RE: [Histonet] Re: Log Book

[Norton, Sally]

Kathy

We use the same method as Michelle described.  We mark next to the patient name 
if there is more that one specimen.   We do not take the specimen if there is 
any discrepancy.  The OR has to fix it first and sign a form taking 
responsibility for the specimen.

Sally
Seattle Children's Hospital


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shelly Coker
Sent: Tuesday, March 03, 2009 4:22 PM
To: histonet@lists.utsouthwestern.edu; gorh...@verizon.net
Subject: [Histonet] Re: Log Book

Kathy,
 
I worked in a hospital at one time where we were expected to pick up the 
specimens from the OR.  We actually created a log book that stayed in the OR.  
The nurses simply placed a patient label in a spiral notebook and noted the 
specimens beside it.  When we went to pick them up, we checked each specimen 
off and put our initials, date and time in the book. We would not pick up any 
specimens with a discrepancy until the nursing staff corrected it.  In the 
instance you are describing, the specimens for said patient would not have been 
picked up until the nursing staff corrected the log book or found the 
specimen.  We did also date/time stamp the requisitions when we got back to the 
lab as well.
 
Good luck and I hope you find your specimen :(   
 
Michelle

Good Monday Morning,  We had a serious incident Friday with O.R.  My aide went
down to get the specimens from O.R. about 9am. (which were left overs from the
night before).  She did not stamp in the specimens before she left.  When I had
time to stamp them in and record them in the log book I discovered that the
colon was not there.  Two other specimens from that patient where in the bag but
no colon.  So I went down to O.R. to see where it was.  Of course no one knows
what happened to the colon.  The doctors are furious by all means.  Now the O.R.
thinks the path lab screwed up.  So my questions is how do others log in the
specimens as they come into the lab.  We have 2 couriers that brings specimens
when we are not in the lab from other hospitals.  How do you make sure that whom
ever brings the specimens actually brings the ones they say they do?  Do you
have a log book that every specimen that is brought into the lab is written down
by the person who brings it in?  Right now we have a log book but it is written
in as we are accessing  the specimens.  So the specimens may have been there
overnight. We are a very small lab and we do almost everything by hand including
writing in the log book.  Someday we want to be able to scan by bar codes but
right now we can not do that. Thanks for any help you can give me. 
Kathy Gorham, H.T


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Children's Hospital and Regional Medical Center is now Seattle Children's.
CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information protected by law.  Any unauthorized review, use, 
disclosure or distribution is prohibited.  If you are not the intended 
recipient, please contact the sender by reply e-mail and destroy all copies of 
the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Log Book

[Shelly Coker]

Kathy,
 
I worked in a hospital at one time where we were expected to pick up the 
specimens from the OR.  We actually created a log book that stayed in the OR.  
The nurses simply placed a patient label in a spiral notebook and noted the 
specimens beside it.  When we went to pick them up, we checked each specimen 
off and put our initials, date and time in the book. We would not pick up any 
specimens with a discrepancy until the nursing staff corrected it.  In the 
instance you are describing, the specimens for said patient would not have been 
picked up until the nursing staff corrected the log book or found the 
specimen.  We did also date/time stamp the requisitions when we got back to the 
lab as well.
 
Good luck and I hope you find your specimen :(   
 
Michelle

Good Monday Morning,  We had a serious incident Friday with O.R.  My aide went
down to get the specimens from O.R. about 9am. (which were left overs from the
night before).  She did not stamp in the specimens before she left.  When I had
time to stamp them in and record them in the log book I discovered that the
colon was not there.  Two other specimens from that patient where in the bag but
no colon.  So I went down to O.R. to see where it was.  Of course no one knows
what happened to the colon.  The doctors are furious by all means.  Now the O.R.
thinks the path lab screwed up.  So my questions is how do others log in the
specimens as they come into the lab.  We have 2 couriers that brings specimens
when we are not in the lab from other hospitals.  How do you make sure that whom
ever brings the specimens actually brings the ones they say they do?  Do you
have a log book that every specimen that is brought into the lab is written down
by the person who brings it in?  Right now we have a log book but it is written
in as we are accessing  the specimens.  So the specimens may have been there
overnight. We are a very small lab and we do almost everything by hand including
writing in the log book.  Someday we want to be able to scan by bar codes but
right now we can not do that. Thanks for any help you can give me. 
Kathy Gorham, H.T



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Help with McDowell and Trump's Fixative 4F:1G for EM samples

[Susan Raibley]

Hello!  Can anyone give me some info on McDowell and Trump's fixative?  We have 
a protocol that requests the formulation of 4F:1G of McDowell and Trump's 
fixative that we are going to be collecting electron microscopy samples in.  
Does anyone know if this is a specific formulation that must be made up, or is 
it a common formulation that can be bought ready made?  Thanks! 
 
Susan Bincsik
BASi, Histology Technician



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] looking for aVb3 antibody for IHC on FFPE tissue

[Connolly, Brett M]

any favorites out there for integrin alphaV beta 3?

Thanks

Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
e-mail. brett_conno...@merck.com

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates (which may be known
outside the United States as Merck Frosst, Merck Sharp & Dohme or
MSD and in Japan, as Banyu - direct contact information for affiliates is
available at http://www.merck.com/contact/contacts.html) that may be
confidential, proprietary copyrighted and/or legally privileged. It is
intended solely for the use of the individual or entity named on this
message. If you are not the intended recipient, and have received this
message in error, please notify us immediately by reply e-mail and
then delete it from your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Sulfated Alcian Blue

[Tony Henwood]

Peggy,

Have you tried the alcian blue tetrakis (methylpyridinium) chloride
(Sigma, Cat. No.A4045, 90% dye content)?

I would be interested to know whether this stains the amyloid better.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Wenk
Sent: Wednesday, 4 March 2009 7:35 AM
To: Nathanial nauss; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Sulfated Alcian Blue


Sulfonate Alcian Blue (SAB) is not always as specific as you would like.

Also, if you are using the newer alcian blue dye, it seems to have a 
different formulation than the old stuff that I have. The new alcian
blue 
will not dissolve in alcohol, so the newer dye doesn't demonstrate the 
amyloid, while my old dye does.

Old deposits of amyloid do not have beta pleats at regular intervals. 
Therefore, the Congo red dye (CR) will still bind, but will not line up
one 
right after the other | | | | |, but will be more random \  _  |  /.
When 
the CR dye are parallel to each other, they will show the apple green 
birefringence with the polarizing microscope. When the CR dye is
randomly 
arranged, there will be no apple green birefringence.

The other time this happens is with very overfixed amyloid, such as
months 
in NBF. Too many cross-links with NBF, so the CR dye can't bind right,
so 
they are not in parallel. We also had this happen once when the autopsy 
tissues were fixed in B5 (mercury fixative, long time ago). The resident

knew amyloid was an immunological problem, so put through tissue fixed
in 
B5. Congo red did not birefringe. So we went back to the NBF stock
bucket, 
submitted new tissue, and they all were wonderfully green birefringent.

The other problem with the SAB is that, if this is old amyloid, and the
beta 
pleats are messed up, SAB depends somewhat on the beta pleats.
Therefore, 
the green will be much paler in older amyloid than with newer amyoid.
(The 
green is due to to the blue of alcian blue staining the amyloid, and the

yellow of picric acid in the van Gieson also staining the amyloid, so
blue 
and yellow make green.)

We just had a case of non-birefringing green Congo red at our hospital a

couple of months ago, where it definitely looked by amyloid on the H&E,
but 
there was no birefringence on the patient's CR (control was great - all
3 
times that we repeated the procedure). Ot wasn't a fixation problem, or
a 
staining problem, but probably an "old amyloid" problem. Our resident
Dr. 
Tom Fennel just gave a talk on it at our state histology's winter
seminar 
Jan. 31, 2009. Here's what we did (all three):

1. View the Congo Red stained slides with a fluorescence microscope,
such as 
in microbiology auramine-rhodamine stain for AFB. When hit with green
light, 
the CR stained amyloid will fluoresce orange.
2. Use Crystal Violet or Methyl violet staining for amyloid. These
depend 
upon the carboxyl  ions of the amyloid for binding, not the beta pleats.
The 
amyloid should be violet, with the background blue/purple. However, it 
doesn't always demonstrate AA amyloid (some are low in surface carboxyl 
ions). So not always as sensitive as CR.
3. Use Thioflavin T or Thioflavin S for amyloid. Staining is (maybe)
with 
the P component of amyloid, not the beta pleats. Use a fluorescence 
microsope, using blue light (FITC filters), and the amyloid will
fluoresce 
yellow. However, other things also fluoresce yellow, such as fibrinoid 
material, JG granules, sometimes elastin, etc. So not as specific as CR.

By knowing your histology and knowing where in the tissue you are
thinking 
that there are amyloid deposits, the above three alternatives are a nice

addition, for when Congo red is not demonstrating the apple green 
birefringence.

Since in our recent case, 3 out of 4 stains demonstrated amyloid (CR 
fluorescent, Crystal violet and TFT were all positive, while CR 
birefringence was negative), it was diagnosed as amyloid, with, I think,
a 
note that older deposits of amyloid sometimes demonstrate no
birefringence 
with CR.

If this person needs help with find staining procedures for CV/MV or 
TFT/TFS, could someone email them? I'm on vacation, and don't have
access to 
my home or work computer.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073


- Original Message - 
From: "Nathanial nauss" 
To: 
Sent: Friday, February 27, 2009 10:42 AM
Subject: [Histonet] Sulfated Alcian Blue


I need some help, has any one used the sulfated alcian blue to stain for

amyloid. We have a case that looks like it should be positive but it is
not 
staining with the Congo Red. Any help would be great.
Nathaniel

RE: [Histonet] Storage of muscles

[Tony Henwood]

Minus 70 degrees
A normal minus 20 freezer tends to dessicate the tissue

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Wednesday, 4 March 2009 7:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Storage of muscles


Hello,
We are having problems with artifact after our muscles have been stored for 
long periods of time. Can anyone tell me how they store their muscle specimens 
for long-term?
 
Thank you,
Adrienne Anderson
Duke University Health System
rennie1...@yahoo.com
 


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*
This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens 
Hospital at Westmead accepts no liability for any consequential damage 
resulting from email containing computer viruses.
**


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] HIER

[Della Speranza, Vinnie]

You will definitely have to optimize for each antigen.

The time your slides are in your retrieval buffer is inversely related to the 
temperature.

The higher the temperature of your buffer, the shorter the time needed for 
retrieval. 
 
There is nothing wrong with retrieving at a lower temperature (lower than 
boiling) however you will have to significantly increase the time in order to 
attain a satisfactory result. If time is not an issue for you, you may be able 
to retrieve at 90-95 degrees (below boiling to avoid boiling off your buffer) 
but you may have to double or even triple the time your slides are left in the 
retrieval buffer at that lower temperature.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Casper Hempel
Sent: Tuesday, March 03, 2009 4:13 PM
To: rjbu...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HIER

Thanks for all your useful comments. It sounds like to choose temperature
over pH depends on the epitope in question more than anything else. We'll
probably have to optimize it for each antigen.
Best regards
Casper

2009/3/2 Rene J Buesa 

> Both temperature and pH go hand in hand and it is very difficult, if not
> impossible, to select one over the other without experimental data.
> The thing is to just boil the container with the pH buffer and once it has
> boiled (usually in 20 minutes) take it out and leave it on the counter for
> another 20 minutes.
> The pH (from 6 to 8) will depend on the epitope that is going to be
> retrieve because some need pH6 and other pH8 and even higher (or lower).
> Seldom pH7 (neutral) is used.
> René J.
>
> --- On *Mon, 3/2/09, Casper Hempel * wrote:
>
> From: Casper Hempel 
> Subject: [Histonet] HIER
> To: histonet@lists.utsouthwestern.edu
> Date: Monday, March 2, 2009, 1:52 PM
>
> Hi histonetters
> We have been talking a lot about how to retrieve your epitopes using a
> microwave in the best possible way.
> We haven't come to an agreement and people in my lab both argue that
> temperature and pH are important issues. Without a doubt both factors are
> important for a proper retrieval, but if you have to focus on one of the
> factors, which would you consider the most important? Temperature or pH?
> The issue is mainly longer incubations of the slides in boiling buffer. The
> buffer is evaporating and the solution/buffer gets less and less pH neutral
> and you need to top up with dH2O. However, if you boil with reduced
> intensity, less evaporation will occur and the pH will remain more stable.
> Do you have any suggestions or comments to this issue?
> Looking forward to your replies.
> Cheers
> Casper
> ___
> Histonet mailing 
> listhisto...@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] HIER

[Casper Hempel]

Thanks for all your useful comments. It sounds like to choose temperature
over pH depends on the epitope in question more than anything else. We'll
probably have to optimize it for each antigen.
Best regards
Casper

2009/3/2 Rene J Buesa 

> Both temperature and pH go hand in hand and it is very difficult, if not
> impossible, to select one over the other without experimental data.
> The thing is to just boil the container with the pH buffer and once it has
> boiled (usually in 20 minutes) take it out and leave it on the counter for
> another 20 minutes.
> The pH (from 6 to 8) will depend on the epitope that is going to be
> retrieve because some need pH6 and other pH8 and even higher (or lower).
> Seldom pH7 (neutral) is used.
> René J.
>
> --- On *Mon, 3/2/09, Casper Hempel * wrote:
>
> From: Casper Hempel 
> Subject: [Histonet] HIER
> To: histonet@lists.utsouthwestern.edu
> Date: Monday, March 2, 2009, 1:52 PM
>
> Hi histonetters
> We have been talking a lot about how to retrieve your epitopes using a
> microwave in the best possible way.
> We haven't come to an agreement and people in my lab both argue that
> temperature and pH are important issues. Without a doubt both factors are
> important for a proper retrieval, but if you have to focus on one of the
> factors, which would you consider the most important? Temperature or pH?
> The issue is mainly longer incubations of the slides in boiling buffer. The
> buffer is evaporating and the solution/buffer gets less and less pH neutral
> and you need to top up with dH2O. However, if you boil with reduced
> intensity, less evaporation will occur and the pH will remain more stable.
> Do you have any suggestions or comments to this issue?
> Looking forward to your replies.
> Cheers
> Casper
> ___
> Histonet mailing 
> listhisto...@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: bone histology and storage

[Swain, Frances L]

We wrap the bone in saline soaked gauze and freeze at -20 degrees C for bone 
strength testing.  If we receive bone for histomorphometric analysis we store 
our bones in 100% Alcohol.  If the Bones are for paraffin sectioning we fix for 
at least 3 days wash in running tap water and store in 70% EtOh.  We prefer to 
go ahead and process the specimens into paraffin blocks but if the specimens 
are going to be used for something else then we take them to 70% EtOh.  For 
Bone Mechanical testing which I believe you are looking for.  By removing the 
bone, wrapping in saline soaked gauze and freezing does not hurt the tensile 
strength.  When we get ready to test for 3 point bending,etc we thaw the bone 
test and then destroy. 

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfranc...@uams.edu email
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breisch, Eric
Sent: Tuesday, March 03, 2009 2:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone histology and storage

Does anyone have a methodology for long term storage of bone? Formalin
vs alcohol? Orthopedic group here is wondering whether bone for research
purposes should be preferentially stored in alcohol. They aren't
interested in immunohistochemistry but rather tensile properties of
bone. I haven't been able to find any information on the advantages or
disadvantages of bone storage with formalin vs alcohol and hope that
someone may be able to shed light on this issue or direct me to the
appropriate information site. Thanks in advance for your help.

 

Eric

 

 

 

 

 

 

 

Eric A. Breisch, Ph.D.

Clinical Anatomist

Dept. of Pathology

Rady Children's Hospital and Health Center

Associate Clinical Professor of Anatomy

Dept. of Surgery

UCSD School of Medicine 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information.  Any unauthorized review, use, disclosure or 
distribution is prohibited.  If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of the original 
message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Mucin staining

[Lee Wenk]

What type of mucin? What type of cell? What is it's origin?

PAS will stain neutral mucin.

Mucicarmine works better on epithelial acid mucins, rather than connective 
tissue acid mucins. But it will stain both sulfated and carboxylated acid 
mucins.


Alcian blue (pH 2.5) and colloidal iron work well on both epithelial and 
connective tissue acid mucins, and again on both sulfated and carboxylated 
acid mucins.


Alcian blue at pH 1.0 or lower) stains sulfated, but not carboxylated, acid 
mucins, both epithelial and connective tissue mucins.


High Iron Diamine (HID) stains sulfated acid mucins, of both epithelial and 
connective tissue origin, but not carboxylated mucins.


Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073


- Original Message - 
From: "Aprill Watanabe" 

To: 
Sent: Tuesday, March 03, 2009 12:22 PM
Subject: [Histonet] Mucin staining


Someone please refresh my memory.  I am looking for a mucin stain to stain
cell lines embedded in agarose then formalin fixed and embedded in paraffin.
I know mucicarmine might work, but I¹ve also heard about using alcian blue.
Can anyone point me in the right direction.
Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
main: 602-343-8822
Fax: 602-343-8840
awatan...@tgen.org
www.tgen.org

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] bone histology and storage

[Breisch, Eric]

Does anyone have a methodology for long term storage of bone? Formalin
vs alcohol? Orthopedic group here is wondering whether bone for research
purposes should be preferentially stored in alcohol. They aren't
interested in immunohistochemistry but rather tensile properties of
bone. I haven't been able to find any information on the advantages or
disadvantages of bone storage with formalin vs alcohol and hope that
someone may be able to shed light on this issue or direct me to the
appropriate information site. Thanks in advance for your help.

 

Eric

 

 

 

 

 

 

 

Eric A. Breisch, Ph.D.

Clinical Anatomist

Dept. of Pathology

Rady Children's Hospital and Health Center

Associate Clinical Professor of Anatomy

Dept. of Surgery

UCSD School of Medicine 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Cassette holders

[Martin, Gary]

Yes .. Hacker instruments www.hackerinstruments.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Spoon,
Victoria
Sent: Tuesday, March 03, 2009 12:17 PM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Cassette holders

Does anyone know where to purchase cassette holders?  Plastic or metal
holders for cassettes - 5 across and 4- 5 deep.

Thanks,


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] distilled water

[Bonner, Janet]

Try laying a glass slide down in the bottom of the waterbath after you swish 
the existing bubbles off the sides and bottom of the bathwatch the bubbles 
behave!  
   No  - I don't know why.
Janet
 
Janet L. Bonner, HTL (ASCP)
Pathology Laboratory



From: histonet-boun...@lists.utsouthwestern.edu on behalf of louise renton
Sent: Tue 3/3/2009 2:10 AM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] distilled water



Dear Kirsten

I've tried both, and find that tap water (presumably from all the aeration
in the pipes) makes bubbles on the side of the bath that are ALWAYS released
when you are cuting difficult section like lymph node or spleen.Of course
you can tap the water bath to release the bubbles, but it looks a bit
strange to be seen giving your unsuspecting bath a smart rap every so often.

So, my 2c (recesion adjusted) worth - use dist H2O.



On 3/2/09, kristen arvidson  wrote:
>
> Are most people using distilled water in the water baths?
>
>
>
> ___



===
The information contained in this message may be privileged and/or confidential
and protected from disclosure.  If the reader of this message is not the 
intended 
recipient or an employee or agent responsible for delivering this message to 
the 
intended recipient, you are hereby notified that any dissemination, 
distribution 
or copying of this communication is strictly prohibited.  If you have received 
this
communication in error, please notify the sender immediately by replying to 
this 
message and deleting the material from any computer.
===
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PA needed

[Marilyn . A . Weiss]

Kaiser Hospital in San Diego is in need of a PA to gross in large 
specimens, help with frozen sections and help with autopsies on a rotating 
system.Please contact Diane I Johnson at 619-528-5386 or by e-mail Diane. 
I .john...@kp.org

NOTICE TO RECIPIENT:  If you are not the intended recipient of this 
e-mail, you are prohibited from sharing, copying, or otherwise using or 
disclosing its contents.  If you have received this e-mail in error, 
please notify the sender immediately by reply e-mail and permanently 
delete this e-mail and any attachments without reading, forwarding or 
saving them.  Thank y
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Sulfated Alcian Blue

[Lee Wenk]

Sulfonate Alcian Blue (SAB) is not always as specific as you would like. 
Also, if you are using the newer alcian blue dye, it seems to have a 
different formulation than the old stuff that I have. The new alcian blue 
will not dissolve in alcohol, so the newer dye doesn't demonstrate the 
amyloid, while my old dye does.


Old deposits of amyloid do not have beta pleats at regular intervals. 
Therefore, the Congo red dye (CR) will still bind, but will not line up one 
right after the other | | | | |, but will be more random \  _  |  /.  When 
the CR dye are parallel to each other, they will show the apple green 
birefringence with the polarizing microscope. When the CR dye is randomly 
arranged, there will be no apple green birefringence.


The other time this happens is with very overfixed amyloid, such as months 
in NBF. Too many cross-links with NBF, so the CR dye can't bind right, so 
they are not in parallel. We also had this happen once when the autopsy 
tissues were fixed in B5 (mercury fixative, long time ago). The resident 
knew amyloid was an immunological problem, so put through tissue fixed in 
B5. Congo red did not birefringe. So we went back to the NBF stock bucket, 
submitted new tissue, and they all were wonderfully green birefringent.


The other problem with the SAB is that, if this is old amyloid, and the beta 
pleats are messed up, SAB depends somewhat on the beta pleats. Therefore, 
the green will be much paler in older amyloid than with newer amyoid. (The 
green is due to to the blue of alcian blue staining the amyloid, and the 
yellow of picric acid in the van Gieson also staining the amyloid, so blue 
and yellow make green.)


We just had a case of non-birefringing green Congo red at our hospital a 
couple of months ago, where it definitely looked by amyloid on the H&E, but 
there was no birefringence on the patient's CR (control was great - all 3 
times that we repeated the procedure). Ot wasn't a fixation problem, or a 
staining problem, but probably an "old amyloid" problem. Our resident Dr. 
Tom Fennel just gave a talk on it at our state histology's winter seminar 
Jan. 31, 2009. Here's what we did (all three):


1. View the Congo Red stained slides with a fluorescence microscope, such as 
in microbiology auramine-rhodamine stain for AFB. When hit with green light, 
the CR stained amyloid will fluoresce orange.
2. Use Crystal Violet or Methyl violet staining for amyloid. These depend 
upon the carboxyl  ions of the amyloid for binding, not the beta pleats. The 
amyloid should be violet, with the background blue/purple. However, it 
doesn't always demonstrate AA amyloid (some are low in surface carboxyl 
ions). So not always as sensitive as CR.
3. Use Thioflavin T or Thioflavin S for amyloid. Staining is (maybe) with 
the P component of amyloid, not the beta pleats. Use a fluorescence 
microsope, using blue light (FITC filters), and the amyloid will fluoresce 
yellow. However, other things also fluoresce yellow, such as fibrinoid 
material, JG granules, sometimes elastin, etc. So not as specific as CR.


By knowing your histology and knowing where in the tissue you are thinking 
that there are amyloid deposits, the above three alternatives are a nice 
addition, for when Congo red is not demonstrating the apple green 
birefringence.


Since in our recent case, 3 out of 4 stains demonstrated amyloid (CR 
fluorescent, Crystal violet and TFT were all positive, while CR 
birefringence was negative), it was diagnosed as amyloid, with, I think, a 
note that older deposits of amyloid sometimes demonstrate no birefringence 
with CR.


If this person needs help with find staining procedures for CV/MV or 
TFT/TFS, could someone email them? I'm on vacation, and don't have access to 
my home or work computer.


Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073


- Original Message - 
From: "Nathanial nauss" 

To: 
Sent: Friday, February 27, 2009 10:42 AM
Subject: [Histonet] Sulfated Alcian Blue


I need some help, has any one used the sulfated alcian blue to stain for 
amyloid. We have a case that looks like it should be positive but it is not 
staining with the Congo Red. Any help would be great.

Nathaniel
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Storage of muscles

[Adrienne Anderson]

Hello,
We are having problems with artifact after our muscles have been stored for 
long periods of time. Can anyone tell me how they store their muscle specimens 
for long-term?
 
Thank you,
Adrienne Anderson
Duke University Health System
rennie1...@yahoo.com
 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mucin staining

[Aprill Watanabe]

Someone please refresh my memory.  I am looking for a mucin stain to stain
cell lines embedded in agarose then formalin fixed and embedded in paraffin.
I know mucicarmine might work, but I¹ve also heard about using alcian blue.
Can anyone point me in the right direction.
Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
main: 602-343-8822
Fax: 602-343-8840
awatan...@tgen.org
www.tgen.org

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cassette holders

[Spoon, Victoria]

Does anyone know where to purchase cassette holders?  Plastic or metal
holders for cassettes - 5 across and 4- 5 deep.

Thanks,


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Biocare intelliPATH

[godsgalnow]

Excellent

Roxanne


-Original Message-
From: Jackie M O'Connor 
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Sent: Tue, 3 Mar 2009 10:57 am
Subject: [Histonet] Biocare intelliPATH



Soliciting opinions on this IHC stainer.  Good or bad.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histonet Digest, Vol 64, Issue 4

[Marilyn . A . Weiss]

In regard to tracking OR specimens, we use a "handling transmittal " form. 
On it is the patient information, PF number, doctor, courier, who it was 
rc'd by and  the specimen .
The OR fills it out and whoever receives the specimens will check the 
paperwork against the name on the container .

NOTICE TO RECIPIENT:  If you are not the intended recipient of this 
e-mail, you are prohibited from sharing, copying, or otherwise using or 
disclosing its contents.  If you have received this e-mail in error, 
please notify the sender immediately by reply e-mail and permanently 
delete this e-mail and any attachments without reading, forwarding or 
saving them.  Thank you.




histonet-requ...@lists.utsouthwestern.edu 
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/03/2009 10:01 AM
Please respond to
histonet@lists.utsouthwestern.edu


To
histonet@lists.utsouthwestern.edu
cc

Subject
Histonet Digest, Vol 64, Issue 4






Send Histonet mailing list submissions to
 histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
 histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
 histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Re: Re: Mohs technique (Kim Tournear)
   2. Re: Section thickness (Vanessa J. Phelan)
   3. Hologic Thin Prep 2000 Imaging Sytem
  (julie_schoenb...@bradycorp.com)
   4. Biocare intelliPATH (Jackie M O'Connor)
   5. RE: Biocare intelliPATH (Blazek, Linda)
   6. Re: specimen tracking from the OR (Matthew Lunetta)
   7. Temp Position In San Diego Ca. (James Watson)
   8. Manager's question (Terri  Braud)


--

Message: 1
Date: Tue, 3 Mar 2009 07:48:28 -0800 (PST)
From: Kim Tournear 
Subject: Re: [Histonet] Re: Mohs technique
To: Histonet 
Message-ID: <10226.53099...@web54203.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

The derm office I workd in found a neat little gadget called a 
"cryo-embedder". It is sold by Belaire Instruments and works great. Better 
than trying to use cryomolds, slides, etc...



~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson,  AZ
~Don't let your life end before it begins~
OU Rocks

--- On Wed, 2/25/09, whitmorel  wrote:

From: whitmorel 
Subject: [Histonet] Re: Mohs technique
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, February 25, 2009, 12:21 PM

First of all, there is no apostrophe in Mohs. Sorry, being picky is a 
necessity
for a Mohs tech.  How are you mounting your tissue?  Are you using a glass 
slide
to mount the tissue?  There are several different method out there for 
mounting
tissue so you can be sure the entire epidermis is completely down and air
bubbles are out of the tissue.  You might have your surgeon contact the 
Mohs
College and see about getting a trainer to come and work with you.  With 
your
background, the 2 days the College suggests would work great. The other
alternative is to go and visit a trainer.  If you go onto the website
www.mohscollege.org you can find a list of Mohs histotech trainers. 

Lynn Whitmore HT(ASCP)
Mohs Histotechnology Trainer

-
>--
>
>Message: 17
>Date: Wed, 25 Feb 2009 06:56:12 -0800 (PST)
>From: Steven Coakley 
>Subject: [Histonet] Moh's techniques
>To: Histonet@lists.utsouthwestern.edu
>Message-ID: <164204.1533...@web38206.mail.mud.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>Good morning all,
> 
>I'm learning a new way to do Moh's.  Much more relaxed compared to
how I did them years ago with a Pathologist looking over my shoulder while 
I
attempted to cryosection 12, 3,9,12 o'clock boarders, stain them by hand 
and
the Dermatologist wanting the results "yesterday".  I'd
like  to get some ideas as to techniques Moh's Techs are using out there
that work well in assuring that one gets the entire skin edge.  I'd also
Like to shadow in any fairly local Moh's labs in the So.WI or No. Ill. 
area.
> 
>Thanks everyone,
> 
>Steve
> 
>
>
> 
>
>--
>
>M

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 

--

Message: 2
Date: Tue, 3 Mar 2009 10:53:51 -0500
From: "Vanessa J. Phelan" 
Subject: Re: [Histonet] Section thickness
To: , 
Message-ID: 
Content-Type: text/plain; charset="ISO-8859-1"

Ammunition...no, was more just inquiring what the what the average would 
be
and if there was any specific reason for thinner sections other that just
preference.

Thanks

Vanessa



On 3/3/09 9:37 AM, "Rene J

[Histonet] Cresyl violet problems

[Walters, Katherine S]

To the "brainy" histo-people,

I have a student doing a cresyl violet stain on some frozen (40um) brain
sections.  He has been doing this routinely for a couple of months, but
his last run looks very odd.  There appears to be an area on each
section where there is no staining.  At first glance I thought it was a
fixation problem, but the unstained portion of the tissue is in a
different area as you move from slice to slice (these are serial
sections),   He stains in a Copeland jar and assures me that his stain
is well homogenized.  Could this be some kind of moisture problem?

Thanks for your thoughts.


Katherine Walters
Histology Director
Central Microscopy Research Facilities
85 Eckstein Medical Research Building
University of Iowa
Iowa City, Iowa 52242-1101

katherine-walt...@uiowa.edu
www.uiowa.edu/~cemrf







Notice: This UI Health Care e-mail (including attachments) is covered by the 
Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and 
may be legally privileged.  If you are not the intended recipient, you are 
hereby notified that any retention, dissemination, distribution, or copying of 
this communication is strictly prohibited.  Please reply to the sender that you 
have received the message in error, then delete it.  Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mercuric chloride SOP for Golgi

[Bob Nienhuis]

Anyone have a handy SOP for safe handling and disposal of
Mercuric chloride as used in Golgi-Cox staining?

This is for a VA  research facility. Our safety guys think ours in
inadequate.

Bob Nienhuis
VA / UCLA Medical Center
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Biocare intelliPATH

[Pat Laurie]

We just got an IntelliPath installed.  We are currently evaluating it Vs.
Ventana's Benchmark.  Currently, I have nothing but good things to say about
it.  The good things are

Cold spot for reagents unstable at room temperature
 •On-board reagent mixing vials
 •50 slides split into 5 simultaneous runs
Can run on a first in, first out basis, multiple 3 hour runs mean 150 slides
finished in 9 hours
Multiplex staining, (using the Biocare products) mean that dual or even more
stains take the same time as a single stain,
One of the best things is OPEN abilities.  Can use reagents from any source
– Dako, Leica Lab Vision and Ventana, etc
Full ability to modify detection protocol.  Retrieval, buffering, and
chromagen ancillaries can be from any possible kit or individual reagent.
And I must concur, the Installation and support is excellent.  If you need
any further info from me, I would be happy to help.

-- 
Patrick Laurie HT(ASCP)QIHC
Cellnetix Seattle, Wa



On Tue, Mar 3, 2009 at 7:57 AM, Jackie M O'Connor <
Jackie.O'con...@abbott.com > wrote:

> Soliciting opinions on this IHC stainer.  Good or bad.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Manager's question

[Terri Braud]

I need some histonet help, please.
If you are a manager and if you are being asked to manage to "units per 
productive manhour" where a unit is one billed test, would you mind sharing 
your budgeted target?
Also, if you have this information, please let me know what services your lab 
offers...Histology, IHC, Cytology?
I've been asked to manage to this figure before, but we always had benchmarked 
figures to go by.
Thanks in advance, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital and Medical Center
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3676 phone
(215) 938-3689 fax


-



CONFIDENTIALITY NOTICE:

This E-Mail is intended only for the use of the individual or entity to which
it was sent. It may contain information that is privileged and/or confidential,
and the use or disclosure of such information may also be restricted under 
applicable
federal and state law. If you received this communication in error, please do 
not
distribute any part of it or retain any copies, and delete the original E-Mail.
Please notify the sender of any error by E-Mail.

Thank you for your cooperation.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Temp Position In San Diego Ca.

[James Watson]

Would you like to spend the summer in Sunny San Diego Ca.?

 

Job Description

GNF is currently seeking a temporary Scientific Associate to join the
Histology group (End of June 2009 through beginning of October 2009).

 

 Job Summary

Performs routine, special staining and complex procedures necessary in
preparing specimens of animal tissue in a research environment.

Qualifications

Associate's degree in a biological science or completion of a NAACLS
accredited School of Histotechnology is required.  

Having the American Society of Clinical Pathologist (ASCP) certification
as a Histology Technician is required. 

Applicants must demonstrate the ability to perform the essential
functions of the job as outlined in the position description. 

Experience

Applicant should have at least 2 years of experience in animal
techniques, a wide variety of manual histochemical and enzymatic
staining, automated and manual immunohistochemistry.

Essential Functions

1.  Identifies significant tissue elements microscopically to
determine quality of staining. 
2.  Necropsy, fix, trim, process, and embed animal tissue for
paraffin and frozen sections.
3.  Performs microtomy on rotary microtome, cryostat.
4.  Prepares dyes and solutions in order to perform special or
complex procedures. 
5.   Have the background to do basic histochemical stains and
enzymatic histochemical stains. Have the background in
Immunohistochemical staining and other advanced histological procedures.

6.  Maintains lab work area by performing preventative maintenance
on instruments and equipment and keeping the work area clean and
orderly.
7.  Operate Slide scanning instrumentation.

The Genomics Institute of the Novartis Research Foundation (GNF),
located in the Torrey Pines area of San Diego, CA, is funded by the
Novartis Research Foundation and dedicated to the development and
application of new methods and techniques for genome-wide biological
discovery and biomedical research.  GNF provides a unique and
challenging opportunity to combine exploratory biomedical research with
pharmaceutical drug development in a highly interactive,
multidisciplinary environment and state-of-the-art facilities.  GNF
offers excellent compensation and a great benefits package.  Visit our
website at www.gnf.org EOE

 

Please submit your CV and any supporting documents to:

 

Genomics Institute of the Novartis Research Foundation


Job Code: JW03-003


10675 John Jay Hopkins Drive

San Diego, CA 92121

 

Fax: 858/812-1670, or submit online to j...@gnf.org

(subject line must include JW03-003)

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: specimen tracking from the OR

[Matthew Lunetta]

We too are a small hospital. And like Terri there is a book in the OP area that 
the specimens are logged into when dropped off and picked up. It is a standard 
Chain-of-Custody (COC) protocol. We have recently added a new process as we too 
had a sample missplaced when the Histology Lab was closed. Now after hours 
tissue is brought to the General lab and a COC log is filled out with the RN 
dripping off the specimen and the Lab Assistant accepting the specimen.  To 
copy Terri, Crude and time consuming, yesaccurate, too.

Regards,
Matt Lunetta HT, (ASCP)
Longmont United Hospital


Message: 2
Date: Mon, 2 Mar 2009 13:28:22 -0500
From: "Terri  Braud" 
Subject: [Histonet] RE: specimen tracking from the OR
To: 
Message-ID:

Content-Type: text/plain;   charset="iso-8859-1"

We are a small hospital lab, too.  There is a log book in the OR, where 
specimens are placed for Histo pick up.  The circulating nurse puts a Patient 
sticker and handwrites all specimens removed from the patient, regardless of 
what they are, and whether or not they have been walked down, tubed, or waiting 
pickup.  We make sure that all specimens we remove are listed in the book, then 
sign/date/time for them.  Any specimen left for us and not in the book, or vice 
versa, we bring to the attention of the OR control desk.  If we make a run and 
there are no specimens to pick up, then we still sign and date/time the log 
book as "no specimens".   Any specimens brought to the department are checked 
before accepting.  Crude and time consuming, yesaccurate, too.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital and Medical Center
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3676 phone
(215) 938-3689 fax

  6. log book (Kathy Gorham)
 
Message: 6
Date: Sun, 01 Mar 2009 17:15:47 -0800
From: "Kathy Gorham" 
Subject: [Histonet] log book
Good Monday Morning,  We had a serious incident Friday with O.R.  My aide went 
down to get the specimens from O.R. about 9am. (which were left overs from the 
night before).  She did not stamp in the specimens before she left.  When I had 
time to stamp them in and record them in the log book I discovered that the 
colon was not there.  Two other specimens from that patient where in the bag 
but no colon.  So I went down to O.R. to see where it was.  Of course no one 
knows what happened to the colon.  The doctors are furious by all means.  Now 
the O.R. thinks the path lab screwed up.  So my questions is how do others log 
in the specimens as they come into the lab.  We have 2 couriers that brings 
specimens when we are not in the lab from other hospitals.  How do you make 
sure that whom ever brings the specimens actually brings the ones they say they 
do?  Do you have a log book that every specimen that is brought into the lab is 
written down by the person who brings it in?  Right n
ow we have a log book but it is written in as we are accessing  the specimens.  
So the specimens may have been there overnight. We are a very small lab and we 
do almost everything by hand including writing in the log book.  Someday we 
want to be able to scan by bar codes but right now we can not do that. Thanks 
for any help you can give me. 
Kathy Gorham, H.T.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Biocare intelliPATH

[Blazek, Linda]

Jackie,
I have Biocare's intelliPath and like it very much.  It is an open system so I 
have the option to use any reagents I choose.  You can continuously add stains 
through out the day even if you have a protocol running.  Instillation, support 
and service has been great.  
You can contact me any time if you would like any additional information.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M 
O'Connor
Sent: Tuesday, March 03, 2009 10:58 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Biocare intelliPATH

Soliciting opinions on this IHC stainer.  Good or bad.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Biocare intelliPATH

[Jackie M O'Connor]

Soliciting opinions on this IHC stainer.  Good or bad.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Hologic Thin Prep 2000 Imaging Sytem

[Julie_Schoenborn]

Does anyone have a copy of the ocr-a font so lab can print slide labels 
with appropriate readable text for this this system?

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Section thickness

[Vanessa J. Phelan]

Ammunition...no, was more just inquiring what the what the average would be
and if there was any specific reason for thinner sections other that just
preference.

Thanks

Vanessa



On 3/3/09 9:37 AM, "Rene J Buesa"  wrote:

> Vanessa:
> Lymph nodes for cellular details (special request) = 3 µm
> H&E and all other special procedures = 5 µm
> Sections for bone marrow and liver reticulum stain = 7 µm
> Brain and central nervous system = 10 µm
>  
> Now a question, why do you want to know this? To have "ammunition" to
> challenge what is done in your new lab? Not a wise move. I don't think that
> they would mind if you cut thinner, but they will mind if you start bringing
> this issue about. Let your thinner sections "speak for themselves". It will
> get the moment that "by example" your way will prevail.
> René J.
> 
> --- On Tue, 3/3/09, Vanessa J. Phelan  wrote:
>> From: Vanessa J. Phelan 
>> Subject: [Histonet] Section thickness
>> To: histonet@lists.utsouthwestern.edu
>> Date: Tuesday, March 3, 2009, 9:14 AM
>> 
>> Hi Guys,
>> 
>> Just wondering what thickness you cut sections at? I was always used to
>> cutting at 2-3 microns in my last lab, however in my new place they are
>> cutting at 6 microns (for both H & Es and IHC), which seems to me as really
>> quite thick! What would be the average cutting thickness?
>> 
>> Thanks a mill,
>> 
>> Vanessa 
>> 
>> 
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Re: Mohs technique

[Kim Tournear]

The derm office I workd in found a neat little gadget called a "cryo-embedder". 
It is sold by Belaire Instruments and works great. Better than trying to use 
cryomolds, slides, etc...



~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson,  AZ
~Don't let your life end before it begins~
OU Rocks

--- On Wed, 2/25/09, whitmorel  wrote:

From: whitmorel 
Subject: [Histonet] Re: Mohs technique
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, February 25, 2009, 12:21 PM

First of all, there is no apostrophe in Mohs. Sorry, being picky is a necessity
for a Mohs tech.  How are you mounting your tissue?  Are you using a glass slide
to mount the tissue?  There are several different method out there for mounting
tissue so you can be sure the entire epidermis is completely down and air
bubbles are out of the tissue.  You might have your surgeon contact the Mohs
College and see about getting a trainer to come and work with you.  With your
background, the 2 days the College suggests would work great. The other
alternative is to go and visit a trainer.  If you go onto the website
www.mohscollege.org you can find a list of Mohs histotech trainers.  

Lynn Whitmore HT(ASCP)
Mohs Histotechnology Trainer

-
>--
>
>Message: 17
>Date: Wed, 25 Feb 2009 06:56:12 -0800 (PST)
>From: Steven Coakley 
>Subject: [Histonet] Moh's techniques
>To: Histonet@lists.utsouthwestern.edu
>Message-ID: <164204.1533...@web38206.mail.mud.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>Good morning all,
> 
>I'm learning a new way to do Moh's.  Much more relaxed compared to
how I did them years ago with a Pathologist looking over my shoulder while I
attempted to cryosection 12, 3,9,12 o'clock boarders, stain them by hand and
the Dermatologist wanting the results "yesterday".  I'd
like  to get some ideas as to techniques Moh's Techs are using out there
that work well in assuring that one gets the entire skin edge.  I'd also
Like to shadow in any fairly local Moh's labs in the So.WI or No. Ill. area.
> 
>Thanks everyone,
> 
>Steve
> 
>
>
>  
>
>--
>
>M

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Section thickness

[Peter Carroll]

> Just wondering what thickness you cut sections at?

3 microns for all paraffin...


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Section thickness

[Houston, Ronald]

3 microns, but 2 micron for lymphoid tissues and renal biopsies

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan
Shivers
Sent: Tuesday, March 03, 2009 10:17 AM
To: Vanessa J. Phelan; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Section thickness

4 um here.

Jan Shivers
UMN VDL

- Original Message - 
From: "Vanessa J. Phelan" 
To: 
Sent: Tuesday, March 03, 2009 8:14 AM
Subject: [Histonet] Section thickness


> Hi Guys,
>
> Just wondering what thickness you cut sections at? I was always used
to
> cutting at 2-3 microns in my last lab, however in my new place they
are
> cutting at 6 microns (for both H & Es and IHC), which seems to me as 
> really
> quite thick! What would be the average cutting thickness?
>
> Thanks a mill,
>
> Vanessa
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
intended recipient (or authorized to receive information for the
intended recipient), you are hereby notified that any review, use,
disclosure, distribution, copying, printing, or action taken in
reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Section thickness

[Jan Shivers]

4 um here.

Jan Shivers
UMN VDL

- Original Message - 
From: "Vanessa J. Phelan" 

To: 
Sent: Tuesday, March 03, 2009 8:14 AM
Subject: [Histonet] Section thickness



Hi Guys,

Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in my last lab, however in my new place they are
cutting at 6 microns (for both H & Es and IHC), which seems to me as 
really

quite thick! What would be the average cutting thickness?

Thanks a mill,

Vanessa


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Section thickness

[Merced Leiker]

...I just want to add, I could see it being a problem only in the sense 
that thicker sections tend to give more background in your 
immunostaining...however, you could conversely get greater signal strength 
as well...


--On Tuesday, March 03, 2009 9:14 AM -0500 "Vanessa J. Phelan" 
 wrote:



Hi Guys,

Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in my last lab, however in my new place they are
cutting at 6 microns (for both H & Es and IHC), which seems to me as
really quite thick! What would be the average cutting thickness?

Thanks a mill,

Vanessa


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvienienced!


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Section thickness

[Merced Leiker]

I've read that 4-5um is "average", and that's what I typically go by for 
paraffins, but then it could depend on tissue type as Rene recommended.  I 
end up having to cut frozens at 8-10um because for some reason it's very 
difficult to cut any thinner on the cyrostat I use (an old Vibratome 
model).  But doesn't seem to matter for our applications - we do mostly 
immunofluorescence & some H&E.


Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.

--On Tuesday, March 03, 2009 9:14 AM -0500 "Vanessa J. Phelan" 
 wrote:



Hi Guys,

Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in my last lab, however in my new place they are
cutting at 6 microns (for both H & Es and IHC), which seems to me as
really quite thick! What would be the average cutting thickness?

Thanks a mill,

Vanessa


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvienienced!


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Section thickness

[Rene J Buesa]

Vanessa:
Lymph nodes for cellular details (special request) = 3 µm
H&E and all other special procedures = 5 µm
Sections for bone marrow and liver reticulum stain = 7 µm
Brain and central nervous system = 10 µm
 
Now a question, why do you want to know this? To have "ammunition" to challenge 
what is done in your new lab? Not a wise move. I don't think that they would 
mind if you cut thinner, but they will mind if you start bringing this issue 
about. Let your thinner sections "speak for themselves". It will get the moment 
that "by example" your way will prevail.
René J.

--- On Tue, 3/3/09, Vanessa J. Phelan  wrote:

From: Vanessa J. Phelan 
Subject: [Histonet] Section thickness
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 3, 2009, 9:14 AM

Hi Guys,

Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in my last lab, however in my new place they are
cutting at 6 microns (for both H & Es and IHC), which seems to me as really
quite thick! What would be the average cutting thickness?

Thanks a mill,

Vanessa 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Manual for Nikon 300 diaphot

[Stacey Barrick]

Hi Janet, 
 
I have sent you an email with the manual attached as a pdf. 
 
Stacey 
--- On Mon, 3/2/09, Dertien, Janet  wrote:

From: Dertien, Janet 
Subject: [Histonet] Manual for Nikon 300 diaphot
To: histonet@lists.utsouthwestern.edu
Date: Monday, March 2, 2009, 4:45 PM

 

Hello,

 

Does anyone have a manual for a Nikon Diaphot 300?

I found a previous similar request by Necat Yilmaz, and a positive
response by Stacey Barick. 

Your help would be much appreciated!

 

Thanks,

Janet

Janet Dertien

Faculty Associate

Texas Tech University Health Science Center

Pharmacology & Neuroscience

3601 4th Street / STOP 6592

Lubbock, TX 79430

806-743-2425 x236

806-743-2744

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Section thickness

[Vanessa J. Phelan]

Hi Guys,

Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in my last lab, however in my new place they are
cutting at 6 microns (for both H & Es and IHC), which seems to me as really
quite thick! What would be the average cutting thickness?

Thanks a mill,

Vanessa 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histotech position

[Sharon Campbell]

Good morning everyone!

We have a position available in the Charlotte, NC for an ASCP certified
Histotech (HT/or HTL). The hours are from 11AM-7PM. This person will
need to have IHC/ISH experience as well as be able to gross (process)
small specimens, mostly derms and GYN (ECC's, cervical Bx's). Please
send resume's to shar...@celligent.net or fax them to 704-549-0559 attn.
Sharon Campbell.

Thank you

 

Sharon Campbell, HTL(ASCP)CM, BSBM

Histology Supervisor

Celligent Diagnostics, LLC

Formerly Pathology Associates Services

101 East W.T. Harris Blvd, Suite 1212

Charlotte, NC  28262

800-524-6779 ext. 104

704-970-3304 Direct Line

shar...@celligent.net

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet