Fwd: [Histonet] Tissue specimen disposal

[Mark J. Griffith]

Lisa,

We have a few customers who use the Vyleater to shred the specimen 
vial and reclaim the formalin for bulk disposal.


By gathering the formalin in bulk form - the waste disposal savings 
can be considerable.


Most are disposing of the shredded plastic as chemical hazardous or 
bio hazardous waste depending upon how their safety folks evaluate 
the wastestream. But in either case, removing the liquid makes a big 
difference.


Contact me directly if you would like to get more details.

Mark Griffith
S & G Enterprises, Inc.
N115 W19000 Edison Drive
Germantown, WI  53022   USA
Tel: 262/251-8300
US/Canada toll-free: 800/233-3721
Fax: 262/251-1616

Original Message

Date: Mon, 09 Mar 2009 15:51:15 -0400
From: "Lisa S. Black" 
To: 
Subject: [Histonet] Tissue specimen disposal
Sender: histonet-boun...@lists.utsouthwestern.edu

1.  How do you dispose of formalin fixed tissue specimens?
2.  If you use a vendor, do you pour off the formalin first and have 
two separate waste companies remove the fluid and tissue?

3.  If you use one vendor for both, please provide vendor name.

Thanks,
Lisa


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RE: [Histonet] Tissue specimen disposal

[Laurie Colbert]

We have an outside vendor, Stericycle, separate the formalin and the
tissue, and they take both for disposal.  I am in the Los Angeles area.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa S.
Black
Sent: Monday, March 09, 2009 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue specimen disposal

1.  How do you dispose of formalin fixed tissue specimens?  
2.  If you use a vendor, do you pour off the formalin first and have two
separate waste companies remove the fluid and tissue?  
3.  If you use one vendor for both, please provide vendor name.

Thanks,
Lisa


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RE: [Histonet] Tissue specimen disposal

[Sherwood, Margaret]

Lisa,

I just had a similar situation, and our waste management company just had me put
the tightly sealed containers in plastic bags and label with hazardous waste
label.  I could put multiple samples in a plastic bag.  I thought I would have
to separate the tissue from the fixative, but that was not the case.

I am in Boston, and we deal with Triumverate Environmental.

Hope this helps!

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa S. Black
Sent: Monday, March 09, 2009 3:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue specimen disposal

1.  How do you dispose of formalin fixed tissue specimens?  
2.  If you use a vendor, do you pour off the formalin first and have two
separate waste companies remove the fluid and tissue?  
3.  If you use one vendor for both, please provide vendor name.

Thanks,
Lisa


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[Histonet] Tissue specimen disposal

[Lisa S. Black]

1.  How do you dispose of formalin fixed tissue specimens?  
2.  If you use a vendor, do you pour off the formalin first and have two 
separate waste companies remove the fluid and tissue?  
3.  If you use one vendor for both, please provide vendor name.

Thanks,
Lisa


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[Histonet] Job Opportunity: Histotechnologist

[Crawford, Jennifer]

Title: Histotechnologist

 

Location: South suburbs of Chicago

 

Pay: $25-27/hr based on experience

 

Employment Type: 6 month contract to hire

 

Shift: 1st shift (4:30 AM - 2:30 PM or 5:30 AM - 3:30 PM, Monday through
Thursday with rotating Saturday schedule)

Part time positions are available as well!!!

 

Requirements: Under the leadership of the Director and section
supervisor, the Histology Technologist receives and properly processes
specimens for the Pathologists in a timely manner, reviews test results,
and participates in Quality Assurance. The histology technician will
also maintain slide files and records, assist the Pathologist and
function in a limited capacity as a cytotechnologist as needed. 

 

Specific required skills: Immunostaining (Ventana Stainer), Cytology,
ASCP certification is a must.

