[Histonet] Re: vacant post

2009-05-13 Thread Anne van Binsbergen
Dear ALL

My signature (see the very bottom of my email) has my location !!

ABU DHABI is the capital of the United Arab Emirates - about 160kms from
Dubai
I am not permitted to discuss salaries but the current monthly salary of a
Senior Medical Technologist in this hospital is around 11 500 AED (thats the
currency of the UAE) - which is probably somewhere in the region of $3000 a
month

Remember that I need current proven IHC skills as well as general all-round
Histo lab experience
Your qualifications should be at least a 3 year Diploma level with a valid
licence to practice in Histology
 Please send me your CVs asap and I will pass them on to our Recruiment
department - include your contact details with an email address
cheers
Anne


2009/5/13 Anne van Binsbergen 

> Hello Histonetters
> My Immuno tech has left and I urgently need a replacement
> Its a full-time position and requires good IHC experience as well as
> routine Histo experience - an all-rounder
> Need someone with at least 7-10 years experience as its a Senior post
> Anyone interested?
> Send me your CVs
> --


-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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[Histonet] RE: Histonet Digest, Vol 66, Issue 15

2009-05-13 Thread Joanne Clark

Sprick, Stegall & Associates from Salem OH.  Their website is
www.sprickstegall.com.  I attended a conference where they did a one day
workshop on how to set up LEAN and they seemed very experienced.

Joanne Clark HT
Histology Supervisor
Pathology Consultants of New Mexico
--

Message: 3
Date: Tue, 12 May 2009 15:15:39 -0400
From: "Anita Thorn" 
Subject: [Histonet] Lean Consultants
To: 
Message-ID:
<602863d272b56749a70cba315d7dc70203b32...@bmhexch.bmhvt.org>
Content-Type: text/plain;   charset="us-ascii"

Are there any companies out there that go around helping set up LEAN
Histology laboratories?  If anybody can supply some company names I'd
appreciate it.


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[Histonet] oxytocin receptor IHC fixation and storage

2009-05-13 Thread Jan Shivers
I'm sending this for a researcher here.  He's got a lot of tissue in storage 
that's still 'wet' (still in various fixatives and bagged up).  The tissues 
have been in fixative for over 10 years.  He wants to know if he can still hang 
onto the tissue for future IHC use, or has it been in fixative too long for the 
antigen in question to be retrieved successfully.  (I don't need a protocol for 
oxytocin receptor IHC as of yet; I just need an answer to his storage question 
- because he's moving his materials from one lab space to another.)

I've never done oxytocin receptor IHC, so I don't know how difficult it is to 
retrieve it on the best of days.  Is there anyone out there who might know the 
answer to this question?

Thanks in advance.
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu
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Re: [Histonet] Toulidine blue stain

2009-05-13 Thread Geoff McAuliffe
I have always liked a very simple method from an older edition of 
Humanson's book.


1. Bring paraffin sections to water.
2. Stain in 0.1% Toluidine Blue in distilled water, 2-5 minutes
3. Rinse thoroughly in distilled water.
4. Dehydrate in 2 changes of acetone, 5 minutes each. Dehydrating in 
alcohol will wash all of the stain out.
5. Clear in xylene as usual. Use fresh xylene, not solutions containing 
carry-over alcohol from other procedures.


The second advantage of acetone dehydration is that metachromasia of 
cartilage, mast cells of some species, etc. is preserved.


Geoff

Jennifer Campbell wrote:

Hi everyone,
 
I'm trying to find a protocol for a toulidine blue stain for paraffin

sections.  I've looked in different text books and online and can't seem
to find anything helpful.  Does anyone have a good protocol for this?  I
need to order the stain rather soon but, first I want to know how much
I'm going to need to run the stain.
 
Thanks in advance,
 
Jen C.

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--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcaul...@umdnj.edu

**



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RE: [Histonet] Leica cryostat

2009-05-13 Thread Martin, Gary
We have an 1850 and like very much, but see if you can find one with the
ultra violet light for sterilization.  It would save a lot of hassle.
Gary 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dertien,
Janet
Sent: Wednesday, May 13, 2009 1:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica cryostat

Hello everyone,

 

We have decided to purchase a refurbished Leica cryostat and are trying
to decide which model to choose; does anyone have experience with the
1510, the CM 1800 or the 1800? Any feedback would be appreciated.
Thanks!

 

Janet

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[Histonet] Leica cryostat

2009-05-13 Thread Dertien, Janet
Hello everyone,

 

We have decided to purchase a refurbished Leica cryostat and are trying
to decide which model to choose; does anyone have experience with the
1510, the CM 1800 or the 1800? Any feedback would be appreciated.
Thanks!

