RE: [Histonet] Turnbull's Blue

[Lee & Peggy Wenk]

Turnball blue (i.e., Schmorl stain) demonstrates argentaffinic components,
such as melanin or argentaffin cells in the GI tract.

Prussian blue (i.e., the iron stain) demonstrates iron (hemosiderin).

What are you trying to demonstrate? 

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospitals
Royal Oak, MI 48073

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wahlberg,
Nikki
Sent: Thursday, May 07, 2009 1:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Turnbull's Blue

Is anyone performing the Turnbull's Blue Iron stain?  If so, what type of
control do you use.  We are having a difficult time finding a good control
in order to get the stain approved in our lab.  Also, if anyone has some
tips for the procedure they would be greatly appreciated.
 
Thank you
 
Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlb...@bsci.com

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RE: [Histonet] NADPH-diaphorase

[Tony Henwood]

Eab,

We purchase our NADH (reduced form) Cat No. N8129 from Sigma-aldrich

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breisch,
Eric
Sent: Friday, 8 May 2009 2:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] NADPH-diaphorase


Hi, hoping that someone might be able to share with us where they
purchase NADPH-diaphorase for the NADPH stain. Thank you.

 

EAB

 

 

 

 

 

Eric A. Breisch, Ph.D.

Clinical Anatomist

Dept. of Pathology

Rady Children's Hospital and Health Center

Associate Clinical Professor of Anatomy

Dept. of Surgery

UCSD School of Medicine 

 

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[Histonet] disposal of body parts

[Carol Bryant]

Hello Histonetters!

I work in a small multispecialty clinic laboratory where the majority of
our specimens are derm, gi biopsies, etc.  Today we had an amputated
finger sent for gross only from our ambulatory surgery center and the
patient wishes to have it back.  Does CAP have any specific requirements
in regard to disposal of tissue that would apply here?  Don't some
patients wish to have their amputated limbs back for religious reasons?
If so, what is the proper protocol?  Should they sign some type of
release form?

Thanks,

Carol 

 

Carol Bryant, CT (ASCP)

Cytology/Histology Manager

Pathology Services

Lexington Clinic

Phone (859) 258-4082

Fax (859) 258-4081

cb...@lexclin.com

 



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Re: [Histonet] pinning specimens for fixation

[Bryan Llewellyn]

Try coroplast panels from your local home renovation/hardware store and cut 
to the size you want with a utility knife.  Also, make sure that the surgeon 
uses plastic pins, not steel, to avoid rust.  Since they will probably 
float, turn them over so the tissue is in the fixative.


Bryan


- Original Message - 
From: "Bryan Watson" 

To: 
Sent: Thursday, May 07, 2009 10:49 AM
Subject: [Histonet] pinning specimens for fixation


We have a surgeon who likes to send us specimens that are pinned down to a 
board of some sort. He uses whatever makeshift board he can find and he 
wants us to order something for that purpose. Does anybody use anything like 
this something strong enough to hold a specimen pinned down without the pins 
being pulled out that is also disposable? If so, can you tell me what 
they're called and where to get them, thanks. I'll take any alternate ideas 
as well.

Thanks,
Bryan
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Re: [Histonet] Gram & AFB staining FNA smears

[Maxim Peshkov]

Angela:
We stains our cytology slides for Gram and AFB with
the same reagents as histology. Only misses dewax and
final dehydration, clearing and coverslip steps.
Results are like both, the pathologists and
cytopathologist.
Maxim Peshkov,
Russia,
Taganrog.
  mailto:maxim...@mail.ru


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Re: [Histonet] pinning specimens for fixation

[Judith L. Williams]

HI Bryan -
In biology laboratories they use metal trays filled with a wax like product. You can make 
your own with a 9x12 metal baking dish filled half full with paraffin. Or, you can order 
the "professional" ones from Carolina Biological Supply, cheaper than from the 
anatomic path catalogs. They also have the large T-shape pins.
good luck with the dissection and fixation!
Judy


On Thu, 7 May 2009, Bryan Watson wrote:


