[Histonet] Leica Bond Max - any opinions?

[Michelle MacVeigh-Aloni]

Hi all,

I am researching the Leica Bond Max immunostainer and I like its features as 
advertised. 

The advertisement however states that the system is completely open, but after 
discussing it in length with the Leica rep I am finding that this is actually 
not so... 

We are a research core and work with mice and rats. Most of the pre-made 
antibody are designed towards human tests, so we won't use them. Then we would 
still be stuck with lots of money for consumables (as I understood all sold as 
kits with the antibodies included). We won't be using any of the antibodies, 
only the rest of the solutions... Is there a way out of this trap? Is there 
something that I didn't understand?

My question is: 
Does anybody use Leica Bond as an open system and use other companies antibody, 
buffers and antigen retrieval protocols? If you do, what have you done so far? 
(Would you share your protocols?) We like the BioCare products and our helpful 
rep :-) 
Did anybody purchase the Research dongle? Do we really need it? How did it help 
you improve your applications? I was told that depending on what I want 
unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is 
not a hospital where we can charge the insurance or patients for this... 

I need this info ASAP, because we have to make our decision in a day and there 
is no time to demo the unit (nor anything else).
I understand that it is great for pathology, but how about animal research? It 
costs A LOT!!! We better know what are we getting ourselves into.

 Please help! Any advise and suggestions that I can get would be highly 
appreciated.

Michelle Mac Veigh Aloni MS, HTL
USC School of Medicine 
Department of GI and Liver
Los Angeles, CA
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Re: [Histonet] onalighternote

[Va Paula Sicurello]

Hey, we can't help it if your parents were big fans of his.  He is a great song 
writer.

;-)


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Tue, 3/31/09, Bernie Taupin  wrote:

> From: Bernie Taupin 
> Subject: Re: [Histonet] onalighternote
> To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." 
> , histonet@lists.utsouthwestern.edu
> Date: Tuesday, March 31, 2009, 4:52 PM
> Yeah, hilarious.. I swear, I haven't
> heart THAT one before 
> 
> 
> 
> 
> 
> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" 
> To: "Edwards, R.E." ;
> histonet@lists.utsouthwestern.edu
> Sent: Tuesday, March 31, 2009 10:18:36 AM
> Subject: RE: [Histonet] onalighternote
> 
> I wanted to ask how Elton was but thought that was a bit
> too easy...
> 
> 
> 
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590 
> jeanine.bartl...@cdc.hhs.gov
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]
> On Behalf Of Edwards,
> R.E.
> Sent: Tuesday, March 31, 2009 10:16 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] onalighternote
> 
> 
> I have  just  had  s from  Bernie
> Taupin and Paul Schofield, is
> this  a  record??.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]
> On Behalf Of Robyn
> Vazquez
> Sent: 31 March 2009 15:08
> To: Bernie Taupin; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Ford Royer
> 
> I think Ford gets the message...GET ON WITH IT! 
> Antibody talk anyone?
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]
> On Behalf Of Bernie
> Taupin
> Sent: Tuesday, March 31, 2009 6:50 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Ford Royer
> 
> > I can personally attest that Ford must have been
> having a VERY bad day
> indeed.
> 
> Although I can empathize that this might be the case, I do
> find it
> curious that we've heard nothing from Mr. Royer since his
> little
> outburst.
> 
> I, for one, wouldn't mind an apology to the list for your
> antisocial
> behaviour on it, Mr. Royer.
> 
> 
> 
>       
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> 
> 
>       
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RE: [Histonet] Calcium salts

[Tony Henwood]

Tony,

I have been lead to believe that after von Kossa staining, Silver
carbonate dissolves in sodium thiosulphate and cannot be demonstrated
with von Kossa's technique. Though I have not confirmed this


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony
Reilly
Sent: Tuesday, 31 March 2009 4:33 PM
To: histonet
Subject: [Histonet] Calcium salts


Hello
 
I am trying to differentiate Calcium Phosphate from Calcium Carbonate
which both stain with the von Kossa method.  Is there a pretreatment for
which only one is soluble that could be used to differentiate the 2
components.
 
thanks
 
 
Tony Reilly

Chief Scientist
Anatomical Pathology
Pathology Queensland
Level 1, Building 15
Princess Alexandra Hospital
Ipswich Rd, 
Woolloongabba Q 4102
Australia
Ph: 07 32402412
Fax:07 32402930
tony_rei...@health.qld.gov.au



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[Histonet] Re: IHC automated buffers

[PALMER Jason (SVHM)]

We also make our own TBS for our Dako autostainer, same as Jan.  I have used 
PBS instead of TBS at times, when transfering a bench run where I used PBS / 
tween (my standard buffer) to the machine.  I wonder if TBS really does give 
better results than TBS, when averaged out over all Abs?  I do know that TBS 
gets fungal contamination more easily than PBS (due to the organic / oxidisable 
nature of tris) and therefore has to be replaced more frequently, which is a 
drag!
 
