[Histonet] NSH Meeting Hotels
Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium. I went to the NSH website and looked under the "Hotel & Travel" icon and saw that the only hotel that wasn't Sold Out was the Double Tree. I called and they never heard of us "NSH". I read all the information to the reservation person and she came up with a blank. I requested the supervisor. After searching for about 15 minutes, she found us under histotechnology. Anyone trying to book a room should state that our GROUP CODE is "CI0N" Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Negative Staining Positive
Has anyone noticed their negative mouse having some positive staining using the Ventana Benchmark XT? We use the iview detection. Any ideas? Thank you. -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opportunity for technologist interested in development of educational materials
This is a great opportunity for someone with expertise in laboratory medicine and in education. MediaLabInc. (http://www.medialabinc.net), located in metro Atlanta, GA, is a leading provider of online compliance and training software for the clinical laboratory industry. We presently deliver over 300,000 online course events to individuals and institutions annually, and our customer base continues to expand, as does the depth, breath, and quality of our course offerings. We are seeking an additional medical technologist (MT) to join our team. Experience as a classroom or online educator and experience in writing technical or educational materials is essential. Sub-speciality training, experience in laboratory administration, safety, or compliance would be helpful. Knowledge or experience in instructional design, digital photography, photomicroscopy, and video production will also be considered. We realize that no one individual will have all these skills, but a strong background as a clinical laboratorian and educator is essential, and all interested MT applicants with at least 5 years of laboratory and/or educational experience are strongly encouraged to apply. The successful candidate will work closely with our team to help us to develop and update courseware that continues to meet the ever changing needs of clinical laboratories. He or she will also work with other contributing course authors and local laboratory managers and technologists. Pay and benefits are negotiable and highly competitive. Work from home several days per week, and enjoy working in our comfortable office in suburban Atlanta the rest of the time. Some opportunity for travel is available. Preference will be given to residents of metro Atlanta, but we will consider an outstanding candidate from the United States or Canada who is willing to relocate. We will also consider part-time employment for the right candidate who wants to work from his / her home location in the U.S. or Canada. Please send your CV to Paul Fekete, MD, via email attachment to fek...@gmail.com. For more information about MediaLab, visit http://www.medialabinc.net. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laura Miller is on vacation.
I will be out of the office starting 08/13/2009 and will not return until 08/17/2009. I will respond to your email on Monday. Thanks! __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Specimen Storage
We used to store them directly in the sealed Kapak pouches. I believe there are some "for home" commercial alternatives, although probably for frozen storage. Worth exploring "out of the lab" alternatives. René J. --- On Thu, 8/13/09, Susan Raibley wrote: From: Susan Raibley Subject: [Histonet] Specimen Storage To: histonet@lists.utsouthwestern.edu Date: Thursday, August 13, 2009, 3:18 PM I work for a research company and am looking at ideas for storage of specimens. We currently use Bitran bags to keep the extra parts with some formalin and then heat seal them in the Kapak heat seal bags. The Bitran bags are getting so expensive and tend to leak. Does anyone have a better system for long term storage of multiple animal specimens? Thanks! Susan Bincsik, HT (ASCP) __ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] X-gal staining
Hello, I was wondering whether anyone might advise me on X-gal staining. I am looking at the expression of LacZ knocked into the locus of our gene of interest. We cryoembed, take sections and stain for beta-galactosidase activity using X-gal. The blue stain is often punctuate and noticeably limited to the nucleus, whereas in other areas the staining is diffuse and appears throughout the cytoplasm of the cells. I have guessed that this may be related to the level of expression; i.e. that in cells with a low reporter activity beta-gal is limited to the nucleus, but that at higher expression the whole cell becomes riddled with it. If anyone knows anything about the subcellular distribution/localisation of beta-gal in mammalian cells, or can direct me to some good literature I would be grateful. Best wishes Nick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Specimen Storage