We have a surgeon who likes to send us specimens that are pinned down to a 
board of some sort. He uses whatever makeshift board he can find and he wants 
us to order something for that purpose. Does anybody use anything like this 
something strong enough to hold a specimen pinned down without the pins being 
pulled out that is also disposable? If so, can you tell me what they're called 
and where to get them, thanks. I'll take any alternate ideas as well.
Thanks,
Bryan
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Judith Williams, PhD, HT(ASCP)
Research Scientist
Department of Comparative Medicine
University of Washington
Seattle, WA 98195



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RE: [Histonet] pinning specimens for fixation

[Garcia, Lori, Sr. Scientist]

 Hi Bryan,

Mopec makes disposable cutting boards that can be cut to the right size.
Here is the link:
http://www.mopec.com/product/626/small_disposable_cutting_boards/

Also, thick cork board works very well.

Lori

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bryan
Watson
Sent: Thursday, May 07, 2009 10:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] pinning specimens for fixation

We have a surgeon who likes to send us specimens that are pinned down to
a board of some sort. He uses whatever makeshift board he can find and
he wants us to order something for that purpose. Does anybody use
anything like this something strong enough to hold a specimen pinned
down without the pins being pulled out that is also disposable? If so,
can you tell me what they're called and where to get them, thanks. I'll
take any alternate ideas as well.
Thanks,
Bryan
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[Histonet] Holding Cell Lines in Methanol

[secash]

We are doing a 5 day Hypoxia Chamber Experiment on Cancer  Cell Lines
Slides. They are fixed in methanol at -20c for 20 minutes when they come
out.  If I cannot do the experiment at that time (due to a funeral), can
these slides be stored in the methanol at -20c overnight without it
adversely harming the cells? Thanks for the help!!

Steven Cash, BS, HT
Supervisor Oncology Research Lab
Gundersen Lutheran Medical Foundation
1300 Badger St
LaCrosse, WI 54601
608-775-3548
Mail Stop  UWL-002


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[Histonet] pinning specimens for fixation

[Bryan Watson]

We have a surgeon who likes to send us specimens that are pinned down to a 
board of some sort. He uses whatever makeshift board he can find and he wants 
us to order something for that purpose. Does anybody use anything like this 
something strong enough to hold a specimen pinned down without the pins being 
pulled out that is also disposable? If so, can you tell me what they're called 
and where to get them, thanks. I'll take any alternate ideas as well.
Thanks,
Bryan
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[Histonet] Turnbull's Blue

[Wahlberg, Nikki]

Is anyone performing the Turnbull's Blue Iron stain?  If so, what type
of control do you use.  We are having a difficult time finding a good
control in order to get the stain approved in our lab.  Also, if anyone
has some tips for the procedure they would be greatly appreciated.
 
Thank you
 
Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlb...@bsci.com

CONFIDENTIALITY NOTICE:  This e-mail transmission may contain
confidential or legally privileged information that is intended only for
the individual or entity named in the e-mail address.  Any unauthorized
distribution or copying of this transmittal or its attachments, if any,
is prohibited.  If you have received this e-mail transmission in error,
please reply to the sender, so that Boston Scientific Corporation can
arrange for proper delivery, then please delete the message from your
inbox.

 

 
 
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RE: Make a Tiny URL of that thing...RE: [Histonet] Microtome Alignment

[Martin, Gary]

Thank you Tim ... very cool! 
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
Tim
Sent: Thursday, May 07, 2009 10:10 AM
To: histonet
Subject: Make a Tiny URL of that thing...RE: [Histonet] Microtome
Alignment


Victor wrote:

"Go to Ace Hardware and get each employee one of these...

http://www.acehardware.com/product/index.jsp?productId=1286367&cp=&fbc=1
&pg=3&kw=level&lmdn=Price+Range&fbn=StorePrice%7CUnder+%2425.00&fr=Store
Price%2FACE%2F%2F2500&parentPage=search&searchId=37640061873
"



You all need to learn about TinyURL.com. You can paste in a large URL
and it makes tiny one that is easy to use in emails. Sometimes the long
ones get cut up in email and don't work