 

***Please contact Jen Crawford at jcraw...@aerotek.com or by phone at
(847) 221-1358. ***

 

Jen Crawford
Scientific Recruiter
Aerotek Scientific Staffing
Phone: 847.221.1358
Fax: 847.303.2370 
www.aerotek.com   
Please do not keep me a secret...a referral is the best compliment that
I can receive! 

Aerotek has become the #1 staffing provider within the sciences!

http://www.aerotek.com/About-Us/Press-Release-10147.news
 

 




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[Histonet] avidin/biotin blocking???

[Eva Permaul]

Good afternoon out there in histoland,
I am trying to find a cheaper alternative to the avidin/biotin blocking 
system we are using now so that we can use it for larger bulk staining 
cases. Right now we only use it for small hand staining cases.
I read somewhere that there are those who make these reagents 
themselves. Would anyone be willing to share their recipes and how do 
they compare to company bought ones?

Thanks for your help,
Eva Permaul
Georgetown University

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[Histonet] RE: Histonet Digest, Vol 64, Issue 15

[Kay, Karen]

Hello Andrea,
We purchase our Mollifex from VWR Scientific

Karen J Kay, MLT
Pathology Supervisor
Chinook Health Laboratory,Chinook Regional Hospital
960 - 19 st South
Lethbridge, Alberta,CANADA
T1J 1W5
(403) 388 - 6061 (Phone)
(403) 388 - 6067 (Fax)

  

--

Message: 14
Date: Mon, 09 Mar 2009 08:50:13 -0700
From: Andrea Grantham 
Subject: [Histonet] mollifax
To: histonet@lists.utsouthwestern.edu
Message-ID:

<6.2.3.4.1.20090309084808.0272d...@algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Good Morning,
Just wondering - Who sells Mollifax? I'm looking for a good way to 
soften the exoskeleton on insects of the larger variety.

Andi
.
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist   University of Arizona   :
: (office:  AHSC 4212)  P.O. Box 245044 :
: (voice:  520-626-4415)Tucson, AZ  85724-5044USA   :
: (FAX:  520-626-2097)  (email:  algra...@u.arizona.edu)   :
:...:
   http://www.cba.arizona.edu/histology-lab.html





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Re: [Histonet] mollifax

[Andrea Grantham]

Excuse me - if I could spell then google might have worked better! 
Got it! Thanks!


Andi




At 08:50 AM 3/9/2009, Andrea Grantham wrote:

Good Morning,
Just wondering - Who sells Mollifax? I'm looking for a good way to 
soften the exoskeleton on insects of the larger variety.


Andi
.
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist   University of Arizona   :
: (office:  AHSC 4212)  P.O. Box 245044 :
: (voice:  520-626-4415)Tucson, AZ  85724-5044USA   :
: (FAX:  520-626-2097)  (email:  algra...@u.arizona.edu)   :
:...:
  http://www.cba.arizona.edu/histology-lab.html


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.
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist   University of Arizona   :
: (office:  AHSC 4212)  P.O. Box 245044 :
: (voice:  520-626-4415)Tucson, AZ  85724-5044USA   :
: (FAX:  520-626-2097)  (email:  algra...@u.arizona.edu)   :
:...:
  http://www.cba.arizona.edu/histology-lab.html


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Re: [Histonet] new FS stain problem

[Lynette Pavelich]

I have found that the longer I delay getting the frozen section into fixative, 
the worse the staining/results.  

Hope this helps,
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503

ph:  810-257-9948
fax:  810-762-7082
>>> Rene J Buesa  03/09/09 11:41 AM >>>
I am not completely sure but perhaps that first section taken after cutting 
down into the block is affected by that triming. Why don't you just discard 
that first section and start using the following 3 to see if they all stain 
corretly?
René J.

