[Histonet] Inflammation cell marker on Tissue & Quantitive Immunohistofluorscence
Dear All: Just wonder any one have the experience to work on inflammation on different tissues, especially Liver, SKIN, and Brain? We want to look at wound-resulted inflammation level in these tissues, using immunohistochemical techniques rather RT-PCR / WB on cytokine levels. Can anyone recommend different cell markers for these tisses, separately? Another question is how about the Quantitive Immunohistofluorscence. I have some sections stained with Ed-1, marker of macrophage. It is very hard to count the number of positive cells ...can we use immunofluorescence instensity (same exposure time) or the area of positive region as the quantitive index? At least semi-qunatitive. 2009-02-25 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Giemsa Stain
I'd say underfixation with 10% NBF. Try extending the time in formalin, before going into decal. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caleri, Kathleen Sent: Tuesday, February 24, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Giemsa Stain Our lab was using B-5 to fix our bone marrow specimens, and the resulting Giemsa stains were very nice...In order to use the pneumatic tube system at the facility we had to switch to 10% NBF ( we are in the process of choosing a zinc formalin fixative) and now the Giemsas are a funny turquoise, and very palehas anyone ever encountered this? any ideas?? Regards, Kate Kathleen Caleri, BS, MLT, HT (ASCP) Histology Lab Supervisor Pathology Roswell Park Cancer Institute (716) 845-1329 "the future of mankind lies waiting for those who come to understand their lives and take up their responsibilities to help others" This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: BVD Antibody
IDEXX - they bought the Ab rights to the clone previously produced and sold by Cornell. But it's very hard to get them to sell it to you. They use it to create their own ELISA test kits, I believe. Jan Shivers UMN VDL - Original Message - From: "Breeden, Sara" To: Sent: Tuesday, February 24, 2009 2:04 PM Subject: [Histonet] BVD Antibody I need a source for BVD antibody and would appreciate suggestion. Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] BVD Antibody
Don't fight it... It will soon be Friday.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Tuesday, February 24, 2009 3:20 PM To: histonet@lists.utsouthwestern.edu; Sara Breeden Subject: Re: [Histonet] BVD Antibody I'm fighting a compulsion to make a bad underwear joke. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Breeden, Sara" 2/24/2009 3:04 PM >>> I need a source for BVD antibody and would appreciate suggestion. Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Laser Capture Microdissection System
we have the zeiss PALM lcm machine... love it. Kimberly Tuttle wrote: Im looking at Leica, Zeiss, or Arcturus. If you have one of these, let me know how you like it. Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cutting teeth
Andi, you may want to contact Bob Skinner directly with your questions. skinnerrobe...@uams.edu I worked with Bob for about twelve years and he was and as far as I know is still using methyl salicylate in place of xylene as a clearing agent. Methyl salicylate avoids the brittleness and hardening that can occur with xylene. I don't know if he's compared his clearing technique with Clear Rite. Bob's work is in orthopaedics, I don't know if he's done much with teeth recently but I know he did some eons ago. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, February 24, 2009 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting teeth I'm hoping that this isn't as painful as the actual process we all went through as children. I'm told that one of the investigators here will be sending a couple hundred teeth to my lab soon. I have never processed or sectioned teeth before so I'm hoping that I can find a few good suggestions on histonet. Here is what they are doing - based on two papers from JOE in 05 and 07: Fixation is in 10% NBF - how long do you fix teeth? Is temperature something to consider here? Decalcification is done in a solution of equal parts of 50% formic acid and 20% sodium citrate. The papers say 10 days is enough to decalcify teeth. Is it enough time? Following decal they infiltrated the teeth using "Skinner's method". Anybody know what this involves? One paper says Methyl salicylate was used as a clearing agent. Why is this better than traditional processing on my VIP using Clear Rite 3 as a clearing agent? In the papers the teeth were embedded in Paraplast and sectioned at 5 microns and then thinner, they actually want thinner sections - like 2 microns. We are looking for pulp tissue. Then they want a Brown and Brenn stain and maybe a trichrome. Thanks, Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BVD Antibody