Here is the same link as above, but after using tinyURL.com:

http://tinyurl.com/d9y3s9




Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 


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Make a Tiny URL of that thing...RE: [Histonet] Microtome Alignment

[Morken, Tim]

Victor wrote:

"Go to Ace Hardware and get each employee one of these...

http://www.acehardware.com/product/index.jsp?productId=1286367&cp=&fbc=1&pg=3&kw=level&lmdn=Price+Range&fbn=StorePrice%7CUnder+%2425.00&fr=StorePrice%2FACE%2F%2F2500&parentPage=search&searchId=37640061873
 "



You all need to learn about TinyURL.com. You can paste in a large URL and it 
makes tiny one that is easy to use in emails. Sometimes the long ones get cut 
up in email and don't work

Here is the same link as above, but after using tinyURL.com:

http://tinyurl.com/d9y3s9




Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 


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Re: [Histonet] FW: prover' pojaluista

[Rene J Buesa]

Strictly speaking, IHC reactions do not depend on water temperature 
temperature. What you describe does not include dist. water between the IHC 
steps therefore the water temp. should not be affecting your reactions.
If you use dist water after HIER you have to remember placing the slides into 
room temp. buffer before starting the IHC procedure, so if there was any effect 
due to the dist. water, that should have take care of it.
The inconsistency in your staining I think is not due to the dist. water temp.
René J.

--- On Thu, 5/7/09, Margaryan, Naira  wrote:

From: Margaryan, Naira 
Subject: [Histonet] FW: prover' pojaluista
To: "histonet@lists.utsouthwestern.edu" 
Date: Thursday, May 7, 2009, 11:23 AM

Dear Histonetters,

This is my question regarding IHC staining and water temperature: does IHC
staining depend on water temperature in washing step?

As always, I am using the same protocol for IHC. But one day it works
perfectly, another day, on the same tissue, it does not work. Everything is
same, solutions are fresh, and the only thing that is different is water
temperature. I am using running distilled water before and after Antigen
Retrieval step, after DAB, after counterstaining and after bluing reagent.
Dd-water goes into autostainer: all blocking steps and after primary, secondary
and tertiary. I am not using usual running water to exclude different Chlorine
concentrations in different days.

Have anybody of you ever paid attention to running distilled water temperature?
What water temperature (cold or warm) you will recommend for washing steps? If
this is critical, I can prepare my water up front.

Hope to get an answer soon.

Thanks,
Naira
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RE: [Histonet] Microtome Alignment

[Martin, Gary]

As an old carpenter I quickly adapted a "speed square" to do pretty much
the same thing as the $775.00 unit or the torpedo level.  The speed
square has the advantage of squaring the block holder to a true flat
surface (the base of the microtome) it works quite well ... it is
positive and quick.  See the Ace website below. I also use the torpedo
level as a double check. 
http://www.acehardware.com/search/index.jsp?kwCatId=&kw=speed%20square&o
rigkw=speed%20square&sr=1
 Gary  (in California) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor
Tobias
Sent: Thursday, May 07, 2009 9:21 AM
To: Jodie Robertson
Cc: histonet
Subject: Re: [Histonet] Microtome Alignment

I just went to Newcomer Supply to see what you get for $700, not much. 
Go to Ace Hardware and get each employee one of these. It will work just

as well and works on any microtome. Buy your staff lunch with what you
save.

http://www.acehardware.com/product/index.jsp?productId=1286367&cp=&fbc=1
&pg=3&kw=level&lmdn=Price+Range&fbn=StorePrice%7CUnder+%2425.00&fr=Store
Price%2FACE%2F%2F2500&parentPage=search&searchId=37640061873



Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use 
of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
disclose, reproduce, distribute, disseminate or otherwise use this 
transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.



Jodie Robertson wrote:
> Newcomer Supply.
>
>
> Jodie Robertson, HT (ASCP) QIHC
> Pathology Sciences Medical Group
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
kristen
> arvidson
> Sent: Thursday, May 07, 2009 6:24 AM
> To: histonet
> Subject: [Histonet] Microtome Alignment
>
> Does anyone know where I can an alignment device? 
>
>
>   
> ___
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>
> ___
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>   

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Re: [Histonet] NADPH-diaphorase

[Amy Porter]

Try Sigma-Aldrich.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  port...@msu.edu
Web:  www.humanpathology.msu.edu
- Original Message - 
From: "Breisch, Eric" 

To: 
Sent: Thursday, May 07, 2009 12:23 PM
Subject: [Histonet] NADPH-diaphorase


Hi, hoping that someone might be able to share with us where they
purchase NADPH-diaphorase for the NADPH stain. Thank you.



EAB











Eric A. Breisch, Ph.D.

Clinical Anatomist

Dept. of Pathology

Rady Children's Hospital and Health Center

Associate Clinical Professor of Anatomy

Dept. of Surgery

UCSD School of Medicine 




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Re: [Histonet] Microtome Alignment

[Victor Tobias]

I just went to Newcomer Supply to see what you get for $700, not much. 
Go to Ace Hardware and get each employee one of these. It will work just 
as well and works on any microtome. Buy your staff lunch with what you save.


http://www.acehardware.com/product/index.jsp?productId=1286367&cp=&fbc=1&pg=3&kw=level&lmdn=Price+Range&fbn=StorePrice%7CUnder+%2425.00&fr=StorePrice%2FACE%2F%2F2500&parentPage=search&searchId=37640061873 



Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use 
of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
disclose, reproduce, distribute, disseminate or otherwise use this 
transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.




Jodie Robertson wrote:

Newcomer Supply.


Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Thursday, May 07, 2009 6:24 AM
To: histonet
Subject: [Histonet] Microtome Alignment

Does anyone know where I can an alignment device? 



  
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[Histonet] NADPH-diaphorase

[Breisch, Eric]

Hi, hoping that someone might be able to share with us where they
purchase NADPH-diaphorase for the NADPH stain. Thank you.

 

EAB

 

 

 

 

 

Eric A. Breisch, Ph.D.

Clinical Anatomist

Dept. of Pathology

Rady Children's Hospital and Health Center

Associate Clinical Professor of Anatomy

Dept. of Surgery

UCSD School of Medicine 

 

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[Histonet] Remove from e-mail

[koswalt]

Please remove me from your e-mail lists.  Thank You, Kim



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[Histonet] how to unregister from histo-net

[koswalt]

Could anyone help me.  I am trying to un-listg with the histo-net  
services.  Please let me know.  Thanks, Kim




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RE: [Histonet] Microtome Alignment

[Jodie Robertson]

Newcomer Supply.


Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Thursday, May 07, 2009 6:24 AM
To: histonet
Subject: [Histonet] Microtome Alignment

Does anyone know where I can an alignment device? 


  
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[Histonet] FW: prover' pojaluista

[Margaryan, Naira]

Dear Histonetters,

This is my question regarding IHC staining and water temperature: does IHC 
staining depend on water temperature in washing step?

As always, I am using the same protocol for IHC. But one day it works 
perfectly, another day, on the same tissue, it does not work. Everything is 
same, solutions are fresh, and the only thing that is different is water 
temperature. I am using running distilled water before and after Antigen 
Retrieval step, after DAB, after counterstaining and after bluing reagent. 
Dd-water goes into autostainer: all blocking steps and after primary, secondary 
and tertiary. I am not using usual running water to exclude different Chlorine 
concentrations in different days.

Have anybody of you ever paid attention to running distilled water temperature? 
What water temperature (cold or warm) you will recommend for washing steps? If 
this is critical, I can prepare my water up front.

Hope to get an answer soon.

Thanks,
Naira
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RE: [Histonet] Microtome Alignment

[Bernice Frederick]

Microm sells a device called a histocollimeter, however, it only attaches to
their microtomes to my knowledge.
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Thursday, May 07, 2009 8:24 AM
To: histonet
Subject: [Histonet] Microtome Alignment

Does anyone know where I can an alignment device? 