--- On Mon, 3/9/09, King, Laurie  wrote:

From: King, Laurie 
Subject: [Histonet] new FS stain problem
To: "histonet@lists.utsouthwestern.edu" 
Date: Monday, March 9, 2009, 11:05 AM

Good day all,

I am doing frozen sectioning at three different facilities in two towns, and
this problem is identical at each. 
The first section I keep, I'm in the habit of placing at the frosted end of
the slide. No matter if I take one, two, three or more, that first section is
almost non existent hematoxylin, very blurry staining. 

Thinking I was having contamination, solution level problems, I checked all
levels and made sure to have no dripping from the top of the slide, still the
same problem. Then, on a whim, I put the first section at the bottom of the
slide, and two more working my way toward the frosted end. The first section,
placed at the bottom of the slide now, still had  the poor staining. Any ideas?

I use OCT
fix in half and half formalin/95% ETOH
DW
Harris
tap
bluing
tap
eosin
95% X2
100% X2
xylene X2

Let's just say I am a "seasoned" tech, been doing this for almost
30 years, so I'm not a little embarrassed by my inability to figure this one
out.

Laurie King
Marshfield Clinic
Eau Claire/Rice Lake Centers
Eau Claire WI





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RE: [Histonet] new FS stain problem

[Bonner, Janet]

When you put the first frozen section on the slide near the label, dip it label 
side down in the FS fixative so that the fixative just covers the section.  Put 
the consecutive section on the non-label end and then put the whole slide in 
fixative..Have to fix those sections ASAP!!
 
Janet L. Bonner, HTL (ASCP)
Pathology Laboratory
Florida Hospital Winter Park



From: histonet-boun...@lists.utsouthwestern.edu on behalf of King, Laurie
Sent: Mon 3/9/2009 11:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] new FS stain problem



Good day all,

I am doing frozen sectioning at three different facilities in two towns, and 
this problem is identical at each.
The first section I keep, I'm in the habit of placing at the frosted end of the 
slide. No matter if I take one, two, three or more, that first section is 
almost non existent hematoxylin, very blurry staining.

Thinking I was having contamination, solution level problems, I checked all 
levels and made sure to have no dripping from the top of the slide, still the 
same problem. Then, on a whim, I put the first section at the bottom of the 
slide, and two more working my way toward the frosted end. The first section, 
placed at the bottom of the slide now, still had  the poor staining. Any ideas?

I use OCT
fix in half and half formalin/95% ETOH
DW
Harris
tap
bluing
tap
eosin
95% X2
100% X2
xylene X2

Let's just say I am a "seasoned" tech, been doing this for almost 30 years, so 
I'm not a little embarrassed by my inability to figure this one out.

Laurie King
Marshfield Clinic
Eau Claire/Rice Lake Centers
Eau Claire WI








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[Histonet] mollifax

[Andrea Grantham]

Good Morning,
Just wondering - Who sells Mollifax? I'm looking for a good way to 
soften the exoskeleton on insects of the larger variety.


Andi
.
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist   University of Arizona   :
: (office:  AHSC 4212)  P.O. Box 245044 :
: (voice:  520-626-4415)Tucson, AZ  85724-5044USA   :
: (FAX:  520-626-2097)  (email:  algra...@u.arizona.edu)   :
:...:
  http://www.cba.arizona.edu/histology-lab.html


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Re: [Histonet] Bone marrow aspirates from mouse

[Rene J Buesa]

Use the same thing you would use for a blood smear: methanol 5 minutes and air 
dry it.
René J.

--- On Mon, 3/9/09, Vanessa J. Phelan  wrote:

From: Vanessa J. Phelan 
Subject: [Histonet] Bone marrow aspirates from mouse
To: histonet@lists.utsouthwestern.edu
Date: Monday, March 9, 2009, 11:39 AM

Hi everyone,

I was wondering if anyone had any experience with taking bone marrow
aspirates from mice for analysis, staining with Giemsa and for IHC?
My query is, once the aspirate has been taken and then cytospinned down and
spread on the slide, what fixative would you use at this point before
staining? Maybe formal alcohol? PFA? Or maybe even cytofix spray? also where
to store the slides? -80 freezer?

