RE: [Histonet] QIHC certificate

[Lee & Peggy Wenk]

>From the ASCP Board of Registry webpage:
www.ascp.org/bor
Click on Qualifications
Click on IHC

There are 3 routes. To summarize, all experience must be within the last 5
years, in a US lab, Canadian lab, or CAP/JC accredited lab:
1. ASCP certified technologist + 6 months full time IHC experience
2. ASCP certified technian + 12 months full time IHC experience
3. BA/BS degree or higher from a regionally accredited college/university +
18 months full time IHC experience

Full time acceptable experience must include:
- Immunohistochemical and/or Immunofluorescence Preparations
   All of the following should have been performed by the applicant: 
   - Staining technique 
   - Selection of proper control material 
   - Titration of immunologic reagents 
- Quality Assurance
   The applicant should have participated in Quality Assurance related to
all of the following: 
   - Specimen fixation, processing, microtomy 
   - Reagent selection, preparation, storage, disposal 
   - Method selection, validation, documentation 
   - Quality control 
   - Safety 

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO
Sent: Tuesday, March 24, 2009 4:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] QIHC certificate

Hi, all

  I have a question about how to QIHC certificate. We have several bilogical
scientists work as full-time employee doing IHC staining. But none of them
has HT certificate. In this case, can they get QIHC certificate? What kind
of courses need they to take?

  Thank you,

Ann Fu  Lab Manager
Dept. of Pathology
University of Flodrida
Gainesville, FL 32610


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RE: [Histonet] FW: formalin neutralizers

[Tony Henwood]

This is what we use:
Neutralization and Disposal of Formalin Fixative

10% Formalin can be neutralised with sodium bisulfite or concentrated ammonia. 
The reaction with ammonia results in the formation of hexamethylenetetramine 
(commonly known as hexamine or methenamine). This can then be safely disposed 
of either as a liquid fertiliser or via the sewage system (check with your 
local authority).

The reaction proceeds as follows: 

6 CH2O + 4 NH3 → C6H12N4 (Hexamethylenetetramine) + 6 H2O

Procedure:

1.  Before beginning, personnel must have the following safety equipment 
readily available in the event of an accidental spill:  sorbent material (spill 
pillows, bulk sorbent) formaldehyde rated respirator. 
2.  Personnel must wear a lab coat or apron, safety goggles and neoprene 
gloves. 
3.  A pH meter or pH paper
4.  To 1000 ml of 10% formalin (= 4% formaldehyde) add 56 ml of strong 
ammonia solution (27%). This will generate 31 g of hexamine (approximately a 3% 
solution).
5.  Stir well.  Reaction may produce heat.  
6.  Initially, the pH of the formaldehyde solution will be about 6.  As 
ammonia is added and stirred, a fluffy white precipitate will result.  Addition 
of sufficient ammonia will raise the pH to about 8.  Because the neutralization 
of the formaldehyde requires less molecules of ammonia than the apparent 
acid-base reaction supplies hydronium ions, the pH change from acid to base is 
used as an indicator that an excess of ammonia has been added. 
7.  Let set overnight (12 hours).
8.  The smell of formalin is greatly reduced or replaced by a faint whiff 
of ammonia.
9.  Schiff's reagent is perhaps the best, most sensitive and available 
reagent in any lab to test for the presence of aldehydes. If the "neutralized" 
formalin turns purplish with the addition of Schiff's reagent, it is not 
totally neutralized and you will need to add more ammonia.
10. Dispose of appropriately


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn
Sent: Wednesday, 25 March 2009 6:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: formalin neutralizers


 
 
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401



From: Burton, Lynn
Sent: Tue 3/24/2009 10:31 AM
To: histonet-bou...@lists.utsouthwestern.edu
Subject: formalin neutralizers


What is everyone using to neutral their formalin? Who has the best product for 
the price?
 
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401 ___
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[Histonet] IHC Stainer: Microm HMS 710i

[Katelin Lester]

I am looking for help/advice for using our automated immunostainer the
Microm HMS 710i  .(Our user manual says Richard Allan Sci and the labels on
the side say LabVision, we have calls into Thermo's LabVision department but
they are unsure if they can assist us)

Specifically, one of our problems is that some of our stains are producing
dark coloring around the edges of the tissue. I am having issues
troubleshooting and absolutely can not change any of the programming.

I have been using this stainer independently for over a year, taught to use
it by someone else who was taught by someone else. I am looking to change
some programming as well as troubleshoot. Anyone who has experience with
this stainer is welcome to contact me. 

Thank you,

Katelin Lester

Cutting Edge Histology Services, LLC

(503) 443-2157

 

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RE: [Histonet] LabVision line of instrumentation, antibodies and reagents

[Norton, Sally]

Hello,

We do IF's on renal biopsies and skin.  We were getting our antibodies
and Protein Block from ThermoShandon which is now Lab vision.  We are
now not getting our supplies when needed.  We have Protein Block on
order and were told we would not get any until end of April, which was
then amended to the end of March.  In the meantime we've run out and
can't do a renal biopsy until we get some.  Does anyone out there do IF
on Renal's and who is your supplier.  We are looking to find someone
new.

Thank you,

Sally Norton, HT
Seattle children's Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
rg...@bmnet.com
Sent: Tuesday, March 24, 2009 12:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] LabVision line of instrumentation,antibodies and
reagents

I was wondering if there are any clinical labs that use the LabVision
line
of instrumentation, antibodies and reagents.
We are a small hospital that does about 30 predilute antibodies and are
considering changing from our current vendor/instrument.

I would appreciate good, & bad experiences.  I don't want to go into any
change without knowing.

Thanks,

Renee Grow, BA., HT (ASCP)
rg...@bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax


This communication may contain protected health information (PHI) that
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note
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[Histonet] Looking for a CD20 that works on mouse tissue

[Colleen Forster]

Hello Histonetters,

Have a new project that wants to stain CD20 in mouse samples. One is 
brain and the other heart. Has anyone done this CD marker in mouse? if 
so, would you be willing to share with us!


Thanks in advance,

Colleen Forster
U of MN

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Re: [Histonet] questions re: fixing in general and Histochoice in particular

[Merced Leiker]

1. Generally 24 hrs for 2-3 mm thick piece. At least a 10:1 formalin:tissue 
volume.


2. Room temp or 4oC - I've heard debates, and I've heard it doesn't matter, 
but we do it at 4oC.



--On Tuesday, March 24, 2009 4:54 PM -0400 Jacqui Detmar 
 wrote:



Hi all.  Having a bit of an internal debate here, so I would like to get
the opinions of some of you in Histoland, please.  Here are the
questions:



1.When fixing with 10% NBF, for how long should you fix and what
volume ratio of fixative:tissue should you use?

2.   At what temperature should one be fixing tissues?



Regarding Histochoice:



1.For how long should you fix the tissue?

2.   What volume ratio of fixative:tissue should you use?

3.   How long can you store Histochoice-fixed tissues in 70%
ethanol?



I think that's about it.  Thanks in advance,



Jacqui



Jacqui Detmar, Post-doctoral Fellow

Samuel Lunenfeld Research Institute

Mount Sinai Hospital

25 Orde Street, room 6-1001AJ

Toronto, ON   M5T 3H7



Email:det...@lunenfeld.ca

Phone: 416-586-4800 x5607

Fax:416-586-5993

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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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[Histonet] questions re: fixing in general and Histochoice in particular

[Jacqui Detmar]

Hi all.  Having a bit of an internal debate here, so I would like to get
the opinions of some of you in Histoland, please.  Here are the
questions:  

 

1.When fixing with 10% NBF, for how long should you fix and what
volume ratio of fixative:tissue should you use?  

2.   At what temperature should one be fixing tissues?

 

Regarding Histochoice:

 

1.For how long should you fix the tissue?

2.   What volume ratio of fixative:tissue should you use?

3.   How long can you store Histochoice-fixed tissues in 70%
ethanol?

 

I think that's about it.  Thanks in advance,

 

Jacqui

 

Jacqui Detmar, Post-doctoral Fellow

Samuel Lunenfeld Research Institute

Mount Sinai Hospital

25 Orde Street, room 6-1001AJ

Toronto, ON   M5T 3H7

 

Email:det...@lunenfeld.ca

Phone: 416-586-4800 x5607

Fax:416-586-5993

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[Histonet] QIHC certificate

[FU,DONGTAO]

Hi, all

 I have a question about how to QIHC certificate. We have several 
bilogical scientists work as full-time employee doing IHC 
staining. But none of them has HT certificate. In this case, can 
they get QIHC certificate? What kind of courses need they to 
take?


 Thank you,

Ann Fu  Lab Manager
Dept. of Pathology
University of Flodrida
Gainesville, FL 32610


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Re: [Histonet] hematoxylin Question

[Jennifer MacDonald]

We use ours for a few weeks, but we do filter it before use.




kristen arvidson  
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/24/2009 07:48 AM
Please respond to
arvidsonkris...@yahoo.com


To
histonet 
cc

Subject
[Histonet] hematoxylin Question






Hello All,
How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is 
poured into a staining container?  The stain gets used so infrequently I 
would like to keep it as long as I can.  We currently dump it weekly 
regardless of use.  Thank you.
 
-Kristen


 
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[Histonet] California Symposium

[Jennifer MacDonald]

The final program is available for the California Society for 
Histotechnology annual symposium.  The symposium is scheduled for May 
14-17 in Millbrae.
If you are interested I can send you a word document or a pdf file.  It 
will be posted on the CSH website in the next day or so. 
http://www.californiahistology.org/
Jennifer

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Re: [Histonet] IF

[Rene J Buesa]

30 min at room temperature
René J.

--- On Tue, 3/24/09, Vanessa J. Phelan  wrote:

From: Vanessa J. Phelan 
Subject: [Histonet] IF
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, March 24, 2009, 12:17 PM

Hi everyone,

When carrying out IF on frozen sections how long after the cutting the sections
should you leave the slides to dry and where before starting the staining
procedure?

Thanks a mill,

V

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[Histonet] Re: CD31 anti-mouse on paraffin sections

[Lizbeth_Kelly]

Hi,

Has someone tried an antibody that would detect CD31 on mouse tissues 
using 10% NBF and not Zinc formalin?
Thanks,

Lizbeth Kelly, HT(ASCP), Q-IHC
Histology Department
Human Genome Sciences, Inc.
14200 Shady Grove Road
Rockville, MD  20850
240-314-4400 X2325
Fax: 301-354-4176
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RE: [Histonet] LabVision line of instrumentation, antibodies and reagents

[Linke, Noelle]

Hi Rene,

I love LabVision products!  Hopefully they won't be ruined by Fisher(I 
would put in names of all acquisitions but it would take too long to type)...

Noelle (LabVision cheerleader)


Noёlle Linke M.S., HTL(ASCP)QIHC
Manager, Histology Services
Department of Pathology & Laboratory Medicine
David Geffen School of Medicine at UCLA
Phone: 310-825-7397
Pager: 97471
nli...@mednet.ucla.edu



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rg...@bmnet.com
Sent: Tuesday, March 24, 2009 12:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] LabVision line of instrumentation, antibodies and reagents

I was wondering if there are any clinical labs that use the LabVision line
of instrumentation, antibodies and reagents.
We are a small hospital that does about 30 predilute antibodies and are
considering changing from our current vendor/instrument.

I would appreciate good, & bad experiences.  I don't want to go into any
change without knowing.

Thanks,

Renee Grow, BA., HT (ASCP)
rg...@bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax


This communication may contain protected health information (PHI) that is
legally protected from inappropriate disclosure by the Privacy Standards of
the Health Insurance Portability and Accountability Act (HIPAA) and
relevant Tennessee Laws. If you are not the intended recipient, please note
that any  dissemination, distribution or copying of this communication is
strictly prohibited. If you have received this message in error, you should
notify the sender immediately by telephone or by return e-mail and delete
this message from your computer. Direct questions to the Blount Memorial
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[Histonet] FW: formalin neutralizers

[Burton, Lynn]

 
 
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401



From: Burton, Lynn
Sent: Tue 3/24/2009 10:31 AM
To: histonet-bou...@lists.utsouthwestern.edu
Subject: formalin neutralizers


What is everyone using to neutral their formalin? Who has the best product for 
the price?
 
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
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[Histonet] re:microtome questions

[Matthew Close]

I'd go for the old AO 820.  They can be used by any and everyone and its
nearly impossible to break them.
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[Histonet] RE: Histonet Digest, Vol 64, Issue 42 JCAHO - Universal Protocol (Amy Self)

[Lecorchick, William]

We do 3 to 4 FNA's on a daily basis and no matter the site thyroid, liver ..Etc 
we go through the time out procedure every time, yes it may a little bit 
overkill for a palpable mass or using ultrasound guidance but none the less you 
must do it just like labeling the lido cane when it is the only syringe on the 
tray.
-Original Message-


--

Message: 3
Date: Mon, 23 Mar 2009 14:10:35 -0400
From: "Amy Self" 
Subject: [Histonet] JCAHO - Universal Protocol
To: 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Dear Histonetters,

Is there anyone out there that follows the JCAHO - Universal
Protocol/Time Out procedure when the pathologist perform his/her own
FNA's?  We have a pathologist that will perform FNA's when asked and I
was told that when he performs these procedures that we need to be
following this universal protocol/time out procedure that is followed in
the OR for surgeries.  I have all the info. from the OR but to me the
procedure that they follow is just too in-depth for the palpable masses
that are aspirated for cytology.  So I am turning to you all for help.
I was just wondering what other facilities were doing about this.  Any
help would be appreciated.  Thanks in advance.



Amy Self

Georgetown Hospital System

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[Histonet] LabVision line of instrumentation, antibodies and reagents

[rgrow]

I was wondering if there are any clinical labs that use the LabVision line
of instrumentation, antibodies and reagents.
We are a small hospital that does about 30 predilute antibodies and are
considering changing from our current vendor/instrument.

I would appreciate good, & bad experiences.  I don't want to go into any
change without knowing.

Thanks,

Renee Grow, BA., HT (ASCP)
rg...@bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax


This communication may contain protected health information (PHI) that is
legally protected from inappropriate disclosure by the Privacy Standards of
the Health Insurance Portability and Accountability Act (HIPAA) and
relevant Tennessee Laws. If you are not the intended recipient, please note
that any  dissemination, distribution or copying of this communication is
strictly prohibited. If you have received this message in error, you should
notify the sender immediately by telephone or by return e-mail and delete
this message from your computer. Direct questions to the Blount Memorial
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RE: [Histonet] re: water

[Rathborne, Toni]

Does anyone know of a method to change the pH of tap water without going 
through a lot of expense? Possibly a filter to put directly on the water line.
Thanks,
Toni

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Matthew
Close
Sent: Monday, March 23, 2009 12:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re: water


I think that distilled water starts out at a ph of around 6-6.5, but usually
DI water is closer to 7.  Over time, both will drop in pH unless you buy
specialized housing for the DI water.  Since many people doing IHC at my
university are using RO water, which has a pH similar to what the pH of the
tap source, I would say you are probably okay going with the distilled
water.  My word of caution is to check the level of air in your housing tank
and never let it drop below half.  Typically, the more air in the tank, the
more CO2 going into the water which will gradually lower the pH down to
5.1-5.5 territory.  Also, if you have the option to chose between using a
glass carboy and a plastic one to store water, always go with the glass.
While all of this has never been a real problem for me in terms of
analyzable results, I often wonder if it has anything to do with why,
sometimes, identical staining procedures on identially fixed tissues will
stain notably different.

Matt Close
Department of Biological Sciences
Lehigh University
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RE: [Histonet] Reference or service lab that does PDGFR-a staining?

[Jaime McMillan]

USLabs performs this test.

Jaime

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
Tim
Sent: Tuesday, March 24, 2009 10:39 AM
To: histonet
Subject: [Histonet] Reference or service lab that does PDGFR-a staining?

Hi, does anyone know of a reference or service lab that does immuno
staining for PDGFR-a,b (platelet derived growth factor receptor alpha
and/or beta.


Please note my new street address and phone number

Tim Morken
Technical Support Manager
Immunohistochemistry
Anatomical Pathology
Thermo Scientific
46360 Fremont Blvd
Fremont, CA 94538, USA

Ph: (510) 979-5000, ext 31819
or (800) 232-3342 ext 31819
Fax: (510) 979-5328
(800) 828-1628 (customer service)

email:   tim.mor...@thermofisher.com

The World Leader in Serving Science



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[Histonet] Reference or service lab that does PDGFR-a staining?

[Morken, Tim]

Hi, does anyone know of a reference or service lab that does immuno staining 
for PDGFR-a,b (platelet derived growth factor receptor alpha and/or beta.


Please note my new street address and phone number

Tim Morken
Technical Support Manager
Immunohistochemistry
Anatomical Pathology
Thermo Scientific
46360 Fremont Blvd
Fremont, CA 94538, USA

Ph: (510) 979-5000, ext 31819
or (800) 232-3342 ext 31819
Fax: (510) 979-5328
(800) 828-1628 (customer service)

email:   tim.mor...@thermofisher.com

The World Leader in Serving Science



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[Histonet] Re: IF

[Johnson, Teri]

Vanessa,

The answer of how long is suitable, the answer is "it depends".

Routinely, many will air dry over night, even over a fan, in order to achieve 
the best adherence to the glass slide. We have found that we lose 
immunoreactivity with some antigens if we let it go that long. Our routine has 
now changed to 2 hour drying time. We tried 1 hour but the researchers had 
trouble with the tissues (especially neural tissue) trying to lift off during 
the staining procedure.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110


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Re: [Histonet] IF

[Merced Leiker]

sorry, that should read "for up to a few weeks."

--On Tuesday, March 24, 2009 1:00 PM -0400 Merced Leiker 
 wrote:



We don't let our unfixed sections air-dry (room temp) long before fixing
them - a few minutes? For some people here I let them sit a few minutes
then just store them in the -80 for up to a weeks weeks before they fix
them and commence with their staining procedure. If the animals were
perfusion-fixed maybe they can air-dry longer. I've heard everything from
5 min to overnight. At room temperature.

Just my input.

--On Tuesday, March 24, 2009 12:17 PM -0400 "Vanessa J. Phelan"
 wrote:


Hi everyone,

When carrying out IF on frozen sections how long after the cutting the
sections should you leave the slides to dry and where before starting the
staining procedure?

Thanks a mill,

V

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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Re: [Histonet] IF

[Merced Leiker]

We don't let our unfixed sections air-dry (room temp) long before fixing 
them - a few minutes? For some people here I let them sit a few minutes 
then just store them in the -80 for up to a weeks weeks before they fix 
them and commence with their staining procedure. If the animals were 
perfusion-fixed maybe they can air-dry longer. I've heard everything from 5 
min to overnight. At room temperature.


Just my input.

--On Tuesday, March 24, 2009 12:17 PM -0400 "Vanessa J. Phelan" 
 wrote:



Hi everyone,

When carrying out IF on frozen sections how long after the cutting the
sections should you leave the slides to dry and where before starting the
staining procedure?

Thanks a mill,

V

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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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[Histonet] IF

[Vanessa J. Phelan]

Hi everyone,

When carrying out IF on frozen sections how long after the cutting the sections 
should you leave the slides to dry and where before starting the staining 
procedure?

Thanks a mill,

V

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RE: [Histonet] hematoxylin Question

[Swain, Frances L]

What I do Kristen if I use it infrequently is I get a large brown bottle, label 
it with working hematoxylin.  After I use the hematoxylin for whatever I am 
using it for I pour it in the brown bottle, cap it tightly and store it on the 
shelf or in a cabinet.  Whenever I need it again I get it out, set up a 
filtering stand with a funnel, filtering paper etc and filter it back into the 
dish to be used again.  You can test your hematoxylin to see if it is still 
good by dropping a drop in either distilled water or 37% formaldehyde.  It is 
turns blue purple it is still good if it turns a mauve color toss it.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfranc...@uams.edu email

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Tuesday, March 24, 2009 9:44 AM
To: histonet
Subject: [Histonet] hematoxylin Question

Hello All,
How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is 
poured into a staining container?  The stain gets used so infrequently I would 
like to keep it as long as I can.  We currently dump it weekly regardless of 
use.  Thank you.
 
-Kristen


  
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[Histonet] Thanks for all the formalin waste responses

[Jessica Piche]

 
 
Hi All I just wanted to say thanks to everyone who has responded to my question 
regarding formalin waste going down the drain. I appreciate it! 
Have a great day!

Jessica Piche-Grocki, HT(ASCP)
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AW: [Histonet] C4d Antibody

[Gudrun Lang]

Our's is from Zytomed, a polyclonal rabbit (a12-5000). We work with the
benchmark XT, 1:25 with amplifier.
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar
Francisco Romero
Gesendet: Dienstag, 24. März 2009 05:01
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] C4d Antibody


Dear People from Histonet

 

Could you share your experience with C4d Antibody ?

 

I couldn't get any results with the mouse monoclonal from Quidel.

 

I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe.

 

I work in a Public Hospital with many kidney transplantations per year.

 

Thank You in advance.

 

César Romero

Buenos Aires

Argentina

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[Histonet] hematoxylin Question

[kristen arvidson]

Hello All,
How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is 
poured into a staining container?  The stain gets used so infrequently I would 
like to keep it as long as I can.  We currently dump it weekly regardless of 
use.  Thank you.
 
-Kristen



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[Histonet] RELIA JOB ALERT Brand New Start-up Specialty Lab in Poughkeepsie NY!!

[Pam Barker]

Hi Histonetters!
I hope everyone is having a great day.  I have a new opportunity I want
to tell you about!
I am working with a brand new specialty lab opening in the Poughkeepsie
area.  They are in need of 2 histotechs 1-junior and 1 senior tech.
for full time permanent day shift positions.  The shift is 8-5 M-F and
T-S you would be alternating the Saturdays.  They are looking for people
who are experienced in routine histology including cutting, embedding
special stains -immunos are a plus.  A New York State license and ASCP
certification are not required for these jobs.  My client offers a
competitive salary, benefits and the opportunity to participate in the
start up of a brand new histology lab.  
 
If you or someone you know might be interested please contact me.  I can
be reached toll free at 866-607-3542 or rel...@earthlink.net.
 

Thank You!
 
Pam Barker
President
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
Toll Free: (866)607-3542
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
<>
www.myspace.com/pamatrelia 

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Re: [Histonet] C4d Antibody

[Angela Bitting]

We get our from Alpco.

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


>>> Cesar Francisco Romero  3/24/2009 12:00 AM
>>>

Dear People from Histonet

 

Could you share your experience with C4d Antibody ?

 

I couldn't get any results with the mouse monoclonal from Quidel.

 

I will try now the rabbit polyclonal ( C4dpAb )from Biomedica -
Grouppe.

 

I work in a Public Hospital with many kidney transplantations per
year.

 

Thank You in advance.

 

César Romero

Buenos Aires

Argentina

_
¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN
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[Histonet] Substitutes for Technovit 7100

[Jorge Tornero]

Hi all,

I am lookin for a substitute for Technovit 7100 in our research (currently
on fish female gonad). Any help or suggestions about this point would be
very very appreciated.

Best regards

Jorge Tornero
IEO - Cádiz- Spain
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