  
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RE: [Histonet] Request for Staining Advice: Rabbit Calvaria & underlying Cerebral Hemishpere

[Shakun Aswani]

Try Goldner's Trichrome Stain. I have protocol if you want

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean
McBride
Sent: Thursday, May 07, 2009 8:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Request for Staining Advice: Rabbit Calvaria &
underlying Cerebral Hemishpere

Colleagues,

I have been charged with the task of evaluating an hydroxyapatite coated
biomaterial implanted in the calvaria of a rabbit model.

The calvaria and cerebral hemisphere were excised en bloc, and the
objective
of the experiment is to assess not only new bone formation and
osseointegration but also any pathological effects on the dura mater,
arachnoid, pia mater and underlying cerebral hemisphere.

I have assessed both brain and bone individually in the past, but never
en
bloc.  The specimens have been embedded in pmma and mounted for thick
ground
section (~30 - 35 microns) evaluation.  Does anyone have any suggestions
for
staining techniques that will show both bone & brain morphology in an en
bloc specimen?


Kind regards,


~Sean McBride


Senior Researcher
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-901-7540 (m)
412-268-8641 (fax)
smcbr...@cs.cmu.edu


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[Histonet] Request for Staining Advice: Rabbit Calvaria & underlying Cerebral Hemishpere

[Sean McBride]

Colleagues,

I have been charged with the task of evaluating an hydroxyapatite coated
biomaterial implanted in the calvaria of a rabbit model.

The calvaria and cerebral hemisphere were excised en bloc, and the objective
of the experiment is to assess not only new bone formation and
osseointegration but also any pathological effects on the dura mater,
arachnoid, pia mater and underlying cerebral hemisphere.

I have assessed both brain and bone individually in the past, but never en
bloc.  The specimens have been embedded in pmma and mounted for thick ground
section (~30 ­ 35 microns) evaluation.  Does anyone have any suggestions for
staining techniques that will show both bone & brain morphology in an en
bloc specimen?


Kind regards,


~Sean McBride


Senior Researcher
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-901-7540 (m)
412-268-8641 (fax)
smcbr...@cs.cmu.edu


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RE: [Histonet] New HTL Program!

[Kemlo Rogerson]

Congratulations from the UK. As you know we've had this in place for many years 
and resulted in the profession having a higher profile; I hope the rest will 
follow for you. 


 





Kemlo Rogerson  
e-mail kemloroger...@nhs.net if not at work.
DD   01934 647057 or extension 3311 Mob 07749 754194; 
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer
This e-mail is confidential and privileged. If you are not the intended 
recipient please accept my apologies; please do not disclose, copy or 
distribute information in this e-mail or take any action in reliance on its 
contents: to do so is strictly prohibited and may be unlawful. Please inform me 
that this message has gone astray before deleting it. Thank you for your 
co-operation

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: 07 May 2009 15:03
To: Sebree Linda A
Cc: histonet@lists.utsouthwestern.edu; Kimberly Feaster
Subject: Re: [Histonet] New HTL Program!



This is great news!!  Good luck and let's all wish this school success for many 
in years into the future.  It sounds like a very good program. 



Pam Marcum 

UPENN Vet School 

New Bolton Center 

- Original Message -
From: "Sebree Linda A" 
To: "Kimberly Feaster" , histonet@lists.utsouthwestern.edu
Sent: Thursday, May 7, 2009 9:57:03 AM GMT -05:00 US/Canada Eastern
Subject: RE: [Histonet] New HTL Program! 

This is great news for our field!  If I were 30 years younger. 

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave. 
Madison, WI 53792
(608)265-6596 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Feaster
Sent: Monday, May 04, 2009 8:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New HTL Program! 


West Virginia University School of Medicine Histotechnology Program is now 
accepting applications for fall 2009 enrollment! The WVU Histotechnology 
Program is offered as an area of emphasis within the Medical Laboratory Science 
major.  Students graduate with a Bachelor's of Science degree in Medical 
Laboratory Science.   The first two years consist of a pre-professional 
curriculum and the last two years consist of the Histotechnology Program 
curriculum.   The pre-professional curriculum can be completed at WVU main 
campus, one of the WVU regional campuses or any regionally accredited college 
or university. The Histotechnology Program curriculum is based at the WVU 
Robert C. 
Byrd Health Sciences Center.  The first year consists of a didactic schedule 
focusing on routine laboratory procedures with incorporated laboratory sessions 
and the second year will focus on complex procedures with on-site and clinical 
rotations.  Clinical rotations will be completed at the program's affiliated 
clinical laboratories located in West Virginia and Pennsylvania.   

If anyone would like more information or know someone who does, please
contact: 
Kimberly Feaster
Histotechnology Program Director
304-293-7628
kfeas...@hsc.wvu.edu 



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FW: [Histonet] Microtome Alignment

[Ian Montgomery]

What on the microtome do you want to align?

Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: 07 May 2009 14:24
To: histonet
Subject: [Histonet] Microtome Alignment

Does anyone know where I can an alignment device? 


  
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RE: [Histonet] New HTL Program!

[Shakun Aswani]

Fantastic!!! I was waiting for this happen.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly
Feaster
Sent: Monday, May 04, 2009 6:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New HTL Program!

West Virginia University School of Medicine Histotechnology Program is
now accepting applications for fall 2009 enrollment!
The WVU Histotechnology Program is offered as an area of emphasis
within the Medical Laboratory Science major.  Students graduate with a
Bachelor's of Science degree in Medical Laboratory Science.   The
first two years consist of a pre-professional curriculum and the last
two years consist of the Histotechnology Program curriculum.   The
pre-professional curriculum can be completed at WVU main campus, one of
the WVU regional campuses or any regionally accredited college or
university.  
The Histotechnology Program curriculum is based at the WVU Robert C.
Byrd Health Sciences Center.  The first year consists of a didactic
schedule focusing on routine laboratory procedures with incorporated
laboratory sessions and the second year will focus on complex procedures
with on-site and clinical rotations.  Clinical rotations will be
completed at the program's affiliated clinical laboratories located in
West Virginia and Pennsylvania.  

If anyone would like more information or know someone who does, please
contact:
Kimberly Feaster
Histotechnology Program Director
304-293-7628
kfeas...@hsc.wvu.edu 



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Re: [Histonet] New HTL Program!

[Pamela Marcum]

This is great news!!  Good luck and let's all wish this school success for many 
in years into the future.  It sounds like a very good program. 



Pam Marcum 

UPENN Vet School 

New Bolton Center 

- Original Message - 
From: "Sebree Linda A"  
To: "Kimberly Feaster" , 
histonet@lists.utsouthwestern.edu 
Sent: Thursday, May 7, 2009 9:57:03 AM GMT -05:00 US/Canada Eastern 
Subject: RE: [Histonet] New HTL Program! 

This is great news for our field!  If I were 30 years younger. 

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 



-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly 
Feaster 
Sent: Monday, May 04, 2009 8:56 AM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] New HTL Program! 


West Virginia University School of Medicine Histotechnology Program is 
now accepting applications for fall 2009 enrollment! The WVU 
Histotechnology Program is offered as an area of emphasis within the 
Medical Laboratory Science major.  Students graduate with a 
Bachelor's of Science degree in Medical Laboratory Science.   The 
first two years consist of a pre-professional curriculum and the last 
two years consist of the Histotechnology Program curriculum.   The 
pre-professional curriculum can be completed at WVU main campus, one of 
the WVU regional campuses or any regionally accredited college or 
university.   
The Histotechnology Program curriculum is based at the WVU Robert C. 
Byrd Health Sciences Center.  The first year consists of a didactic 
schedule focusing on routine laboratory procedures with incorporated 
laboratory sessions and the second year will focus on complex procedures 
with on-site and clinical rotations.  Clinical rotations will be 
completed at the program's affiliated clinical laboratories located in 
West Virginia and Pennsylvania.   

If anyone would like more information or know someone who does, please 
contact: 
Kimberly Feaster 
Histotechnology Program Director 
304-293-7628 
kfeas...@hsc.wvu.edu 



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RE: [Histonet] New HTL Program!

[Sebree Linda A]

This is great news for our field!  If I were 30 years younger.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly
Feaster
Sent: Monday, May 04, 2009 8:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New HTL Program!


West Virginia University School of Medicine Histotechnology Program is
now accepting applications for fall 2009 enrollment! The WVU
Histotechnology Program is offered as an area of emphasis within the
Medical Laboratory Science major.  Students graduate with a
Bachelor's of Science degree in Medical Laboratory Science.   The
first two years consist of a pre-professional curriculum and the last
two years consist of the Histotechnology Program curriculum.   The
pre-professional curriculum can be completed at WVU main campus, one of
the WVU regional campuses or any regionally accredited college or
university.  
The Histotechnology Program curriculum is based at the WVU Robert C.
Byrd Health Sciences Center.  The first year consists of a didactic
schedule focusing on routine laboratory procedures with incorporated
laboratory sessions and the second year will focus on complex procedures
with on-site and clinical rotations.  Clinical rotations will be
completed at the program's affiliated clinical laboratories located in
West Virginia and Pennsylvania.  

If anyone would like more information or know someone who does, please
contact:
Kimberly Feaster
Histotechnology Program Director
304-293-7628
kfeas...@hsc.wvu.edu 



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[Histonet] What are peoples feelings on pre labling slides?

[Leah Nichols]

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[Histonet] What are peoples feelings on pre labling slides?

[Leah Nichols]

   An  independent  QA  study was completed in our AP department.  One of
   the  report's  recommendations was that we do not pre-label our slides
   and  develop  a  "just  in  time" slide labelling process.  We are now
   looking into this and would appreciate your feedback/comments on "just
   in time" slide labelling.
   The  questions  I  have for labs that has developed their own "just in
   time" slide labelling process are:
 * Who labels your slides?
 * When do you label your slides?
 * What is your process?
 * Has this decreased  your error rate?
 * Off topic - when do you label your cassettes?

   Thanks,
   Leah Nichols
   Lean Facilitator
   Calgary Laboratory Services
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[Histonet] Cryo question

[Jennifer S Finke]

   To whom it may concern:

   I  have  a  question  about OTC emb= edded tissues. They were embedded
   sometime  ago  and  kept  at  -20C.  Is  there  a=  way  to  re-embed,
   and re-hydrate the tissue?
   Thank you,

   Jennifer Finke-Dwyer
   Department of Pharmacology and Experi= mental Neuroscience
   University of Nebraska Medical Center
   985880 Nebr= aska Medical Center
   Omaha, NE 68198-5880
   Phone: (402)559-8925
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[Histonet] New HTL Program!

[Kimberly Feaster]

West Virginia University School of Medicine Histotechnology Program is
now accepting applications for fall 2009 enrollment!
The WVU Histotechnology Program is offered as an area of emphasis
within the Medical Laboratory Science major.  Students graduate with a
Bachelor’s of Science degree in Medical Laboratory Science.   The
first two years consist of a pre-professional curriculum and the last
two years consist of the Histotechnology Program curriculum.   The
pre-professional curriculum can be completed at WVU main campus, one of
the WVU regional campuses or any regionally accredited college or
university.  
The Histotechnology Program curriculum is based at the WVU Robert C.
Byrd Health Sciences Center.  The first year consists of a didactic
schedule focusing on routine laboratory procedures with incorporated
laboratory sessions and the second year will focus on complex procedures
with on-site and clinical rotations.  Clinical rotations will be
completed at the program’s affiliated clinical laboratories located in
West Virginia and Pennsylvania.  

If anyone would like more information or know someone who does, please
contact:
Kimberly Feaster
Histotechnology Program Director
304-293-7628
kfeas...@hsc.wvu.edu 



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Re: [Histonet] Microtome Alignment

[Akemi Allison-Tacha]

David Davis from CO designed an alignment device and can be purchased from 
Newcomer supply or I think his e-mail is still the 
following: davidda...@uchsc.edu
Good Luck,
Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-ta...@apmglab.com
P: E-Mail: akemiat3...@yahoo.com



--- On Thu, 5/7/09, kristen arvidson  wrote:

From: kristen arvidson 
Subject: [Histonet] Microtome Alignment
To: "histonet" 
Date: Thursday, May 7, 2009, 6:24 AM

Does anyone know where I can an alignment device? 


      
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RE: [Histonet] Microtome Alignment

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

Newcomer sells them. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Thursday, May 07, 2009 9:24 AM
To: histonet
Subject: [Histonet] Microtome Alignment

Does anyone know where I can an alignment device? 


  
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[Histonet] Microtome Alignment

[kristen arvidson]

Does anyone know where I can an alignment device? 


  
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Re: [Histonet] wright-giemsa stain on tissue

[Rene J Buesa]

Medea:
Under separate cover I am sending an article I wrote on Giemsa for FFPE tissues.
René J.

--- On Wed, 5/6/09, Medea McGraw  wrote:

From: Medea McGraw 
Subject: [Histonet] wright-giemsa stain on tissue
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 6, 2009, 5:24 PM

Renee,
 I was just looking in the histonet archives concerning doing a Wright
Giemsa stain on FFPE tissue. We have a a FFPE cell pellet and was wondering
the protocol on how you would stain this tissue? In the past, I have
performed Wright Giemsa stains only on BAL¹s / cytospins and therefore I am
unsure of how to properly stain the researchers tissue. Any idea? Input on
how to go about doing this? What if you were to rehydrate down to water and
perform the test from there? Any suggestions would be greatly appreciated.

Regards,

Medea
-- 
Medea J. McGraw BS, HT, HTL(ASCP) CM
IHC Research Histotechnologist
Phenopath Laboratories
Phone: 206-374-9000 x1033
Fax: 206-374-9009



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RE: [Histonet] Gram & AFB staining FNA smears

[Kemlo Rogerson]

Agree; I guess the difference is that FNAC and Micro samples haven't been 
subjected to processing like the tissue block sections. The tissue stains have 
been shown to work on something that has been fixed, dehydrated, set in hot 
wax, cut, rehydrated and then stained. The FNAC and Micro samples may or may 
not have been fixed (although I concede air drying is a form of fixation) and 
the stains used on them have been shown to work. 

The logic that these techniques are interchangeable is not only flawed but 
(oxy)moronic.





Kemlo Rogerson  
e-mail kemloroger...@nhs.net if not at work.
DD   01934 647057 or extension 3311 Mob 07749 754194; 
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer
This e-mail is confidential and privileged. If you are not the intended 
recipient please accept my apologies; please do not disclose, copy or 
distribute information in this e-mail or take any action in reliance on its 
contents: to do so is strictly prohibited and may be unlawful. Please inform me 
that this message has gone astray before deleting it. Thank you for your 
co-operation

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 06 May 2009 17:24
To: histonet@lists.utsouthwestern.edu; Angela Bitting
Subject: Re: [Histonet] Gram & AFB staining FNA smears

The "doc" is wrong, otherwise your histology sections to be stained with Gram & 
AFB should also be sent to micro. Perhaps you will not have to do them anymore. 
Your "doc's" reasoning is purely oxymoronic.
René J.

--- On Wed, 5/6/09, Angela Bitting  wrote:

From: Angela Bitting 
Subject: [Histonet] Gram & AFB staining FNA smears
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 6, 2009, 9:22 AM

The subject of staining FNA smears with AFB and Gram stains in Histology vs in 
Micro came up today. Is there a reason that FNAs can't be stained the same way 
we stain our tissue sections? One of our docs was under the impression that 
it's not acceptable to use the staining methods we use in our Histology lab.
I don't know what method Micro labs use, so I was hoping someone could shed 
some light on this subject for me.

Thanks, as always,
Angie

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email However many electrons were 
severly inconvienienced!




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