 

Janet

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[Histonet] 2009 LSH Symposium/Convention-Additional Information

2009-05-13 Thread Montina Van Meter
 

Fellow Histonetters,

 

 The Louisiana Society for Histotechnology is pleased to announce
the 26th Annual Symposium/Convention: 

 

  "Your
Histeaux Surplus Package"

 

 
June 12 & 13, 2009

 

 
at the

 

 
Bourbon Orleans Hotel

 

 
717 Orleans St.

  

 
New Orleans, LA  70117

 

 
www.bourbonorleans.com

 

 

 

 

  The LSH room rates will be honored as long as rooms are available.
For those attendees who may want to visit the beautiful sights and
sounds of New Orleans, the Bourbon Orleans Hotel will honor the room
rates for three days prior and three days after the meeting.  Special
on-site parking rates for LSH attendees are available as well as public
parking around Jackson Square.  For reservations call 1-504-523- or
1-866-513-9744 and mention you are with the LSH group.  Do to relocation
of many of our members we would ask everyone who would like to receive a
brochure/membership form in the mail, email or fax, to please contact
Tina Van Meter at 225-603-0953, vanme...@pbrc.edu or Dixie Benoit at
337-233-1951, dixiehistoch...@bellsouth.net.  

 

 

 

Walk-ins are always welcome!  If you are planning on registering the
day of the meeting we would appreciate it if you would send us an email
or call Dixie or Tina prior to the meeting which will allow the LSH to
make arrangements for workshop handouts and food reservations.
Pre-registrants will automatically receive a complimentary buffet lunch
each day!  Please make additional copies of your registration form for
co-workers in your lab or facility that might be interested in
attending.  The LSH would also like to extend the invitation to our
fellow technologists and pathologists from surrounding states.  We
encourage everyone to attend in order to build our networking potential,
earn those valuable CEU's and enjoy the beautiful city of New Orleans.  

 

   There are many local attractions going on during the week of our
symposium such as: The Cajun - Zydeco Festival, Seafood Festival and the
Creole Tomato Festival all in the French Quarter/Jackson Square area.
Free admission to the festivals.   Check out the links below:


 

 
http://www.neworleansonline.com/news/2009/May/vieuxtodo.html

 

 
http://ww.neworleansonline.com/neworleans/tours/

 

 

 

2009 LSH State Meeting Workshops:

 

WS#1 - Am I Really Ready for this CAP Inspection?

 

WS#2 - Mouse to Horse: It's Not Human

 

WS#3 - Breast Cancer and the Standardization of HER2/neu Testing

 

WS#4 - Contemporary Trends in Immunohistochemistry 

 

WS#5 - Troubleshooting Routine Special Stains 

 

WS#6 - Which Code Do I Use?  (CPT Coding)

 

WS#7 - Are You REALLY Ready for the Next Catastrophic Event That Will
Affect Your Hospital or City?

 

WS#8 - 2 Crazy Cat Ladies Give Their Opinions of Life, Liberty, and Lab
Management

 

 We have a variety of topics presented by experienced speakers that
promises to benefit everyone.  The attendees will have access to several
scientific vendor exhibits during the entire symposium.  The LSH would
love to have y'all come on down to the Bourbon Orleans Hotel in the
French Quarter!

 

 

 

Hope to see you in June!

 

The LSH Committee

 

 

 

 

 

 

 

 

 

 

 

 

Montina J. Van Meter, HT (ASCP)

Lab Manager

Autonomic Neuroscience

Pennington Biomedical Research Center

6400 Perkins Rd.

Baton Rouge, LA  70791

225-763-2564

 

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[Histonet] mouse CD115

2009-05-13 Thread Andrea Hooper
Anyone working with CD115 in mouse? If so, may I contact you as I 
have a few questions?


Andrea Hooper
anh2006 @ med.cornell.edu
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[Histonet] mouse CD11c

2009-05-13 Thread Andrea Hooper
Is anyone working on CD11c in murine tissues? If so, can we exchange 
emails? ... I have some questions.


Andrea Hooper
anh2...@med.cornell.edu
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[Histonet] Looking for part-time Histology work in Phoenix AZ

2009-05-13 Thread Jill Cox
Hi All,
I am looking for part-time Histology work in Phoenix AZ. I am available for 
almost any shift, my current employer is very flexible with my hours. I have 
been in field for 17 years and know all aspects of Histology. Please respond to 
this email if you have any questions.
Thanks!!!