Jason
 
Jason Palmer
Histology Laboratory Coordinator
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.pal...@svhm.org.au

Date: Mon, 30 Mar 2009 12:48:38 -0500
From: "Jan Shivers" 
Subject: Re: [Histonet] IHC automated buffers
To: "Charles, Roger" ,

Message-ID: <13ec8a4018c84a82a43104d961f1c...@auxs.umn.edu>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original

We mix up our own, using Tween 20 at 0.05% per volume.  No problems here,
when using a Dako autostainer.

Jan Shivers
UMN Vet Diag Lab

- Original Message -
From: "Charles, Roger" 
To: 
Sent: Monday, March 30, 2009 9:53 AM
Subject: [Histonet] IHC automated buffers


Hello,
Is anyone presently making their own TBS with tween 20 buffer to be used in
automated IHC?  I would like to know if there are any problems related to
these buffers and if any deviations must be made to accommodate the
automation.

Thanks
Roger

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rchar...@state.pa.us




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RE: [Histonet] Effects of Cytocool on IHC

[Sebree Linda A]

I have heard not to use freezing spray on blocks/sections that are
destined for IHC but I never heard the reasoning behind this warning.
I've always steered clear of it when cutting for IHC and instead will
bury my block in the frost of our freezer until its good and cold.
Works fine.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leslie
McCormack
Sent: Tuesday, March 31, 2009 2:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Effects of Cytocool on IHC



In our lab many of the histotechs use Cytocool when cutting paraffin
blocks.  Has anyone heard of this having a negative effect on IHC?  

 

Leslie McCormack

Baptist Medical Center

Jacksonville, FL 32207

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Re: [Histonet] Looking for Lab

[Rene J Buesa]

Bill:
I think you refer to the National Hansen's Disease Center at the Louisiana 
State University in Baton Rouge. If you contact the LSU for sure they can tell 
you about it.
René J.

--- On Tue, 3/31/09, WILLIAM DESALVO  wrote:

From: WILLIAM DESALVO 
Subject: [Histonet] Looking for Lab
To: "histonet" 
Date: Tuesday, March 31, 2009, 4:10 PM

I have heard that there is a research lab in Baton Rouge, LA that specialises
in Leprosy cases. I have a request from one of my Derm Paths to locate so that
we can send a difficult case. I would apprciate any information. Thanks

 

William DeSalvo, B.S. HTL(ASCP)

Production Manager

Sonora Quest Laboratory 

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Re: [Histonet] Looking for Lab

[Judith L. Williams]

Hi - I think it is the Pennington Labs in BR. If you can contact Cheryl Crowder 
in BR at ccrow...@vetmed.lsu.edu
she will probably know for sure.
Judy in Washington (A tiger grad- geaux tigers!)

On Tue, 31 Mar 2009, WILLIAM DESALVO wrote:



I have heard that there is a research lab in Baton Rouge, LA that specialises 
in Leprosy cases. I have a request from one of my Derm Paths to locate so that 
we can send a difficult case. I would apprciate any information. Thanks



William DeSalvo, B.S. HTL(ASCP)

Production Manager

Sonora Quest Laboratory

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Judith Williams, PhD, HT(ASCP)
Research Scientist
Department of Comparative Medicine
University of Washington
Seattle, WA 98195



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[Histonet] Looking for Lab

[WILLIAM DESALVO]

I have heard that there is a research lab in Baton Rouge, LA that specialises 
in Leprosy cases. I have a request from one of my Derm Paths to locate so that 
we can send a difficult case. I would apprciate any information. Thanks

 

William DeSalvo, B.S. HTL(ASCP)

Production Manager

Sonora Quest Laboratory 

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[Histonet] Problems with Cutting

[Peggy Bisher]

Hi Histonetters,

I have someone here who is having problems with their samples (mouse lung
and mammary). It appears that the sample is very brittle when she cuts and
in addition, some of the paraffin around the sample in the cassette is very
white in color instead of the normal translucent color. I think it is poor
infiltration of some kind.

I told her about this great resource, this website, and I told her I would
get lots of helpful responses.

If anyone would like a picture, I could send you one.

Thank you, 



Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbis...@princeton.edu




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RE: [Histonet] (no subject)

[Jacqueline Farnsworth]

We file all our blank slides with the stained slides. That way we can always go 
back to them rather than cutting any more on an already minute piece of tissue 
remaining.  Also, for legal reasons, what if the blanks that are discarded 
contained tumor?  (or NO tumor)  IE: Showing something other than what the 
original stained slides showed.


Jacqueline Farnsworth
Anatomic Pathology, Tech III
Foothills Medical Centre
Calgary Laboratory Services

Ph: 403-944-1578
Fax: 403-944-4748
P Please consider the environment before printing this email.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun 
[rcar...@harthosp.org]
Sent: March 31, 2009 1:45 PM
To: histonet@lists.utsouthwestern.edu; debra.or...@uchospitals.edu
Subject: Re: [Histonet] (no subject)

Here at Hartford Hospital we do the following for biopsies:

Prostate:  5 slides (1, 3, and 5 - H&E/2 and 4 - unstain)

Lung:  5 slides (1, 3, and 5 - H&E/2 and 4 - unstain)

Breast:  7 slides (1, 4, and 7 - H&E/ 2 ,3, 5, and 6 - unstain)

Transplant heart:  8 slides (1, 3, 5, and 7 - H&E/2, 4, 6, and 8 - unstain)

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>>  3/31/2009 10:30 AM >>>
We are currently handling all prostate, lung, heart, and breast biopsies
as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for
H&E's and the rest as unstained plus slides. What has happened is that
we throw thousands of unused expensive slides a year because they are
not used. Is there a general standard in practice out there for FNBs?
Unfortunately, with the economy the way it is; and trying always to find
ways to bring down expenses this seems counteractive.



Thank you

Debi



Debra Ann Ortiz

Chief Medical Technologist

The University of Chicago Medical Center

Room E-602-A

5841 S. Maryland Avenue

Chicago, Il 60637

phone: 773.702.5237




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Thank you
University of Chicago Medical Center

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Re: [Histonet] (no subject)

[Richard Cartun]

Here at Hartford Hospital we do the following for biopsies:

Prostate:  5 slides (1, 3, and 5 - H&E/2 and 4 - unstain)

Lung:  5 slides (1, 3, and 5 - H&E/2 and 4 - unstain)

Breast:  7 slides (1, 4, and 7 - H&E/ 2 ,3, 5, and 6 - unstain)

Transplant heart:  8 slides (1, 3, 5, and 7 - H&E/2, 4, 6, and 8 - unstain)

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>>  3/31/2009 10:30 AM >>>
We are currently handling all prostate, lung, heart, and breast biopsies
as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for
H&E's and the rest as unstained plus slides. What has happened is that
we throw thousands of unused expensive slides a year because they are
not used. Is there a general standard in practice out there for FNBs?
Unfortunately, with the economy the way it is; and trying always to find
ways to bring down expenses this seems counteractive.

 

Thank you

Debi

 

Debra Ann Ortiz

Chief Medical Technologist

The University of Chicago Medical Center

Room E-602-A

5841 S. Maryland Avenue

Chicago, Il 60637

phone: 773.702.5237

 


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Thank you
University of Chicago Medical Center 

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[Histonet] Histology Training

[Poole, Kimberly]

Hi,

 

Does anyone know of a place that I can get histology training? I am 
specifically looking for a place in the US or Canada to go to for a couple of 
weeks. I am not able to take courses that are 1 to 2 years long. Thanks in 
advance!!

 

Kimberly Poole B.Sc

Casualty Management Section | Section de la gestion des blessés

Defence Research and Development Canada Suffield | Recherche et développement 
pour la défense Canada Suffield 

Medicine Hat, AB, Canada T1A 8K6

kimberly.po...@drdc-rddc.gc.ca  

Telephone | Téléphone 403-544-5347 / Facsimile | Télécopieur 403-544-4714 

Government of Canada | Gouvernement du Canada

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Re: [Histonet] AO Blade holder

[Rene J Buesa]

With the lever loose, tight the two screws in a way that the blade is free and 
can be moved in any direction. The lever will lock the blade and it should be 
tighten "all the way".
René J.

--- On Tue, 3/31/09, David Waugh  wrote:

From: David Waugh 
Subject: [Histonet] AO Blade holder
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 31, 2009, 3:08 PM

> I have a question regarding a AO disposable blade holder (Cat# 824). This
link should show a picture of a similar one
www.usedmicroscopes.com/images/AO820Microtome.jpg
> 
> The blade is tightened with a lever on the right that rotates a d-shaped
cam. Two screws are used the set the tension that the cam places on the blade,
once set, the cam will tighten and loosen the blade. The back of the holder is
concave, and the front part convex, so that when tightened, the blade will bow.
I have not been able to find any documentation on how tight the blade should be.
It sounds like most holders have flat surfaces that clamp the blade, which I
would think would have very different tightness requirements. Any ideas from
someone who uses this type of holder on how the two screws should be set?
Thanks, David
> 
> Kent State University
> Department of Geology
> Kent, Ohio 44242
> dwa...@kent.edu
> 


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[Histonet] Effects of Cytocool on IHC

[Leslie McCormack]

In our lab many of the histotechs use Cytocool when cutting paraffin blocks.  
Has anyone heard of this having a negative effect on IHC?  

 

Leslie McCormack

Baptist Medical Center

Jacksonville, FL 32207

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RE: [Histonet] AO Blade holder

[Swain, Frances L]

Hi David:   When I used an AO with a disposable blade holder.  I had to test a 
blank paraffin block to get the blade the right tightness.  There was no set 
tension that I can remember.  What I would do it tighten the blade holder until 
the disposable blade was held securely.  Test the knife with the blank block.  
If the blade was too tight I would loosen the lever and back the screws off 1/4 
turn.  Tighten it back up and test it again.  I would continue doing this until 
I got the right tension.  It really sounds complicated but it really isn't  
just make sure that the springs are good and the lever is clean.  Once you get 
it set you are good to go until you clean your holder and then you have to go 
through the procedure again.   This is the best that I can remember.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfranc...@uams.edu email

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David Waugh
Sent: Tuesday, March 31, 2009 2:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AO Blade holder



> I have a question regarding a AO disposable blade holder (Cat# 824).  
> This link should show a picture of a similar one 
> www.usedmicroscopes.com/images/AO820Microtome.jpg
>
> The blade is tightened with a lever on the right that rotates a d- 
> shaped cam. Two screws are used the set the tension that the cam  
> places on the blade, once set, the cam will tighten and loosen the  
> blade. The back of the holder is concave, and the front part convex,  
> so that when tightened, the blade will bow. I have not been able to  
> find any documentation on how tight the blade should be. It sounds  
> like most holders have flat surfaces that clamp the blade, which I  
> would think would have very different tightness requirements. Any  
> ideas from someone who uses this type of holder on how the two  
> screws should be set? Thanks, David
>
> Kent State University
> Department of Geology
> Kent, Ohio 44242
> dwa...@kent.edu
>


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[Histonet] AO Blade holder

[David Waugh]

I have a question regarding a AO disposable blade holder (Cat# 824).  
This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg


The blade is tightened with a lever on the right that rotates a d- 
shaped cam. Two screws are used the set the tension that the cam  
places on the blade, once set, the cam will tighten and loosen the  
blade. The back of the holder is concave, and the front part convex,  
so that when tightened, the blade will bow. I have not been able to  
find any documentation on how tight the blade should be. It sounds  
like most holders have flat surfaces that clamp the blade, which I  
would think would have very different tightness requirements. Any  
ideas from someone who uses this type of holder on how the two  
screws should be set? Thanks, David


Kent State University
Department of Geology
Kent, Ohio 44242
dwa...@kent.edu




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[Histonet] Online SOP's

[Bauer, Karen L.]

Hello,
 
Does anyone have only procedures online?  All of ours are online, but we
still keep a paper copy of each in a manual in the lab.  I would like to
get rid of the manual, but then how do you record pathologist sign-off
on all new or revised procedures?
 
I'm curious to hear what other labs are doing.
 
Thanks much,
 
Karen
 
Karen L. Bauer HT(ASCP), BS
Histology Section Chief
Department of Pathology - Luther Hospital
Luther Midelfort - Mayo Health System
715-838-3205
bauer.ka...@mayo.edu
 
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[Histonet] AO Blade holder

[David Waugh]

I have a question regarding a AO disposable blade holder (Cat# 824).  
This link should show a picture of a similar one  
www.usedmicroscopes.com/images/AO820Microtome.jpg


The blade is tightened with a lever on the right that rotates a d- 
shaped cam. Two screws are used the set the tension that the cam  
places on the blade, once set, the cam will tighten and loosen the  
blade. The back of the holder is concave, and the front part convex,  
so that when tightened, the blade will bow. I have not been able to  
find any documentation on how tight the blade should be. It sounds  
like most holders have flat surfaces that clamp the blade, which I  
would think would have very different tightness requirements. Any  
ideas from someone who uses this type of holder on how the two screws  
should be set? Thanks, David


Kent State University
Department of Geology
Kent, Ohio 44242
dwa...@kent.edu


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[Histonet] AO disposable blade holder

[David Waugh]

I have a question regarding a AO disposable blade holder (Cat# 824).  
This link should show a picture of a similar one (http:// 
www.usedmicroscopes.com/images/AO820Microtome.jpg)


The blade is tightened with a lever on the right that rotates a d- 
shaped cam. Two screws are used the set the tension that the cam  
places on the blade, once set, the cam will tighten and loosen the  
blade. The back of the holder is concave, and the front part convex,  
so that when tightened, the blade will bow. I have not been able to  
find any documentation on how tight the blade should be. It sounds  
like most holders have flat surfaces that clamp the blade, which I  
would think would have very different tightness requirements. Any  
ideas from someone who uses this type of holder on how the two screws  
should be set? Thanks, David


Kent State University
Department of Geology
Kent, Ohio 44242
dwa...@kent.edu
www.personal.kent.edu/~dwaugh



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[Histonet] RE: cutting protocols with extra slides

[Terri Braud]

Hi Debi - 
I've seen protocols similar to what you describe, and in my experience, they 
are almost always a result of someone, at some point in time, cutting through 
(wasting) a critical small specimen after the initial H&Es were cut or during 
multiple levels. Perhaps the case can be made that you can insure that it will 
not happen?  Maybe offer to separate the protocols by tissue type...for 
example, try to reduce or eliminate extra slides for less critical or larger 
needle biopsies such as prostate, breast, etc., and keep it for tiny or 
difficult specimens such as cardiac biopsies, In some labs, even 3 H&E's would 
be considered excessive.  Best of luck to you in your quest.
Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital and Medical Center
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3676 phone
(215) 938-3689 fax


Message: 17
Date: Tue, 31 Mar 2009 09:30:18 -0500
From: 
We are currently handling all prostate, lung, heart, and breast biopsies
as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for
H&E's and the rest as unstained plus slides. What has happened is that
we throw thousands of unused expensive slides a year because they are
not used. Is there a general standard in practice out there for FNBs?
Unfortunately, with the economy the way it is; and trying always to find
ways to bring down expenses this seems counteractive.
Thank you
Debi
Debra Ann Ortiz
Chief Medical Technologist
The University of Chicago Medical Center
Room E-602-A
5841 S. Maryland Avenue
Chicago, Il 60637
phone: 773.702.5237


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[Histonet] AO disposable blade holder

[David Waugh]

I have a question regarding a AO disposable blade holder (Cat# 824).  
This link should show a picture of a similar one (http:// 
www.usedmicroscopes.com/images/AO820Microtome.jpg)


The blade is tightened with a lever on the right that rotates a d- 
shaped cam. Two screws are used the set the tension that the cam  
places on the blade, once set, the cam will tighten and loosen the  
blade. The back of the holder is concave, and the front part convex,  
so that when tightened, the blade will bow. I have not been able to  
find any documentation on how tight the blade should be. It sounds  
like most holders have flat surfaces that clamp the blade, which I  
would think would have very different tightness requirements. Any  
ideas from someone who uses this type of holder on how the two screws  
should be set? Thanks, David


Kent State University
Department of Geology
Kent, Ohio 44242
dwa...@kent.edu
www.personal.kent.edu/~dwaugh



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RE: [Histonet] microwave tissue processor

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

We have had great success with our Sakura Xpress. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Tuesday, March 31, 2009 11:45 AM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] microwave tissue processor

We are thinking about Microwave Tissue processors.  Unfortunately I have
been given very little time to present a budget request.  Can anyone
comment on a recent purchase of a microwave tissue processor and their
experiences?

Thanks




Jim Vickroy BS, HT(ASCP)
Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical
Center
217-788-4046
vickroy@mhsil.com



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Re: [Histonet] onalighternote

[Bernie Taupin]

Yeah, hilarious. I swear, I haven't heart THAT one before 





From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" 
To: "Edwards, R.E." ; histonet@lists.utsouthwestern.edu
Sent: Tuesday, March 31, 2009 10:18:36 AM
Subject: RE: [Histonet] onalighternote

I wanted to ask how Elton was but thought that was a bit too easy...



Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards,
R.E.
Sent: Tuesday, March 31, 2009 10:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] onalighternote


I have  just  had  s from  Bernie Taupin and Paul Schofield, is
this  a  record??.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn
Vazquez
Sent: 31 March 2009 15:08
To: Bernie Taupin; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ford Royer

I think Ford gets the message...GET ON WITH IT!  Antibody talk anyone?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie
Taupin
Sent: Tuesday, March 31, 2009 6:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ford Royer

> I can personally attest that Ford must have been having a VERY bad day
indeed.

Although I can empathize that this might be the case, I do find it
curious that we've heard nothing from Mr. Royer since his little
outburst.

I, for one, wouldn't mind an apology to the list for your antisocial
behaviour on it, Mr. Royer.



  
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RE: [Histonet] microwave tissue processor

[Joyce Cline]

Milestone makes excellent microwave processors for small GI biopsies and for
large tissues.

Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Tuesday, March 31, 2009 11:45 AM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] microwave tissue processor

We are thinking about Microwave Tissue processors.  Unfortunately I have
been given very little time to present a budget request.  Can anyone comment
on a recent purchase of a microwave tissue processor and their experiences?

Thanks




Jim Vickroy BS, HT(ASCP)
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
217-788-4046
vickroy@mhsil.com



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[Histonet] microwave tissue processor

[Vickroy, Jim]

We are thinking about Microwave Tissue processors.  Unfortunately I have been 
given very little time to present a budget request.  Can anyone comment on a 
recent purchase of a microwave tissue processor and their experiences?

Thanks




Jim Vickroy BS, HT(ASCP)
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
217-788-4046
vickroy@mhsil.com



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Re: [Histonet] free to good home AO 820 microtome

[Caroline Miller]

Yes please!!! I am desperate for a new microtome!!!

Caroline


Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158

www.gladstone.ucsf.edu

On Mar 31, 2009, at 6:29, "Cathy Mayton"  wrote:

I have an AO 820 microtome with gray cover free to a good.  It still  
works great and was replaced with a new microtome we used in the  
lab.  Has been sitting idle with a dust cover for many years.  Just  
pay the shipping to its new home.



Cathy A. Mayton
Wasatch Histo Consultants, Inc.
80 Youngberg Road
Winnemucca, NV 89445
775-625-4425
www.wasatchhisto.com
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RE: [Histonet] (no subject)

[Podawiltz, Thomas]

We do not throw them out, we file them with the stained slides for future 
reference.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V 
[hor...@archildrens.org]
Sent: Tuesday, March 31, 2009 10:50 AM
To: debra.or...@uchospitals.edu; corne...@hotmail.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

I agree with Lorraine on this one.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
debra.or...@uchospitals.edu
Sent: Tuesday, March 31, 2009 9:43 AM
To: corne...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

Thank you for your very prompt response. I do agree that we are here for
the patient.

Unfotunately I am under the gun to bring down costs and I thought that
might be one way. Thanks again, and look forward to other responses.



Debi





From: Lorraine Cornett [mailto:corne...@hotmail.com]
Sent: Tuesday, March 31, 2009 9:36 AM
To: Ortiz, Debra [UCH]
Subject: RE: [Histonet] (no subject)



Yes,
Unfortunately(financially) it is current practice at our facility as
well.  As a preemptive measure, I keep a record of the number of all
unstained slides(total number only) thrown out on a monthly basis.  The
only alternative is to not cut the extra's and take a chance on not
getting the proper area on the slide for diagnostic stains.  I think
that patient care is of the utmost importance, and this probably is a
small price to pay in the grand scheme of things.


Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division,
Kingsport, TN 423 224-5793 fax 423 224-5349



> Date: Tue, 31 Mar 2009 09:30:18 -0500
> From: debra.or...@uchospitals.edu
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> We are currently handling all prostate, lung, heart, and breast
biopsies
> as fine needle biopsies (FNB). We cut 10 slides 3 of which are used
for
> H&E's and the rest as unstained plus slides. What has happened is that
> we throw thousands of unused expensive slides a year because they are
> not used. Is there a general standard in practice out there for FNBs?
> Unfortunately, with the economy the way it is; and trying always to
find
> ways to bring down expenses this seems counteractive.
>
>
>
> Thank you
>
> Debi
>
>
>
> Debra Ann Ortiz
>
> Chief Medical Technologist
>
> The University of Chicago Medical Center
>
> Room E-602-A
>
> 5841 S. Maryland Avenue
>
> Chicago, Il 60637
>
> phone: 773.702.5237
>
>
>
>


> This e-mail is intended only for the use of the individual or entity
to which
> it is addressed and may contain information that is privileged and
confidential.
> If the reader of this e-mail message is not the intended recipient,
you are
> hereby notified that any dissemination, distribution or copying of
this
> communication is prohibited. If you have received this e-mail in
error, please
> notify the sender and destroy all copies of the transmittal.
>
> Thank you
> University of Chicago Medical Center
>


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*

RE: [Histonet] (no subject)

[Horn, Hazel V]

I agree with Lorraine on this one. 

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax501.364.3155
 
visit us on the web at:www.archildrens.org
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
debra.or...@uchospitals.edu
Sent: Tuesday, March 31, 2009 9:43 AM
To: corne...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

Thank you for your very prompt response. I do agree that we are here for
the patient. 

Unfotunately I am under the gun to bring down costs and I thought that
might be one way. Thanks again, and look forward to other responses.

 

Debi

 



From: Lorraine Cornett [mailto:corne...@hotmail.com] 
Sent: Tuesday, March 31, 2009 9:36 AM
To: Ortiz, Debra [UCH]
Subject: RE: [Histonet] (no subject)

 

Yes,
Unfortunately(financially) it is current practice at our facility as
well.  As a preemptive measure, I keep a record of the number of all
unstained slides(total number only) thrown out on a monthly basis.  The
only alternative is to not cut the extra's and take a chance on not
getting the proper area on the slide for diagnostic stains.  I think
that patient care is of the utmost importance, and this probably is a
small price to pay in the grand scheme of things.


Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division,
Kingsport, TN 423 224-5793 fax 423 224-5349


 
> Date: Tue, 31 Mar 2009 09:30:18 -0500
> From: debra.or...@uchospitals.edu
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
> 
> We are currently handling all prostate, lung, heart, and breast
biopsies
> as fine needle biopsies (FNB). We cut 10 slides 3 of which are used
for
> H&E's and the rest as unstained plus slides. What has happened is that
> we throw thousands of unused expensive slides a year because they are
> not used. Is there a general standard in practice out there for FNBs?
> Unfortunately, with the economy the way it is; and trying always to
find
> ways to bring down expenses this seems counteractive.
> 
> 
> 
> Thank you
> 
> Debi
> 
> 
> 
> Debra Ann Ortiz
> 
> Chief Medical Technologist
> 
> The University of Chicago Medical Center
> 
> Room E-602-A
> 
> 5841 S. Maryland Avenue
> 
> Chicago, Il 60637
> 
> phone: 773.702.5237
> 
> 
> 
>


> This e-mail is intended only for the use of the individual or entity
to which
> it is addressed and may contain information that is privileged and
confidential.
> If the reader of this e-mail message is not the intended recipient,
you are 
> hereby notified that any dissemination, distribution or copying of
this
> communication is prohibited. If you have received this e-mail in
error, please 
> notify the sender and destroy all copies of the transmittal. 
> 
> Thank you
> University of Chicago Medical Center 
>


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notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 


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**
The i

[Histonet] staining of fibrin, muscle, collagen and elastic fiber

[ooi . ting . huay]

   Hi,  I  am  interested  to look at the fibrin, muscle, collagen and e   
lastic  fiber distribution in the artery in the plastic resin section.
   Any  s=  uggestion  for the specific staining that is required? I know
   that  Movat  pen=  tachrome  will  stain  muscle, collagen and elastic
   fiber.  Do  anyone know the = staining for fibrin? Or is there any kit
   that  is  available  for  all  the stai= ning above? Or any suggestion
   regarding the staining for the desired stain?= Thank you very much!
   Regards,
   Ooi

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RE: [Histonet] (no subject)

[Debra.Ortiz]

Thank you for your very prompt response. I do agree that we are here for
the patient. 

Unfotunately I am under the gun to bring down costs and I thought that
might be one way. Thanks again, and look forward to other responses.

 

Debi

 



From: Lorraine Cornett [mailto:corne...@hotmail.com] 
Sent: Tuesday, March 31, 2009 9:36 AM
To: Ortiz, Debra [UCH]
Subject: RE: [Histonet] (no subject)

 

Yes,
Unfortunately(financially) it is current practice at our facility as
well.  As a preemptive measure, I keep a record of the number of all
unstained slides(total number only) thrown out on a monthly basis.  The
only alternative is to not cut the extra's and take a chance on not
getting the proper area on the slide for diagnostic stains.  I think
that patient care is of the utmost importance, and this probably is a
small price to pay in the grand scheme of things.


Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division,
Kingsport, TN 423 224-5793 fax 423 224-5349


 
> Date: Tue, 31 Mar 2009 09:30:18 -0500
> From: debra.or...@uchospitals.edu
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
> 
> We are currently handling all prostate, lung, heart, and breast
biopsies
> as fine needle biopsies (FNB). We cut 10 slides 3 of which are used
for
> H&E's and the rest as unstained plus slides. What has happened is that
> we throw thousands of unused expensive slides a year because they are
> not used. Is there a general standard in practice out there for FNBs?
> Unfortunately, with the economy the way it is; and trying always to
find
> ways to bring down expenses this seems counteractive.
> 
> 
> 
> Thank you
> 
> Debi
> 
> 
> 
> Debra Ann Ortiz
> 
> Chief Medical Technologist
> 
> The University of Chicago Medical Center
> 
> Room E-602-A
> 
> 5841 S. Maryland Avenue
> 
> Chicago, Il 60637
> 
> phone: 773.702.5237
> 
> 
> 
>


> This e-mail is intended only for the use of the individual or entity
to which
> it is addressed and may contain information that is privileged and
confidential.
> If the reader of this e-mail message is not the intended recipient,
you are 
> hereby notified that any dissemination, distribution or copying of
this
> communication is prohibited. If you have received this e-mail in
error, please 
> notify the sender and destroy all copies of the transmittal. 
> 
> Thank you
> University of Chicago Medical Center 
>


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This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, please 
notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 

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[Histonet] (no subject)

[Debra.Ortiz]

We are currently handling all prostate, lung, heart, and breast biopsies
as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for
H&E's and the rest as unstained plus slides. What has happened is that
we throw thousands of unused expensive slides a year because they are
not used. Is there a general standard in practice out there for FNBs?
Unfortunately, with the economy the way it is; and trying always to find
ways to bring down expenses this seems counteractive.

 

Thank you

Debi

 

Debra Ann Ortiz

Chief Medical Technologist

The University of Chicago Medical Center

Room E-602-A

5841 S. Maryland Avenue

Chicago, Il 60637

phone: 773.702.5237

 


This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, please 
notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 

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RE: [Histonet] onalighternote

[O'Donnell, Bill]

I think you meant to say CD. :)

Have a grand day!

Bill 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards,
R.E.
Sent: Tuesday, March 31, 2009 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] onalighternote


I have  just  had  s from  Bernie Taupin and Paul Schofield, is
this  a  record??.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn
Vazquez
Sent: 31 March 2009 15:08
To: Bernie Taupin; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ford Royer

I think Ford gets the message...GET ON WITH IT!  Antibody talk anyone?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie
Taupin
Sent: Tuesday, March 31, 2009 6:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ford Royer

> I can personally attest that Ford must have been having a VERY bad day
indeed.

Although I can empathize that this might be the case, I do find it
curious that we've heard nothing from Mr. Royer since his little
outburst.

I, for one, wouldn't mind an apology to the list for your antisocial
behaviour on it, Mr. Royer.



  
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RE: [Histonet] onalighternote

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

I wanted to ask how Elton was but thought that was a bit too easy...



Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards,
R.E.
Sent: Tuesday, March 31, 2009 10:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] onalighternote


I have  just  had  s from  Bernie Taupin and Paul Schofield, is
this  a  record??.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn
Vazquez
Sent: 31 March 2009 15:08
To: Bernie Taupin; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ford Royer

I think Ford gets the message...GET ON WITH IT!  Antibody talk anyone?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie
Taupin
Sent: Tuesday, March 31, 2009 6:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ford Royer

> I can personally attest that Ford must have been having a VERY bad day
indeed.

Although I can empathize that this might be the case, I do find it
curious that we've heard nothing from Mr. Royer since his little
outburst.

I, for one, wouldn't mind an apology to the list for your antisocial
behaviour on it, Mr. Royer.



  
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[Histonet] onalighternote

[Edwards, R.E.]

I have  just  had  s from  Bernie Taupin and Paul Schofield, is  this  a  
record??.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez
Sent: 31 March 2009 15:08
To: Bernie Taupin; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ford Royer

I think Ford gets the message...GET ON WITH IT!  Antibody talk anyone?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin
Sent: Tuesday, March 31, 2009 6:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ford Royer

> I can personally attest that Ford must have been having a VERY bad day indeed.

Although I can empathize that this might be the case, I do find it curious that 
we've heard nothing from Mr. Royer since his little outburst.

I, for one, wouldn't mind an apology to the list for your antisocial behaviour 
on it, Mr. Royer.



  
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RE: [Histonet] Ford Royer

[Robyn Vazquez]

I think Ford gets the message...GET ON WITH IT!  Antibody talk anyone?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin
Sent: Tuesday, March 31, 2009 6:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ford Royer

> I can personally attest that Ford must have been having a VERY bad day indeed.

Although I can empathize that this might be the case, I do find it curious that 
we've heard nothing from Mr. Royer since his little outburst.

I, for one, wouldn't mind an apology to the list for your antisocial behaviour 
on it, Mr. Royer.



  
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[Histonet] Ford Royer

[Bernie Taupin]

> I can personally attest that Ford must have been having a VERY bad day indeed.

Although I can empathize that this might be the case, I do find it curious that 
we've heard nothing from Mr. Royer since his little outburst.

I, for one, wouldn't mind an apology to the list for your antisocial behaviour 
on it, Mr. Royer.



  
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[Histonet] free to good home AO 820 microtome

[Cathy Mayton]

I have an AO 820 microtome with gray cover free to a good.  It still works 
great and was replaced with a new microtome we used in the lab.  Has been 
sitting idle with a dust cover for many years.  Just pay the shipping to its 
new home. 


Cathy A. Mayton
Wasatch Histo Consultants, Inc.
80 Youngberg Road
Winnemucca, NV 89445
775-625-4425
www.wasatchhisto.com
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[Histonet] PDGFR

[Santiago, Jerry]

Any lab performing PDGFR (Platelet Derived Growth Factor Receptors)? 

 

Please contact me directly.

 

jerry.santi...@jax.ufl.edu

 

Jerry Santiago

Shands Jacksonville

Jacksonville, Florida

904-244-6149

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[Histonet] opinion please

[Rene J Buesa]

Michelle:
The motorized microtome was introduced specifically to alleviate the 
carpal-tunnel syndrome quite frequent in histotechs that have been sectioning 
for years.
It is very advantageous and can take care of the trimming necessary to reach 
the area that is going to be sectioned and even then it can take care of 
sectioning for which it has different speed modes. Another advantage is that 
the rhythm is aways constant and can be regulated.
They are a good investment.
René J.

--- On Tue, 3/31/09, Michelle Perrins  wrote:

From: Michelle Perrins 
Subject: [Histonet] opinion please
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 31, 2009, 8:06 AM

Hi
I would like your opinion of the manual vs semi/fully automated rotary
microtome, especially with regard to stress on the hands.
Thanks
Michelle 
 
 
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine 
Faculty Health Sciences
University of Cape Town
 
tel: +27 21 406 6001
fax: +27 21 448 1249
Email: michelle.perr...@uct.ac.za
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[Histonet] opinion please

[Michelle Perrins]

Hi
I would like your opinion of the manual vs semi/fully automated rotary 
microtome, especially with regard to stress on the hands.
Thanks
Michelle 
 
 
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine 
Faculty Health Sciences
University of Cape Town
 
tel: +27 21 406 6001
fax: +27 21 448 1249
Email: michelle.perr...@uct.ac.za
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