[Histonet] merry christmas
To all histonetters around the world, may you have a safe and happy festive holiday. Best wishes for 2009. Regards Michelle from down south in Africa. Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: michelle.perr...@uct.ac.za ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Understained my slides with hematoxylin
Joe, I would make sure all the mountant is removed. A good soak in xylene and then a rinse in alcohol, if the mountant has not fully disappeared, you will see a white opacity on the section. Rinse in water and then re-stain as usual. The eosin will tend to leach out in the water rinses and the differentiation solution (after the haematoxylin - if you use it). Check the sections after blueing. If it looks too weak, increase the Hx staining time &/or decrease the differentiation step. Counterstain with eosin as usual and you should be right. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Haviland,Joie Sent: Wednesday, 24 December 2008 7:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Understained my slides with hematoxylin Hello: I was doing H&E's and it was pointed out that I had understained the hematoxylin. But the eosin was ok. How does one go back and restain the slides and remove the old stain? Or do you use a differentiating solution? Thanks for your help Happy Holidays Joie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Full Time Permanent Day Shift Histotech Position in Fort Wayne, IN
I work with a tech that you, Melissa, said was getting $1,000 after her placement. After five months, multiple phone calls, and letter writing she only got $500. You wouldn't get back to her. Mark On Mon, Dec 22, 2008 at 7:47 AM, Melissa Phelan wrote: > Hello, > > > > My name is Melissa Phelan and I am the* *Director of Lab/Biotech > recruitment > here at Healthcare Scouts. My company *specializes* in the * > permanent/direct* placement of laboratory professionals like you. We are > currently partnered with numerous labs, and hospitals to recruit for > their *full > time/fully befitted* lab and biotech positions nationwide. I have a > position > in *Fort Wayne, IN*, and it would be a great fit for you and that you > uniquely qualify for. I would like to give you the specifics on this > position, and I would also like to talk with you in more detail on exactly > what you are looking for, so I can help you find the best opportunity for > you. I would also like to give you the opportunity to earn our $1000 > referral bonus, if we place someone that you know in a position, we would > pay you up to $1000 for referring us to that qualified candidate. You can > reach me at any time at 800.708.0605, ext 139 and ask for Melissa. > > > -- > Melissa Phelan > > Laboratory and Biotech > > Phone: 800.708.0605 ext. 139 > > Cell: 407-697-1175 > > UCF KNIGHTS!! > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] charcot-leyden crystals
Can someone tell me the best stain to use for Charcot Leyden crystals? We are looking for them in feces. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 MarshallSlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Understained my slides with hematoxylin
Hello: I was doing H&E's and it was pointed out that I had understained the hematoxylin. But the eosin was ok. How does one go back and restain the slides and remove the old stain? Or do you use a differentiating solution? Thanks for your help Happy Holidays Joie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Myelin stain
Nicole, You can try cresyl echt violet. It preserves RNA/ DNA well. Ambion has a kit, AM1935 that we have used before. For LCM we use it on frozen tissue, though. Good luck I hope this helps! ~ Beth ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Please unsubscribe
Please unsubscribe me from the list. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shurwave microwave
Happy Holiday's Everyone! I was wondering if any of you use a shurwave microprocessor and might happen to have an extra cassett rack i could borrow for a couple of weeks. Our was "accidently" thrown away. also known as tech not paying attention! I am over it! (not really) Any help would be greatly appreciated. Thanks in Advance Amanda LaFlam, HT (ASCP) Pioneer Valley Urology Group _ It’s the same Hotmail®. If by “same” you mean up to 70% faster. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_broad1_122008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Director Position Opportunity
We have an opportunity for an AP Director in Charlotte, NC Director, Carolinas Laboratory Network Located throughout the Charlotte, NC area, Carolinas HealthCare System is a premier, multi-faceted healthcare organization whose facilities make up one of the largest integrated healthcare systems in the United States. A highly visible opportunity exists for a Director in the Anatomic Pathology and Cytology area of Carolinas Laboratory Network to act as a liaison between hospital administration, the department, and the medical staff. Key responsibilities include: administering department policies and procedures; collecting, generating, analyzing, and distributing statistical reports as required; developing a long-range operational plan relative to services, programs, capital, and human resources planning; evaluating and counseling employees relative to performance, conduct, attendance, and adherence to department/hospital rules and regulations; and following up on error correction and establishing corrective action to address identified problems. Qualified candidates must possess a Bachelor's degree, ASCP certification, and a minimum of five years of experience in a medical laboratory with at least two years of supervisory management experience to include personnel, financial, regulatory, and information systems expertise. An advanced degree, preferably in management, or other advanced management training is desirable. For more information about our System and to register online, please visit: www.carolinashealthcare.org/careers or call (704) 444-3041. EOE/AA - This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide drying
We use MM24 from Surgipath. We file our slides within 24 hours. I don't know if it will work in 12 hours, but maybe it's worth trying. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Martin, Erin" 12/22/2008 2:06 PM >>> Hello everyone, I was asked to find out how to dry slides quickly. They are glass coverslipped in an automated coverslipper at the reference lab we use and the our docs want them filed in less than 12 hours from the time they are coverslipped. We have been putting them in a 125 degree C convection oven for a few hours but the slides still get all stuck together in the file. They will not consider film coverslipping. Does anyone else file this quickly? I am grateful for any suggestions! Erin Martin UCSF Dermatopathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] formaldehyd fixation - ph
Dear Gudrun: In my paper on formaldehyde that I sent you some days ago, I mention the work by Helander (1994) in Table 2 where it is shown that formaldehyde fixation is faster at pH7 than at pH4 using radioactive carbon (Section"4", pp 390 of my article). Hope this will answer your question. Happy holidays. René J. --- On Tue, 12/23/08, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] formaldehyd fixation - ph To: histonet@lists.utsouthwestern.edu Date: Tuesday, December 23, 2008, 6:56 AM Hi dear listmembers, Is it generally accepted, that formaldehyd fixation in an acid environment is less effective than in basic? Or in other words: Does formaldehyde bind to proteins in acid solution to a smaller amount than in basic solutions? I have found a publication (Theis, 1944) where the the amount of bound formaldehyde was plotted against pH. And the line increased with the pH. A few years later Gustavson published that Theis' methods of measurement were wrong and also his findings about the reactionpartners of formaldehyde. But I cannot find a newer paper about the effect of pH on formaldehydfixation. Can anyone help me out? Froehliche Weihnachten! Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] formaldehyd fixation - ph
Hi dear listmembers, Is it generally accepted, that formaldehyd fixation in an acid environment is less effective than in basic? Or in other words: Does formaldehyde bind to proteins in acid solution to a smaller amount than in basic solutions? I have found a publication (Theis, 1944) where the the amount of bound formaldehyde was plotted against pH. And the line increased with the pH. A few years later Gustavson published that Theis' methods of measurement were wrong and also his findings about the reactionpartners of formaldehyde. But I cannot find a newer paper about the effect of pH on formaldehydfixation. Can anyone help me out? Froehliche Weihnachten! Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