Jill Cox HT (ASCP) 
 
 
 

 
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[Histonet] vacant post

2009-05-13 Thread Anne van Binsbergen
Hello Histonetters
My Immuno lead tech has left and I urgently need a replacement
Its a full-time position and requires good IHC experience as well as routine
Histo experience - an all-rounder
Need someone with at least 7-10 years experience as its a Senior post
Anyone interested?
Send me your CVs
-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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RE: [Histonet] Question about DAB waste

2009-05-13 Thread Smith, Allen
We were told that, in Miami-Dade County, we would have to apply for a license 
to do this.  We gave up on the cumbersome licensing procedure.
Allen A. Smith, Ph.D.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich
Sent: Wednesday, May 13, 2009 12:10 PM
To: histonet@lists.utsouthwestern.edu; jcampb...@vdxpathology.com
Subject: Re: [Histonet] Question about DAB waste

We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE
HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184.

1. Prepare the following aqeous stock solutions:
a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter)
b.  2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter)
2. Dilute DAB solution of necessary so that its concentration does not
exceed 0.9 mg/ml.
3. For each 10ml of DAB solution, add:
a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter)
b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%)
4. Allow mixture to stand for at least 10 hours.  It is now
non-mutagenic.
5. Decolorize the mixture with ascorbic acid (add powder until color
disappears).  This too is an oxidation/reduction reaction.
6. Neutralize the decolorized mixture with sodium bicarbonate (test with
pH meter, pH paper or dipsticks).  Note:  we recommend using magnesium
hydroxide/oxide instead of sodium bicarbonate.
7. Discard the solution down the drain provided that local wastewater
authorities have given their approval.

Hope this helps,
Lynette

>>> "Jennifer Campbell"  05/13/09 8:51 AM
>>>
We just purchased a Dako Autostainer and are in the process of figuring
out how to get rid of the haz waste that we will be generating.  The
machine separates the haz from non-haz, thankfully.  I've been
communicating with our waste facility and have been having some
difficulty.  They want to know a lot of specifics like, how much waste
we will be brining to them at a time and how often, what sort of
containers we will be bringing it in etc.  Do you let the haz waste
carboy get to a certain level before emptying it?  Is it ok to pour this
waste into plastic containers of some sort (we were going to use empty
distilled water bottles) to transport them to the facility?  
I would appreciate any insight.  Thank you.
 
Jennifer C.
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[Histonet] Question about DAB Waste

2009-05-13 Thread Eric Sulkosky
Have any of you tried the Dab/Out? It is a system that extracts DAB from
liquid waste. The liquid passes through a cartridge and captures the DAB,
then you can dispose cartridge as a dry solid waste and pour the rest of the
liquid down the drain. It is known to be cost effective and environmentally
safe. Just an alternative to think of.

Hope this helps
Eric
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Re: [Histonet] Question about DAB waste

2009-05-13 Thread Lynette Pavelich
We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE
HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184.

1. Prepare the following aqeous stock solutions:
a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter)
b.  2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter)
2. Dilute DAB solution of necessary so that its concentration does not
exceed 0.9 mg/ml.
3. For each 10ml of DAB solution, add:
a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter)
b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%)
4. Allow mixture to stand for at least 10 hours.  It is now
non-mutagenic.
5. Decolorize the mixture with ascorbic acid (add powder until color
disappears).  This too is an oxidation/reduction reaction.
6. Neutralize the decolorized mixture with sodium bicarbonate (test with
pH meter, pH paper or dipsticks).  Note:  we recommend using magnesium
hydroxide/oxide instead of sodium bicarbonate.
7. Discard the solution down the drain provided that local wastewater
authorities have given their approval.

Hope this helps,
Lynette

>>> "Jennifer Campbell"  05/13/09 8:51 AM
>>>
We just purchased a Dako Autostainer and are in the process of figuring
out how to get rid of the haz waste that we will be generating.  The
machine separates the haz from non-haz, thankfully.  I've been
communicating with our waste facility and have been having some
difficulty.  They want to know a lot of specifics like, how much waste
we will be brining to them at a time and how often, what sort of
containers we will be bringing it in etc.  Do you let the haz waste
carboy get to a certain level before emptying it?  Is it ok to pour this
waste into plastic containers of some sort (we were going to use empty
distilled water bottles) to transport them to the facility?  
I would appreciate any insight.  Thank you.
 
Jennifer C.
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[Histonet] peroxidase block

2009-05-13 Thread njoydobro
good morning,

 Is there anyone out there who has eliminated using Peroxidase Block on 
either IHC or ICC?

Thanks,
Gene
Cleveland Clinic
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[Histonet] 0.2M borate acid pH 7.0

2009-05-13 Thread TF
My recipe for 0.2M borate acid is:

Sodium Borate acid.10 H2O (molecular weight 381.42, Na4B4O4.10H2O)  
19.07 gram
H2O 
1 Litre

Adjust pH = 7.0 with 5M HCl

Is this fine?

2009-05-11 



TF 
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Re: Re: [Histonet] xylene substitues

2009-05-13 Thread TF
tulane !!!


2009-05-13 



TF 



发件人: Lynette Pavelich 
发送时间: 2009-05-13  23:49:59 
收件人: njoydo...@aol.com; Histonet@lists.utsouthwestern.edu 
抄送: 
主题: Re: [Histonet] xylene substitues 
 
We use Propar by Anatech. It works great.
Lynette
>>>  05/13/09 10:25 AM >>>
just wanting to see what everyone's favorite xylene substitute is for
clearing slides before coverslipping?? 
Gene
Cleveland Clinic
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Re: [Histonet] xylene substitues

2009-05-13 Thread Lynette Pavelich
We use Propar by Anatech. It works great.
Lynette

>>>  05/13/09 10:25 AM >>>
just wanting to see what everyone's favorite xylene substitute is for
clearing slides before coverslipping?? 

Gene
Cleveland Clinic
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[Histonet] blood spots

2009-05-13 Thread Helen E Johnson

Does anyone have protocols for doing histology and hematology on blood
spots?

Helen Johnson  (he...@health.state.ny.us)



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RE: [Histonet] xylene substitutes

2009-05-13 Thread Blazek, Linda
I second Gary's comments.  We use Formula 83 in all phases also.

Linda 

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary
Sent: Wednesday, May 13, 2009 10:50 AM
To: njoydo...@aol.com; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] xylene substitutes

We use Formula 83 ... we have had no problems in all phases.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
njoydo...@aol.com
Sent: Wednesday, May 13, 2009 7:25 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] xylene substitues

just wanting to see what everyone's favorite xylene substitute is for
clearing slides before coverslipping?? 

Gene
Cleveland Clinic
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Re: [Histonet] xylene substitues

2009-05-13 Thread V. Neubert

N-butyl acetate aka butyl ethanoate.

njoydo...@aol.com wrote:
just wanting to see what everyone's favorite xylene substitute is for clearing slides before coverslipping?? 


Gene
Cleveland Clinic
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RE: [Histonet] xylene substitutes

2009-05-13 Thread Martin, Gary
We use Formula 83 ... we have had no problems in all phases.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
njoydo...@aol.com
Sent: Wednesday, May 13, 2009 7:25 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] xylene substitues

just wanting to see what everyone's favorite xylene substitute is for
clearing slides before coverslipping?? 

Gene
Cleveland Clinic
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[Histonet] xylene substitues

2009-05-13 Thread njoydobro
just wanting to see what everyone's favorite xylene substitute is for clearing 
slides before coverslipping?? 

Gene
Cleveland Clinic
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[Histonet] IHC - PGDFR reactive in Canines

2009-05-13 Thread Sandra Cheasty
Hello,

Does anyone have experience using PGDFR in canines and if so, is 
there a canine species control that is known to react with PGDFR?

Thank you,

Sandy



Sandra Cheasty

Histology Supervisor

UW-Madison

School of Veterinary Medicine

608 263-1680

















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Re: [Histonet] Job Openings

2009-05-13 Thread Angela Bitting
We have a part-time dayshift and a full-time night shift opening for a HT. If 
you're interested here's our web address.
www.geisinger.org 

or call me directly.

Angie



Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


>>>  5/12/2009 1:15 PM >>>
Good Afternoon-

How do I review the job openings on the Histonet site?







Eric Halpern
Sr.Search Consultant, Lab Sciences and Imaging Division
Permanent Placement Division
CompHealth Associates, Inc.
6451 North Federal Highway, Suite 702
Fort Lauderdale, Florida 33308
Phone: 866-782-9029, ext. 5852
Cell  954-336-8913
Fax: 800-420-2329
email: eric.halp...@comphealth.com 
www.comphealth.com 



Please note: "This is a commercial email from CompHealth Associates, Inc. 
If  you do not want to receive future emails from CompHealth Associates, 
Inc. , please reply to the sender of this email and ask to be removed from 
our list."