Did you check VMRD? http://www.vmrd.com/products/antibodies/detailInfIG.aspx?CATNO=D89 Bovine Viral Diarrhea Virus MAb (gp55) Catalog No.: D89 Description: Binds to 55 kDa of BVDV. Made using NADL strain. Binds to most BVDV strains. Does not bind Oregon C24V strain. IgG2a isotype. Suitable for immunofluorescence, immunohistochemistry and virus neutralization. This monoclonal antibody is produced as mouse ascites fluid, clarified by centrifugation, and filtered through a 0.2 micrometer filter. The concentration is 1.0 mg/ml in phosphate-buffered saline, preserved with 10 ppm ProClin 300. Format: Mouse ascites Size: 0.1, 0.5 and 1 mg Expiration: One year from date of QC release. Storage: Store at 2-7°C. Do not freeze! Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. Déclaration de confidentialité Le présent message (y compris les annexes) peut contenir des renseignements confidentiels à l'intention d'une personne ou d'un organisme particulier. Si vous avez reçu la présente communication par erreur, veuillez en informer l'expéditeur immédiatement. Si vous n'êtes pas le destinataire prévu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer immédiatement de votre système informatique. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BVD Antibody
I'm fighting a compulsion to make a bad underwear joke. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Breeden, Sara" 2/24/2009 3:04 PM >>> I need a source for BVD antibody and would appreciate suggestion. Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] BVD Antibody
For IHC? Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, February 24, 2009 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BVD Antibody I need a source for BVD antibody and would appreciate suggestion. Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BVD Antibody
I need a source for BVD antibody and would appreciate suggestion. Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cutting teeth
I'm hoping that this isn't as painful as the actual process we all went through as children. I'm told that one of the investigators here will be sending a couple hundred teeth to my lab soon. I have never processed or sectioned teeth before so I'm hoping that I can find a few good suggestions on histonet. Here is what they are doing - based on two papers from JOE in 05 and 07: Fixation is in 10% NBF - how long do you fix teeth? Is temperature something to consider here? Decalcification is done in a solution of equal parts of 50% formic acid and 20% sodium citrate. The papers say 10 days is enough to decalcify teeth. Is it enough time? Following decal they infiltrated the teeth using "Skinner's method". Anybody know what this involves? One paper says Methyl salicylate was used as a clearing agent. Why is this better than traditional processing on my VIP using Clear Rite 3 as a clearing agent? In the papers the teeth were embedded in Paraplast and sectioned at 5 microns and then thinner, they actually want thinner sections - like 2 microns. We are looking for pulp tissue. Then they want a Brown and Brenn stain and maybe a trichrome. Thanks, Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cheryl
I totally agree with you Cheryl!!! Amy, HT Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laser Capture Microdissection System
Im looking at Leica, Zeiss, or Arcturus. If you have one of these, let me know how you like it. Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full-Time histology position in Fayetteville, NC
Hi Histoland, Cape Fear Valley Medical Center in Fayetteville, NC is currently looking for a full-time histotechnician or histotechnologist. For more information and/or to apply, please see the posting on the hospital's career website: www.capefearcareers.com Have a great day, Kelly Colpitts, HT(ASCP) _ It’s the same Hotmail®. If by “same” you mean up to 70% faster. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_AE_Same_022009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histo wrap
I order direct from Obex Industries, who makes Histowrap. __ Does anyone have a vendor for Histo Wrap - we have found this to be the best in terms of filtering? Thank you in advance Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Giemsa Stain
Our lab was using B-5 to fix our bone marrow specimens, and the resulting Giemsa stains were very nice...In order to use the pneumatic tube system at the facility we had to switch to 10% NBF ( we are in the process of choosing a zinc formalin fixative) and now the Giemsas are a funny turquoise, and very palehas anyone ever encountered this? any ideas?? Regards, Kate Kathleen Caleri, BS, MLT, HT (ASCP) Histology Lab Supervisor Pathology Roswell Park Cancer Institute (716) 845-1329 "the future of mankind lies waiting for those who come to understand their lives and take up their responsibilities to help others" This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Electron Microscopy
Hello Histoland! I am seeking an individual with Electron Microscopy experience... please contact me if you have it or know of someone that does. Thanks! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asac...@titanmed.com see us on the web at www.titanmed.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Practical Exam, RE: testing cutting ability