I work for a research company and am looking at ideas for storage of specimens. We currently use Bitran bags to keep the extra parts with some formalin and then heat seal them in the Kapak heat seal bags. The Bitran bags are getting so expensive and tend to leak. Does anyone have a better system for long term storage of multiple animal specimens? Thanks! Susan Bincsik, HT (ASCP) __ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Freezing in Hexane
Dorothy Murphy Traczyk at Murphy-Traczyk & Associates in Point Pleasant, NJ asks about using hexane for snap-freezing. I wouldn't want any of the hexanes (there are several isomers) or 2-methylbutane around my lab because of the explosion hazard if I could avoid it. Look up 3M Novec Engineered Fluid HFE-7100 (methyl nonafluoroisobutyl ether) in the Histonet archives (accessible through Google). Probably usable for this purpose, and not flammable. If you do this, please report your experience to Histonet - several of us on here are interested. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Picric Acid
Hello everyone! I would like to know what all of you are doing now that picric acid is so hard to get? We use it for bouin's, trichrome and gram stain. I know we can buy these already made up, but we wanted to know if there might be other alternatives. I'll be off tomorrow (state fair time), but will check my e-mail first thing monday morning. Thank-you in advance for all your help! Diane ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] advice about bone section please
Hi all, I'm planning to do paraffin embed section on mouse joint (E12~P10) to detect the GFP-tagged protein expression. Since bone has autofluorescence, I'm wondering whether IHC would be a good choice for postnatal bone, and can I do immunofluorescence on joint in E12~16 mouse without having autofluorescence effect? I've tried cryosection (10 micrometer) but the bone detached and doesn't give me good morphology so I decided to try paraffin. thank you all in advance. W ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] optimal IHC thickness
I always used 3-4µm and prefer HIER over enzymes. René J. --- On Thu, 8/13/09, Katelin Lester wrote: From: Katelin Lester Subject: [Histonet] optimal IHC thickness To: histonet@lists.utsouthwestern.edu Date: Thursday, August 13, 2009, 10:24 AM Hi everyone, I am staining lymph nodes for staging of breast cancer with CKAE1/AE3 and would like to know what everyone is cutting this type of slide at. I searched the archives and my understanding is that, for IHC, how thick the sections are cut is not necessarily critical. I typically cut all IHC at 4μm and I want to achieve the best results possible. Also, on a side note, the Data Sheet suggests using Trypsin, but I have been using Pepsin. Any comments or suggestions about time, quality of Trypsin vs. Pepsin? Thank you, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Freezing in Hexane
Hi Dorothy, I've not used hexane. I think 2-methly butane is more commonly used if you are, as I suspect, snap freezing in a solvent chilled in liquid nitrogen. The solvent just needs to have a low freezing point. 2-methyl butane freezes at about - 150 degrees C. I do not know if hexane has a lower freezing point but you can determine that with an internet search. My point in all of this is that you may have other choices for snap freezing if you can't get an answer to your original question re hexane as long as you are able to freeze the needed temperature. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tracz...@aol.com Sent: Wednesday, August 12, 2009 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing in Hexane I have a procedure that refers to freezing a tissue sample in chilled Hexane. When I look in a chemical catalogue I find there are several formulas listed. Any direction on which one to use would be greatly appreciated. Thank you. Dorothy Dorothy Murphy Traczyk Murphy-Traczyk & Associates LLC PO Box 602 Point Pleasant, NJ 08742 _doro...@mtahistology.com_ (mailto:doro...@mtahistology.com) www.mtahistology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] optimal IHC thickness
Hi everyone, I am staining lymph nodes for staging of breast cancer with CKAE1/AE3 and would like to know what everyone is cutting this type of slide at. I searched the archives and my understanding is that, for IHC, how thick the sections are cut is not necessarily critical. I typically cut all IHC at 4μm and I want to achieve the best results possible. Also, on a side note, the Data Sheet suggests using Trypsin, but I have been using Pepsin. Any comments or suggestions about time, quality of Trypsin vs. Pepsin? Thank you, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Stain Disposal
Sally, the "Hazardous Materials in the Histopathology Laboratory " Regulations,, Risks, Handling and Disposal is a nice reference book to have. It can be purchased from Anatech LTD 1-800-ANATECH Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University 1800 Denison Ave Manhattan Ks 66506 >>> "Breeden, Sara" 8/12/2009 8:51 AM >>> Looking for a one-source listing of how to dispose of stains (i.e., specials - carbol fuchsin, iodine, Tartrazine, etc.). Is there such a list or reference? Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
