RE: [Histonet] Re: Paraffin Blocks
I've been preaching this sermon for several years!!! We must do something!!! j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, February 05, 2009 5:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Paraffin Blocks How long to retain paraffin blocks (human tissue) is an issue that's going to need re-thinking. Presently most pathology services in the USA retain them for no more than ten years. If the laboratory closes, slides and blocks are disposed of immediately. It seems to me that in the coming age of molecular pathology, there will be a lot more interest in recovering paraffin blocks after longer periods of time. I think that the regulatory agencies will soon need to address this problem. Storing present-day paraffin blocks, with their bulky plastic mounts and low-melting waxes, is going to become even more difficult than it is now, as hospitals and other facilities consider the cost of maintaining and cooling storage space. I don't think it's possible to triage blocks and slides for disposal at the time the case is signed out. It sounds like a good idea, but the few times I've seen it tried it didn't work. Also, we really don't know what we're going to need to recover in the future. Bob Richmond Samurai Pathologist (nearly 70, isn't going to have to worry about this problem) Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Paraffin Blocks
How long to retain paraffin blocks (human tissue) is an issue that's going to need re-thinking. Presently most pathology services in the USA retain them for no more than ten years. If the laboratory closes, slides and blocks are disposed of immediately. It seems to me that in the coming age of molecular pathology, there will be a lot more interest in recovering paraffin blocks after longer periods of time. I think that the regulatory agencies will soon need to address this problem. Storing present-day paraffin blocks, with their bulky plastic mounts and low-melting waxes, is going to become even more difficult than it is now, as hospitals and other facilities consider the cost of maintaining and cooling storage space. I don't think it's possible to triage blocks and slides for disposal at the time the case is signed out. It sounds like a good idea, but the few times I've seen it tried it didn't work. Also, we really don't know what we're going to need to recover in the future. Bob Richmond Samurai Pathologist (nearly 70, isn't going to have to worry about this problem) Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Journal achieves
I had no idea there was anyone out there who really wanted them!!! 29 people have responded the first 3 hours. Last count was 48.I will be making arrangements with Marla Thomas for the JOH. But the books will have to wait for awhile. And for all of you who wanted booksI kept your names and address. Thank you for all the responses and "Best Wishes" Barbara Barbara Stancel, HTL(ASCP)HT USDA, FSIS, EL, Pathology Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stan...@fsis.usda.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Career Opportunity for Histotechnology Manager
HI All, Labforce Services is currently conducting a national search for an HT manager for an internationally renowned NYC (Manhattan) Hospital. The successful candidate will manage a very busy HT Lab staffed by a well compensated and seasoned staff of professionals. Working in the Manhattan the person selected "main lab" the person selected will also be responsible for a number of smaller satellite labs. The institution is extremely well funded (no recession here) and the lab budget for both personnel and equipment is extraordinary. This position offers a geneous sign on bonus in lieu of relocation reimbursement. For the right person there is the possibility in helping with finding appropriate housing. The salary starts around 105k but the institution will pay what is necessary within reason for this spot. Requirements: HT ASCP NYS license (or willing to get one) and previous HT management experience in a large tertiary care hospital or Cancer Center. Please call me toll free at 800-221-0081. If you are not interested personally please feel free to forward this email to a colleague that might be. Regards, Bill Connolly LABFORCE SERVICES bconno...@labforceservices.com WORK: 516-625-8200 TOLL FREE: 800-221-0081 FAX: 516-625-0629 “Be kind, for everyone you meet is fighting a harder battle.” - Plato ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Block Keepin'
I'm (obviously) in a vet diagnostic lab. We had historically kept blocks 10 years; space was tight; the boss sent me on a Research Mission to Find Out How Long Other Vet Labs Kept Their Blocks. I did and made the Executive Decision (well, it was Friday and I figured I could at least live through the weekend), to keep blocks for seven (7) years; boss agreed. And I'm still here. Time seemed to be appropriate to the veterinary world. Slides? - that's chiseled in stone: TEN years. Hope this helps. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laser microdissection of flourescently labelled cells
Does anyone have experience in laser microdissecting fluorescently immuno-labeled cells and then extracting quality RNA? Nestor Barrezueta ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathology Recruiters
I need the names and contact information of recruiters for Anatomic Pathology positions (specifically Manager and Histology Supervisor positions). Please contact me directly. RWC Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BioCare rep
You can call Joe Meyer...he works there and he is extremely helpful, or you can call my rep --Mark Castagnini...he is always helpful or ask for Chrisshe will be glad to assist, If you need any of these numbers let mek now I will be glad to give them to you offline. -Original Message- From: Angela Bitting To: gu.l...@gmx.at; histonet@lists.utsouthwestern.edu Sent: Thu, 5 Feb 2009 12:06 pm Subject: [Histonet] BioCare rep Can anyone tell me how to get in contact with Kathy Bowden from Biocare? I left a message at Biocare but she didn't call me back. Maybe one of you has her email or phone number. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BioCare rep
Can anyone tell me how to get in contact with Kathy Bowden from Biocare? I left a message at Biocare but she didn't call me back. Maybe one of you has her email or phone number. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] prediluted Fitc labelled antibodies?
Gudrun: I used to predilute the FITC conjugated antibodies and placed the aliquots in a -80ºC freezer until they were going to be used. In that way I could use them until the manufacturer's expiration date. René J. --- On Thu, 2/5/09, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] prediluted Fitc labelled antibodies? To: histonet@lists.utsouthwestern.edu Date: Thursday, February 5, 2009, 11:37 AM Hi all, Has anyone experience with the shelflife of prediluted FITC-labelled antibodies? We use them for direct Immunofluorescence and dilute usually with PBS on the same day of use. With the increasing amount of IF-tests we consider prediluting the antibodies. But I don't know, if there is any negative influence. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mounting Media Question
We have been using the Sakura Glas Coverslipper for about 4 years. We love it. It's our 5th tech. We use the Tissue-Tek Glas #6419 (Sakura) mounting media. It is the right consistency (no dilution needed), non yellowing, open the top and stick on the machine. We usually don't file our slides for several days so I cannot attest to its drying speed. We use ONLY Richard Allan Scientific "Slip-Rite" coverglasses, 24 x 50. Volume setting 4 and speed setting 5. Be sure the xylene in the tray does not get too high, never up to the frost of the slide. The vacuum cup will get damp which will affect the vacuum holding the slidenot good! Always, Always keep the lubrication bottle and nozzle reservoir full of xylene. We have PM done yearly. The last 4 sentences are the voice-of-experience!!! If a "technical error" is possible, we've done it!! Enjoy your new coverslipper!! Barbara Barbara Stancel, HTL(ASCP) USDA, FSIS, EL, Pathology Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stan...@fsis.usda.gov Message: 11 Date: Wed, 04 Feb 2009 10:56:17 -0600 From: "LINDA MARGRAF" Subject: [Histonet] Mounting media question To: Message-ID: <49897450.f783.00d...@childrens.com> Content-Type: text/plain; charset="us-ascii" We will soon be receiving our new Sakura Glas Coverslipper. Can anyone who is also using this instrument please tell me what type of mounting media is being used. We are looking for a fast-drying media that can be used for tissue slides, non-gyne and gyne slides. We are also looking at long term storage without fading capability. Our current mounting media - Surgipath's Micromount used on the older model Sakura -Glas coverslipper does not dry quickly. Storage and slide handling issues around this problem. Thank you, Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory Chinook Regional Hospital Message: 12 Date: Wed, 4 Feb 2009 09:09:01 -0800 (PST) From: Rene J Buesa Subject: [Histonet] Mounting media question To: histonet@lists.utsouthwestern.edu, LINDA MARGRAF Message-ID: <549235.49508...@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The Fisher Permount (SP15) is ideal for what you describe. The main advantage is that you can dilute it to your needs without losing any of its properties. René J. --- On Wed, 2/4/09, LINDA MARGRAF wrote: From: LINDA MARGRAF Subject: [Histonet] Mounting media question To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 4, 2009, 11:56 AM We will soon be receiving our new Sakura Glas Coverslipper. Can anyone who is also using this instrument please tell me what type of mounting media is being used. We are looking for a fast-drying media that can be used for tissue slides, non-gyne and gyne slides. We are also looking at long term storage without fading capability. Our current mounting media - Surgipath's Micromount used on the older model Sakura -Glas coverslipper does not dry quickly. Storage and slide handling issues around this problem. Thank you, Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory Chinook Regional Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] prediluted Fitc labelled antibodies?
Hi all, Has anyone experience with the shelflife of prediluted FITC-labelled antibodies? We use them for direct Immunofluorescence and dilute usually with PBS on the same day of use. With the increasing amount of IF-tests we consider prediluting the antibodies. But I don't know, if there is any negative influence. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Borrelia photo
I found this picture on immunoportal.com. But I was told, that Borrelia never sit in the epidermis. Can anyone confirm this? http://www.immunoportal.com/modules.php?set_albumName=album01&id=borreliab&o p=modload&name=Gallery&file=index&include=view_photo.php bye Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Jan Shivers Gesendet: Mittwoch, 04. Februar 2009 21:30 An: histonet Betreff: [Histonet] Borrelia photo Can anyone direct me to a good photo of an IHC-stained Borrelia burgdorferi (+) tissue? Thanks in advance, Jan Shivers UMN VDL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