Where is the fence??? It looks like a P---ing match between dogs. We ALL worked hard on our certification no matter how we got it. And due to the shortage I assume we ALL work hard now. Fast or slow matters not. Histology, good histology needs many talents such as; scientific knowledge, troubleshooting, manual dexterity and artistry...We all create. Again, my 2 cents and please understand my intention is not to incite anger. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue disposal
We decant the formalin off, then put the tissue into pathology waste boxes, which are then incinerated. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbu...@yahoo.com] Sent: Tuesday, February 24, 2009 9:27 AM To: Histonet@lists.utsouthwestern.edu; Weems, Joyce Subject: Re: [Histonet] Tissue disposal We used to incinerate it on site. René J. --- On Mon, 2/23/09, Weems, Joyce wrote: From: Weems, Joyce Subject: [Histonet] Tissue disposal To: Histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 3:35 PM How are people disposing of their tissue? Especially in the Atlanta area? Inquiring minds want to know. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: testing cutting ability--This isstarting to ReallyAnnoy me.
It would probably take too long to recount anyway... Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of JoeNocito Sent: Mon 2/23/2009 7:33 PM To: 'Rittman, Barry R'; 'Peter Carroll' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: testing cutting ability--This isstarting to ReallyAnnoy me. I'll never tell -Original Message- From: Rittman, Barry R [mailto:barry.r.ritt...@uth.tmc.edu] Sent: Monday, February 23, 2009 6:32 PM To: JoeNocito; 'Peter Carroll' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: testing cutting ability--This is starting to ReallyAnnoy me. Joe how many previous lives did you have, and what were you in each of them? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC kits
Hello, I have been using the ABC kits from Vector Labs and have had great success typically. Does anyone have an recommendations on other IHC kits such as DAKO or Invitrogen? Does anyone have any input as to which kits are best for IHC? Is it worth trying to change over to another kit to save some time/steps in protocol or hope for better sensitivity? Any recommendations will be greatly appreciated. Thanks. Kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue disposal
We used to incinerate it on site. René J. --- On Mon, 2/23/09, Weems, Joyce wrote: From: Weems, Joyce Subject: [Histonet] Tissue disposal To: Histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 3:35 PM How are people disposing of their tissue? Especially in the Atlanta area? Inquiring minds want to know. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue disposal
SteriCycle takes ours...we have to use yellow barrels labeled "pathology waste" and try to seperate "identifiable" tissue from "unidentifiable" tissues, which for us would be limbs, products of conception (if there is a fetus that could be recognized), and some mastectomies...Once Stericycle takes it, they incenerate it so that nothing can be identified. I'm not sure what they do with the ashes after that. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks --- On Mon, 2/23/09, Weems, Joyce wrote: From: Weems, Joyce Subject: [Histonet] Tissue disposal To: Histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 1:35 PM How are people disposing of their tissue? Especially in the Atlanta area? Inquiring minds want to know. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue disposal
SteriCycle takes ours...we have to use yellow barrels labeled "pathology waste" and try to seperate "identifiable" tissue from "unidentifiable" tissues, which for us would be limbs, products of conception (if there is a fetus that could be recognized), and some mastectomies...Once Stericycle takes it, they incenerate it so that nothing can be identified. I'm not sure what they do with the ashes after that. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks --- On Mon, 2/23/09, Weems, Joyce wrote: From: Weems, Joyce Subject: [Histonet] Tissue disposal To: Histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 1:35 PM How are people disposing of their tissue? Especially in the Atlanta area? Inquiring minds want to know. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue disposal
SteriCycle takes ours...we have to use yellow barrels labeled "pathology waste" and try to seperate "identifiable" tissue from "unidentifiable" tissues, which for us would be limbs, products of conception (if there is a fetus that could be recognized), and some mastectomies...Once Stericycle takes it, they incenerate it so that nothing can be identified. I'm not sure what they do with the ashes after that. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks --- On Mon, 2/23/09, Weems, Joyce wrote: From: Weems, Joyce Subject: [Histonet] Tissue disposal To: Histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 1:35 PM How are people disposing of their tissue? Especially in the Atlanta area? Inquiring minds want to know. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo wrap
Does anyone have a vendor for Histo Wrap - we have found this to be the best in terms of filtering? Thank you in advance Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: testing cutting ability--This is starting toReallyAnnoy me.
Peter, Don't start with the research and blocks per hour- I had a case where I had step and serial at each step with 8 sections on the slide and had to exhaust the block, so 1 block every 20-30 minutes is not hard to come by. We also deal with clinical trials and that means sterile sectioning so cleaning time etc means maybe 30 blocks in a day (we cut a fair amount of slides for correlative studies) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Monday, February 23, 2009 3:26 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: testing cutting ability--This is starting toReallyAnnoy me. > Jeez, are clinical histopath labs just sweat-shops? Pretty much. Having spent years doing path at a huge local hospital which featured a slave-driving lab director who was less qualified to run the lab than half the people working in it, I must say that I agree with this observation. At the same time, as much as I enjoy my more laid-back research path position now, I'd get fired in a heartbeat if I produced only one block every 30 minutes. Actually, I just got finished doing closer to 40 in the last hour, and today was a fairly slow day (yet not at all on par with the old clinical stats, were 8-12 techs embedded/sectioned closer to 800-1000 blocks per shift). Charles.Embrey wrote: > Jerry, you are right that clinical work is different from research. You are wrong to consider 20 blocks per hour to be a "sweat-shop" pace. I've know scores of histotechs that cut well over 20 blocks per hour and produce high quality slides. Sorry you feel offended by this discussion but there is a real world outside of your university walls. > Chuck > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R > Sent: Monday, February 23, 2009 1:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: testing cutting ability--This is starting to ReallyAnnoy me. > > > All this talk of having people actually section as part of the interview process is offensive to me. > > I have hired and trained lots of histotechnologists. Mostly I train them myself, from scratch, but sometimes I hire them pre-trained. Then of course, I re-train them anyway so they can do the work to the specs of my lab. Any deficiencies they have will be corrected through training. By me. > > At my University there is a formal 30 day (maybe it's longer, I can't recall) probationary period for new hires. That's good enough. Why in the world would I need to have them section as part of an interview process? > > I am also offended by the "blocks per hour" language. Partly it's because in my lab we do exhaustive serial sectioning, so I consider a tech who gives me one block every half-hour to be doing a good job. > > Maybe it's different in clinical work, but...20 blocks per hour? And you expect quality work? > > Jeez, are clinical histopath labs just sweat-shops? > > It's no wonder histopathologists feel overworked and underappreciated. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > > > > > >> Date: Mon, 23 Feb 2009 10:14:06 -0500 >> From: trathbo...@somerset-healthcare.com >> To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; tbr...@holyredeemer.com >> Subject: RE: [Histonet] RE: testing cutting ability during an interview >> CC: >> >> We also have the staff talk to the applicant during this process. It gives you a good idea if the person can talk and cut at the same time. The last thing anyone wants is a histotech that has to stop sectioning during a conversation! >> >> -Original Message- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J >> Buesa >> Sent: Monday, February 23, 2009 10:05 AM >> To: histonet@lists.utsouthwestern.edu; Terri Braud >> Subject: Re: [Histonet] RE: testing cutting ability during an interview >> >> >> I for one always required any applicant to prepare 20 slides stained with H&E. I can assure that I selected "difficult" blocks to cut and the applicant was required to sign a disclaimer that included that s/he he knew how to section and avoid injuries. >> The whole process was timed (to get a first idea about productivity) and I evaluated and graded the slides at the end. >> The results were used as one of the elements to decide about offering the position (the fundamental) but I waited until all the applicants had completed the tests so sometimes the applicant had to be contacted a few days later to let him/her know about the results. >> René J. >> >> --- On Mon, 2/23/09, Terri Braud wrote: >> >> From: Terri
[Histonet] Streck's Fixative
Hi Everyone, I am reading an article in the latest JHC about detection of phospho-proteins with the use of Streck's fixative. I would like to try some of this fixative, but I can't seem to find it on the Streck labs website. It is listed on the Fisher website, but the item has apparently been discontinued. Can someone tell me how to order this? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: testing cutting ability--This is starting to ReallyAnnoy me.
I'm not sure why asking someone to cut on an interview would be offensive? I have been asked to demonstrate my technical skills on interviews and had no problem with it whatsoever. The way I look at it, the histology role has at least two aspects- the theoretical and scientific background knowledge and the technical proficiency. I think a manager can get some inference about your knowledge from your resume and education, but that does not tell them much about your technical abilities. I think that it is perfectly fair to get some measure and insight into the other half by asking for a demonstration. It is really a practical assessment in my opinion, as to whether your combination of knowledge and technical skill level is a good fit for that particular lab situation. Clearly, in a large clinical lab being able to produce the volume and maintain the quality is key. Perhaps in a research area, education or theoretical knowledge might be more important. I think that if you are confident of your abilities in all areas, then this would not be any sort of offense? On another note, neither your resume or your slides will tell them much about your personality "fit", attendance or general attitude, but that is what references are for, and also the probationary period. Joelle Weaver HTL (ASCP) > Date: Mon, 23 Feb 2009 16:26:12 -0500 > From: carro...@umdnj.edu > CC: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: testing cutting ability--This is starting to > ReallyAnnoy me. > > > Jeez, are clinical histopath labs just sweat-shops? > > Pretty much. Having spent years doing path at a huge local hospital > which featured a slave-driving lab director who was less qualified to > run the lab than half the people working in it, I must say that I agree > with this observation. > > At the same time, as much as I enjoy my more laid-back research path > position now, I'd get fired in a heartbeat if I produced only one block > every 30 minutes. Actually, I just got finished doing closer to 40 in > the last hour, and today was a fairly slow day (yet not at all on par > with the old clinical stats, were 8-12 techs embedded/sectioned closer > to 800-1000 blocks per shift). > > > > Charles.Embrey wrote: > > Jerry, you are right that clinical work is different from research. You are > > wrong to consider 20 blocks per hour to be a "sweat-shop" pace. I've know > > scores of histotechs that cut well over 20 blocks per hour and produce high > > quality slides. Sorry you feel offended by this discussion but there is a > > real world outside of your university walls. > > Chuck > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu > > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R > > Sent: Monday, February 23, 2009 1:58 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: testing cutting ability--This is starting to > > ReallyAnnoy me. > > > > > > All this talk of having people actually section as part of the interview > > process is offensive to me. > > > > I have hired and trained lots of histotechnologists. Mostly I train them > > myself, from scratch, but sometimes I hire them pre-trained. Then of > > course, I re-train them anyway so they can do the work to the specs of my > > lab. Any deficiencies they have will be corrected through training. By me. > > > > At my University there is a formal 30 day (maybe it's longer, I can't > > recall) probationary period for new hires. That's good enough. Why in the > > world would I need to have them section as part of an interview process? > > > > I am also offended by the "blocks per hour" language. Partly it's because > > in my lab we do exhaustive serial sectioning, so I consider a tech who > > gives me one block every half-hour to be doing a good job. > > > > Maybe it's different in clinical work, but...20 blocks per hour? And you > > expect quality work? > > > > Jeez, are clinical histopath labs just sweat-shops? > > > > It's no wonder histopathologists feel overworked and underappreciated. > > > > Jerry Ricks > > Research Scientist > > University of Washington > > Department of Pathology > > > > > > > > > > > > > >> Date: Mon, 23 Feb 2009 10:14:06 -0500 > >> From: trathbo...@somerset-healthcare.com > >> To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; > >> tbr...@holyredeemer.com > >> Subject: RE: [Histonet] RE: testing cutting ability during an interview > >> CC: > >> > >> We also have the staff talk to the applicant during this process. It gives > >> you a good idea if the person can talk and cut at the same time. The last > >> thing anyone wants is a histotech that has to stop sectioning during a > >> conversation! > >> > >> -Original Message- > >> From: histonet-boun...@lists.utsouthwestern.edu > >> [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J > >> Buesa > >> Sent: Monday, February 23,
RE: [Histonet] Practical Exam
No, I believe "Rat's Patout" was originally a Cajun song, by Lionel and the Mossy Banks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JoeNocito Sent: 24 February 2009 00:07 To: 'Ingles Claire '; 'Histonet' Subject: RE: [Histonet] Practical Exam A rat's patoot? Is that like a rodent's behind? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, February 23, 2009 4:59 PM To: Histonet Subject: RE: [Histonet] Practical Exam H... I remember a fellow tech. Lazy SOB. He had passed the test for the HTL, but couldn't pass the practical, because he really didn't know what he was doing (or cared). He once asked me if I would stain his registry slides along with our regular run of special stains. Needless to say I said NO! He couldn't understand why. (course I don't think he ever understood why his charm never worked on me either) I don't know if he ever passed the exam. I think he was at least on his second attempt at the practical. I also knew a path resident that was even lazier than the 'histotech' and didn't give a rat's patoot either. THAT really gave me the shutters/steamed me up. Then again, a lot of the Path residents didn't like me because I would take tissue back to them to recut because it was too thick to process adequately. Extra work you know. I'm so glad I only have to deal with one fellow a year now. They are much better to begin with. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Fri 2/20/2009 3:11 PM To: 'Podawiltz, Thomas'; rjbu...@yahoo.com; 'Histonet'; 'O'Donnell, Bill' Subject: RE: [Histonet] Practical Exam Hi, This has nothing to do with Bill or you Tom however, when the practical was still being given people did come on histonet and ask for tissue in blocks and help finishing the exam. It was not a good thing and showed that some people were not able to acquire the tissues needed or were not looking for them. Since I saw this several times and many of us were upset about it, I can say at least a few people did not do their own if they could avoid it. I don't remember names or locations so please don't ask for them. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT certifacation
The OJT route does say a minimum of 60 semester hours (90 quarter hours) with 12 semester hours (18 quarter hours) of biology and chemistry. This allows for someone with, for example, 3 years of college (90 semester hours) to take the HT exam. They don't have an associate degree, they don't don't a baccalaurate degree. They are somewhere in between. This person can therefore take the HT exam, as they have more than the minimum 60 semester hours that is required (which is about what is needed for an associate degree). Just for clarification, it's 12 semester hours of biology and chemistry combined. Not 12 semester hours each. So if each course is 4 semester hours, then two biology classes and one chemistry class would qualify. Or one biology and two chemistry classes. Peggy A. Wenk, HTK(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy Gorham Sent: Sunday, February 22, 2009 7:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT certifacation I have two young people in our lab that would like to get certified as a HT. One is looking into the Harford Community College on line and the other is trying for the "At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry ". I have three questions. Does anyone know anything about the Harford School? Has anyone had a student from there to do their practicum in your lab? and I just want to be sure that the second student does not have to have an associate degree any longer; Just the 60 semester hours and 12 of biology/chemistry is that correct? Thank you Kathy Gorham H.T. Grande Ronde Hospital La Grande Oregon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
