Re: [Histonet] Trichrome Question

[John Kiernan]

Which trichrome method? What kind of section (frozen, paraffin, plastic?)
Any post-fixation in something other than plain formalin before staining? 
Provide the technical details, and you will get lots of advice! 
 
Weeks in formaldehyde is not "prolonged" fixation unless the weeks 
are numerous enough to make years. As a generality, trichrome methods 
do not work very well after fixation in liquids that have formaldehyde as the 
only active ingredient. Postfixation of the sections can compensate. Bouin 
is frequently used. Saturated aqueous picric acid us just as good. There are
published reports that iodine and even citrate buffer will improve trichrome
staining of paraffin sections of formaldehyde-fixed tissue. 
 
"Trichrome" has been applied to several staining techniques that use two 
or more dyes. By convention, since about 1920, trichrome methods have
been those using phosphomolybdic or phosphotungstic acid (or both) to 
enable the staining of collagen and cytoplasm by anionic dyes with sharply 
contrasting colours: blue or green for collagen, and red for cytoplasm
(including smooth & striated muscle). A third anionic dye, typically yellow 
or orange, may be added to stain red blood cells. Instructions for 
trichrome methods can be found in all textbooks of  microtechnique and
histotechnology. 
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: [EMAIL PROTECTED]
Date: Friday, November 7, 2008 20:17
Subject: [Histonet] Trichrome Question
To: histonet@lists.utsouthwestern.edu

> 
> Performed a trichrome stain on a piece of colon which had been 
> in formalin for? weeks.? Muscle stained blue instead of 
> red.?What happened?? Does prolonged fixation react this way?
> Hope someone can shed some light on this.
> Regards,
> Angela
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Advice

[Shelly Coker]

If you are looking for the title/heading for the column, I would suggest 
"Tissue Type Submitted".  Hope this helps.
 
Michelle



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] schiff's

[Anne van Binsbergen]

quick question: does the formalin turn deep magenta or purple when you add
the schiffs
i have had a problem with paths saying they dislike the 'blueish' hues
(purple) of the schiffs in the pas done on the Artisan
we now do them all manually with our in-house schiffs (deep pink/magenta)
no HX overstaining
anyone out there having similar problems?
AbuDhabiAnnie

2008/11/7 Emily Sours <[EMAIL PROTECTED]>

> Schiff's reagent is my nemesis.  It's clear until it gets on anything
> outside of the bottle.
> Including the floor, clothing, and our fume hood.
> (Our post-doc had a little accident.  Actually a rather large one,
> involving
> 1 L of Schiff's.)
>
> Emily
>
> On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger <[EMAIL PROTECTED]>
> wrote:
>
> > We are using Shiffs reagent.  When you put a drop of formalin in Shiffs
> > reagent it turns purple.
> >
> > Roger Charles
> > Microbiologist
> > Pennsylvania Veterinary Laboratory
> > 2305 N Cameron St
> > Harrisburg, PA 17110
> > 717-787-8808
> >
> > -Original Message-
> > From: [EMAIL PROTECTED] [mailto:
> > [EMAIL PROTECTED] On Behalf Of Smith Wanda
> > Sent: Friday, November 07, 2008 1:31 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Is it Formalin-Without Smelling It?
> >
> > Happy Friday Everyone!
> > Silly question:  Is there an easy way to tell if a solution in a
> container
> > is/is not formalin without smelling it?
> > Thanks,
> > Wanda
> >
> > WANDA G. SMITH, HTL(ASCP)HT
> > Pathology Supervisor
> > TRIDENT MEDICAL CENTER
> > 9330 Medical Plaza Drive
> > Charleston, SC  29406
> > 843-847-4586
> > 843-847-4296 fax
> >
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
> "You would know her for all the things she was...a woman who knew her way
> in
> and out of every new book without being singed, pinched, bumped or tickled
> by any line or chapter."
> John O'Hara, Appointment in Samarra
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histonet Digest, Vol 60, Issue 15

[drmoses111]

Position open in our histology department. Good skills required, registered 
HT/HTL  preferred.
Send resume [EMAIL PROTECTED]
-- Original message -- 
From: [EMAIL PROTECTED] 

> Send Histonet mailing list submissions to 
> histonet@lists.utsouthwestern.edu 
> 
> To subscribe or unsubscribe via the World Wide Web, visit 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> or, via email, send a message with subject or body 'help' to 
> [EMAIL PROTECTED] 
> 
> You can reach the person managing the list at 
> [EMAIL PROTECTED] 
> 
> When replying, please edit your Subject line so it is more specific 
> than "Re: Contents of Histonet digest..." 
> 
> 
> 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Trichrome Question

[angelafogg]

Performed a trichrome stain on a piece of colon which had been in formalin for? 
weeks.? Muscle stained blue instead of red.?What happened?? Does prolonged 
fixation react this way?
Hope someone can shed some light on this.
Regards,
Angela
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] schiff's

[Joseph Saby]

I had a little problem once with Schiffs.

I was making up a new batch (I believe about 2 liters) and had just finished 
boiling the water.  I was in a hurry (often the culprit in disasterous lab 
events) and forgat that impure solvents have a lower boiuling point.  When I 
added the basic fuchsin, it erupted like a volcano!

Now, to get the full picture, you must envision a newly remodeled lab.  New 
tile floor.  Newly painted cupboards.  Fresh paint on the wall.  And, of 
course, the lab director a planning walk-through show-off tour of the new 
facility.

Bucket and mop to try to remove most of the excess.  Gallons of acid alcohol.  
And, yes, 10% bleach solution for the fresh paint and the cabinets.  It took 
all day, but I got it looking good as new.

The next week, someone broke a container of eosin on the floor.  My boss 
forever blamed me for the orange-pink arising every time they mopped the 
floor...