Any help on this would be much appreciated, as this procedure has not been
carried out here before and I have no experience in this either.

Thanks a mill, 

Vanessa


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Re: [Histonet] new FS stain problem

[Rene J Buesa]

I am not completely sure but perhaps that first section taken after cutting 
down into the block is affected by that triming. Why don't you just discard 
that first section and start using the following 3 to see if they all stain 
corretly?
René J.

--- On Mon, 3/9/09, King, Laurie  wrote:

From: King, Laurie 
Subject: [Histonet] new FS stain problem
To: "histonet@lists.utsouthwestern.edu" 
Date: Monday, March 9, 2009, 11:05 AM

Good day all,

I am doing frozen sectioning at three different facilities in two towns, and
this problem is identical at each. 
The first section I keep, I'm in the habit of placing at the frosted end of
the slide. No matter if I take one, two, three or more, that first section is
almost non existent hematoxylin, very blurry staining. 

Thinking I was having contamination, solution level problems, I checked all
levels and made sure to have no dripping from the top of the slide, still the
same problem. Then, on a whim, I put the first section at the bottom of the
slide, and two more working my way toward the frosted end. The first section,
placed at the bottom of the slide now, still had  the poor staining. Any ideas?

I use OCT
fix in half and half formalin/95% ETOH
DW
Harris
tap
bluing
tap
eosin
95% X2
100% X2
xylene X2

Let's just say I am a "seasoned" tech, been doing this for almost
30 years, so I'm not a little embarrassed by my inability to figure this one
out.

Laurie King
Marshfield Clinic
Eau Claire/Rice Lake Centers
Eau Claire WI





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[Histonet] Bone marrow aspirates from mouse

[Vanessa J. Phelan]

Hi everyone,

I was wondering if anyone had any experience with taking bone marrow
aspirates from mice for analysis, staining with Giemsa and for IHC?
My query is, once the aspirate has been taken and then cytospinned down and
spread on the slide, what fixative would you use at this point before
staining? Maybe formal alcohol? PFA? Or maybe even cytofix spray? also where
to store the slides? -80 freezer?

Any help on this would be much appreciated, as this procedure has not been
carried out here before and I have no experience in this either.

Thanks a mill, 

Vanessa


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[Histonet] Zinc Formalin

[Kang, Joseph J]

Hey Histonetters,

Can anyone tell me about your experiences doing bone IHC using zinc
formalin fixative?

I also would like to know if anyone have processed rat paws and kee
joints using this fixative. If you could provide the optimal fixation
time for these tissues would be greatly appreciated.

Thanks.

Joe

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Re: [Histonet] Competency checklist

[Amy Porter]

The Michigan Society for Histotechnology has a handbook available for 
purchase through their website www.mihisto.org



Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  port...@msu.edu
Web:  www.humanpathology.msu.edu
- Original Message - 
From: "Vacca Jessica" 

To: 
Sent: Monday, March 09, 2009 10:53 AM
Subject: [Histonet] Competency checklist


Does anyone have a competency checklist they would like to share, I know all 
labs are different but if you'd like to share yours, please email it to 
jessica.va...@hcahealthcare.com This would be for Technologist.

Thanks
Jessica Vacca
Histology Supervisor
119 Oakfield Dr
Brandon Fl 33511
(813) 571-5193
(813) 571-5169 FAX




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[Histonet] ot? rap for hox genes

[Emily Sours]

http://tierneylab.blogs.nytimes.com/2009/03/09/rappin-for-science/?hp

i love this song--i work with hox genes in chick

emily
-- 
prometheus, thief of light, giver of light, bound by the gods, must have
been a book.
-mark danielewski, house of leaves
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[Histonet] new FS stain problem

[King, Laurie]

Good day all,

I am doing frozen sectioning at three different facilities in two towns, and 
this problem is identical at each. 
The first section I keep, I'm in the habit of placing at the frosted end of the 
slide. No matter if I take one, two, three or more, that first section is 
almost non existent hematoxylin, very blurry staining. 

Thinking I was having contamination, solution level problems, I checked all 
levels and made sure to have no dripping from the top of the slide, still the 
same problem. Then, on a whim, I put the first section at the bottom of the 
slide, and two more working my way toward the frosted end. The first section, 
placed at the bottom of the slide now, still had  the poor staining. Any ideas?

I use OCT
fix in half and half formalin/95% ETOH
DW
Harris
tap
bluing
tap
eosin
95% X2
100% X2
xylene X2

Let's just say I am a "seasoned" tech, been doing this for almost 30 years, so 
I'm not a little embarrassed by my inability to figure this one out.

Laurie King
Marshfield Clinic
Eau Claire/Rice Lake Centers
Eau Claire WI





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Re: [Histonet] Cassettes and Processing and Fixation ~ Oh My!

[Sean McBride]

Laura,

1.  Specimen size dictates to cassette size for our lab.
2.  Yes, we use sectioned racks & organize our specimens in sequential order
in case the marker is accidentally solvated during the processing.
3.  Again, specimen size and tissue type dictate to our fixation schedule,
but we never formalin fixate a specimen for only 5 minutes.  I prefer to
error on the side of caution.

~Sean McBride


Senior Researcher
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-901-7540 (m)
412-268-8641 (fax)
smcbr...@cs.cmu.edu


On 3/9/09 10:43 AM, "Jones, Laura"  wrote:

> Hi all.  We are having a discussion here about everything in my subject line,
> but to be more specific:
> 
> 1.  Do you all use different types of cassettes for different sizes of
> tissues?  We have screen cassettes for prostate biopsies, and biopsy cassettes
> for skins, and regular flow through type for larger-than-they-should-be pieces
> that have grid marks on them.
> 
> 2.  When you load your cassettes on the processor, do you use the "organized"
> basket that spaces them out or the "random" basket?  If you use the "random"
> method, how tightly do you feel the cassettes can be packed?  Isn't it true
> that if they are packed too tightly, the fixation of the tissue will be
> compromised?  And, how does everyone use agitation/stirring on the processor?
> We have always used it, but are wondering how others are doing things.
> 
> 3.  Finally, for a run of combination tissues as described above, what would
> be your recommended time in formalins?  We know all Pathologists are in a
> hurry for slides, but is a 5 minute station ever acceptable?
> 
> This is all a result of weeks of discussion about possible changes we could
> make here, and varying things we have learned over the years... we'd just like
> to hear what everyone else is doing.  For instance: would it be simpler to use
> biopsy cassettes for everything?  Should we consider using the random basket
> instead of the organized one?  How far back could we cut our processing times
> to expedite things?  We'd really appreciate the input of all you experts.
> Thanks in advance!
> 
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 








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Re: [Histonet] Cassettes and Processing and Fixation ~ Oh My!

[Rene J Buesa]

Laura:
Trying to sum up your concerns, this is what I have always done:
1- just one type of cassette, with different colors for  rushes, regular, 
autopsies, bone marrows. If there were small biopsies they were placed between 
sponges. Bone marrow aspirates wrapped.
2- I always used the organized basket that allowed knowing the number and 
assured equal spacing between the cassettes
3- the fixation essentially took place before going into the tissue processor 
but 5 minutes in any event is just a joke.
4- the processing time will depend on the type of tissue and there are limits 
if you want not to waste the whole run because of inadequate processing
René J. 

--- On Mon, 3/9/09, Jones, Laura  wrote:

From: Jones, Laura 
Subject: [Histonet] Cassettes and Processing and Fixation ~ Oh My!
To: "Histonet (E-mail)" 
Date: Monday, March 9, 2009, 10:43 AM

Hi all.  We are having a discussion here about everything in my subject line,
but to be more specific:

1.  Do you all use different types of cassettes for different sizes of tissues?
 We have screen cassettes for prostate biopsies, and biopsy cassettes for skins,
and regular flow through type for larger-than-they-should-be pieces that have
grid marks on them.

2.  When you load your cassettes on the processor, do you use the
"organized" basket that spaces them out or the "random"
basket?  If you use the "random" method, how tightly do you feel the
cassettes can be packed?  Isn't it true that if they are packed too tightly,
the fixation of the tissue will be compromised?  And, how does everyone use
agitation/stirring on the processor?  We have always used it, but are wondering
how others are doing things.

3.  Finally, for a run of combination tissues as described above, what would be
your recommended time in formalins?  We know all Pathologists are in a hurry for
slides, but is a 5 minute station ever acceptable?

This is all a result of weeks of discussion about possible changes we could
make here, and varying things we have learned over the years... we'd just
like to hear what everyone else is doing.  For instance: would it be simpler to
use biopsy cassettes for everything?  Should we consider using the random basket
instead of the organized one?  How far back could we cut our processing times to
expedite things?  We'd really appreciate the input of all you experts. 
Thanks in advance!

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[Histonet] Competency checklist

[Vacca Jessica]

Does anyone have a competency checklist they would like to share, I know all 
labs are different but if you'd like to share yours, please email it to 
jessica.va...@hcahealthcare.com This would be for Technologist.
Thanks
Jessica Vacca
Histology Supervisor
119 Oakfield Dr
Brandon Fl 33511
(813) 571-5193
(813) 571-5169 FAX
  



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[Histonet] Cassettes and Processing and Fixation ~ Oh My!

[Jones, Laura]

Hi all.  We are having a discussion here about everything in my subject line, 
but to be more specific:

1.  Do you all use different types of cassettes for different sizes of tissues? 
 We have screen cassettes for prostate biopsies, and biopsy cassettes for 
skins, and regular flow through type for larger-than-they-should-be pieces that 
have grid marks on them.

2.  When you load your cassettes on the processor, do you use the "organized" 
basket that spaces them out or the "random" basket?  If you use the "random" 
method, how tightly do you feel the cassettes can be packed?  Isn't it true 
that if they are packed too tightly, the fixation of the tissue will be 
compromised?  And, how does everyone use agitation/stirring on the processor?  
We have always used it, but are wondering how others are doing things.

3.  Finally, for a run of combination tissues as described above, what would be 
your recommended time in formalins?  We know all Pathologists are in a hurry 
for slides, but is a 5 minute station ever acceptable?

This is all a result of weeks of discussion about possible changes we could 
make here, and varying things we have learned over the years... we'd just like 
to hear what everyone else is doing.  For instance: would it be simpler to use 
biopsy cassettes for everything?  Should we consider using the random basket 
instead of the organized one?  How far back could we cut our processing times 
to expedite things?  We'd really appreciate the input of all you experts.  
Thanks in advance!

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[Histonet] IHC for adenoviral vectors

[Kim Merriam]

Hi All,

I was wondering if anyone has ever done IHC to look for the presence of 
adenoviral vectors in tissue (ie - to track where the viral vector has spread 
in the body).

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 



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[Histonet] lab week acitivites

[Marshall, Kimberly]

Last year Histology put together lab week, some of the ideas we came up
with

 

Make up a song for the Dept, and had other areas judge (Silly but we all
had fun make in up the riffs) sending a example

Well you can tell by the way I cut my block

I'm a Histotech, no cuttin' through the block

Block's cold and slides are warm

I've been a cuttin' storm, Since early morn

Processed all night, cut today

And you may stain another way

We can try, to understand

The NSH effect on man

 

We also made t-shrits or decorated lab coats with tools in the different
depts..

 

Just some ideas. Hope it helps

 

 


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