Customer service is the key to success.
Are you satisfied with your experience? Send your comments to my manager 
at 
valerie.patter...@comphealth.com.
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RE: [Histonet] RE: Question about DAB waste

2009-05-13 Thread Weems, Joyce
We had ours analyzed by our Hazardous Waste Disposal Co. They provide
labels for the drums. We have a drum for the waste from the special
stains, the DAB waste, and the xylene waste from the recycler - labels
all provided by the disposal company. j

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Wednesday, May 13, 2009 9:12 AM
To: Smith, Allen; Jennifer Campbell
Cc: Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Question about DAB waste

On this subject at our recent CAP inspection a question came up about
the labeling of the waste containers that held the DAB waste from our
Ventana stainer.  We had the container labeled as "Hazardous Chemical
Waste".  We also keep it in a large plastic carboy which our maintenance
dept. then empties into a large metal can that is picked up by a
hazardous waste disposal company as the cans become full.  The inspector
insisted that the waste container itself from our Ventana stainer should
have listed every chemical that could possibly be in the waste.
How are the DAB waste containers labeled at other facilities?
Thank you,
Carol 
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RE: [Histonet] RE: Question about DAB waste

2009-05-13 Thread Carol Bryant
On this subject at our recent CAP inspection a question came up about
the labeling of the waste containers that held the DAB waste from our
Ventana stainer.  We had the container labeled as "Hazardous Chemical
Waste".  We also keep it in a large plastic carboy which our maintenance
dept. then empties into a large metal can that is picked up by a
hazardous waste disposal company as the cans become full.  The inspector
insisted that the waste container itself from our Ventana stainer should
have listed every chemical that could possibly be in the waste.
How are the DAB waste containers labeled at other facilities?
Thank you, 
Carol 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith,
Allen
Sent: Wednesday, May 13, 2009 8:57 AM
To: 'Jennifer Campbell'
Cc: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Question about DAB waste

We keep our DAB waste in a plastic container and give it to our waste
disposal company every 6 months.
No one has objected to this procedure.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, FL

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Campbell
Sent: Wednesday, May 13, 2009 8:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about DAB waste

We just purchased a Dako Autostainer and are in the process of figuring
out how to get rid of the haz waste that we will be generating.  The
machine separates the haz from non-haz, thankfully.  I've been
communicating with our waste facility and have been having some
difficulty.  They want to know a lot of specifics like, how much waste
we will be brining to them at a time and how often, what sort of
containers we will be bringing it in etc.  Do you let the haz waste
carboy get to a certain level before emptying it?  Is it ok to pour this
waste into plastic containers of some sort (we were going to use empty
distilled water bottles) to transport them to the facility?  
I would appreciate any insight.  Thank you.
 
Jennifer C.
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Re: [Histonet] Lean Consultants

2009-05-13 Thread Angela Bitting
Here's the website for consulting group we used: www.sprickstegall.com 

Angie

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


>>> "Anita Thorn"  5/12/2009 3:15 PM >>>
Are there any companies out there that go around helping set up LEAN
Histology laboratories?  If anybody can supply some company names I'd
appreciate it.


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[Histonet] RE: Question about DAB waste

2009-05-13 Thread Smith, Allen
We keep our DAB waste in a plastic container and give it to our waste disposal 
company every 6 months.
No one has objected to this procedure.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, FL

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
Campbell
Sent: Wednesday, May 13, 2009 8:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about DAB waste

We just purchased a Dako Autostainer and are in the process of figuring
out how to get rid of the haz waste that we will be generating.  The
machine separates the haz from non-haz, thankfully.  I've been
communicating with our waste facility and have been having some
difficulty.  They want to know a lot of specifics like, how much waste
we will be brining to them at a time and how often, what sort of
containers we will be bringing it in etc.  Do you let the haz waste
carboy get to a certain level before emptying it?  Is it ok to pour this
waste into plastic containers of some sort (we were going to use empty
distilled water bottles) to transport them to the facility?  
I would appreciate any insight.  Thank you.
 
Jennifer C.
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[Histonet] Question about DAB waste

2009-05-13 Thread Jennifer Campbell
We just purchased a Dako Autostainer and are in the process of figuring
out how to get rid of the haz waste that we will be generating.  The
machine separates the haz from non-haz, thankfully.  I've been
communicating with our waste facility and have been having some
difficulty.  They want to know a lot of specifics like, how much waste
we will be brining to them at a time and how often, what sort of
containers we will be bringing it in etc.  Do you let the haz waste
carboy get to a certain level before emptying it?  Is it ok to pour this
waste into plastic containers of some sort (we were going to use empty
distilled water bottles) to transport them to the facility?  
I would appreciate any insight.  Thank you.
 
Jennifer C.
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