From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]>
To: "Swain, Frances L" <[EMAIL PROTECTED]>; Emily Sours <[EMAIL PROTECTED]>; 
histonet@lists.utsouthwestern.edu
Sent: Friday, November 7, 2008 6:05:52 PM
Subject: RE: [Histonet] schiff's



A Liter!!! It took most of a day to clean 40 ml dropped in a dishwasher  to 
which the tech added  extra multiterge, which caused the dishwasher to 
overflow. A colorful event. My sympathy
Rena Fail
-- Original message from "Swain, Frances L" <[EMAIL PROTECTED]>: 
--


> If you have a staining problem from the spill a weak solution of bleach
> usually clears up any Schiff's that is dropped on the bench, floor, hands,
> etc.
> 
> Frances L. Swain HT(ASCP) A. A. S.
> Special Procedures Technician
> Department of Orthopaedic Surgery
> Center for Orthopaedic Research
> Barton Research Building 2R28
> 4301 West Markham Street
> Little Rock AR 72205
> (501) 686-8739 PHONE
> (501) 686-8987 FAX
> [EMAIL PROTECTED] email
> -Original Message-
> From: [EMAIL PROTECTED]
> [mailto:[EMAIL PROTECTED] On Behalf Of Emily Sours
> Sent: Friday, November 07, 2008 1:12 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] schiff's
> 
> Schiff's reagent is my nemesis.  It's clear until it gets on anything
> outside of the bottle.
> Including the floor, clothing, and our fume hood.
> (Our post-doc had a little accident.  Actually a rather large one, involving
> 1 L of Schiff's.)
> 
> Emily
> 
> On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger  wrote:
> 
> > We are using Shiffs reagent.  When you put a drop of formalin in Shiffs
> > reagent it turns purple.
> >
> > Roger Charles
> > Microbiologist
> > Pennsylvania Veterinary Laboratory
> > 2305 N Cameron St
> > Harrisburg, PA 17110
> > 717-787-8808
> >
> > -Original Message-
> > From: [EMAIL PROTECTED] [mailto:
> > [EMAIL PROTECTED] On Behalf Of Smith Wanda
> > Sent: Friday, November 07, 2008 1:31 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Is it Formalin-Without Smelling It?
> >
> > Happy Friday Everyone!
> > Silly question:  Is there an easy way to tell if a solution in a container
> > is/is not formalin without smelling it?
> > Thanks,
> > Wanda
> >
> > WANDA G. SMITH, HTL(ASCP)HT
> > Pathology Supervisor
> > TRIDENT MEDICAL CENTER
> > 9330 Medical Plaza Drive
> > Charleston, SC  29406
> > 843-847-4586
> > 843-847-4296 fax
> >
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> 
> 
> 
> -- 
> "You would know her for all the things she was...a woman who knew her way in
> and out of every new book without being singed, pinched, bumped or tickled
> by any line or chapter."
> John O'Hara, Appointment in Samarra
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> Confidentiality Notice: This e-mail message, including any attachments, is 
> for 
> the sole use of the intended recipient(s) and may contain confidential and 
> privileged information.  Any unauthorized review, use, disclosure or 
> distribution is prohibited.  If you are not the intended recipient, please 
> contact the sender by reply e-mail and destroy all copies of the original 
> message.
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu

RE: [Histonet] schiff's

[renafail]

 
 A Liter!!! It took most of a day to clean 40 ml dropped in a dishwasher   to 
which the tech added  extra multiterge, which caused the dishwasher to 
overflow. A colorful event. My sympathy
Rena Fail
 -- Original message from "Swain, Frances L" <[EMAIL PROTECTED]>: 
--


> If you have a staining problem from the spill a weak solution of bleach
> usually clears up any Schiff's that is dropped on the bench, floor, hands,
> etc.
> 
> Frances L. Swain HT(ASCP) A. A. S.
> Special Procedures Technician
> Department of Orthopaedic Surgery
> Center for Orthopaedic Research
> Barton Research Building 2R28
> 4301 West Markham Street
> Little Rock AR 72205
> (501) 686-8739 PHONE
> (501) 686-8987 FAX
> [EMAIL PROTECTED] email
> -Original Message-
> From: [EMAIL PROTECTED]
> [mailto:[EMAIL PROTECTED] On Behalf Of Emily Sours
> Sent: Friday, November 07, 2008 1:12 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] schiff's
> 
> Schiff's reagent is my nemesis.  It's clear until it gets on anything
> outside of the bottle.
> Including the floor, clothing, and our fume hood.
> (Our post-doc had a little accident.  Actually a rather large one, involving
> 1 L of Schiff's.)
> 
> Emily
> 
> On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger  wrote:
> 
> > We are using Shiffs reagent.  When you put a drop of formalin in Shiffs
> > reagent it turns purple.
> >
> > Roger Charles
> > Microbiologist
> > Pennsylvania Veterinary Laboratory
> > 2305 N Cameron St
> > Harrisburg, PA 17110
> > 717-787-8808
> >
> > -Original Message-
> > From: [EMAIL PROTECTED] [mailto:
> > [EMAIL PROTECTED] On Behalf Of Smith Wanda
> > Sent: Friday, November 07, 2008 1:31 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Is it Formalin-Without Smelling It?
> >
> > Happy Friday Everyone!
> > Silly question:  Is there an easy way to tell if a solution in a container
> > is/is not formalin without smelling it?
> > Thanks,
> > Wanda
> >
> > WANDA G. SMITH, HTL(ASCP)HT
> > Pathology Supervisor
> > TRIDENT MEDICAL CENTER
> > 9330 Medical Plaza Drive
> > Charleston, SC  29406
> > 843-847-4586
> > 843-847-4296 fax
> >
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> 
> 
> 
> -- 
> "You would know her for all the things she was...a woman who knew her way in
> and out of every new book without being singed, pinched, bumped or tickled
> by any line or chapter."
> John O'Hara, Appointment in Samarra
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> Confidentiality Notice: This e-mail message, including any attachments, is 
> for 
> the sole use of the intended recipient(s) and may contain confidential and 
> privileged information.  Any unauthorized review, use, disclosure or 
> distribution is prohibited.  If you are not the intended recipient, please 
> contact the sender by reply e-mail and destroy all copies of the original 
> message.
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] All is revealed.

[Ford Royer]

Ian,

I believe that you were thinking along the correct lines... "unscibe" is
actually the root origin of the modern day word "unsubscribe"  I believe it
comes from the early Pictish language spoken in northern & central Scotland
in the middle ages, meaning: "to doodle without a pict (or Pict)". Your
patois just hasn't made it to the 21st centaury as yet.

Ford 
 
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Ian
Montgomery
Sent: Friday, November 07, 2008 5:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] All is revealed.

Ford,

Many thanks and me thinking it was simply, unscibe.

Ian.

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] RE: Is it Formalin-Without Smelling It?

[Jackie M O'Connor]

Years ago, we added methyl violet to our formalin which enabled us to 
quickly identify the solution in or out of a container.  It also serves as 
a pH indicator, turns yellow when acidic.  That came in handy for long 
term fixation of large bloody specimens that might have required a 
formalin change to ensure good fixation.





JR R <[EMAIL PROTECTED]> 
Sent by: [EMAIL PROTECTED]
11/07/2008 02:21 PM

To

cc

Subject
[Histonet] RE: Is it Formalin-Without Smelling It?








My favorite method is to have the container LABELED.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology



> From: [EMAIL PROTECTED]
> To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu
> Date: Fri, 7 Nov 2008 13:34:43 -0600
> CC: 
> Subject: [Histonet] RE: Is it Formalin-Without Smelling It?
> 
> I knew there was a trick, I just couldn't remember what it was.
> Thanks everyone!
> Wanda 
> 
> 
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
> 
> -Original Message-
> From: [EMAIL PROTECTED] 
[mailto:[EMAIL PROTECTED] On Behalf Of Charles, 
Roger
> Sent: Friday, November 07, 2008 1:43 PM
> To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] RE: Is it Formalin-Without Smelling It?
> 
> We are using Shiffs reagent.  When you put a drop of formalin in Shiffs 
reagent it turns purple.
> 
> Roger Charles
> Microbiologist
> Pennsylvania Veterinary Laboratory
> 2305 N Cameron St
> Harrisburg, PA 17110
> 717-787-8808
> 
> -Original Message-
> From: [EMAIL PROTECTED] 
[mailto:[EMAIL PROTECTED] On Behalf Of Smith 
Wanda
> Sent: Friday, November 07, 2008 1:31 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Is it Formalin-Without Smelling It?
> 
> Happy Friday Everyone!
> Silly question:  Is there an easy way to tell if a solution in a 
container is/is not formalin without smelling it?
> Thanks,
> Wanda
> 
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_
Windows Live Hotmail now works up to 70% faster.
http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008___

Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] RE: Is it Formalin-Without Smelling It?

[AGrobe2555]

In our lab, if it isn't labeled, it gets thrown out.  But we work in  
research and not in a clinical setting
Albert   

Albert C.  Grobe, PhD
Tissue Engineering Lab
International Heart Institute of Montana  Foundation


**AOL Search: Your one stop for directions, recipes and all other 
Holiday needs. Search Now. 
(http://pr.atwola.com/promoclk/10075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from
-aol-search/?ncid=emlcntussear0001)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] RE: Is it Formalin-Without Smelling It?

[Smith Wanda]

What a concept?!?!? 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of JR R
Sent: Friday, November 07, 2008 3:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Is it Formalin-Without Smelling It?



My favorite method is to have the container LABELED.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology



> From: [EMAIL PROTECTED]
> To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu
> Date: Fri, 7 Nov 2008 13:34:43 -0600
> CC: 
> Subject: [Histonet] RE: Is it Formalin-Without Smelling It?
> 
> I knew there was a trick, I just couldn't remember what it was.
> Thanks everyone!
> Wanda
> 
> 
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
> 
> -Original Message-
> From: [EMAIL PROTECTED] 
> [mailto:[EMAIL PROTECTED] On Behalf Of 
> Charles, Roger
> Sent: Friday, November 07, 2008 1:43 PM
> To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] RE: Is it Formalin-Without Smelling It?
> 
> We are using Shiffs reagent.  When you put a drop of formalin in Shiffs 
> reagent it turns purple.
> 
> Roger Charles
> Microbiologist
> Pennsylvania Veterinary Laboratory
> 2305 N Cameron St
> Harrisburg, PA 17110
> 717-787-8808
> 
> -Original Message-
> From: [EMAIL PROTECTED] 
> [mailto:[EMAIL PROTECTED] On Behalf Of Smith 
> Wanda
> Sent: Friday, November 07, 2008 1:31 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Is it Formalin-Without Smelling It?
> 
> Happy Friday Everyone!
> Silly question:  Is there an easy way to tell if a solution in a container 
> is/is not formalin without smelling it?
> Thanks,
> Wanda
> 
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_
Windows Live Hotmail now works up to 70% faster.
http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Job Opening

[JMaslanka]

Good techs are hard to find. St Peters Hospital in Helena Montana has an 
opening for a histotech. Great place to work, even more place to live. If 
interested check out www.stpetes.org



Joe Maslanka
Cyto/Histo Coord.
St Peter's Laboratory

" Not everything that can be counted counts.
  Not everything that counts can be counted."
  Albert Einstein
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] bone histology - preventing artifacts

[Alexandra Meinl]

Hello Histonetters,

When I compare my MMA bone histology with with paraffin bone sections I
always
notice the same artifact in the paraffin sections: The bone lining cells
seem to fall off the surface, I also noticed osteoclasts here and there that
were stripped off.
Bone morphology is good (I used EDTA for decal).
I suspect that maybe the dehydration was too fast for bone (I used the same
protocol as for normal tissue specimens).

What are your experiences with decalcified bone sections?
Do you use a different protocol for your VIP?
Or are these artifacts inevitable?

Thanks,

Alexandra
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Advice

[Kristen Yaros]

Might need to expand that type of system to Ms, Rb, Rt, etc.. Wouldn't want
to look back & wonder if it was a Rabbit or Rat. But yes, it gives you a
starting point!

On 11/7/08, Gudrun Lang <[EMAIL PROTECTED]> wrote:
>
> Our specimen in histo are from regular histo, cyto and morgue. We
> distinguish the incoming number with a letter at the beginning:
> Eg. C 00123/08 or O 000123/08 ... in the IT-System it is found as C0800123
> and so on.
>
> For your specimen it could be M for mouse, R for rabbit, H for human 
> Is that a useful hint?
> Gudrun
>
>
> -Ursprüngliche Nachricht-
> Von: [EMAIL PROTECTED]
> [mailto:[EMAIL PROTECTED] Im Auftrag von Ian
> Montgomery
> Gesendet: Freitag, 07. November 2008 12:45
> An: histonet@lists.utsouthwestern.edu
> Betreff: [Histonet] Advice
>
>Currently, in my tissue log book I have a column headed,
> FIXATION NUMBER. Here I give every new piece of tissue I receive a
> sequential number, year and the next number. Now that my brief includes
> human anatomy I think that this simple term might not be enough. The tissue
> will still have the same number; it's just that I think a new column
> heading
> is needed. What I'm looking for is the term widely used in labs that would
> cover everything from pieces of research mammalian tissue to human
> material.
>
>Isn't it always the simplest things that really tax the brain?
>
>
>
> Dr. Ian Montgomery,
>
> Histotechnology,
>
> I.B.L.S. Support Unit,
>
> Thomson Building,
>
> University of Glasgow,
>
> Glasgow,
>
> G12 8QQ.
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Kristen Yaros, HT (ASCP)CM
Histotechnology Society of Delaware
Correspondence Secretary
[EMAIL PROTECTED]
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Is it Formalin-Without Smelling It?

[JR R]

My favorite method is to have the container LABELED.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology



> From: [EMAIL PROTECTED]
> To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu
> Date: Fri, 7 Nov 2008 13:34:43 -0600
> CC: 
> Subject: [Histonet] RE: Is it Formalin-Without Smelling It?
> 
> I knew there was a trick, I just couldn't remember what it was.
> Thanks everyone!
> Wanda 
> 
> 
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
> 
> -Original Message-
> From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Charles, Roger
> Sent: Friday, November 07, 2008 1:43 PM
> To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] RE: Is it Formalin-Without Smelling It?
> 
> We are using Shiffs reagent.  When you put a drop of formalin in Shiffs 
> reagent it turns purple.
> 
> Roger Charles
> Microbiologist
> Pennsylvania Veterinary Laboratory
> 2305 N Cameron St
> Harrisburg, PA 17110
> 717-787-8808
> 
> -Original Message-
> From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Smith Wanda
> Sent: Friday, November 07, 2008 1:31 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Is it Formalin-Without Smelling It?
> 
> Happy Friday Everyone!
> Silly question:  Is there an easy way to tell if a solution in a container 
> is/is not formalin without smelling it?
> Thanks,
> Wanda
> 
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_
Windows Live Hotmail now works up to 70% faster.
http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] 200 proof ethanol

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

Many, many years ago I worked with a pathologist that told of parties
with the waste (eosin-tinted) alcohols...and he discovered that when
he sweated he sweated pinkish-red! 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
[EMAIL PROTECTED]


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of
powell_sa
Sent: Friday, November 07, 2008 2:54 PM
To: Breeden, Sara; histonet
Subject: RE: [Histonet] 200 proof ethanol

Got you beat by 6 years Sara, and I remember all those fun things too,
except I did not visit the bees. And the reason you colored the alcohol
was to keep the interns and residents from having a party and using it
all up. I also remember making hematoxylin and our ceiling had a huge
purple circle right over the counter where we made it and "blew" it up.
:)  Those were the good ole days, well old anyway.

Shirley
>= Original Message From "Breeden, Sara" <[EMAIL PROTECTED]> 
>= I remember in the Olde Days (1971-76 or so) we got our ethanol in

>50 gallon drums and it was my job to color the alcohol with something 
>pink so it would be "recognized".  Don't remember what the "pink" stuff

>was and I'm sure there was a reason for coloring it but I was young and

>didn't think I needed to know that, apparently.  I did that, as I 
>remember, after gathering eggs from the chicken house for the albumen 
>and fighting the bees off for the wax... nah! - I'm not THAT old!  I do

>remember round snap cassettes and L-shaped molds and sharpening knives 
>on a glass plate.. maybe it's time for me to retire.  Come to think of 
>it - my ASCP certificate tells me that on March 7, 2009, I will have 
>been practicing histology for FORTY years.  Maybe I do remember the wax

>and chickens... And maybe I need to keep practicing!  That's my Friday 
>Fume for this L-O-N-G very interesting week in the world... adios.
>
>
>
>Sally Breeden, HT(ASCP)
>
>NM Dept. of Agriculture
>
>Veterinary Diagnostic Services
>
>PO Box 4700
>
>Albuquerque, NM  87106
>
>505-841-2576
>
>
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: 200Proof

[Hobbs, Carl]

Just to try to help to make clear to those of us who get confused when people 
talk about "proof" and % alcohol, I would recommend reading : 
http://en.wikipedia.org/wiki/Proof_spirit
Proof is all about NOT getting a BANG from gunpowder ;-)

In UK Histopath/Histology labs, we traditionally use 64OP and/or 74OP Spirit, 
to confuse the post a little: this is NOT pure ethanol but Industrial 
Methylated Spirit (IMS).
OP= Over-Proof, an antiquated English Customs and Excise term but, a nice and 
precise term/measure.
 If you want to test it with Gunpowder to make sure.you run the risk of 
being arrested as a terrorist.

IMS does not incur taxation/regulation , in the way that ethanol does, because 
Methanol is added to the Ethanol base. ( under increasing EU regulations I may 
be wrong,now)
Anyone who uses Ethanol for tissue processing/de-rehydrating is incurring, in 
many  Countries, imho,  extra irrelevant costs.
Sure, many Histonetters do not have a choice.
Carl

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] 200 proof ethanol

[Yaskovich, Ruth A (NIH/NIDCR) [E]]

I remember working for the Maryland Department of Agriculture and our
formalin was pink. I think it was actually Embalming Fluid!
Ruth Yaskovich
N.I.H.
National Institute of Dental and Crainiofacial Research
Neurobiology and Pain Branch

-Original Message-
From: powell_sa [mailto:[EMAIL PROTECTED] 
Sent: Friday, November 07, 2008 2:54 PM
To: Breeden, Sara; histonet
Subject: RE: [Histonet] 200 proof ethanol

Got you beat by 6 years Sara, and I remember all those fun things too,
except 
I did not visit the bees. And the reason you colored the alcohol was to
keep 
the interns and residents from having a party and using it all up. I
also 
remember making hematoxylin and our ceiling had a huge purple circle
right 
over the counter where we made it and "blew" it up. :)  Those were the
good 
ole days, well old anyway.

Shirley
>= Original Message From "Breeden, Sara" <[EMAIL PROTECTED]>
=
>I remember in the Olde Days (1971-76 or so) we got our ethanol in 50
>gallon drums and it was my job to color the alcohol with something pink
>so it would be "recognized".  Don't remember what the "pink" stuff was
>and I'm sure there was a reason for coloring it but I was young and
>didn't think I needed to know that, apparently.  I did that, as I
>remember, after gathering eggs from the chicken house for the albumen
>and fighting the bees off for the wax... nah! - I'm not THAT old!  I do
>remember round snap cassettes and L-shaped molds and sharpening knives
>on a glass plate.. maybe it's time for me to retire.  Come to think of
>it - my ASCP certificate tells me that on March 7, 2009, I will have
>been practicing histology for FORTY years.  Maybe I do remember the wax
>and chickens... And maybe I need to keep practicing!  That's my Friday
>Fume for this L-O-N-G very interesting week in the world... adios.
>
>
>
>Sally Breeden, HT(ASCP)
>
>NM Dept. of Agriculture
>
>Veterinary Diagnostic Services
>
>PO Box 4700
>
>Albuquerque, NM  87106
>
>505-841-2576
>
>
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] 200 proof ethanol

[powell_sa]

Got you beat by 6 years Sara, and I remember all those fun things too, except 
I did not visit the bees. And the reason you colored the alcohol was to keep 
the interns and residents from having a party and using it all up. I also 
remember making hematoxylin and our ceiling had a huge purple circle right 
over the counter where we made it and "blew" it up. :)  Those were the good 
ole days, well old anyway.

Shirley
>= Original Message From "Breeden, Sara" <[EMAIL PROTECTED]> =
>I remember in the Olde Days (1971-76 or so) we got our ethanol in 50
>gallon drums and it was my job to color the alcohol with something pink
>so it would be "recognized".  Don't remember what the "pink" stuff was
>and I'm sure there was a reason for coloring it but I was young and
>didn't think I needed to know that, apparently.  I did that, as I
>remember, after gathering eggs from the chicken house for the albumen
>and fighting the bees off for the wax... nah! - I'm not THAT old!  I do
>remember round snap cassettes and L-shaped molds and sharpening knives
>on a glass plate.. maybe it's time for me to retire.  Come to think of
>it - my ASCP certificate tells me that on March 7, 2009, I will have
>been practicing histology for FORTY years.  Maybe I do remember the wax
>and chickens... And maybe I need to keep practicing!  That's my Friday
>Fume for this L-O-N-G very interesting week in the world... adios.
>
>
>
>Sally Breeden, HT(ASCP)
>
>NM Dept. of Agriculture
>
>Veterinary Diagnostic Services
>
>PO Box 4700
>
>Albuquerque, NM  87106
>
>505-841-2576
>
>
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] 200 proof ethanol

[Breeden, Sara]

I remember in the Olde Days (1971-76 or so) we got our ethanol in 50
gallon drums and it was my job to color the alcohol with something pink
so it would be "recognized".  Don't remember what the "pink" stuff was
and I'm sure there was a reason for coloring it but I was young and
didn't think I needed to know that, apparently.  I did that, as I
remember, after gathering eggs from the chicken house for the albumen
and fighting the bees off for the wax... nah! - I'm not THAT old!  I do
remember round snap cassettes and L-shaped molds and sharpening knives
on a glass plate.. maybe it's time for me to retire.  Come to think of
it - my ASCP certificate tells me that on March 7, 2009, I will have
been practicing histology for FORTY years.  Maybe I do remember the wax
and chickens... And maybe I need to keep practicing!  That's my Friday
Fume for this L-O-N-G very interesting week in the world... adios.

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Is it Formalin-Without Smelling It?

[Smith Wanda]

I knew there was a trick, I just couldn't remember what it was.
Thanks everyone!
Wanda 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Charles, Roger
Sent: Friday, November 07, 2008 1:43 PM
To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Is it Formalin-Without Smelling It?

We are using Shiffs reagent.  When you put a drop of formalin in Shiffs reagent 
it turns purple.

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Smith Wanda
Sent: Friday, November 07, 2008 1:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Is it Formalin-Without Smelling It?

Happy Friday Everyone!
Silly question:  Is there an easy way to tell if a solution in a container 
is/is not formalin without smelling it?
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] trichromes on ventana nexeES

[anita dudley]

we are doing trichromes for our microbiology dept on their stool samples.  is 
anyone doing this?  we are having trouble with the controls that they purchase 
staining on the slide.  just wondering if anyone else is doing this and what 
they have done to keep the spec on the slide.  the pt slides seem to be fine.
thanks a lot,   everyone have a good weekend!!!   
 
anita dudley
providence hosp
mobile alabama
_
See how Windows® connects the people, information, and fun that are part of 
your life
http://clk.atdmt.com/MRT/go/119463819/direct/01/___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] schiff's

[Swain, Frances L]

If you have a staining problem from the spill a weak solution of bleach
usually clears up any Schiff's that is dropped on the bench, floor, hands,
etc.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
[EMAIL PROTECTED] email
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Emily Sours
Sent: Friday, November 07, 2008 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] schiff's

Schiff's reagent is my nemesis.  It's clear until it gets on anything
outside of the bottle.
Including the floor, clothing, and our fume hood.
(Our post-doc had a little accident.  Actually a rather large one, involving
1 L of Schiff's.)

Emily

On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger <[EMAIL PROTECTED]> wrote:

> We are using Shiffs reagent.  When you put a drop of formalin in Shiffs
> reagent it turns purple.
>
> Roger Charles
> Microbiologist
> Pennsylvania Veterinary Laboratory
> 2305 N Cameron St
> Harrisburg, PA 17110
> 717-787-8808
>
> -Original Message-
> From: [EMAIL PROTECTED] [mailto:
> [EMAIL PROTECTED] On Behalf Of Smith Wanda
> Sent: Friday, November 07, 2008 1:31 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Is it Formalin-Without Smelling It?
>
> Happy Friday Everyone!
> Silly question:  Is there an easy way to tell if a solution in a container
> is/is not formalin without smelling it?
> Thanks,
> Wanda
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
"You would know her for all the things she was...a woman who knew her way in
and out of every new book without being singed, pinched, bumped or tickled
by any line or chapter."
John O'Hara, Appointment in Samarra
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information.  Any unauthorized review, use, disclosure or 
distribution is prohibited.  If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of the original 
message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] schiff's

[Emily Sours]

Schiff's reagent is my nemesis.  It's clear until it gets on anything
outside of the bottle.
Including the floor, clothing, and our fume hood.
(Our post-doc had a little accident.  Actually a rather large one, involving
1 L of Schiff's.)

Emily

On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger <[EMAIL PROTECTED]> wrote:

> We are using Shiffs reagent.  When you put a drop of formalin in Shiffs
> reagent it turns purple.
>
> Roger Charles
> Microbiologist
> Pennsylvania Veterinary Laboratory
> 2305 N Cameron St
> Harrisburg, PA 17110
> 717-787-8808
>
> -Original Message-
> From: [EMAIL PROTECTED] [mailto:
> [EMAIL PROTECTED] On Behalf Of Smith Wanda
> Sent: Friday, November 07, 2008 1:31 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Is it Formalin-Without Smelling It?
>
> Happy Friday Everyone!
> Silly question:  Is there an easy way to tell if a solution in a container
> is/is not formalin without smelling it?
> Thanks,
> Wanda
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
"You would know her for all the things she was...a woman who knew her way in
and out of every new book without being singed, pinched, bumped or tickled
by any line or chapter."
John O'Hara, Appointment in Samarra
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Mouse antibody to fibroblast without human crossreactivity?

[Patsy Ruegg]

I do not think a marker like that exists, if it does I would like to know
about it too.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
[EMAIL PROTECTED]
www.ihctech.net
www.ihcrg.org
 


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Suhyoung
Jeong
Sent: Thursday, November 06, 2008 1:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mouse antibody to fibroblast without human
crossreactivity?

Hello everyone,

I am looking for a primary antibody that will recognize a protein in mouse
fibroblast without crossreactivity to human. If you have any experience in
this matter, please kindly let me know.

Thank you in advance

Suh
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] RE:CD4 on Ventana

[Patsy Ruegg]

True the 4b12 cd4 clone performs better in paraffin, still as you say, cd4
is just not the most robust ab in my hands either, one thing I have found
(actually I need to credit Bryan Hewlett for this), is that you need to do
endogenous h202 block after hier and you should use h202 in methanol,
perhaps it is something about the methanol fixing after hier for ar that
helps with cd4.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
[EMAIL PROTECTED]
www.ihctech.net
www.ihcrg.org
 


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Milne, Katy
Sent: Friday, November 07, 2008 9:21 AM
To: histonet@lists.utsouthwestern.edu; [EMAIL PROTECTED]
Subject: [Histonet] RE:CD4 on Ventana

Hi Piero, what clone are you using for CD4?

There seem to be 2 main clones, 1F6 or something like that and 4B12.
The epitope recognized by 1F6 doesn't do well in formalin so 4B12 is the
one most used.  Having said that, I've found it's still not wonderful, I
can get staining (I use a Ventana Discovery) but because the Ab is
recommended to be used at such a high concentration, you do have to pick
out the real staining against some background.

I used it in this paper, our general protocol is in there too but I'm
not sure how much the benchmark differs from the discovery
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00034
09

Katy Milne 
Deeley Research Centre

Message: 19
Date: Fri, 7 Nov 2008 20:15:11 +1100
From: "Piero Nelva" <[EMAIL PROTECTED]>
Subject: [Histonet] CD4 on Ventana
To: 
Message-ID: <[EMAIL PROTECTED]>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original

Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Liver

[Patsy Ruegg]

Mike are you using formalin fixed paraffin embedded (ffpe) tissue for the IF
staining?
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
[EMAIL PROTECTED]
www.ihctech.net
www.ihcrg.org
 


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Mike Tighe
Sent: Friday, November 07, 2008 8:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Liver

I am am having a difficult time getting immuno fluorescent staining in mouse
liver. I am using markers that work side by side in lung tissue (F4/80,
GR1). Does anyone have any suggestions for Immuno Fluorescent staining in
mouse tissue or similar problems?

Thanks!!


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Is it Formalin-Without Smelling It?

[Charles, Roger]

We are using Shiffs reagent.  When you put a drop of formalin in Shiffs reagent 
it turns purple.

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Smith Wanda
Sent: Friday, November 07, 2008 1:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Is it Formalin-Without Smelling It?

Happy Friday Everyone!
Silly question:  Is there an easy way to tell if a solution in a container 
is/is not formalin without smelling it?
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Is it Formalin-Without Smelling It?

[Smith Wanda]

Happy Friday Everyone!
Silly question:  Is there an easy way to tell if a solution in a container 
is/is not formalin without smelling it?
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE:CD4 on Ventana

[Milne, Katy]

Hi Piero, what clone are you using for CD4?

There seem to be 2 main clones, 1F6 or something like that and 4B12.
The epitope recognized by 1F6 doesn't do well in formalin so 4B12 is the
one most used.  Having said that, I've found it's still not wonderful, I
can get staining (I use a Ventana Discovery) but because the Ab is
recommended to be used at such a high concentration, you do have to pick
out the real staining against some background.

I used it in this paper, our general protocol is in there too but I'm
not sure how much the benchmark differs from the discovery
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00034
09

Katy Milne 
Deeley Research Centre

Message: 19
Date: Fri, 7 Nov 2008 20:15:11 +1100
From: "Piero Nelva" <[EMAIL PROTECTED]>
Subject: [Histonet] CD4 on Ventana
To: 
Message-ID: <[EMAIL PROTECTED]>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original

Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

AW: [Histonet] CD4 on Ventana

[Gudrun Lang]

CD4
4B12Novocastra  NCL-L-CD4-368
40 min, 37°C, Amplifier
CC1 mild
1:20 (I not absolutly sure if I remember the titer right)

Gudrun 

-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Piero
Nelva
Gesendet: Freitag, 07. November 2008 10:15
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] CD4 on Ventana

Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

AW: [Histonet] Advice

[Gudrun Lang]

Our specimen in histo are from regular histo, cyto and morgue. We
distinguish the incoming number with a letter at the beginning:
Eg. C 00123/08 or O 000123/08 ... in the IT-System it is found as C0800123
and so on.

For your specimen it could be M for mouse, R for rabbit, H for human 
Is that a useful hint?
Gudrun


-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Ian
Montgomery
Gesendet: Freitag, 07. November 2008 12:45
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Advice

Currently, in my tissue log book I have a column headed,
FIXATION NUMBER. Here I give every new piece of tissue I receive a
sequential number, year and the next number. Now that my brief includes
human anatomy I think that this simple term might not be enough. The tissue
will still have the same number; it's just that I think a new column heading
is needed. What I'm looking for is the term widely used in labs that would
cover everything from pieces of research mammalian tissue to human material.

Isn't it always the simplest things that really tax the brain? 

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Liver

[Mike Tighe]

I am am having a difficult time getting immuno fluorescent staining in mouse 
liver. I am using markers that work side by side in lung tissue (F4/80, GR1). 
Does anyone have any suggestions for Immuno Fluorescent staining in mouse 
tissue or similar problems?

Thanks!!


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Gram stain on tissue

[Valentin Neubert]

Thank you for all replies.

Just a quick update: I have carbol-gentiana-violet, of course, I just forgot to 
mention it.

Please tell me how to decolorize the fuchsin without affecting the violet, that 
would help me very much in this moment. Thank you!

I'll post my results and my method of course as soon as I get good results.
___
Jetzt neu! Schützen Sie Ihren PC mit McAfee und WEB.DE. 30 Tage
kostenlos testen. http://www.pc-sicherheit.web.de/startseite/?mc=00


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] 200 proof alcohol?

[Merced Leiker]

Ahhh...they are federally regulated...



--On Friday, November 07, 2008 9:29 AM -0500 Merced Leiker 
<[EMAIL PROTECTED]> wrote:



At our university in Buffalo, NY, we have to sign out the alcohol we
purchase from the university (we could buy it from Sigma or other places,
I suppose, but generally want to avoid the SH fee). I was told the State
of NY required the signature...why? So if we're caught driving drunk they
can link it to the alcohol we purchased through the university and
diluted to have a party?? And the university would be held liable??  ;-)

Merced

--On Friday, November 07, 2008 8:56 AM -0500 Emily Sours
<[EMAIL PROTECTED]> wrote:


Our alcohol (95 to 100%) is sold by the university only, we don't get a
choice.  Hopefully if I ever need to use molecular biology grade, the
stuff they sell will be good enough.  Is it regulated in other
universities? I have no idea why they do this, unless they think we're
going to have parties.
Actually, in PA you can't buy grain liquor, so maybe that's why.

Emily
--
"You would know her for all the things she was...a woman who knew her way
in and out of every new book without being singed, pinched, bumped or
tickled by any line or chapter."
John O'Hara, Appointment in Samarra
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





Merced M Leiker
Research Technician II
354 BRB (pkgs) / 140 Farber Hall (letters)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

"Without my flaws I'm really very boring."
- random internet blog commentator


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





Merced M Leiker
Research Technician II
354 BRB (pkgs) / 140 Farber Hall (letters)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

"Without my flaws I'm really very boring."
- random internet blog commentator 
___

Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] CD4 on Ventana

[Sebree Linda A.]

Piero,

We use C4d from American Research Products, Inc., cat. #: 12-5 at
1:50.  On the BenchMark XT we use mild CC1 for 32" at 42 degrees C with
AB block.

Hope this helps.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Piero
Nelva
Sent: Friday, November 07, 2008 3:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD4 on Ventana

Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] 200 proof alcohol?

[Merced Leiker]

At our university in Buffalo, NY, we have to sign out the alcohol we 
purchase from the university (we could buy it from Sigma or other places, I 
suppose, but generally want to avoid the SH fee). I was told the State of 
NY required the signature...why? So if we're caught driving drunk they can 
link it to the alcohol we purchased through the university and diluted to 
have a party?? And the university would be held liable??  ;-)


Merced

--On Friday, November 07, 2008 8:56 AM -0500 Emily Sours 
<[EMAIL PROTECTED]> wrote:



Our alcohol (95 to 100%) is sold by the university only, we don't get a
choice.  Hopefully if I ever need to use molecular biology grade, the
stuff they sell will be good enough.  Is it regulated in other
universities? I have no idea why they do this, unless they think we're
going to have parties.
Actually, in PA you can't buy grain liquor, so maybe that's why.

Emily
--
"You would know her for all the things she was...a woman who knew her way
in and out of every new book without being singed, pinched, bumped or
tickled by any line or chapter."
John O'Hara, Appointment in Samarra
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





Merced M Leiker
Research Technician II
354 BRB (pkgs) / 140 Farber Hall (letters)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

"Without my flaws I'm really very boring."
- random internet blog commentator


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Gram stain on tissue

[Rene J Buesa]

Valentin:
Later in the day I will send you my procedure. It always worked very well for 
me.
René J.

--- On Fri, 11/7/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:

From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
Subject: [Histonet] Gram stain on tissue
To: histonet@lists.utsouthwestern.edu
Date: Friday, November 7, 2008, 8:41 AM

Hello!

To introduce myself: I've been reading this list for the last ~350 mails. I
am a technical assistent, working in vet histo.

I am quite inexperienced with staining, and one task is to get Gram staining to
work. Right now, I've been trying to get results for 2! days, I tried about
10 different ways.

If someone could share a method which has proven to be working, I'd be very
happy.

Reagents available to work with right now: xylene, acetone, picric acid, carbol
fuchsin, basic fuchsin, safranine, fuchsin 1:10.

I'd be glad if I had not to order any more extra reagents.

Thank you for replying.

Greets from Germany,

Valentin
__
"Run, Fatboy, Run" sowie "Rails & Ties" kostenlos
anschauen!
Blockbuster-Gutscheine sichern unter http://www.blockbuster.web.de


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] 200 proof alcohol?

[Emily Sours]

Our alcohol (95 to 100%) is sold by the university only, we don't get a
choice.  Hopefully if I ever need to use molecular biology grade, the stuff
they sell will be good enough.  Is it regulated in other universities?
I have no idea why they do this, unless they think we're going to have
parties.
Actually, in PA you can't buy grain liquor, so maybe that's why.

Emily
-- 
"You would know her for all the things she was...a woman who knew her way in
and out of every new book without being singed, pinched, bumped or tickled
by any line or chapter."
John O'Hara, Appointment in Samarra
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Fatty tissue

[Rene J Buesa]

The MW irradiation will NOT accelerate formalin cross linking; essentially MW 
irradiation will have a "cooking" effect on tissue, that is why the "6-48" rule 
was issued by ASCO-CAP
After MW irradiation the tissues that go into dehydration will be only 
partially fixed with formalin and will end being fixed by the alcohols in a "2 
fixation" process with unknown consequences for the procedures that follow.The 
anecdotal "successful" use of MW for tissue fixation are just that, anecdotal,
René J.

--- On Thu, 11/6/08, Luck, Greg D. <[EMAIL PROTECTED]> wrote:

From: Luck, Greg D. <[EMAIL PROTECTED]>
Subject: RE: [Histonet] Fatty tissue
To: "Tony Henwood" <[EMAIL PROTECTED]>, "Webb, Dorothy L" <[EMAIL PROTECTED]>, 
histonet@lists.utsouthwestern.edu
Date: Thursday, November 6, 2008, 7:14 PM

Hello,
Are people microwave fixing breast tissues?  Just wondering as/does this
fly in the face of the "6-48" hour fixation requirement for any
breast
tissue that is to have Her2-neu testing?
Thanks, Greg





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Gram stain on tissue

[v . neubert]

Hello!

To introduce myself: I've been reading this list for the last ~350 mails. I am 
a technical assistent, working in vet histo.

I am quite inexperienced with staining, and one task is to get Gram staining to 
work. Right now, I've been trying to get results for 2! days, I tried about 10 
different ways.

If someone could share a method which has proven to be working, I'd be very 
happy.

Reagents available to work with right now: xylene, acetone, picric acid, carbol 
fuchsin, basic fuchsin, safranine, fuchsin 1:10.

I'd be glad if I had not to order any more extra reagents.

Thank you for replying.

Greets from Germany,

Valentin
__
"Run, Fatboy, Run" sowie "Rails & Ties" kostenlos anschauen!
Blockbuster-Gutscheine sichern unter http://www.blockbuster.web.de


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] All is revealed.

[Ian Montgomery]

Ford,

Many thanks and me thinking it was simply, unscibe.

Ian.

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Advice

[Ian Montgomery]

Currently, in my tissue log book I have a column headed,
FIXATION NUMBER. Here I give every new piece of tissue I receive a
sequential number, year and the next number. Now that my brief includes
human anatomy I think that this simple term might not be enough. The tissue
will still have the same number; it's just that I think a new column heading
is needed. What I'm looking for is the term widely used in labs that would
cover everything from pieces of research mammalian tissue to human material.

Isn't it always the simplest things that really tax the brain? 

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD4 on Ventana

[Piero Nelva]

Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.


Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet