Re: [Histonet] problem eyes

[Anne van Binsbergen]

do a search on the histonet archive
i posted a method quite a few years back
processed and cut eyes (human and piggy) on a daily basis for almost 10
years
go find it - it may help

patience, determination, thicker sections, 2 x pre-float out first on dist
water and then very dilute alcohol to manually tease out the crinkles, then
a gentle float on low ttemp water bath
if its too hot and the sections are too thin they will shatter on the warm
water
plenty of wax support in the block (use a bigger mould when you embed)

i am on vacation and saw this post by chance

unable to reply in too much detail

good luck

Annie (visiting in Africa)

2009/3/4 Perry, Margaret 

> Please help!  We have been trying to cut whole eyes from a pig.  They need
> to be nice enough for a publication.  We are having the devil of a time
> because no matter what we do there are wrinkles.  If we turn the waterbath
> up to stretch things out the retina detaches.  Do any of you have
> suggestions?  We uses Surgipath  EM400 for embedding paraffin and and their
> infiltration medium.  Is this to soft? The lowest we can turn our waterbath
> is 38 degrees C.
>
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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[Histonet] Natural Killer Cell antibody

[Kim O'Sullivan]

Hi,

Does anyone out there know if there is a monoclonal anti human NK cell antibody 
that is exclusive, ie does not also mark CD8 T cells etc?

Kim 

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[Histonet] AXOn staining

[TF]

Re: 
Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think 
Tuj1 is restricted to immature neurons inside the brain ! though it stains well 
with retinal ganglion cell

I am also seeking for a nice marker for axon ...both normal and 
regenerating...GAP-43 is one choice for regenerating axons...but a bit focused 
on the growth cone, and the staining is not strong on transporting proteins - 
due to the tiny amount? I am not sure how NF-200 or NF-96/160 work

ANyone can suggest better markers for axon IHC staining? both normal and 
regenerating.

___
Axons stain nicely with beta 3 tubuline (TUJ). We've used the following
antibodies
Monoclonal anti-beta-tubulin isotype III from Sigma  1/1000  DAKO envision
(révélation PAL or HRP) (ref : T8660)
or
Polyclonal  (rabbit)  antibody against neuronal class III beta-tubulin from
covance  research  commercialise  par  BABCO  Berkeley  antibody company au
1/1000 DAKO envision (révélation PAL or HRP) (ref : PRB-435P)

We  used  paraffin  slices  of  PFA  fixed rat embryos and adult rat brain,
microwaving in citrate buffer. These antibodies also work nicely with IF

Good luck!
Nancy Walker
Molecular Biology Scientist

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.wal...@sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001



2009-03-05 



TF 
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[Histonet] colon cancer lymph nodes

[Steve Eagle]

An additional step that often is helpful is to submit pericolonic adipose 
tissue with vessels... frequently you can find microscopic lymph nodes/lymphoid 
aggregates.


  

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RE: [Histonet] problem eyes

[Tony Henwood]

Doesn't help with the wrinkles in OLD large Histotechs.

Sorry, couldn't resist

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula 
Sicurello
Sent: Thursday, 5 March 2009 8:23 AM
To: histonet@lists.utsouthwestern.edu; MargaretPerry; rjbu...@yahoo.com
Subject: Re: [Histonet] problem eyes



Really?  Liquid laundry soap?  Does this help with all wrinkles in large 
specimens?


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Wed, 3/4/09, Rene J Buesa  wrote:

> From: Rene J Buesa 
> Subject: Re: [Histonet] problem eyes
> To: "histonet@lists.utsouthwestern.edu" 
> , "Perry, Margaret" 
> 
> Date: Wednesday, March 4, 2009, 9:14 PM
> Add a few drops (4-5) of liquid
> detergent, but not dishwasher detergent (because it will
> dissolve the paraffin).
> rené J.
> 
> --- On Wed, 3/4/09, Perry, Margaret 
> wrote:
> 
> From: Perry, Margaret 
> Subject: [Histonet] problem eyes
> To: "histonet@lists.utsouthwestern.edu"
> 
> Date: Wednesday, March 4, 2009, 4:04 PM
> 
> Please help!  We have been trying to cut whole eyes
> from a pig.  They need to be
> nice enough for a publication.  We are having the
> devil of a time because no
> matter what we do there are wrinkles.  If we turn the waterbath up to 
> stretch things out the retina detaches.  Do any of you have
> suggestions?  We uses
> Surgipath  EM400 for embedding paraffin and and their
> infiltration medium.  Is
> this to soft? The lowest we can turn our waterbath is 38
> degrees C.
> 
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007
> 
> ___
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
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> 


  


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[Histonet] Autotechnicon model 2A

[Va Paula Sicurello]

Hi All,

Does anybody have an owner's manual to an Autotechnicon 2A?  I've inherited 
this 42 year old beasty and can't remember how to work the delay timer.

Yes, I am old enough to have used one of these R2D2 units in the past.

If you can help me, I'd be much obliged.

Paula  :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


  


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Re: [Histonet] Water bubble on slides

[Merced Leiker]

Hi Vanessa,

I have read in at least one online protocol and have also been advised by 
our excellent and experienced (former) histotech who used to be here at UB 
NOT to warm the slides without air-drying first.  We typically air-dry the 
slides, yes, standing up, overnight, before warming them.


Merced

--On Wednesday, March 04, 2009 4:35 PM -0500 "Vanessa J. Phelan" 
 wrote:



Hi everyone,

Quick question, I am finding after cutting a lot of slides for H&Es onto
VWR Superfrost Plus slides (these are the only slides we have at the
moment) that when I lift sections from the water bath ( of distilled
water) a water bubble stays under the sections on the slide. When I lie
them flat on the hot plate to dry the water bubble is tending to distort
the tissue by escaping out through he tissue, eventually. I have not
experienced this before, the water has ran off the slide no problem.
Would it be the slides that are the problem or anything I might need to
add to the water?

I have just ordered a histology oven, this may be the solution...drying
the slides standing up!

Vanessa


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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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RE: [Histonet] vector blue- xylene soluble- any alternatives

[Patsy Ruegg]

If I am not mistaken vectamount is an aqueous mountant, dpx is
xylene/toluene based and requires going thru alcohols and xylene to mount.
One thing I do and you can try it, is airdry the slides and the use
permanent mounting media without going thru alcohols and xylene, keep in
mind there is a little solvent in the media so it may have an effect.  I do
this with Fast Red substrate chromogen for AP have not tried it on vector
blue.  The other option is to use an aqueous mountant and seal the coverslip
edges with clear nail polish.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anjan kumar
Sent: Wednesday, March 04, 2009 12:05 PM
To: histonet-reque...@lists.utsouthwestern.edu; triple immunohistochem
Subject: [Histonet] vector blue- xylene soluble- any alternatives


hello everyone,

i have a problem which i dont know how to tackle, the
problem is that i have purchased vector blue as a chromogen and it is xylene
soluble can anyone suggest me how to avoid this as funds are low i cant
purchase xylene substitutes. 

 

second question been, ...sorry this has been a addition ..

what is the difference between the DPX mountant and other permanent mounting
media like vectamount. kindly answer these questions...

 

regards,

Anjan Kumar

Junior research scientist

Veterinary Pathology

Madras veterinary college.

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RE: [Histonet] problem eyes

[Connolly, Brett M]

My suggestion would be to follow the procedure of the old ocular guru
from the AFIP, Peter Emanuale. He taught me how the section human and
rabbit eyes. It involves using 2 water baths, one of which is at room
temp... and you should not use charged/coated slides.

Check our his chapter on ocular histotechnology in the 1992 edition of
the AFIP Lab Methods manual (it has step by step illustrations)

Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
e-mail. brett_conno...@merck.com
   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry,
Margaret
Sent: Wednesday, March 04, 2009 4:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] problem eyes

Please help!  We have been trying to cut whole eyes from a pig.  They
need to be nice enough for a publication.  We are having the devil of a
time because no matter what we do there are wrinkles.  If we turn the
waterbath up to stretch things out the retina detaches.  Do any of you
have suggestions?  We uses Surgipath  EM400 for embedding paraffin and
and their infiltration medium.  Is this to soft? The lowest we can turn
our waterbath is 38 degrees C.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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[Histonet] Water bubble on slides

[Vanessa J. Phelan]

Hi everyone,

Quick question, I am finding after cutting a lot of slides for H&Es onto VWR
Superfrost Plus slides (these are the only slides we have at the moment)
that when I lift sections from the water bath ( of distilled water) a water
bubble stays under the sections on the slide. When I lie them flat on the
hot plate to dry the water bubble is tending to distort the tissue by
escaping out through he tissue, eventually. I have not experienced this
before, the water has ran off the slide no problem. Would it be the slides
that are the problem or anything I might need to add to the water?

I have just ordered a histology oven, this may be the solution...drying the
slides standing up!

Vanessa


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Re: [Histonet] problem eyes

[Merced Leiker]

Good question! I'd like to know that, too! But with any size specimen, 
please!


--On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello 
 wrote:




Really?  Liquid laundry soap?  Does this help with all wrinkles in large
specimens?


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Wed, 3/4/09, Rene J Buesa  wrote:


From: Rene J Buesa 
Subject: Re: [Histonet] problem eyes
To: "histonet@lists.utsouthwestern.edu"
, "Perry, Margaret"
 Date: Wednesday, March 4, 2009, 9:14 PM
Add a few drops (4-5) of liquid
detergent, but not dishwasher detergent (because it will
dissolve the paraffin).
rené J.

--- On Wed, 3/4/09, Perry, Margaret 
wrote:

From: Perry, Margaret 
Subject: [Histonet] problem eyes
To: "histonet@lists.utsouthwestern.edu"

Date: Wednesday, March 4, 2009, 4:04 PM

Please help!  We have been trying to cut whole eyes
from a pig.  They need to be
nice enough for a publication.  We are having the
devil of a time because no
matter what we do there are wrinkles.  If we turn the
waterbath up to stretch
things out the retina detaches.  Do any of you have
suggestions?  We uses
Surgipath  EM400 for embedding paraffin and and their
infiltration medium.  Is
this to soft? The lowest we can turn our waterbath is 38
degrees C.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Re: [Histonet] problem eyes

[Rene J Buesa]

Yes, really with any type of large (and small) sections. The liquid soap will 
reduce the surface tension of water (more than heat) allowing the sections to 
expand easily. Try it..
René J.

--- On Wed, 3/4/09, Va Paula Sicurello  wrote:

From: Va Paula Sicurello 
Subject: Re: [Histonet] problem eyes
To: "histonet@lists.utsouthwestern.edu" , 
"MargaretPerry" , rjbu...@yahoo.com
Date: Wednesday, March 4, 2009, 4:23 PM

Really?  Liquid laundry soap?  Does this help with all wrinkles in large
specimens?


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Wed, 3/4/09, Rene J Buesa  wrote:

> From: Rene J Buesa 
> Subject: Re: [Histonet] problem eyes
> To: "histonet@lists.utsouthwestern.edu"
, "Perry, Margaret"

> Date: Wednesday, March 4, 2009, 9:14 PM
> Add a few drops (4-5) of liquid
> detergent, but not dishwasher detergent (because it will
> dissolve the paraffin).
> rené J.
> 
> --- On Wed, 3/4/09, Perry, Margaret 
> wrote:
> 
> From: Perry, Margaret 
> Subject: [Histonet] problem eyes
> To: "histonet@lists.utsouthwestern.edu"
> 
> Date: Wednesday, March 4, 2009, 4:04 PM
> 
> Please help!  We have been trying to cut whole eyes
> from a pig.  They need to be
> nice enough for a publication.  We are having the
> devil of a time because no
> matter what we do there are wrinkles.  If we turn the
> waterbath up to stretch
> things out the retina detaches.  Do any of you have
> suggestions?  We uses
> Surgipath  EM400 for embedding paraffin and and their
> infiltration medium.  Is
> this to soft? The lowest we can turn our waterbath is 38
> degrees C.
> 
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> ___
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> 








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Re: [Histonet] problem eyes

[Va Paula Sicurello]

Really?  Liquid laundry soap?  Does this help with all wrinkles in large 
specimens?


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Wed, 3/4/09, Rene J Buesa  wrote:

> From: Rene J Buesa 
> Subject: Re: [Histonet] problem eyes
> To: "histonet@lists.utsouthwestern.edu" , 
> "Perry, Margaret" 
> Date: Wednesday, March 4, 2009, 9:14 PM
> Add a few drops (4-5) of liquid
> detergent, but not dishwasher detergent (because it will
> dissolve the paraffin).
> rené J.
> 
> --- On Wed, 3/4/09, Perry, Margaret 
> wrote:
> 
> From: Perry, Margaret 
> Subject: [Histonet] problem eyes
> To: "histonet@lists.utsouthwestern.edu"
> 
> Date: Wednesday, March 4, 2009, 4:04 PM
> 
> Please help!  We have been trying to cut whole eyes
> from a pig.  They need to be
> nice enough for a publication.  We are having the
> devil of a time because no
> matter what we do there are wrinkles.  If we turn the
> waterbath up to stretch
> things out the retina detaches.  Do any of you have
> suggestions?  We uses
> Surgipath  EM400 for embedding paraffin and and their
> infiltration medium.  Is
> this to soft? The lowest we can turn our waterbath is 38
> degrees C.
> 
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 





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Re: [Histonet] problem eyes

[Rene J Buesa]

Add a few drops (4-5) of liquid detergent, but not dishwasher detergent 
(because it will dissolve the paraffin).
rené J.

--- On Wed, 3/4/09, Perry, Margaret  wrote:

From: Perry, Margaret 
Subject: [Histonet] problem eyes
To: "histonet@lists.utsouthwestern.edu" 
Date: Wednesday, March 4, 2009, 4:04 PM

Please help!  We have been trying to cut whole eyes from a pig.  They need to be
nice enough for a publication.  We are having the devil of a time because no
matter what we do there are wrinkles.  If we turn the waterbath up to stretch
things out the retina detaches.  Do any of you have suggestions?  We uses
Surgipath  EM400 for embedding paraffin and and their infiltration medium.  Is
this to soft? The lowest we can turn our waterbath is 38 degrees C.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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[Histonet] problem eyes

[Perry, Margaret]

Please help!  We have been trying to cut whole eyes from a pig.  They need to 
be nice enough for a publication.  We are having the devil of a time because no 
matter what we do there are wrinkles.  If we turn the waterbath up to stretch 
things out the retina detaches.  Do any of you have suggestions?  We uses 
Surgipath  EM400 for embedding paraffin and and their infiltration medium.  Is 
this to soft? The lowest we can turn our waterbath is 38 degrees C.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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[Histonet] (no subject)

[Matthew Bingham]

As many of you may or may not know, the Histobath countertop freezing bath has
been discontinued by Thermo (formerly known as Thermo-Shandon, Shandon-Lipshaw,
etc.). I was told that the freon that is used to allow the unit to cool to -60
is no longer allowed by the EPA to be used in ultra low refrigeration units. I
know there is a floor model, the Clini-RF, at brightinstruments.com which uses a
two types of freon in isolated side by side systems, but it is a rather large
unit (20"D x 18"W x 40"H). Our frozen section room, as are most, is a small
room with very limited space. Therefore, have any of you found a solution for a
countertop model (i.e. 19"D x 9"W x 15"H)freezing bath to be used in frozen
section rooms? I appreciate the help.

Thanks,

Matthew Bingham, PA(ASCP)CMAnatomic Pathology Supervisor
Phoenix Children's Hospital
Department of Pathology
1919 E. Thomas Road
Phoenix, AZ 85016
phone: 602-546-1318
fax: 602-546-1284
email: mbing...@phoenixchildrens.com



  
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[Histonet] vector blue- xylene soluble- any alternatives

[anjan kumar]

hello everyone,

i have a problem which i dont know how to tackle, the 
problem is that i have purchased vector blue as a chromogen and it is xylene 
soluble can anyone suggest me how to avoid this as funds are low i cant 
purchase xylene substitutes. 

 

second question been, ...sorry this has been a addition ..

what is the difference between the DPX mountant and other permanent mounting 
media like vectamount. kindly answer these questions...

 

regards,

Anjan Kumar

Junior research scientist

Veterinary Pathology

Madras veterinary college.

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[Histonet] staining for elastic fiber of artery in rat lung

[Amy Lee]

Hello histonetters,
 
Would you teach me what is best stain for elastic fiber in rat lung except 
weigert's stain?
 
I want to look at arteries.
 
Thanks in advance,
Weihua



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Re: [Histonet] Lymph Nodes on Colon Cancers

[Rene J Buesa]

If there are not 12 to be found, document what you did, how you did it, and how 
many you found.
You cannot find what is not there, but make sure you tried your best.
René J.

--- On Wed, 3/4/09, Smith Wanda  wrote:

From: Smith Wanda 
Subject: [Histonet] Lymph Nodes on Colon Cancers
To: "histonet@lists.utsouthwestern.edu" 
Date: Wednesday, March 4, 2009, 11:57 AM

Good Morning,
What is everyone doing to meet the recommendation from the American College of
Surgeons and American Cancer Society regarding finding a minimum of 12 lymph
nodes on colon cancer cases?  Especially when there are not 12 lymph nodes to be
found???
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax


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[Histonet] Lymph Nodes on Colon Cancers

[Smith Wanda]

Good Morning,
What is everyone doing to meet the recommendation from the American College of 
Surgeons and American Cancer Society regarding finding a minimum of 12 lymph 
nodes on colon cancer cases?  Especially when there are not 12 lymph nodes to 
be found???
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax


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[Histonet] RE: Histonet Digest, Vol 64, Issue 5 - CASSETTE HOLDERS

[Kay, Karen]

Good Morning Victoria

I believe Market Lab carries plastic cassette holders.

Karen J Kay, MLT
Pathology Supervisor
Chinook Health Laboratory
Chinook Regional Hospital
960 - 19 st South
Lethbridge, Alberta, Canada
T1J 1W5
(403) 388 - 6061 (Phone)
(403) 388 - 6067 (Fax)



Message: 6
Date: Tue, 3 Mar 2009 15:16:41 -0500
From: "Spoon, Victoria" 
Subject: [Histonet] Cassette holders
To: 
Message-ID: <415700fc732de14491a3e39367834f7702227...@ex3.bassett.org>
Content-Type: text/plain;   charset="us-ascii"

Does anyone know where to purchase cassette holders?  Plastic or metal
holders for cassettes - 5 across and 4- 5 deep.

Thanks,




-

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[Histonet] contrast

[Molinari, Betsy]

Hi,

 I have some mouse hindlimbs that have catheterized with Omnipaque. The
Omnipaque contains 302 ml of iohexol which is equivalent to 140 mg of
organic iodine per ml. The mice will be sacrificed immediately after
being radiographed. They would like them to be processed in paraffin. I
am wondering if this will have any effect on the muscle.

Thanks.

 

Betsy Molinari HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812

 

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[Histonet] histo equipment for sale

[nilfgaard]

Hello histonetters, 

I have the following, in perfect working condition, items for sale: 



tissue processor VIP1000 benchtop, just refurbished 

Shandon 24-3 slide stainer 

Surgipath PC3001, PC3002 embedding center 

ducktless hood with new carbon filters 

hood the a super quite vent system (for outside venting) 

mini VWR hybridization oven 

TBS warter bath - like new 



ONLY, if you are seriously interested, email me at sight...@comcast.net 

All pieces are located in Chicagoland. Pics available, can ship UPS ground. 



thanks 

Sightdog 
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Re: [Histonet] Cresyl violet problems

[TF]

Try some freshly prepared cresyl violet solutions?

2009-03-04 



TF 



发件人： Walters, Katherine S 
发送时间： 2009-03-04  04:09:07 
收件人： histo...@pathology.swmed.edu 
抄送： 
主题： [Histonet] Cresyl violet problems 
 
To the "brainy" histo-people,
I have a student doing a cresyl violet stain on some frozen (40um) brain
sections.  He has been doing this routinely for a couple of months, but
his last run looks very odd.  There appears to be an area on each
section where there is no staining.  At first glance I thought it was a
fixation problem, but the unstained portion of the tissue is in a
different area as you move from slice to slice (these are serial
sections),   He stains in a Copeland jar and assures me that his stain
is well homogenized.  Could this be some kind of moisture problem?
Thanks for your thoughts.
Katherine Walters
Histology Director
Central Microscopy Research Facilities
85 Eckstein Medical Research Building
University of Iowa
Iowa City, Iowa 52242-1101
katherine-walt...@uiowa.edu
www.uiowa.edu/~cemrf
Notice: This UI Health Care e-mail (including attachments) is covered by the 
Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and 
may be legally privileged.  If you are not the intended recipient, you are 
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have received the message in error, then delete it.  Thank you.
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[Histonet] Cholin acetyltransferase antibody

[Suhyoung Jeong]

Does anyone know a good anti-cholin acetyltransferase (ChAT) antibody for
mouse spinal cord to do immunohistochemistry (frozen section)? I see a
couple of vendors with more than a couple of clon names.

Moreover, somebody recommended this neurotransmitter as a motor neuronal
marker. If you can give me a better suggestion, it will be greatly
appreciated.

Thank you in advance!!

Sincerely, Suh
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[Histonet] distilled water...again

[kristen arvidson]

How is everyone getting the water?  We have a distiller in the lab but it will 
need to be replaced soon.  The question is another distiller or buy bottled??



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Re: [Histonet] distilled water...again

[Merced Leiker]

it may be worth your while to buy another distiller...bottled water will 
cost more over time...


--On Wednesday, March 04, 2009 6:37 AM -0800 kristen arvidson 
 wrote:



How is everyone getting the water?  We have a distiller in the lab but it
will need to be replaced soon.  The question is another distiller or buy
bottled??



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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Re: [Histonet] We are buying a new Tissue Processor and need imput

[Rene J Buesa]

I would by the VIP6 with total confidence.
René J.

--- On Wed, 3/4/09, Farnham, Lori  wrote:

From: Farnham, Lori 
Subject: [Histonet] We are buying a new Tissue Processor and need imput
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 4, 2009, 9:11 AM

Hi All, 
We are a small Pathology Lab which processes a little over 7000 surgical
cases a year. We have a mixed variety of specimens (GI, GYN, skins,
etc).
We have been approved to buy a new tissue processor and are looking at
the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these
processors would be greatly appreciated.
Thanks! 
Lori Ann Farnham, B.S.CT(ASCP)
Cytotechnologist
St. Mary's Hospital at Amsterdam
Phone 518-841-7296
e-mail farnh...@smha.org


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RE: [Histonet] Storage of muscles

[Hofecker, Jennifer L]

I agree with Tony that a -70 freezer is ideal. We wrap our samples in aluminum 
foil and then place in a plastic container. If you only have access to a -20 
freezer, it's helpful to place cold packs in the freezer, near where you will 
store the muscle samples. When a -20 freezer cycles, the temperature can 
fluctuate enough to create artifact. We've found that if we keep several 
disposable cold packs in a -20 that we can keep the temperature more 
consistent. 
Good Luck!

Jennifer L. Hofecker HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
Nashville, TN 
ph 615.343.0083
fax 615.343.7089

-Original Message-
From: Tony Henwood [mailto:antho...@chw.edu.au] 
Sent: Tuesday, March 03, 2009 3:59 PM
To: rennie1...@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Storage of muscles

Minus 70 degrees
A normal minus 20 freezer tends to dessicate the tissue

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Wednesday, 4 March 2009 7:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Storage of muscles


Hello,
We are having problems with artifact after our muscles have been stored for 
long periods of time. Can anyone tell me how they store their muscle specimens 
for long-term?
 
Thank you,
Adrienne Anderson
Duke University Health System
rennie1...@yahoo.com
 


  
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[Histonet] We are buying a new Tissue Processor and need imput

[Farnham, Lori]

Hi All, 
We are a small Pathology Lab which processes a little over 7000 surgical
cases a year. We have a mixed variety of specimens (GI, GYN, skins,
etc).
We have been approved to buy a new tissue processor and are looking at
the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these
processors would be greatly appreciated.
Thanks! 
Lori Ann Farnham, B.S.CT(ASCP)
Cytotechnologist
St. Mary's Hospital at Amsterdam
Phone 518-841-7296
e-mail farnh...@smha.org


CONFIDENTIALITY NOTICE: This email message and any accompanying data or files 
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named recipient(s). If you are not the intended recipient(s), you are hereby 
notified that the dissemination, distribution, and or copying of this message 
is strictly prohibited. If you receive this message in error, or are not the 
named recipient(s), please notify the sender at the email address above, delete 
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Re: [Histonet] immunohistochemistry with CD markers

[anh2006]

What markers and specific clones do you need help with specifically? Also mouse 
testis really needs a PFA fix. Even if you do fresh frozen sections I suggest a 
short (10 min) fix in PFA rather than acetone. 

-Original Message-
From: "Marti Gaudes, Merce" 

Date: Wed, 04 Mar 2009 09:51:39 
To: 
Subject: [Histonet] immunohistochemistry with CD markers


Hello!

 

Can anyone help us with CD markers? We want to use some CD markers on  mouse 
testis, and the antibodies data sheets explain that only freeze samples, 
cryosectioned and post-fixed with acetone can be used. We tried to do this 
protocols, but without any result. We frozen the sample with liquid nitrogen, 
then we sectioned the sample at the cryostat, and we fixed the sections with 
acetone over the slides. The morphology was very damaged, and we couldn't 
obtain any staining.

Does anyone explain us the details or tricks of the technique, to improve our 
results?

Thanks a lot!

 

 

Mercè Martí Gaudes

Cap de Servei

Histology and Bioimaging

Centre de Medicina Regenerativa de Barcelona (CMR[B])
Dr. Aiguader, 88  7ena planta
08003 Barcelona

mma...@cmrb.eu  

Tel: +34  93 316 03 51

Fax: +34 93 224 10 83

 




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[Histonet] immunohistochemistry with CD markers

[Marti Gaudes, Merce]

Hello!

 

Can anyone help us with CD markers? We want to use some CD markers on  mouse 
testis, and the antibodies data sheets explain that only freeze samples, 
cryosectioned and post-fixed with acetone can be used. We tried to do this 
protocols, but without any result. We frozen the sample with liquid nitrogen, 
then we sectioned the sample at the cryostat, and we fixed the sections with 
acetone over the slides. The morphology was very damaged, and we couldn't 
obtain any staining.

Does anyone explain us the details or tricks of the technique, to improve our 
results?

Thanks a lot!

 

 

Mercè Martí Gaudes

Cap de Servei

Histology and Bioimaging

Centre de Medicina Regenerativa de Barcelona (CMR[B])
Dr. Aiguader, 88  7ena planta
08003 Barcelona

mma...@cmrb.eu  

Tel: +34  93 316 03 51

Fax: +34 93 224 10 83

 




Abans d'imprimir aquest missatge, si us plau, comprova que és realment
necessari. El medi ambient és cosa de tots.

Antes de imprimir este mensaje, por favor, comprueba que es realmente
necesario. El medio ambiente es cosa de todos.

Before printing this e-mail, please make certain it is absolutely necessary.
The environment is everybody's business.


La informació continguda en aquest missatge i en qualsevol fitxer 
adjunt és confidencial, privada i d'ús exclusiu per al destinatari.
Si no és la persona a la qual anava dirigida aquesta informació, si us 
plau, notifiqui immediatament l'enviament erroni al remitent i esborri 
el missatge. Qualsevol còpia, divulgació, distribució o utilització no 
autoritzada d'aquest correu electrònic i dels seus adjunts està 
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La información contenida en este mensaje y en cualquier fichero 
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