board of directors for a
company that  produces food for the mega-corporation.

5. *Religious Ties*: Is Obama a  Muslim? Is he a Christian? Nobody is 100%
sure, but it is true that Obama was  raised in a Muslim family and at one
time attended an Islamic school. He  currently claims to be a convert to
Christianity, but some are concerned  about his  Muslim
upbringing
.

6.  *Anti-Second Amendment*: Obama is one of the most anti-Second  Amendment
legislators   in the
country.  He supports a ban the sale or transfer of *all* forms of
semi-automatic  weapons.

7. *Gas-guzzler*: Obama might attack American automakers for not  making
enough environmental friendly automobiles, but when he goes home he  drives a
gas-guzzling V-8 hemi-powered  Chrysler
300
.

8.  *Obama Ringtones*: The most annoying campaign  tool
ever
.

9.  *Obama Girl*: I take back what I said about the ringtones.  This
girlis far more  annoying.

10. *His Unelectable Name*: Barack Hussein Obama, 'nuff  said.



On Tue, Nov 4, 2008 at 6:47 PM, Linda Jones  
<[EMAIL PROTECTED]>wrote:

>
>  
stupid
>
>  Linda Harper-Jones BS.,HT/ HTL(ASCP)
> University of Mississippi Medical  Center
> Department of Pathology
> Chief Histotechnologist  Supervisor
> Telephone (601) 984-1576
> Fax (601) 984-4968
>  [EMAIL PROTECTED]
>
>
> The information contained in  this email is confidential.  Individuals who
> have received this  information in error or are not authorized to receive it
> must promptly  return or dispose of the information and notify the sender.
>  Those  individuals are hereby notified that they are strictly prohibited
> from  reviewing, forwarding, printing, copying, distributing or using this
>  information in any way.
>
>
> >>> "Sam Histology"  <[EMAIL PROTECTED]> 11/04/08 8:48 AM >>>
> Barack  Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama
> Sr.  (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann
>  Dunham
> of Wichita, Kansas. (white atheist ).
>
> When Obama  was two years old, his parents divorced and his father returned
> to  Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta
>  with Obama when he was six years old. Within six months he had learned  to
> speak the Indonesian language. Obama spent "two years in a Muslim  s

[Histonet] Gina Rodriguez is out of the office.

[Gina . Rodriguez]

I will be out of the office starting  11/05/2008 and will not return until
11/10/2008.

I will respond to your message when I return.


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[Histonet] President

[Joachim Hehl]

Interesting, the histonet list seems to be occupied by ultraconservatives.


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[Histonet] Thank you!

[HACKERLAB]

Many thanks for intervening.
 
Elfi Hacker
Hacker Industries
Winnsboro, SC
 

Dear Histonetters:
I am the list administrator and I would like to  ask that people please 
refrain from further political comments on the list.  I know this is a touchy 
topic but this is not the forum for these  discussions.  Please remember, my 
colleagues and I at the University of  Texas Southwestern Medical Center run 
this list using University resources  and it would be a real shame if they 
were to decide this was not a good use  of their funds due to the off-topic 
comments etc. The Histonet list is an  amazing collection of >3300 people 
from around the world helping each  other and we really need to stick to 
topics pertinent to Histology.  Thanks.
Linda M
Histonet administrator

Linda Margraf,  MD
Professor of Pathology
University of Texas Southwestern Medical  School
Dallas TX USA
**AOL Search: Your one stop for directions, recipes and all other 
Holiday needs. Search Now. 
(http://pr.atwola.com/promoclk/10075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from
-aol-search/?ncid=emlcntussear0001)
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[Histonet] prostate core processing

[karen adams]

Greetings,

 Can anyone offer a processing schedual for prostate bx cores for the
Leica processor??
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RE: [Histonet] ASCP HT maintenance fees

[Burnette, Phyllis]

Sorry RC, Now who's being biased and where is the fairness?  You imply
older histotechs do not study hard and receive a salary that they may or
may not be entitled to??  I go to college and take night classes to keep
from being sterotyped this way. I also have staff that I would stand up
for hands down (certified or non-certified) vs someone that just got out
of school, has a lot of textbook knowledge, and is certified. The
heathcare industry regarding Histology is just now becoming acutely
aware of the impact the new changes have made.  We as Histotechs need to
stand together and support one another (certified or
non-certified)through this difficult and stressful time in the
workplace.  Thanks Phyllis  

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of R C
Sent: Monday, November 03, 2008 6:29 PM
To: Podawiltz, Thomas
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] ASCP HT maintenance fees


 Thanks for the many replies however my objective hasn't been addressed.

For the supervisors; My point is not to mislead and claim ASCP
certification without having it. My question is to assist in
rationalizing cost.
Disregarding a credible applicant who doesn't see the financial
investment towards ASCP shouldn't be interpreted as not being serious
about a field.
That ideology promotes "pay to play." It's a direct question towards
ASCP membership feedback and prejudice (pre-2004 certification).
As far as seriousness of the field, revert to my original objective of
understanding what my dues fund, what do I as a tech directly receive in
return, and why are older tech's exempt?

The truth is many techs employed in clinical labs who do not have
certification receive comparable salaries to those histotechs who study
hard, pass the HT exam, and pay ASCP dues. Where is the justification in
that? How does JACHO feel about that?

I pose a credible question of fairness and return investment. Cash is
king now and where I spend it is ever important.




On Mon, Nov 3, 2008 at 12:06 PM, Podawiltz, Thomas
<[EMAIL PROTECTED]>wrote:

> If you were certified in 2004 or after you need to turn in 36 credit 
> hours of continuing education in order to maintain your certification.

> You do not need to be a member of ASCP to be certified, however you do

> get some free CE hours with your membership. My certification was in 
> 85, so yes, I am one of the old farts that is exempt. However, I have 
> stayed current with my education. even in the years that I did not
practice Histology.
>
> As a supervisor, I would not look at a resume that had an expired 
> certification. Right or wrong I would assume that, the applicant did 
> not take this field seriously enough by letting their certification
lapse.
>
> Tom Podawiltz, HT (ASCP)
> Histology Section Head/Laboratory Safety Officer LRGHealthcare
> 603-524-3211 ext: 3220
> 
> From: [EMAIL PROTECTED] [ 
> [EMAIL PROTECTED] On Behalf Of Kimberly 
> Tuttle [ [EMAIL PROTECTED]
> Sent: Monday, November 03, 2008 2:08 PM
> To: R C; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] ASCP HT maintenance fees
>
> Really? I never pay to maintain HT certification. As far as I know 
> theres a ASCP membership fee, but you dont have to be a member to be 
> certified. Am I wrong here?
>
> Kimberly C. Tuttle  HT (ASCP)
> Pathology Biorepository and Research Core University of Maryland Room 
> NBW58, UMMC
> 22 S. Greene St
> Baltimore, MD 21201
> (410) 328-5524
> (410) 328-5508 fax
>
>
> >>> "R C" <[EMAIL PROTECTED]> 11/3/2008 12:49 pm >>>
> Can someone assist me in rationalize the annual cost of maintining HT 
> certification (roughly $100 annually) and its benefit? Point 
> accumulation is generally low for classes you must pay for, and those 
> who obtained certification prior to 2004 are exempt. Should one not 
> pay the annual fee, certification is dropped Is this correct?). In 
> that case, can one advertise "HT" certification for future employment 
> opportunities then, offer full explanation (and expired certification)

> during interview and that be sufficient?
>
> What I generally receive from ASCP is an annual bill and a random 
> newsletter from time to time. Furthermore, when a bill isn't paid on 
> time, the termingology in the subsequent bills become similar to that 
> of a collection agency. Frankly, I find this mailing submission as 
> well as state and national meetings more informative.
>
> Someone please clarify something I might be missing and any benefits 
> of the "pay out."
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> This e-mail and any accompanying attachments may be privileged, 
> confidential, contain protected health information about an identified

> patient or be otherwise protected from disclosure. State and federal 
> law protect the confidenti

Re: [Histonet] funding for NIH

[Geoff McAuliffe]

Hi Emily et al.

The President, any President, can put $ for NIH is the budget request 
he/she submits to Congress but ONLY Congress can appropriate money. That 
is in the Constitution.
Several of my colleagues are out of work due to loss of grant support 
'cause NIH, while not broke, has many worthy projects to fund and cannot 
fund them all.


Geoff

Emily Sours wrote:

Isn't the election over?
Griping will do you no good, spambot!

So, being in grant funded research, I hope our new president will put some
money back into NIH.
(I was hoping that no matter who won, so my political views will not spam
you)
Anyone else feel that way?

Emily
  



--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
[EMAIL PROTECTED]

**



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Re: [Histonet] WOE TO AMERICA!

[Emily Sours]

Isn't the election over?
Griping will do you no good, spambot!

So, being in grant funded research, I hope our new president will put some
money back into NIH.
(I was hoping that no matter who won, so my political views will not spam
you)
Anyone else feel that way?

Emily
-- 
"You would know her for all the things she was...a woman who knew her way in
and out of every new book without being singed, pinched, bumped or tickled
by any line or chapter."
John O'Hara, Appointment in Samarra
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Re: [Histonet] re: Biocare Decloaker HIER

[Linda M Watson]

Is the 118 psi a typo? I don't believe the instrument can go that 
high-max psi is 30.


Piero Nelva wrote:

I used it for 4 minutes at about 118 psi and most antibodies worked 
really well.  I was having huge problems with the Er and Her2 and this 
solved them (almost) overnight.



- Original Message - From: "Jan Shivers" <[EMAIL PROTECTED]>
To: "histonet" 
Sent: Wednesday, November 05, 2008 3:12 AM
Subject: [Histonet] re: Biocare Decloaker HIER


For those of you who use Biocare's Decloaker for HIER on regular cases 
(not prion protein retrieval), could you tell me how long you do HIER 
with these machines?  I normally do my prion cases in a Decloaker for 
20', with a 25' cooldown, but I suspect that regular tissues don't 
need that length of time at high pressure/temp.  I have an extra 
Decloaker and am considering switching to this device for my normal 
case work HIER, if it's time efficient.


Please reply privately.   Thanks.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
[EMAIL PROTECTED]
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