[Histonet] Staining fungi with Warthin-Starry

[V . Neubert]

Just a quick question:

Did anyone successfully try to stain fungi with Warthin-Starry silver technique?

Thank you for our answers,

V. Neubert


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Fontana Masson

[John Kiernan]

   Probably the best place to start is in Pearse's "Histochemistry"
   4=  th ed Vol 2 p.881 (1985). There is plenty of other interesting
   stuff=  about  melanin  in  the  same chapter. There's also Lillie
   & Fu= llmer's "Histopathologic Technic ..." 4th ed
   p.522-527  (19=  76).  Unfortunately  neither  book  explains  the
   chemistry  of  the  argentaf=  fin  reaction  of melanin. Pearse and
   Lillie  assumed  that  their  readers = knew about tests and reactions
   that  had  been  in  use  for  100+ years! Unfo= rtunately this simple
   chemical  information  is  not in many modern chemist= ry textbooks.
   older  ones  are  more helpful.  = You will
   need  to  follow  up  the  references  cited by Pearse and Lillie.
If   Unilever's library does not have these books, it's
   a =tinyinvestment(perhaps$200)for a
   huge company.=    John
   KiernanAnatomy,  UWOLond= on, Canada.=
   =   =-   Original   Message   -<=   BR>From:
   "Kalleberg,  Kristopher"  <[EMAIL PROTECTED]   NILEVER.COM>Date: 
   Tuesday,   December   2,   2008
   15:04<= BR>Subject: [Histonet] Fontana MassonTo:
   [EMAIL PROTECTED]  sts.utsouthwestern.edu>
   All,>=  > Does anyone have a reference
   for  me  to  read  up  on  the = chemistry > involvedin
   Fontana Masson staining of melanin= ? I am having > a
   hard  time  finding>  in = depth information on exactly
   howFMstainstains   the   >   me=   lanosmes
   and>  the  chemistry  and  reactions  involved.  Any   information,   
  >papers,or>
   references=   would   be   greatly   appreciated.   Thank   you   in
   advance.>=>   Kris
   Kalleberg>ResearchScientist>
   UnileverR&D>   40   Merritt   Blvd.> Trumbull,  
   CT 06611> (203)
   381-5765&= gt;
   __   ___   5F=_>   
Histonet   mailing   list>
   [EMAIL PROTECTED]  ts.utsouthwestern.edu>
   http://lists.utsouthwester   n.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Casual Histotech

[sheila adey]

I can't believe so many people are not familiar with the term "Casual" it could 
also be called contingent. Sheila Adey HT MLT Port Huron Hospital Michigan> 
Date: Tue, 2 Dec 2008 10:43:35 -0500> From: [EMAIL PROTECTED]> To: [EMAIL 
PROTECTED]; Histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Casual 
Histotech> CC: > > As opposed to the rest of us...you know, the formal ones. > 
> > Jeanine Bartlett> Infectious Diseases Pathology Branch> (404) 639-3590 > 
[EMAIL PROTECTED]> > > -Original Message-> From: [EMAIL PROTECTED]> 
[mailto:[EMAIL PROTECTED] On Behalf Of JD> Sent: Tuesday, December 02, 2008 
10:42 AM> To: Histonet@lists.utsouthwestern.edu> Subject: [Histonet] Casual 
Histotech> > My thoughts exactly. Casual> 
histotech?___> Histonet mailing 
list> Histonet@lists.utsouthwestern.edu> 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > 
___> Histonet mailing list> 
Histonet@lists.utsouthwestern.edu> 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Large Slide Boxes

[Joe Nocito]

have you tried Lab Storages Systems, New Comer's Supply or Surgipath?

JTT
- Original Message - 
From: "Pamela Marcum" <[EMAIL PROTECTED]>

To: 
Sent: Tuesday, December 02, 2008 3:40 PM
Subject: [Histonet] Large Slide Boxes




Good Afternoon All,



I am looking to purchase some 3 inch by 2 inch (3X2) slide boxes for large 
slides. We do some larger specimens and it seems these have become almost 
impossible to find. I have tried several sources and no one had them or knew 
where I could get them at this time. I have tried myneurolab and several 
other speciality vendors also to no avail. HELP




Pam Marcum
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ventana Benchmark XTs

[Joe Nocito]

I have to agree with Al ( can I call you Al, Alias?) on this one. Remember 
when I had to sign off of the Histonet a couple of years ago because a 
certain company contacted my CEO and filed a complaint. I almost lost my job 
because I posted an honest opinion. An opinion that was greatly shared if I 
remember correctly. In this era of financial uncertainties, foreclosures and 
all, I don't blame Al. I'm sure he/she has bills just the rest of us do. 
Bills that won't go away if he/she loses the job.

That's my story and I'm sticking to it.

Joe Nocito BS, PA, HT(ASCP)QIHC
San Antonio, TX

- Original Message - 
From: "Histonet Alias" <[EMAIL PROTECTED]>

To: "Della Speranza, Vinnie" <[EMAIL PROTECTED]>
Cc: ; "Tracey Lenek" 
<[EMAIL PROTECTED]>; "Burton,Lynn" <[EMAIL PROTECTED]>; "Joanna 
Bartczak-McKay" <[EMAIL PROTECTED]>; "Martin Trotter" 
<[EMAIL PROTECTED]>

Sent: Tuesday, December 02, 2008 1:19 PM
Subject: Re: [Histonet] Ventana Benchmark XTs


Mr. Della Speranza, I respect what you have done for the histology 
community
and we have had pleasant conversations in the past but you can not speak 
for
the Histonet members. You act as if I am hiding my identity to create 
havoc

and disruption. I have not done this nor will I do this. I have been on
histonet for many years and I will continue to do so. As I explained in my
previous posts my current employer does not want any opinions, help, or
questions associated with the laboratory. That is the only reason that I
have chosen to remain anonymous. Would it make anyone feel better if I 
made

up a fake name and lab as my signature?  I will continue to post and speak
freely without breaking any rules of this listserver. If I respond or ask 
a

question, those who find my years of experience not "credible" then
I apologize.

On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie 
<[EMAIL PROTECTED]>wrote:



I'm afraid your opinion will not be credible as long as you insist on
remaining anonymous. It has nothing to do with whether you like or 
dislike a

particular product or instrument.  Histonet members appreciate the
responsibility that comes with posting one's views or knowledge by
identifying themselves accordingly. In my opinion anyone who insists on
remaining anonymous should not be permitted to participate on the list.


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974


-Original Message-
From: [EMAIL PROTECTED] [mailto:
[EMAIL PROTECTED] On Behalf Of Histonet Alias
Sent: Saturday, November 29, 2008 11:03 AM
To: Mark Tarango
Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
Burton, Lynn; Joanna Bartczak-McKay
Subject: Re: [Histonet] Ventana Benchmark XTs

Sorry I don't work for Ventana and I have no beef with them. It
may disappoint some that I have not had a bad experience. So my opinion
does
not count because I have had a different experience with my XT than you? 
I
use the name of The Unknown HT so I can express my opinions freely 
without

my employer receiving phone calls because certain people don't like my
opinions.

On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango <[EMAIL PROTECTED]>
wrote:

> I don't know how much the opinion of "The Unknown HT(ASCP)" counts. 
> You

> aren't with Ventana I hope.
>
> Mark
>
> On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias <
[EMAIL PROTECTED]>wrote:
>
>> They are switching to a more reliable manufacturer. They are being
>> proactive
>> and switching them before they fail down the road. My XT has been a
>> workhorse but we know certain peoples opinions about the company on
here.
>> I
>> will take it any day over the rest.
>>
>> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn 
>> <[EMAIL PROTECTED]

>> >wrote:
>>
>> > We have had some trouble but they are planning to replace them. We
>> received
>> > a letter that they had changed vendors for parts and found they need
to
>> > switch back. They did replace one section earlier when it stopped
>> working
>> > completely.
>> >
>> > Lynn Burton
>> > Lab Assoc. I
>> > Animal Disease Lab
>> > Galesburg, Il 61401
>> >
>> > 
>> >
>> > From: [EMAIL PROTECTED] on behalf of Tracey
>> Lenek
>> > Sent: Wed 11/26/2008 10:59 AM
>> > To: histonet@lists.utsouthwestern.edu
>> > Cc: Martin Trotter; Joanna Bartczak-McKay
>> > Subject: [Histonet] Ventana Benchmark XTs
>> >
>> >
>> >
>> > Hi,
>> >
>> > We have had on-going thermal slide pad issues with our XTs since 
>> > they

>> were
>> > installed
>> > in February. The slide tray assemblies have been replaced not once 
>> > but

>> > twice and we are still having inconsistent
>> > results with the temp verifier slides.  Has anyone experienced the
same
>> > issues with this
>> > instrumentation?
>> >
>> > Thanks
>> > Tracey Lenek
>> > Tech III - Anatomic Pathology
>> > Calgary Laboratory Services
>> > 403-770-3588
>> >

Re: [Histonet] Histo humor :-)

[Joe Nocito]

is a casual histo tech like a  casual end table? What is it the rest of the 
time?


Oh, yeah, we not only have a great cents of humor, we're real cut ups to.

JTT
- Original Message - 
From: "Dianne Holmes" <[EMAIL PROTECTED]>

To: "Histonet" 
Sent: Tuesday, December 02, 2008 10:44 AM
Subject: [Histonet] Histo humor :-)


Not only do I get valuable info from this group but histologists have a 
GREAT sense of humor in common !!

I loved the 'casual histo tech' volley!!


Individuals who have received this information in error or are not 
authorized to receive it must promptly return or dispose of the information 
and notify the sender. Those individuals are hereby notified that they are 
strictly prohibited from reviewing, forwarding, printing, copying, 
distributing or using this information in any way.




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Expiration date checks

[CLAIRE ANN KENTLER]

 
Hi All,

Whilst we're on the subject, we're currently trying to troubleshoot an
ELISA assay...  This may be a silly question, but does anyone know if
(or when) Sodium Bicarbonate loses its buffering capacity?  (I.e. does
it expire?)

Thanks,
Claire


***
Claire Kentler
Bachelor of Animal Science (Hons)
Postgraduate Student
Department of Agricultural Sciences
La Trobe University
Phone: 9479 1048
E-mail: [EMAIL PROTECTED]
 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Rande
Kline
Sent: Wednesday, 3 December 2008 2:18 AM
To: Weber, Susan (VHACLE)
Cc: histonet
Subject: Re: [Histonet] Expiration date checks

Patty,

Chemicals really don't last forever.  Changes take place everytime you
open the bottle and some just from age even unopened.  Salts used in
buffers are hygroscopic.  They may appear to look okay but they may not
be for your application.  How long you can use a chemical is dependent
on it's application.  I think everyone has experienced a solution not
working even though you have followed the correct procedure and a repeat
has the same outcome.

I have wrote a couple of articles a few years back that appeared in
Laboratory Medicine Q&A and Microscopy Today on expiration dating of
chemicals.  The article in Microscopy Today is a little more indepth. I
would not mind faxing you a copy.  You may want to dig into   more than
the
dot system.  I would also suggest disposing chemicals that you may not
know the actual age of, if they exist.


On Tue, Dec 2, 2008 at 8:48 AM, Weber, Susan (VHACLE)
<[EMAIL PROTECTED]>wrote:

> Color coding with sticker "dots" helps a bit. I designate a color for 
> each year (5 yrs/5 colors max) On the top of that years color I put a 
> date (usually 6/08 whatever) and make them easier to spot. (Pardon the

> pun!)You can find color dots in the office supply catalog, Office Max 
> (or similar) or the craft stores. Happy Holidays To All!
>
> Sue Weber
>  -Original Message-
> From: [EMAIL PROTECTED]
> [mailto:[EMAIL PROTECTED] On Behalf Of Patti 
> Loykasek
> Sent: Monday, December 01, 2008 6:49 PM
> To: histonet
> Subject: [Histonet] Expiration date checks
>
>  Hi all. I am hoping for some clever ideas on the best method to check

> for expired reagents. We all know that we shouldn't use them, I'm sure

> we all actively monitor this (right)! I must admit we occasionally 
> have expired reagents - a stain or buffer we haven't used in a while 
> is in a cupboard or fridge & it has expired. What do others do to 
> monitor & prevent this from happening? We have a weekly safety check 
> that spot checks a certain # of reagents for expiration, but we 
> probably have thousands of reagents & don't check every single bottle 
> every week. Thanks for the input.
>
> Patti Loykasek
>
>
>
> This e-mail message, including any attachments, is for the sole use of

> the intended recipients and may contain privileged information. Any 
> unauthorized review, use, disclosure or distribution is prohibited. If

> you are not the intended recipient, please contact the sender by 
> e-mail and destroy all copies of the original message, or you may call

> PhenoPath Laboratories, Seattle, WA U.S.A.
> at (206) 374-9000.
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--
Rande Kline, HT (ASCP)
Histopathology Lab Concepts
P-609-744-0803
F-609-939-0270
[EMAIL PROTECTED]
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Large Slide Boxes

[Jack Ratliff]

Pamela,
 
I get my boxes (wooden - holds 100 slides) from Brain Research Labs and they 
cost around $33.00 per box. The website is http://www.brainresearchlab.com/
 
Jack
 
> Date: Tue, 2 Dec 2008 21:40:41 +> From: [EMAIL PROTECTED]> To: 
> histonet@lists.utsouthwestern.edu> Subject: [Histonet] Large Slide Boxes> > > 
> > Good Afternoon All, > > > > I am looking to purchase some 3 inch by 2 inch 
> (3X2) slide boxes for large slides.  We do some larger specimens and it seems 
> these have become almost impossible to find.  I have tried several sources 
> and no one had them or knew where I could get them at this time.  I have 
> tried myneurolab and several other speciality vendors also to no avail.  HELP 
> > > > > Pam Marcum> ___> Histonet 
> mailing list> Histonet@lists.utsouthwestern.edu> 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_
Send e-mail anywhere. No map, no compass.
http://windowslive.com/Explore/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_anywhere_122008___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Neurofilament Question

[Jim Manavis]

Margaret

Get on to the Covance site as they have the Strenberger Neurofilaments.

Cheers

Jim

Jim Manavis
Laboratory Manager
Hanson Institute
Centre for Neurological Diseases
IMVS, Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668 / 0401120697
FAX: 61-08-8222 3392
email: [EMAIL PROTECTED]
Disclaimer: Not this little black duck! 


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Peggy Bisher
Sent: Wednesday, 3 December 2008 7:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Neurofilament Question

I have a student who asked if I could post this question to the Histo-list.
I told her how all of you seem to be very helpful and perhaps some of you
know the answer to her question:



Dear all,


I am looking for a good "catch-all" antibody for neuronal intermediate
filaments (NF-L, NF-M and NF-H). Since they're generally expressed
together in each neurofilament I'm not interested in any one of them
specifically, but want to avoid using a cocktail of antibodies, as
this could get expensive. Basically, I just want to show that a subset
of axons contains neurofilaments with a reliable antibody, but the
particular protein or phosphorylation state doesn't matter. Any
suggestions you could offer would be incredibly helpful!

Sincerely, Eve Schneider


Thanks,


Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
[EMAIL PROTECTED]



 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Cleaved Caspase-3

[Jim Manavis]

Tumour tissue, I use CNS Lymphoma.

Cheers

Jim 

Jim Manavis
Laboratory Manager
Hanson Institute
Centre for Neurological Diseases
IMVS, Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668 / 0401120697
FAX: 61-08-8222 3392
email: [EMAIL PROTECTED]
Disclaimer: Not this little black duck! 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Kimberly
Tuttle
Sent: Wednesday, 3 December 2008 1:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cleaved Caspase-3

Can anyone recommend control tissue?

Thank You

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 


 

This e-mail and any accompanying attachments may be privileged,
confidential, contain protected health information about an identified
patient or be otherwise protected from disclosure. State and federal law
protect the confidentiality of this information. If the reader of this
message is not the intended recipient; you are prohibited from using,
disclosing, reproducing or distributing this information; you should
immediately notify the sender by telephone or e-mail and delete this e-mail.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Large Slide Boxes

[Pamela Marcum]

Good Afternoon All, 



I am looking to purchase some 3 inch by 2 inch (3X2) slide boxes for large 
slides.  We do some larger specimens and it seems these have become almost 
impossible to find.  I have tried several sources and no one had them or knew 
where I could get them at this time.  I have tried myneurolab and several other 
speciality vendors also to no avail.  HELP 



Pam Marcum
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Neurofilament Question

[Kathleen Roberts]

I have not used this one personally,but I have used antibodies against 
individual neurofilaments from this same company (before they were taken 
over by Covance), and they were excellent.  If I'm reading this right, 
it IS a cocktail.  Still pricey, but it might be worth a try.  Maybe you 
can get a sample before you buy?  I don't know if Covance does that, but 
it can't hurt to ask.


https://store.crpinc.com/datasheet.aspx?UpProd=&catalogno=SMI-312R

Good luck,
Kathleen Roberts
Dept of Pharmacology & Toxicology
Rutgers University

Peggy Bisher wrote:


I have a student who asked if I could post this question to the Histo-list.
I told her how all of you seem to be very helpful and perhaps some of you
know the answer to her question:



Dear all,


I am looking for a good "catch-all" antibody for neuronal intermediate
filaments (NF-L, NF-M and NF-H). Since they're generally expressed
together in each neurofilament I'm not interested in any one of them
specifically, but want to avoid using a cocktail of antibodies, as
this could get expensive. Basically, I just want to show that a subset
of axons contains neurofilaments with a reliable antibody, but the
particular protein or phosphorylation state doesn't matter. Any
suggestions you could offer would be incredibly helpful!

Sincerely, Eve Schneider


Thanks,


Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
[EMAIL PROTECTED]






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Neurofilament Question

[Peggy Bisher]

I have a student who asked if I could post this question to the Histo-list.
I told her how all of you seem to be very helpful and perhaps some of you
know the answer to her question:



Dear all,


I am looking for a good "catch-all" antibody for neuronal intermediate
filaments (NF-L, NF-M and NF-H). Since they're generally expressed
together in each neurofilament I'm not interested in any one of them
specifically, but want to avoid using a cocktail of antibodies, as
this could get expensive. Basically, I just want to show that a subset
of axons contains neurofilaments with a reliable antibody, but the
particular protein or phosphorylation state doesn't matter. Any
suggestions you could offer would be incredibly helpful!

Sincerely, Eve Schneider


Thanks,


Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
[EMAIL PROTECTED]



 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ventana Benchmark XTs

[renafail]

 
 In the past I have seen techs insert a disclaimer stating   the opinion is 
theirs and theirs alone and does not reflect the opinion of their employer nor 
do they identify their employer. Some  managers may feel  that it reflects  
poorly on the Lab if  questions are posted. In reality  questions allow for the 
exchange of information and opens a dialogue that may benefit many
Rena Fail , retired
 -- Original message from "Histonet Alias" <[EMAIL PROTECTED]>: 
--


> Mr. Della Speranza, I respect what you have done for the histology community
> and we have had pleasant conversations in the past but you can not speak for
> the Histonet members. You act as if I am hiding my identity to create havoc
> and disruption. I have not done this nor will I do this. I have been on
> histonet for many years and I will continue to do so. As I explained in my
> previous posts my current employer does not want any opinions, help, or
> questions associated with the laboratory. That is the only reason that I
> have chosen to remain anonymous. Would it make anyone feel better if I made
> up a fake name and lab as my signature?  I will continue to post and speak
> freely without breaking any rules of this listserver. If I respond or ask a
> question, those who find my years of experience not "credible" then
> I apologize.
> 
> On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie wrote:
> 
> > I'm afraid your opinion will not be credible as long as you insist on
> > remaining anonymous. It has nothing to do with whether you like or dislike a
> > particular product or instrument.  Histonet members appreciate the
> > responsibility that comes with posting one's views or knowledge by
> > identifying themselves accordingly. In my opinion anyone who insists on
> > remaining anonymous should not be permitted to participate on the list.
> >
> >
> > Vinnie Della Speranza
> > Manager for Anatomic Pathology Services
> > Medical University of South Carolina
> > 165 Ashley Avenue  Suite 309
> > Charleston, South Carolina 29425
> > Tel: (843) 792-6353
> > Fax: (843) 792-8974
> >
> >
> > -Original Message-
> > From: [EMAIL PROTECTED] [mailto:
> > [EMAIL PROTECTED] On Behalf Of Histonet Alias
> > Sent: Saturday, November 29, 2008 11:03 AM
> > To: Mark Tarango
> > Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
> > Burton, Lynn; Joanna Bartczak-McKay
> > Subject: Re: [Histonet] Ventana Benchmark XTs
> >
> > Sorry I don't work for Ventana and I have no beef with them. It
> > may disappoint some that I have not had a bad experience. So my opinion
> > does
> > not count because I have had a different experience with my XT than you? I
> > use the name of The Unknown HT so I can express my opinions freely without
> > my employer receiving phone calls because certain people don't like my
> > opinions.
> >
> > On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango 
> > wrote:
> >
> > > I don't know how much the opinion of "The Unknown HT(ASCP)" counts.  You
> > > aren't with Ventana I hope.
> > >
> > > Mark
> > >
> > > On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias <
> > [EMAIL PROTECTED]>wrote:
> > >
> > >> They are switching to a more reliable manufacturer. They are being
> > >> proactive
> > >> and switching them before they fail down the road. My XT has been a
> > >> workhorse but we know certain peoples opinions about the company on
> > here.
> > >> I
> > >> will take it any day over the rest.
> > >>
> > >> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn 
> > >> >wrote:
> > >>
> > >> > We have had some trouble but they are planning to replace them. We
> > >> received
> > >> > a letter that they had changed vendors for parts and found they need
> > to
> > >> > switch back. They did replace one section earlier when it stopped
> > >> working
> > >> > completely.
> > >> >
> > >> > Lynn Burton
> > >> > Lab Assoc. I
> > >> > Animal Disease Lab
> > >> > Galesburg, Il 61401
> > >> >
> > >> > 
> > >> >
> > >> > From: [EMAIL PROTECTED] on behalf of Tracey
> > >> Lenek
> > >> > Sent: Wed 11/26/2008 10:59 AM
> > >> > To: histonet@lists.utsouthwestern.edu
> > >> > Cc: Martin Trotter; Joanna Bartczak-McKay
> > >> > Subject: [Histonet] Ventana Benchmark XTs
> > >> >
> > >> >
> > >> >
> > >> > Hi,
> > >> >
> > >> > We have had on-going thermal slide pad issues with our XTs since they
> > >> were
> > >> > installed
> > >> > in February. The slide tray assemblies have been replaced not once but
> > >> > twice and we are still having inconsistent
> > >> > results with the temp verifier slides.  Has anyone experienced the
> > same
> > >> > issues with this
> > >> > instrumentation?
> > >> >
> > >> > Thanks
> > >> > Tracey Lenek
> > >> > Tech III - Anatomic Pathology
> > >> > Calgary Laboratory Services
> > >> > 403-770-3588
> > >> >
> > >> > 
> > >> > This message and any attached documents are only for the use of the
> > >> > intended recipient(s), 

Re: [Histonet] Ventana Benchmark XTs

[Rene J Buesa]

This is getting somewhat confusing BUT your initial commentary fully endorsing 
Ventana OVER ANY OTHER, is the one that raised some suspicion because there are 
other autostainers generally accepted as MUCH BETTER than Ventana or put in 
some other way, NO OTHER autostainer has generated so many bad reports.
Either you work for Verntana or not I do not care, but I think that if your 
boss restricts so much somebody with the many years of experience you claim to 
have, either you have a problem with your boss, or you should think in working 
some other place before retiring (after so many years of work in histology).
That is my honest and not anonymous opinion
René J.

--- On Tue, 12/2/08, Mark Tarango <[EMAIL PROTECTED]> wrote:

From: Mark Tarango <[EMAIL PROTECTED]>
Subject: Re: [Histonet] Ventana Benchmark XTs
To: "Histonet Alias" <[EMAIL PROTECTED]>
Cc: "histonet@lists.utsouthwestern.edu" , 
"Tracey Lenek" <[EMAIL PROTECTED]>, "Burton, Lynn" <[EMAIL PROTECTED]>, "Joanna 
Bartczak-McKay" <[EMAIL PROTECTED]>, "Martin Trotter" <[EMAIL PROTECTED]>
Date: Tuesday, December 2, 2008, 3:24 PM

Can't you just post without saying where exactly you work?  I don't
mention
where I'm working these days.  Does your employer own you?  ...or maybe
you're some famous histotech and you get paid for endorsements?  lol

Mark

On Tue, Dec 2, 2008 at 11:19 AM, Histonet Alias
<[EMAIL PROTECTED]>wrote:

> Mr. Della Speranza, I respect what you have done for the histology
> community and we have had pleasant conversations in the past but you can
not
> speak for the Histonet members. You act as if I am hiding my identity to
> create havoc and disruption. I have not done this nor will I do this. I
have
> been on histonet for many years and I will continue to do so. As I
explained
> in my previous posts my current employer does not want any opinions, help,
> or questions associated with the laboratory. That is the only reason that
I
> have chosen to remain anonymous. Would it make anyone feel better if I
made
> up a fake name and lab as my signature?  I will continue to post and speak
> freely without breaking any rules of this listserver. If I respond or ask
a
> question, those who find my years of experience not "credible"
then
> I apologize.
>
>
> On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie
<[EMAIL PROTECTED]>wrote:
>
>> I'm afraid your opinion will not be credible as long as you insist
on
>> remaining anonymous. It has nothing to do with whether you like or
dislike a
>> particular product or instrument.  Histonet members appreciate the
>> responsibility that comes with posting one's views or knowledge by
>> identifying themselves accordingly. In my opinion anyone who insists
on
>> remaining anonymous should not be permitted to participate on the
list.
>>
>>
>> Vinnie Della Speranza
>> Manager for Anatomic Pathology Services
>> Medical University of South Carolina
>> 165 Ashley Avenue  Suite 309
>> Charleston, South Carolina 29425
>> Tel: (843) 792-6353
>> Fax: (843) 792-8974
>>
>>
>> -Original Message-
>> From: [EMAIL PROTECTED] [mailto:
>> [EMAIL PROTECTED] On Behalf Of Histonet Alias
>> Sent: Saturday, November 29, 2008 11:03 AM
>> To: Mark Tarango
>> Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
>> Burton, Lynn; Joanna Bartczak-McKay
>> Subject: Re: [Histonet] Ventana Benchmark XTs
>>
>> Sorry I don't work for Ventana and I have no beef with them. It
>> may disappoint some that I have not had a bad experience. So my
opinion
>> does
>> not count because I have had a different experience with my XT than
you? I
>> use the name of The Unknown HT so I can express my opinions freely
without
>> my employer receiving phone calls because certain people don't
like my
>> opinions.
>>
>> On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango
<[EMAIL PROTECTED]>
>> wrote:
>>
>> > I don't know how much the opinion of "The Unknown
HT(ASCP)" counts.  You
>> > aren't with Ventana I hope.
>> >
>> > Mark
>> >
>> > On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias <
>> [EMAIL PROTECTED]>wrote:
>> >
>> >> They are switching to a more reliable manufacturer. They are
being
>> >> proactive
>> >> and switching them before they fail down the road. My XT has
been a
>> >> workhorse but we know certain peoples opinions about the
company on
>> here.
>> >> I
>> >> will take it any day over the rest.
>> >>
>> >> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn <
>> [EMAIL PROTECTED]
>> >> >wrote:
>> >>
>> >> > We have had some trouble but they are planning to
replace them. We
>> >> received
>> >> > a letter that they had changed vendors for parts and
found they need
>> to
>> >> > switch back. They did replace one section earlier when
it stopped
>> >> working
>> >> > completely.
>> >> >
>> >> > Lynn Burton
>> >> > Lab Assoc. I
>> >> > Animal Disease Lab
>> >> > Galesburg, Il 61401
>> >> >
>> >> > 
>> >> >
>> >> > From: [EMAIL PROTECTED] on
behalf of Tracey
>> >> Lenek
>> >> > Sent: Wed 11/26/2008 

Re: [Histonet] Ventana Benchmark XTs

[Mark Tarango]

Can't you just post without saying where exactly you work?  I don't mention
where I'm working these days.  Does your employer own you?  ...or maybe
you're some famous histotech and you get paid for endorsements?  lol

Mark

On Tue, Dec 2, 2008 at 11:19 AM, Histonet Alias <[EMAIL PROTECTED]>wrote:

> Mr. Della Speranza, I respect what you have done for the histology
> community and we have had pleasant conversations in the past but you can not
> speak for the Histonet members. You act as if I am hiding my identity to
> create havoc and disruption. I have not done this nor will I do this. I have
> been on histonet for many years and I will continue to do so. As I explained
> in my previous posts my current employer does not want any opinions, help,
> or questions associated with the laboratory. That is the only reason that I
> have chosen to remain anonymous. Would it make anyone feel better if I made
> up a fake name and lab as my signature?  I will continue to post and speak
> freely without breaking any rules of this listserver. If I respond or ask a
> question, those who find my years of experience not "credible" then
> I apologize.
>
>
> On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie <[EMAIL 
> PROTECTED]>wrote:
>
>> I'm afraid your opinion will not be credible as long as you insist on
>> remaining anonymous. It has nothing to do with whether you like or dislike a
>> particular product or instrument.  Histonet members appreciate the
>> responsibility that comes with posting one's views or knowledge by
>> identifying themselves accordingly. In my opinion anyone who insists on
>> remaining anonymous should not be permitted to participate on the list.
>>
>>
>> Vinnie Della Speranza
>> Manager for Anatomic Pathology Services
>> Medical University of South Carolina
>> 165 Ashley Avenue  Suite 309
>> Charleston, South Carolina 29425
>> Tel: (843) 792-6353
>> Fax: (843) 792-8974
>>
>>
>> -Original Message-
>> From: [EMAIL PROTECTED] [mailto:
>> [EMAIL PROTECTED] On Behalf Of Histonet Alias
>> Sent: Saturday, November 29, 2008 11:03 AM
>> To: Mark Tarango
>> Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
>> Burton, Lynn; Joanna Bartczak-McKay
>> Subject: Re: [Histonet] Ventana Benchmark XTs
>>
>> Sorry I don't work for Ventana and I have no beef with them. It
>> may disappoint some that I have not had a bad experience. So my opinion
>> does
>> not count because I have had a different experience with my XT than you? I
>> use the name of The Unknown HT so I can express my opinions freely without
>> my employer receiving phone calls because certain people don't like my
>> opinions.
>>
>> On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango <[EMAIL PROTECTED]>
>> wrote:
>>
>> > I don't know how much the opinion of "The Unknown HT(ASCP)" counts.  You
>> > aren't with Ventana I hope.
>> >
>> > Mark
>> >
>> > On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias <
>> [EMAIL PROTECTED]>wrote:
>> >
>> >> They are switching to a more reliable manufacturer. They are being
>> >> proactive
>> >> and switching them before they fail down the road. My XT has been a
>> >> workhorse but we know certain peoples opinions about the company on
>> here.
>> >> I
>> >> will take it any day over the rest.
>> >>
>> >> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn <
>> [EMAIL PROTECTED]
>> >> >wrote:
>> >>
>> >> > We have had some trouble but they are planning to replace them. We
>> >> received
>> >> > a letter that they had changed vendors for parts and found they need
>> to
>> >> > switch back. They did replace one section earlier when it stopped
>> >> working
>> >> > completely.
>> >> >
>> >> > Lynn Burton
>> >> > Lab Assoc. I
>> >> > Animal Disease Lab
>> >> > Galesburg, Il 61401
>> >> >
>> >> > 
>> >> >
>> >> > From: [EMAIL PROTECTED] on behalf of Tracey
>> >> Lenek
>> >> > Sent: Wed 11/26/2008 10:59 AM
>> >> > To: histonet@lists.utsouthwestern.edu
>> >> > Cc: Martin Trotter; Joanna Bartczak-McKay
>> >> > Subject: [Histonet] Ventana Benchmark XTs
>> >> >
>> >> >
>> >> >
>> >> > Hi,
>> >> >
>> >> > We have had on-going thermal slide pad issues with our XTs since they
>> >> were
>> >> > installed
>> >> > in February. The slide tray assemblies have been replaced not once
>> but
>> >> > twice and we are still having inconsistent
>> >> > results with the temp verifier slides.  Has anyone experienced the
>> same
>> >> > issues with this
>> >> > instrumentation?
>> >> >
>> >> > Thanks
>> >> > Tracey Lenek
>> >> > Tech III - Anatomic Pathology
>> >> > Calgary Laboratory Services
>> >> > 403-770-3588
>> >> >
>> >> > 
>> >> > This message and any attached documents are only for the use of the
>> >> > intended recipient(s), are confidential and may contain privileged
>> >> > information. Any unauthorized review, use, retransmission, or other
>> >> > disclosure is strictly prohibited. If you have received this message
>> in
>> >> > error, please notify the

[Histonet] Fontana Masson

[Kalleberg, Kristopher]

All,
 
Does anyone have a reference for me to read up on the chemistry involved
in Fontana Masson staining of melanin?  I am having a hard time finding
in depth information on exactly how FM stain stains the melanosmes and
the chemistry and reactions involved.  Any information, papers, or
references would be greatly appreciated.  Thank you in advance.
 
Kris Kalleberg
Research Scientist
Unilever R&D
40 Merritt Blvd.
Trumbull, CT 06611
(203) 381-5765
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Expiration date checks

[Histonet Alias]

I actually have an excel spread sheet of expiration dates. I also place
those dates into the outlook calender and I receive a notification a week
before the expiration.

On Mon, Dec 1, 2008 at 6:49 PM, Patti Loykasek <[EMAIL PROTECTED]>wrote:

> Hi all. I am hoping for some clever ideas on the best method to check for
> expired reagents. We all know that we shouldn't use them, I'm sure we all
> actively monitor this (right)! I must admit we occasionally have expired
> reagents - a stain or buffer we haven't used in a while is in a cupboard or
> fridge & it has expired. What do others do to monitor & prevent this from
> happening? We have a weekly safety check that spot checks a certain # of
> reagents for expiration, but we probably have thousands of reagents & don't
> check every single bottle every week. Thanks for the input.
>
> Patti Loykasek
>
>
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipients and may contain privileged information. Any
> unauthorized
> review, use, disclosure or distribution is prohibited. If you are not the
> intended
> recipient, please contact the sender by e-mail and destroy all copies of
> the
> original message, or you may call PhenoPath Laboratories, Seattle, WA
> U.S.A.
> at (206) 374-9000.
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
The Unknown HT(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Best Tissue for H&E Control

[Histonet Alias]

Hi Tanisha,
We use an appendix for our H&E qc.

On Tue, Dec 2, 2008 at 11:33 AM, McKnight, Tanisha <
[EMAIL PROTECTED]> wrote:

> Hello All:
>
> Can I get some of you to share with me, what types of tissue you use to
> assess the quality of your H&E? Or what tissue type do you believe to be
> best for assessing the quality of an H&E?
>
> Thanks,
>
> Tanisha Neely, HT(ASCP)
> AP-Histology/Specimen Management
> Covance CLS, Indianapolis
>
>
>
>
> -
> Confidentiality Notice: This e-mail transmission
> may contain confidential or legally privileged
> information that is intended only for the individual
> or entity named in the e-mail address. If you are not
> the intended recipient, you are hereby notified that
> any disclosure, copying, distribution, or reliance
> upon the contents of this e-mail is strictly prohibited.
>
> If you have received this e-mail transmission in error,
> please reply to the sender, so that we can arrange
> for proper delivery, and then please delete the message
> from your inbox. Thank you.
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
The Unknown HT(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ventana Benchmark XTs

[Histonet Alias]

Mr. Della Speranza, I respect what you have done for the histology community
and we have had pleasant conversations in the past but you can not speak for
the Histonet members. You act as if I am hiding my identity to create havoc
and disruption. I have not done this nor will I do this. I have been on
histonet for many years and I will continue to do so. As I explained in my
previous posts my current employer does not want any opinions, help, or
questions associated with the laboratory. That is the only reason that I
have chosen to remain anonymous. Would it make anyone feel better if I made
up a fake name and lab as my signature?  I will continue to post and speak
freely without breaking any rules of this listserver. If I respond or ask a
question, those who find my years of experience not "credible" then
I apologize.

On Mon, Dec 1, 2008 at 11:21 AM, Della Speranza, Vinnie <[EMAIL 
PROTECTED]>wrote:

> I'm afraid your opinion will not be credible as long as you insist on
> remaining anonymous. It has nothing to do with whether you like or dislike a
> particular product or instrument.  Histonet members appreciate the
> responsibility that comes with posting one's views or knowledge by
> identifying themselves accordingly. In my opinion anyone who insists on
> remaining anonymous should not be permitted to participate on the list.
>
>
> Vinnie Della Speranza
> Manager for Anatomic Pathology Services
> Medical University of South Carolina
> 165 Ashley Avenue  Suite 309
> Charleston, South Carolina 29425
> Tel: (843) 792-6353
> Fax: (843) 792-8974
>
>
> -Original Message-
> From: [EMAIL PROTECTED] [mailto:
> [EMAIL PROTECTED] On Behalf Of Histonet Alias
> Sent: Saturday, November 29, 2008 11:03 AM
> To: Mark Tarango
> Cc: histonet@lists.utsouthwestern.edu; Martin Trotter; Tracey Lenek;
> Burton, Lynn; Joanna Bartczak-McKay
> Subject: Re: [Histonet] Ventana Benchmark XTs
>
> Sorry I don't work for Ventana and I have no beef with them. It
> may disappoint some that I have not had a bad experience. So my opinion
> does
> not count because I have had a different experience with my XT than you? I
> use the name of The Unknown HT so I can express my opinions freely without
> my employer receiving phone calls because certain people don't like my
> opinions.
>
> On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango <[EMAIL PROTECTED]>
> wrote:
>
> > I don't know how much the opinion of "The Unknown HT(ASCP)" counts.  You
> > aren't with Ventana I hope.
> >
> > Mark
> >
> > On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias <
> [EMAIL PROTECTED]>wrote:
> >
> >> They are switching to a more reliable manufacturer. They are being
> >> proactive
> >> and switching them before they fail down the road. My XT has been a
> >> workhorse but we know certain peoples opinions about the company on
> here.
> >> I
> >> will take it any day over the rest.
> >>
> >> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn <[EMAIL PROTECTED]
> >> >wrote:
> >>
> >> > We have had some trouble but they are planning to replace them. We
> >> received
> >> > a letter that they had changed vendors for parts and found they need
> to
> >> > switch back. They did replace one section earlier when it stopped
> >> working
> >> > completely.
> >> >
> >> > Lynn Burton
> >> > Lab Assoc. I
> >> > Animal Disease Lab
> >> > Galesburg, Il 61401
> >> >
> >> > 
> >> >
> >> > From: [EMAIL PROTECTED] on behalf of Tracey
> >> Lenek
> >> > Sent: Wed 11/26/2008 10:59 AM
> >> > To: histonet@lists.utsouthwestern.edu
> >> > Cc: Martin Trotter; Joanna Bartczak-McKay
> >> > Subject: [Histonet] Ventana Benchmark XTs
> >> >
> >> >
> >> >
> >> > Hi,
> >> >
> >> > We have had on-going thermal slide pad issues with our XTs since they
> >> were
> >> > installed
> >> > in February. The slide tray assemblies have been replaced not once but
> >> > twice and we are still having inconsistent
> >> > results with the temp verifier slides.  Has anyone experienced the
> same
> >> > issues with this
> >> > instrumentation?
> >> >
> >> > Thanks
> >> > Tracey Lenek
> >> > Tech III - Anatomic Pathology
> >> > Calgary Laboratory Services
> >> > 403-770-3588
> >> >
> >> > 
> >> > This message and any attached documents are only for the use of the
> >> > intended recipient(s), are confidential and may contain privileged
> >> > information. Any unauthorized review, use, retransmission, or other
> >> > disclosure is strictly prohibited. If you have received this message
> in
> >> > error, please notify the sender immediately, and then delete the
> >> original
> >> > message. Thank you.
> >> > ___
> >> > Histonet mailing list
> >> > Histonet@lists.utsouthwestern.edu
> >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >> >
> >> >
> >> > ___
> >> > Histonet mailing list
> >> > Histonet@lists.utsouthwestern.edu
> >> > http://lists.utsouthweste

[Histonet] Control tissue

[Webb, Dorothy L]

The best control is healthy skin with the connective tissue in place but a 
tonsil is also great for checking how well your lymph tissue is staining.  
Tonsil should be cut thinner just like the lymph nodes so you should use the 
tonsil when staining lymph nodes.  Make sure the tonsil has connective tissue 
in it so you can see the eosin staining in the collagen, blood vessels, 
cytoplasm, and RBC's.  Another good tissue is cervix with the glands.  The 
glandular area gives you the nice hematoxylin staining and the muscle and 
collagen fibers give you a nice contrast on the eosin staining plus you can 
always get cervix. 

I would stay away from the appendix since there is usually a lot of necrosis 
and the placenta is a little to bloody so the eosin is always some what 
overwhelming for a good evaluation. 
 
This is information I was given when posing the question years ago!!

Dorothy Webb, HT
Regions Histology Technical Supervisor
651-254-2962

This e-mail and any files transmitted with it are confidential and are intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient or the individual responsible for delivering 
the e-mail to the intended recipient, please be advised that you have received 
this e-mail in error and that any use, dissemination, forwarding, printing, or 
copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the 
HealthPartners Support Center by telephone at (952) 967-6600. You will be 
reimbursed for reasonable costs incurred in notifying us.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Histonet Digest, Vol 61, Issue 2 H&E CONTROL TISSUE

[Kay, Karen]

Hello Tanisha,
We feel that no one tissue is suitable on its own, therefore we create a
multi-tissue control block for our H&E stains. The tissues included are
liver, skin, appendix, and placenta. This enables us to assess the stain
from a number of different perspectives according to the tissue types
included. We use small portions of each type, embedded into a
rectangular shaped mold.
Hope this helps you
Karen J Kay, MLT
Pathology Supervisor
Chinook Health Laboratory
Chinook Regional Hospital
960 - 19 st South
Lethbridge, Alberta, Canada
T1J 1W5
(403) 388 - 6061 (Phone)
(403) 388 - 6067 (Fax)




Message: 8
Date: Tue, 2 Dec 2008 11:33:22 -0500
From: "McKnight, Tanisha" <[EMAIL PROTECTED]>
Subject: [Histonet] Best Tissue for H&E Control
To: histonet@lists.utsouthwestern.edu
Message-ID:

<[EMAIL PROTECTED]>
Content-Type: text/plain; charset="us-ascii"

Hello All:
 
Can I get some of you to share with me, what types of tissue you use to
assess the quality of your H&E? Or what tissue type do you believe to be
best for assessing the quality of an H&E?
 
Thanks,
 
Tanisha Neely, HT(ASCP)
AP-Histology/Specimen Management
Covance CLS, Indianapolis


This communication is intended for the use of the recipient to which it is 
addressed, and may contain confidential, personal and or privileged 
information.  Please contact us immediately if you are not the intended 
recipient.  Do not copy, distribute or take action relying on it. Any 
communication received in error, or subsequent reply, should be deleted or 
destroyed.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Halt

[Christine Bark]

You can get it from Poly Scientific www.polyrnd.com

Christine Bark HT(ASCP) CM
Lead Histotech, Pathology
Saddleback Memorial Medical Center
949-452-3548
[EMAIL PROTECTED]
 

Hello all, 

Can anyone tell me where I could buy Halt. It is used to get wrinkle
free sections on the floatation bath.

Thanks 
Jenny

__
Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure or 
distribution 
is prohibited. If you are not the intended recipient, please contact the sender 
by reply e-mail and destroy all copies of the original message.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Update - Rescheduling of previously cancelled LCM Webinar

[Chu, Shirley]

My apologies to all, but the link that I provided below for registration
is incorrect, please use this link to register for this webinar:
http://www.moleculardevices.com/pages/lcm_webinar_12.2008.html




From: Chu, Shirley 
Sent: Tuesday, December 02, 2008 9:03 AM
To: 'histonet@lists.utsouthwestern.edu'
Cc: 'NYSHistotech'
Subject: Rescheduling of previously cancelled LCM Webinar


The cancelled webinar that was scheduled for Nov 24th has been
rescheduled for Friday, Dec 5th at 10AM PST or 1PM EST.  The speaker for
this webinar will be Charmain Pietersen, PhD., Laboratory for Structural
and Molecular Neuroscience, McLean Hospital.  Her presentation is
titled:  "Gene expression analysis in homogenous single cell populations
in the superior temporal gyrus in postmortem brains from subjects with
schizophrenia".   This webinar is free to all registrants.  For further
information and/or to register for this webinar, please connect to the
following website:
http://www.moleculardevices.com/pages/lcm_webinar_11.2008.html.  Please
note anyone that had previously registered for this webinar do not need
to register again.  Please use the log in information that was forwarded
to you upon registering to log into the webinar this friday. 
 
 

Shirley Chu

Application Scientist, Arcturus LCM Products

Molecular Devices

(now a part of MDS Analytical Technologies)

408-747-3765 | www.moleculardevices.com

 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Rescheduling of previously cancelled LCM Webinar

[Chu, Shirley]

The cancelled webinar that was scheduled for Nov 24th has been
rescheduled for Friday, Dec 5th at 10AM PST or 1PM EST.  The speaker for
this webinar will be Charmain Pietersen, PhD., Laboratory for Structural
and Molecular Neuroscience, McLean Hospital.  Her presentation is
titled:  "Gene expression analysis in homogenous single cell populations
in the superior temporal gyrus in postmortem brains from subjects with
schizophrenia".   This webinar is free to all registrants.  For further
information and/or to register for this webinar, please connect to the
following website:
http://www.moleculardevices.com/pages/lcm_webinar_11.2008.html.  Please
note anyone that had previously registered for this webinar do not need
to register again.  Please use the log in information that was forwarded
to you upon registering to log into the webinar this friday. 
 
 

Shirley Chu

Application Scientist, Arcturus LCM Products

Molecular Devices

(now a part of MDS Analytical Technologies)

408-747-3765 | www.moleculardevices.com

 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Question RE: HQIP Result

[Burnette, Phyllis]

When responding about the primary fixative being alcohol, I was also 
referencing HQIP Participant Summary from 2007 which states "Cell shrinkage and 
nuclear bubbling, artifacts induced by aggressive processing schedules with 
resulting primary fixation largely in alcohols". (It's weird, I do actually 
read these things!) I know for us this was endometrial tissue/ small biopsies 
that was evaluated and the infiltration of fixative was not long enough (even 
core breast bx's are now recommended to be included in the no less than 6 hour 
fixative time frame for Her2 testing). So looking at your fixation processing 
times may be of help. This is just what has helped the quality of our tissue 
sections in our lab as we tend to rush these small biopsies through.  Fyi,  
Phyllis

I do have a question though, now that I'm on the subject of the HQIP surveys.  
Did anyone read the comment on the AFB staining from HQIP-B 2007 final critique 
pg. 18??  I keep waiting for some further feed back about IHC for AFB.  They 
mention IHC sensitivity is 74%-100% whereas the traditional special is only 
36%-44% (they made it sound like labs are doing this IHC routinely).  Their 
reference is to the Indian Journal of Tuberculosis.  I have called all of our 
major vendors and they are not aware of a monoclonal antibody to 38k da protein 
of mycobacterium tuberculosis complex.  I just don't understand why CAP would 
recommend something that I cannot find readily available on the market. Any 
comments??

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang
Sent: Tuesday, December 02, 2008 10:57 AM
To: [EMAIL PROTECTED]
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Question RE: HQIP Result 

Schaundra,
In the following publication Fox describes bubbles that occur in cultered cells 
after 5-30 min after the addition of formaldehyd solution.
Fox Cecil H ua., Formaldehyd Fixation; J. of. Histochemistry and Cytochemistry 
33(8):845-853, 1985

So bubbles could be a sign of very short fixation.
Gudrun Lang

-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Schaundra Walton
Gesendet: Montag, 01. Dezember 2008 18:58
An: Histonet
Betreff: [Histonet] Question RE: HQIP Result 

We just got our HQIP results and they made a comment regarding the breast 
tissue H&E slide we submitted.  Can someone please tell me what nuclear 
bubbling artifact is and what causes it?  
 
Thanks!
Schaundra Walton BS, HTL (ASCP)
Histology Supervisor
Swedish American Hospital
Rockford, IL


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this communication is intended to be confidential to the 
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is 
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the 
intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized 
in any manner, by any other person(s). Should
this communication be received in error, please notify the sender immediately 
either by response e-mail or by phone,
and permanently delete the original e-mail, attachment(s), and any copies.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histo humor :-)

[Dianne Holmes]

Not only do I get valuable info from this group but histologists have a GREAT 
sense of humor in common !!  
I loved the 'casual histo tech' volley!!


Individuals who have received this information in error or are not authorized 
to receive it must promptly return or dispose of the information and notify the 
sender. Those individuals are hereby notified that they are strictly prohibited 
from reviewing, forwarding, printing, copying, distributing or using this 
information in any way.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: GMA embedded section attachment to slides

[fernandom . munoz]

Hi James:
We work with technovit 7200 VLC and use Silicoup (kulzer) as  
pretreatment for the glass slides. The blocks must be completely dry  
(I leave one day drying in the lab without working on it).

Fernando Muñoz

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Best Tissue for H&E Control

[McKnight, Tanisha]

Hello All:
 
Can I get some of you to share with me, what types of tissue you use to
assess the quality of your H&E? Or what tissue type do you believe to be
best for assessing the quality of an H&E?
 
Thanks,
 
Tanisha Neely, HT(ASCP)
AP-Histology/Specimen Management
Covance CLS, Indianapolis




-
Confidentiality Notice: This e-mail transmission 
may contain confidential or legally privileged 
information that is intended only for the individual 
or entity named in the e-mail address. If you are not 
the intended recipient, you are hereby notified that 
any disclosure, copying, distribution, or reliance 
upon the contents of this e-mail is strictly prohibited. 

If you have received this e-mail transmission in error, 
please reply to the sender, so that we can arrange 
for proper delivery, and then please delete the message 
from your inbox. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Casual Histo tech postition in Port Huron Michigan

[Bonner, Janet]

Is this Part-time?
 
Janet L. Bonner, HTL (ASCP)
Pathology Laboratory
Florida Hospital Winter Park
[EMAIL PROTECTED]  
407-646-7559



From: [EMAIL PROTECTED] on behalf of sheila adey
Sent: Tue 12/2/2008 10:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Casual Histo tech postition in Port Huron Michigan




Hi All,

We have a casual histo tech position posted at our facility. If interested 
please visit the Port Huron Hospital website for further information.
Sheila Adey HT MLT Port Huron Hospital Michigan
_

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



===
The information contained in this message may be privileged and/or confidential
and protected from disclosure.  If the reader of this message is not the 
intended 
recipient or an employee or agent responsible for delivering this message to 
the 
intended recipient, you are hereby notified that any dissemination, 
distribution 
or copying of this communication is strictly prohibited.  If you have received 
this
communication in error, please notify the sender immediately by replying to 
this 
message and deleting the material from any computer.
===
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

AW: [Histonet] Question RE: HQIP Result

[Gudrun Lang]

Schaundra,
In the following publication Fox describes bubbles that occur in cultered
cells after 5-30 min after the addition of formaldehyd solution.
Fox Cecil H ua., Formaldehyd Fixation; J. of. Histochemistry and
Cytochemistry 33(8):845-853, 1985

So bubbles could be a sign of very short fixation.
Gudrun Lang

-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Schaundra
Walton
Gesendet: Montag, 01. Dezember 2008 18:58
An: Histonet
Betreff: [Histonet] Question RE: HQIP Result 

We just got our HQIP results and they made a comment regarding the breast
tissue H&E slide we submitted.  Can someone please tell me what nuclear
bubbling artifact is and what causes it?  
 
Thanks!
Schaundra Walton BS, HTL (ASCP)
Histology Supervisor
Swedish American Hospital 
Rockford, IL


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cleaved Caspase-3

[anh2006]

Tonsil is great. Also tumor and spleen can work.

--Original Message--
From: Kimberly Tuttle
Sender: [EMAIL PROTECTED]
To: histonet@lists.utsouthwestern.edu
Sent: Dec 2, 2008 10:18 AM
Subject: [Histonet] Cleaved Caspase-3

Can anyone recommend control tissue?

Thank You

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 


 

This e-mail and any accompanying attachments may be privileged, confidential, 
contain protected health information about an identified patient or be 
otherwise protected from disclosure. State and federal law protect the 
confidentiality of this information. If the reader of this message is not the 
intended recipient; you are prohibited from using, disclosing, reproducing or 
distributing this information; you should immediately notify the sender by 
telephone or e-mail and delete this e-mail.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Casual Histotech

[Weems, Joyce]

Just like formal alcohol... We wear our tuxedos... :>)

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Tuesday, December 02, 2008 10:44 AM
To: JD; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Casual Histotech

As opposed to the rest of us...you know, the formal ones. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
[EMAIL PROTECTED]


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of JD
Sent: Tuesday, December 02, 2008 10:42 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Casual Histotech

My thoughts exactly. Casual
histotech?___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Casual Histotech

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

As opposed to the rest of us...you know, the formal ones. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
[EMAIL PROTECTED]


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of JD
Sent: Tuesday, December 02, 2008 10:42 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Casual Histotech

My thoughts exactly. Casual
histotech?___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Casual Histotech

[JD]

My thoughts exactly. Casual 
histotech?___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cleaved Caspase-3

[Larry Woody]

Tumor tissue works well.
 
Larry A. Woody
Seattle, Wa.





From: Kimberly Tuttle <[EMAIL PROTECTED]>
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, December 2, 2008 7:18:09 AM
Subject: [Histonet] Cleaved Caspase-3

Can anyone recommend control tissue?

Thank You

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 




This e-mail and any accompanying attachments may be privileged, confidential, 
contain protected health information about an identified patient or be 
otherwise protected from disclosure. State and federal law protect the 
confidentiality of this information. If the reader of this message is not the 
intended recipient; you are prohibited from using, disclosing, reproducing or 
distributing this information; you should immediately notify the sender by 
telephone or e-mail and delete this e-mail.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Casual Histo tech postition in Port Huron Michigan

[Blazek, Linda]

You work only when you feel like it?  And in jeans and t shirt.


 

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED]
Sent: Tuesday, December 02, 2008 10:17 AM
To: sheila adey; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Casual Histo tech postition in Port Huron Michigan


 Ok , I'll bite. What is a casual histotech?
Rena Fail  -- Original message from sheila adey <[EMAIL 
PROTECTED]>: --


> 
> Hi All,
>  We have a casual histo tech position posted at our facility. If interested 
> please visit the Port Huron Hospital website for further information.
> Sheila Adey HT MLT Port Huron Hospital Michigan
> _
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cleaved Caspase-3

[Kimberly Tuttle]

Can anyone recommend control tissue?

Thank You

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 


 

This e-mail and any accompanying attachments may be privileged, confidential, 
contain protected health information about an identified patient or be 
otherwise protected from disclosure. State and federal law protect the 
confidentiality of this information. If the reader of this message is not the 
intended recipient; you are prohibited from using, disclosing, reproducing or 
distributing this information; you should immediately notify the sender by 
telephone or e-mail and delete this e-mail.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Expiration date checks

[Rande Kline]

Patty,

Chemicals really don't last forever.  Changes take place everytime you open
the bottle and some just from age even unopened.  Salts used in buffers are
hygroscopic.  They may appear to look okay but they may not be for your
application.  How long you can use a chemical is dependent on it's
application.  I think everyone has experienced a solution not working even
though you have followed the correct procedure and a repeat has the same
outcome.

I have wrote a couple of articles a few years back that appeared in
Laboratory Medicine Q&A and Microscopy Today on expiration dating of
chemicals.  The article in Microscopy Today is a little more indepth. I
would not mind faxing you a copy.  You may want to dig into   more than the
dot system.  I would also suggest disposing chemicals that you may not know
the actual age of, if they exist.


On Tue, Dec 2, 2008 at 8:48 AM, Weber, Susan (VHACLE)
<[EMAIL PROTECTED]>wrote:

> Color coding with sticker "dots" helps a bit. I designate a color for
> each year (5 yrs/5 colors max) On the top of that years color I put a
> date (usually 6/08 whatever) and make them easier to spot. (Pardon the
> pun!)You can find color dots in the office supply catalog, Office Max
> (or similar) or the craft stores. Happy Holidays To All!
>
> Sue Weber
>  -Original Message-
> From: [EMAIL PROTECTED]
> [mailto:[EMAIL PROTECTED] On Behalf Of Patti
> Loykasek
> Sent: Monday, December 01, 2008 6:49 PM
> To: histonet
> Subject: [Histonet] Expiration date checks
>
>  Hi all. I am hoping for some clever ideas on the best method to check
> for
> expired reagents. We all know that we shouldn't use them, I'm sure we
> all
> actively monitor this (right)! I must admit we occasionally have expired
> reagents - a stain or buffer we haven't used in a while is in a cupboard
> or
> fridge & it has expired. What do others do to monitor & prevent this
> from
> happening? We have a weekly safety check that spot checks a certain # of
> reagents for expiration, but we probably have thousands of reagents &
> don't
> check every single bottle every week. Thanks for the input.
>
> Patti Loykasek
>
>
>
> This e-mail message, including any attachments, is for the sole use of
> the
> intended recipients and may contain privileged information. Any
> unauthorized
> review, use, disclosure or distribution is prohibited. If you are not
> the intended
> recipient, please contact the sender by e-mail and destroy all copies of
> the
> original message, or you may call PhenoPath Laboratories, Seattle, WA
> U.S.A.
> at (206) 374-9000.
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Rande Kline, HT (ASCP)
Histopathology Lab Concepts
P-609-744-0803
F-609-939-0270
[EMAIL PROTECTED]
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Casual Histo tech postition in Port Huron Michigan

[renafail]

 Ok , I'll bite. What is a casual histotech?
Rena Fail  -- Original message from sheila adey <[EMAIL 
PROTECTED]>: --


> 
> Hi All,
>  We have a casual histo tech position posted at our facility. If interested 
> please visit the Port Huron Hospital website for further information.
> Sheila Adey HT MLT Port Huron Hospital Michigan
> _
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Casual Histo tech postition in Port Huron Michigan

[sheila adey]

Hi All,
 
We have a casual histo tech position posted at our facility. If interested 
please visit the Port Huron Hospital website for further information.
Sheila Adey HT MLT Port Huron Hospital Michigan
_

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] antibody

[MaryAnn Dixon]

Does anyone out there know where I can find an antibody to buy against
Pythium insidiosum?  I can't even find it on an antibody search
engine!

 

MaryAnn Dixon  BS

Biological Scientist

Anatomic Pathology

UF Veterinary Medical Center

(352) 392-2235 Ext. 4517

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] protocols on Ventana

[Angela Bitting]

From what company do you order your PMS2, MSH2 and MSH6 concentrates?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


>>> "Piero Nelva" <[EMAIL PROTECTED]> 12/2/2008 2:52 AM >>>
I've deleted the original post, but to the person who was after Ventana 
protocols for Mismatch Repair proteins, here are ours:

MSH2, 1:50, CC1 Mild, 37, 28
MSH6, 1:4000, CC1 Std, 37, 40 plus amplification kit
MLH1, Vetnana predilute, CC1 Mild,  42, 28 plus amplification kit
PMS2, 1:100, CC1 Mild, 37, 56 plus amplification kit.

I can send you some images of our results off list if you like

Regards

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



IMPORTANT WARNING: The information in this message (and the documents attached 
to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
distribution or any action taken, or omitted to be taken, in reliance on it is 
prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
immediately by replying to this email. Thank you.BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF;NGW:[EMAIL PROTECTED]
N:Bitting;Angela
END:VCARD

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Expiration date checks

[Weber, Susan (VHACLE)]

Color coding with sticker "dots" helps a bit. I designate a color for
each year (5 yrs/5 colors max) On the top of that years color I put a
date (usually 6/08 whatever) and make them easier to spot. (Pardon the
pun!)You can find color dots in the office supply catalog, Office Max
(or similar) or the craft stores. Happy Holidays To All!

Sue Weber
 -Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Patti
Loykasek
Sent: Monday, December 01, 2008 6:49 PM
To: histonet
Subject: [Histonet] Expiration date checks

Hi all. I am hoping for some clever ideas on the best method to check
for
expired reagents. We all know that we shouldn't use them, I'm sure we
all
actively monitor this (right)! I must admit we occasionally have expired
reagents - a stain or buffer we haven't used in a while is in a cupboard
or
fridge & it has expired. What do others do to monitor & prevent this
from
happening? We have a weekly safety check that spot checks a certain # of
reagents for expiration, but we probably have thousands of reagents &
don't
check every single bottle every week. Thanks for the input.

Patti Loykasek



This e-mail message, including any attachments, is for the sole use of
the 
intended recipients and may contain privileged information. Any
unauthorized 
review, use, disclosure or distribution is prohibited. If you are not
the intended 
recipient, please contact the sender by e-mail and destroy all copies of
the 
original message, or you may call PhenoPath Laboratories, Seattle, WA
U.S.A. 
at (206) 374-9000.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Immediate Need for Histotech in Myrtle Beach, SC

[Chris Handrahan]

The #1 Recruiter for Laboratory/Biotech Specialists

Healthcare Scouts is a medical laboratory/biotech recruitment firm that
places professionals in full-time, permanent positions throughout the
United States. As the leading nationally recognized permanent placement
solution for medical laboratory/biotech professionals, Healthcare Scouts
has the following histology opening in Myrtle Beach

 

Day shift 

8-5 M-F

With a brand new urology practice

Full time permanent position 

Looking to hire this week!

 

Referrals

 

With employment opportunities all over the US, we are always looking for
qualified and motivated professionals. Recommend a friend or family
member, and you could be eligible for up to $1,000 through Healthcare
Scouts' referral program. Call us today to learn more!

 

 

 

 

 

 

For immediate consideration please contact 

 

 

Chris Handrahan 

Managing Director of Allied Health

Healthcare Scouts

800-708-0605 office

321-231-5427 cell

[EMAIL PROTECTED]

 

 

 www.healthcarescouts.com

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] (no subject)

[Angelina Subotic]

I am trying to find a full procedure for staining with Schiff reagens and 
naphtol blue black.Does anyone have a protocol they could send me? Any 
information will be greatly appreciated . Best regards 

 

Angelina Subotic
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet