[Histonet] RE: EDTA and DNA damage, EDTA decalcification without fixation
You wrote: Does anyone know whether EDTA destroys DNA during decalcifying process and whether it would be better to fix the sample before adding EDTA? My samples are natural coral cubes of 3mm. It is carbonate calcium. The coral cubes are seeded with mesenchymal stem cells. I need to extract DNA in order to evaluate cell proliferation. *** EDTA is the recommended decalcification per many publications on the subject especially for ISH. You need to get this publication from J Histochem Cytochem 47(5)703-309, 1999 as it may direct you to a protocol for what you are doing. Effect of Bone Decalcification Procedures on DNA In Situ Hybridization and Comparative Genomic Hybridization: EDTA Is Highly Preferable to a Routinely Used Acid Decalcifier Janneke C. Alers, Pieter-Jaap Krijtenburg, Kees J. Vissers, and Herman van Dekken Also, bone does NOT have to be fixed prior to decalcification with EDTA. I These publications used fresh calcified bone, and decalcified with EDTA using some stringent methods for performing frozen sections. J Histochem & Cytochem 51(1):5-14, 2003 http://www.jhc.org Simultaneous Detection of EGFP and Cell Surface Markers by Fluorescence Microscopy in Lymphoid Tissues Kim L. Kusser and Troy D. Randall J Histochem Cytochem S. Mori et al, 36(1)111-117, 1988 There are a large number of publications about DNA and RNA and the effects of EDTA and other decalcifiers on the nucleic acids. I did a search and brought up no less than 17 articles although did not dig through all of them. I presume you are making sections prior to extraction of DNA from EDTA treated coral? Laser Capture microdissection?? Good luck you your project. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used Leitz 1600 Saw Microtome ?
Please contact me. Thanks! Vicki Kalscheur Department of Surgical Sciences School of Veterinary Medicine University of Wisconsin 2015 Linden Drive Madison, WI 53706-1102 Phone: 608-262-8534 FAX: 608-263-7930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Granular appearance to chromagen
Has anyone experienced a granular appearance to their chromagen pattern? I just did a run with Vector's NovaRed and all the slides show "staining" but on higher power, the appearance of the chromagen is granular (like it broke down?) I never had this problem with DAB and this is the second time I have experienced it with NovaRed. I use their kit, so after the addition of each component, I vortex to make sure it is well-mixed. Could this be the problem? Please advise. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] mtm p16 protocol
Unfortunately, there is no just purchasing the antibody. They package it with a negative control serum at the very least and if you wish to purchase the larger size, it comes with all the detection to perform it. I only needed the antibody and was told I couldn't purchase just that. It really increases the price when you have to purchase items that you don't need and drops the profit margin. Jodie Robertson, HT(ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor Chico, CA 95926 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of newacct391...@aol.com Sent: Friday, September 25, 2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mtm p16 protocol Hi everyone, We will soon be purchasing p16 from mtm laboratory. If i just want buy the antibody what is the protocol for using a decloaker chamber and Dako's retrieval solution? The directions say to use a waterbath but we use DC chambers. What temp. can i use and for how long? Any help would be greatly appreciated Thank you, Carol Schultz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HELP - p16 protocol
Hi everyone, We will soon be purchasing p16 from mtm laboratory. If i just want buy the antibody what is the protocol for using a decloaker chamber and Dako's retrieval solution? The directions say to use a waterbath but we use DC chambers. What temp. can i use and for how long? Any help would be greatly appreciated Thank you, Carol Schultz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mtm p16 protocol
Hi everyone, We will soon be purchasing p16 from mtm laboratory. If i just want buy the antibody what is the protocol for using a decloaker chamber and Dako's retrieval solution? The directions say to use a waterbath but we use DC chambers. What temp. can i use and for how long? Any help would be greatly appreciated Thank you, Carol Schultz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: Slide and Block Disposal
I heard a few bad things about it...(not that I have ever used it)... But when you have tissues that had prions (that nothing but heat kills), its not a good idea to crush the slides. They need to get melted or what ever is done to sterilize them first... anyone else know about this? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, September 25, 2009 8:27 AM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Slide and Block Disposal Tim, I was just thinking of that gizmo, but couldn't remember its name. They are also available for rent so that one could do their needed volume without having to keep such a thing around. Our intitution would be a prime candidate for the renting option. Plus, they look like they would be "fun" to see work! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, September 25, 2009 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Slide and Block Disposal Norm, if someone is really serious about destroying their slides, they could check out the SlydeEater. I haven't seen it for real but it appears to do an impressive job! http://www.ramflat.com/slydeater.html Tim Morken UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, September 24, 2009 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Disposal Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dako refursbishing in Southern California
Rueben, Try Pacific Southwest Lab Equipment Larry Fox 2384 La Mirada Drive Vista, CA 92081 (760) 295-1842 la...@psl-equip.com They should be able to help you. Jerry Helisek US Laboratory Supplies, LLC 919-264-7964 je...@uslabsupplies.com > Date: Fri, 25 Sep 2009 09:44:02 -0700 > From: ruebenjcar...@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dako refursbishing in Southern California > > Hi all. Does anyone know of a company that will service/refurbish a Dako IHC > stainer? I've tried contacting IMEB but cannot obtain a response despite > several messages. Any help would be appreciated. > > Thanks. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Insert movie times and more without leaving Hotmail®. See how. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Janella Seaton/WLGORE is out of the office.
I will be out of the office starting 09/25/2009 and will not return until 09/28/2009. I will respond to your message when I return. Any histology-related requests or questions can be sent to: histol...@wlgore.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide & Block disposal
Block disposal is easy for us. We dump 1 box of blocks each week or so right into the tubs we use for tissue disposal. Then the biohazard people haul it away. As for slides: We use biohazard sharps containers and have that hauled away with the other biohazardous materials. We just dump a small amount at a time so we don't have a huge disposal bill all at once. Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako refursbishing in Southern California
Hi all. Does anyone know of a company that will service/refurbish a Dako IHC stainer? I've tried contacting IMEB but cannot obtain a response despite several messages. Any help would be appreciated. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraform cassettes
Has anyone had any experience using paraform cassettes? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Does EDTA destroy DNA during decalcifying process?
Any decalcifying process, either acid or chelating, should always be conducted after the subject if fixed. René J. --- On Fri, 9/25/09, Martina Sladkova wrote: From: Martina Sladkova Subject: [Histonet] Does EDTA destroy DNA during decalcifying process? To: histonet@lists.utsouthwestern.edu Date: Friday, September 25, 2009, 11:36 AM Hello ! Does anyone know whether EDTA destroys DNA during decalcifying process and whether it would be better to fix the sample before adding EDTA? My samples are natural coral cubes of 3mm. It is carbonate calcium. The coral cubes are seeded with mesenchymal stem cells. I need to extract DNA in order to evaluate cell proliferation. Thank you very much in advance, Martina SLADKOVA PhD student LABORATOIRE DE BIOINGENIERIE ET BIOMECANIQUE OSTEO-ARTICULAIRES (B2OA) CNRS UMR 7052 Faculté de médecine Lariboisière Saint-Louis Université Paris 7 10 avenue de Verdun 75010 PARIS Tél.: 0033 01 57 27 86 84 Fax : 0033 01 57 27 85 71 Email: martina_sladk...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: Slide and Block Disposal
Anyone in interested in SlydEater information can contact me directly at 800-233-3271. Also, we will have a SlydEater on display in the Creative Waste Solutions booth (#839) at the upcoming NSH meeting in Birmingham. Mark Griffith Vyleater/SlydEater Product Manager S&G Enterprises, Inc. 800-233-3721 m...@slydeater.com or m...@vyleater.com http://www.SlydEater.com Date: Fri, 25 Sep 2009 09:26:38 -0600 From: "O'Donnell, Bill" To: "Morken, Tim" , histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Slide and Block Disposal Cc: Tim, I was just thinking of that gizmo, but couldn't remember its name. They are also available for rent so that one could do their needed volume without having to keep such a thing around. Our intitution would be a prime candidate for the renting option. Plus, they look like they would be "fun" to see work! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, September 25, 2009 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Slide and Block Disposal Norm, if someone is really serious about destroying their slides, they could check out the SlydeEater. I haven't seen it for real but it appears to do an impressive job! http://www.ramflat.com/slydeater.html Tim Morken UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, September 24, 2009 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Disposal Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Does EDTA destroy DNA during decalcifying process?
Hello ! Does anyone know whether EDTA destroys DNA during decalcifying process and whether it would be better to fix the sample before adding EDTA? My samples are natural coral cubes of 3mm. It is carbonate calcium. The coral cubes are seeded with mesenchymal stem cells. I need to extract DNA in order to evaluate cell proliferation. Thank you very much in advance, Martina SLADKOVA PhD student LABORATOIRE DE BIOINGENIERIE ET BIOMECANIQUE OSTEO-ARTICULAIRES (B2OA) CNRS UMR 7052 Faculté de médecine Lariboisière Saint-Louis Université Paris 7 10 avenue de Verdun 75010 PARIS Tél.: 0033 01 57 27 86 84 Fax : 0033 01 57 27 85 71 Email: martina_sladk...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: Slide and Block Disposal
Tim, I was just thinking of that gizmo, but couldn't remember its name. They are also available for rent so that one could do their needed volume without having to keep such a thing around. Our intitution would be a prime candidate for the renting option. Plus, they look like they would be "fun" to see work! William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, September 25, 2009 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Slide and Block Disposal Norm, if someone is really serious about destroying their slides, they could check out the SlydeEater. I haven't seen it for real but it appears to do an impressive job! http://www.ramflat.com/slydeater.html Tim Morken UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, September 24, 2009 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Disposal Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Slide and Block Disposal
Norm, if someone is really serious about destroying their slides, they could check out the SlydeEater. I haven't seen it for real but it appears to do an impressive job! http://www.ramflat.com/slydeater.html Tim Morken UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, September 24, 2009 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Disposal Dear Histonetters, I think everyone on the Histonet would like to hear about all the creative, cost effective, and HIPPA-compliant ways labs can dispose of slides and blocks that have exceeded their retention limits. Does anyone use an outside company that disposes of glass slides and/or paraffin blocks, or is there equipment to be rented/purchased that would provide a solution to everyone's slide and block disposal challenge? We would all like to hear of your innovative or not so innovative solutions to this challenge. Thank you in advance for your solutions. Norm Burnham ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide and Block Disposal
We do the following for slide and block disposal: Slides are discarded into a rigid, puncture-proof biohazard sharps container and discarded with the regular trash. All paraffin blocks are discarded into the regular trash, as well. I believe I once read that "Joe the Toe" Nocito remarked that paraffin blocks are not hazardous (infectious) and to prove his point, he licked the block! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide and Block Disposal
We use SAFETY KLEEN... they are inexpensive and have the HIPPA thing down pat. In fact, they also take care of all our waste. They truly are the best company I have ever used, and Dan goes out of his way to help his clients!!! Contact info: DAN WIENHOLZ Manager 707-584-0415 WWW.Safety-Kleen.com Maria Katleba HT (ASCP) MS Queen of the Valley Medical Center Napa CA 707-257-4076 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lorraine Cornett Sent: Friday, September 25, 2009 6:57 AM To: norm.burn...@propath.com; Histonet Listserve Subject: RE: [Histonet] Slide and Block Disposal Could everyone reply to all on this one, i have some of the same questions. Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 > Date: Thu, 24 Sep 2009 20:26:05 -0500 > From: norm.burn...@propath.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide and Block Disposal > > Dear Histonetters, > I think everyone on the Histonet would like to hear about all the creative, > cost effective, and HIPPA-compliant ways labs can dispose of slides and > blocks that have exceeded their retention limits. > Does anyone use an outside company that disposes of glass slides and/or > paraffin blocks, or is there equipment to be rented/purchased that would > provide a solution to everyone's slide and block disposal challenge? > We would all like to hear of your innovative or not so innovative solutions > to this challenge. > Thank you in advance for your solutions. > Norm Burnham _ Bing(tm) brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD31 Mouse Liver Staining
Hi Jeff, I have had best results using Zinc Fixative for CD31 (PECAM1) staining in mouse tissues. You can either post fix cryosections or immersion fix tissues trimmed thin (~4mm). Zinc Fixative is available from BD/Pharmingen or you can prepare it. Email me if you want details on preparing it yourself. Good luck, Jameel >>> 9/24/2009 8:14:42 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Orienting GI biopsies during embedding (Rene J Buesa) 2. Re: Histobath Frozen Sections (Robert Richmond) 3. Re: Orienting GI biopsies during embedding (Robert Richmond) 4. Heidelberger Pinzette (Gudrun Lang) 5. MSI testing (Richard Cartun) 6. Steiner and Steiner (Wahlberg, Nikki) 7. SOX10 (Martin, Erin) 8. RE: Steiner and Steiner (Jodie Robertson) 9. RE: MSI testing (Weems, Joyce) 10. Need a NSH S/C roommate (Marquisha Paul) 11. CD31 Mouse Liver Staining (JEFFREY S HARDING) 12. FW: [Histonet] Steiner and Steiner (Wahlberg, Nikki) 13. Guniea pig anti-Doublecortin & Goat-Guinea pig secondary antibody on rat tissue ( ti fei ) 14. MOLECULAR LAB QUESTION (Maria Katleba) 15. cap survays (Gonzales, Edith) 16. Re: Steiner and Steiner (Rene J Buesa) 17. RE: cap survays (Morken, Tim) 18. Re: ISH on decaled bone (Johnson, Teri) 19. Re: License (Johnson, Teri) 20. B Plus fixative on bone marrow cores (Della Speranza, Vinnie) 21. RE: Orienting GI biopsies during embedding (Tony Henwood) 22. RE: Steiner and Steiner (Tony Henwood) 23. RE: B Plus fixative on bone marrow cores (Lee & Peggy Wenk) 24. RE: cap survays (Tony Henwood) 25. See you at the NSH (Tony Henwood) 26. Rhodanine Copper stain (Carrie Disbrow) -- Message: 1 Date: Thu, 24 Sep 2009 10:11:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Orienting GI biopsies during embedding To: "histonet@lists.utsouthwestern.edu" ,ssylv...@cinci.rr.com Message-ID: <500291.35986...@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There is a very fine Technical Note by I. Dimenstein in the last issue of the JOH that can help you solve this problem. René J. --- On Thu, 9/24/09, ssylv...@cinci.rr.com wrote: From: ssylv...@cinci.rr.com Subject: [Histonet] Orienting GI biopsies during embedding To: "histonet@lists.utsouthwestern.edu" Date: Thursday, September 24, 2009, 12:45 PM Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology Cincinnati Children's Hospital ssylv...@cinci.rr.com (513)803-0741 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Thu, 24 Sep 2009 13:15:16 -0400 From: Robert Richmond Subject: [Histonet] Re: Histobath Frozen Sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I've posted a number of times about the Histobath. I don't know anything new besides the stuff I've already posted. Melanie S. White, MT(ASCP) asks: >>P.S. Are there any other Samurai Pathologists available? We need one.<< Hey, find me a way to get a South Carolina medical license at the age of 70 and we'll talk! Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Thu, 24 Sep 2009 13:27:10 -0400 From: Robert Richmond Subject: [Histonet] Re: Orienting GI biopsies during embedding To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Sabina Sylvest at Cincinnati Children's Hospital asks: >>We are having a heck of a time orienting GI biopsies but esophageal biopsies >>in particular, during embedding. If anyone could share their secret for >>getting these tiny specks of semi-transparent tissue oriented, we would >>greatly appreciate it.<< At the very least, I'd use an OptiVISOR magnifier with 3 diopter or 4 diopter lenses - I've mentioned this item on Histonet before. See www.doneganoptical.com/optivisor.php - Donegan doesn't retail, but you can get the item from Amazon. If I had one, I'd want to use a stereo dissecting microscope with 10 and 20 powe
[Histonet] fluorescent antibodies for IHC
Dear Histonetters. I've been given the challenge of finding a variety of fluorochrome conjugated antibodies for IHC, however I'm having a great deal of trouble finding them. We basically want a fluorescent primary antibody that can be visualized instantly without use of a secondary etc. We would like antibodies to bind to Osteocalcin, Alkaline Phosphatase, Periostin, TRAP 5B, Cathepsin-K and N-Cadherin. If anyone has experience using fluorescent antibodies for the above or know of anyone who produces them I would be most grateful if you could let me know. Thanks Julia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ultra-Low Temp. Upright Freezer
While at my previous company, we switched to all Sanyo products because we found them superior to others. They also have the best customer service, at least here in Wisconsin. Do not buy Norlake or So-Low Kris Olsen Histology Manager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, September 17, 2009 8:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ultra-Low Temp. Upright Freezer Hi all! I'm hoping to get some help RE: purchasing a new ultra-low temp. upright freezer. I've tried searching Histonet, but many of the posts are a few years old, and my guess is that the products have changed a bit over the years. My lab is looking to purchase a large (absolute minimum size of 17 cu.ft., but preferably much larger), and definitely upright (not chest) -80. Unfortunately, since this is my first foray into the world of lab freezers, I know very little about them. I have discovered that Thermo's Revco and Sanyo freezers seem to be popular. Obviously we're looking for a freezer that is as large and efficient as possible. Insulation and the freezer's compressor(s) are most important. I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.) freezer seems quite nice - small exterior footprint and his sales pitch regarding the vacuum insulation (therefore denser insulation), insulated inner doors, and supposed minimized carbon footprint was quite convincing. I still plan on talking to Revco's rep. RE: their Elite and Ultima freezers, though my guess is that their sales pitch will be just as convincing. Since you can't depend only on sales pitches, I'm depending on colleagues as well Does anyone have any experience with either the Sanyo or Revco freezers (or any others that they'd like to recommend)? Any and all positive and negative feedback would be greatly appreciated! I'm hoping to get comments regarding: - efficiency - how often service is needed due to failures - are the alarms sensitive enough or maybe too sensitive - how loud the freezer is when the compressor is on - etc.. As always, please remember to Reply All. Thank you in advance for your help! I look forward to hearing everyone's suggestions. Lucie UCSD lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello. Histonetters. I have recently used zinc fixed tibia and calvaria for the staining of CD31. My CD31 does seem to have worked, however there seems to be marrow loss and digestion of the marrow also. I zinc fixed the bones for 48 hours and then used EDTA pH 7 for decalcification for 2 weeks. I am unsure whether I have fixed the bones for too long, whether the decal has been affected by the zinc fix (as the bones were "jelly-like" before processing) or whether the decalcification needs shortening when using zinc fixation. If anyone has any experience in using zinc fixation on tibia for Immuno staining and can offer some advice that would be much appreciated. Thanks Julia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD31 Mouse Liver Staining
Hi Jeff, I have had best success using Zinc Fixative for CD31 (PECAM1) staining in mouse tissues. It is important that the tissue is trimmed thin (~4mm) for proper fixation and recommended fixation time is 24-48 hrs. The Zinc Fixative is available from BD/Pharmingen or you can prepare it. Email me if you want details on preparing it yourself. Good luck, Jameel Message: 11 Date: Thu, 24 Sep 2009 13:46:16 -0500 From: JEFFREY S HARDING Subject: [Histonet] CD31 Mouse Liver Staining To: histonet@lists.utsouthwestern.edu Message-ID: <7050ac4f65505.4abb7...@wiscmail.wisc.edu> Content-Type: text/plain; charset=us-ascii Hello, I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections. I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal. My question is regarding fixation methods IMMEDIATELY after dissection. Does anyone have a good summary of fixation methods prior to freezing and their effects on CD31 staining? Does the exact fixation even matter that much? Everyone seems to have something different. Thanks! Jeff ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide and Block Disposal
Could everyone reply to all on this one, i have some of the same questions. Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 > Date: Thu, 24 Sep 2009 20:26:05 -0500 > From: norm.burn...@propath.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide and Block Disposal > > Dear Histonetters, > I think everyone on the Histonet would like to hear about all the creative, > cost effective, and HIPPA-compliant ways labs can dispose of slides and > blocks that have exceeded their retention limits. > Does anyone use an outside company that disposes of glass slides and/or > paraffin blocks, or is there equipment to be rented/purchased that would > provide a solution to everyone's slide and block disposal challenge? > We would all like to hear of your innovative or not so innovative solutions > to this challenge. > Thank you in advance for your solutions. > Norm Burnham _ Bing™ brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Rhodanine Copper stain
Yes, copper can be successfully demonstrated in 3 micron sections using this method. Rena Fail --- On Thu, 9/24/09, Carrie Disbrow wrote: From: Carrie Disbrow Subject: [Histonet] Rhodanine Copper stain To: histonet@lists.utsouthwestern.edu Date: Thursday, September 24, 2009, 8:12 PM Hello! I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of tissue core biopsies are cut at 3 microns and if possible we would not like to cut the block again. Thanks for your input. Thank you, Carrie Disbrow, HT ASCP (soon to be BS, HTL ASCP :-)) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Rhodanine Copper stain
The procedure calls for thicker sections because usually the amounts of copper are small, very dispersed and are easily seen in thicker sections. On the other hand the Rhodamine copper stain is not very sensitive. I always used Timm's method which is much more sensitive. René J. --- On Thu, 9/24/09, Carrie Disbrow wrote: From: Carrie Disbrow Subject: [Histonet] Rhodanine Copper stain To: histonet@lists.utsouthwestern.edu Date: Thursday, September 24, 2009, 8:12 PM Hello! I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of tissue core biopsies are cut at 3 microns and if possible we would not like to cut the block again. Thanks for your input. Thank you, Carrie Disbrow, HT ASCP (soon to be BS, HTL ASCP :-)) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] See you at the NSH
I'll be looking for you! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, September 24, 2009 7:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] See you at the NSH Hi all, Just a note to say that I will be leaving next week for Birmingham Alabama for the NSH conference. It has taken me 30 years to get to a NSH and I am really looking forward to it. I hope to see you all (I will be the old, short, bald guy with the Aussie accent) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA - * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ASCP CMP continuing education credits
According to the ASCP CMP booklet, as a HT you need 36 hours (points): http://ascp.org/pdf/CMPBooklet.aspx - 1 point in Safety - 2 points minimum in area of your certification (that would be Histotechnology) - Remaining points in area of specialty, management, education or other related areas of interest So it looks like you still need 1 hour in safety, and maybe, just to be on the safe side, 2 hours specifically related to histotechnology. Now, as to whether the Anderson CE course would qualify - has any histonetter used them? Has ASCP accepted them? The closest criteria that I could fit them under would be: 4. Teleconference, subscription, or online self-instructional courses-These courses are acceptable based on any of the following criteria: a. ACCME, CMLE, ACCENT, PACE credits are awarded, or b. they are offered by a professional society (including state, regional or local chapter), or c. the course is accepted by a state licensing board, or d. the course is offered through a university or college. OR 1. Formal continuing education courses-These courses may be completed through the programs/organizations listed on the chart as well as through other professional societies such as those listed under Suggested List of Providers on page 6. Courses offered by state/regional/local societies and chapters are acceptable as well as courses offered through the continuing education departments of colleges and universities. Courses offered by organizations approved by state licensing boards are also acceptable. I don't know this company/organization, so I don't know if it would be acceptable to ASCP. And ASCP doesn't want people contacting them. "Please Note: Because of the large volume of continuing education courses available, the Board of Registry will not respond to requests for approval of individual programs or courses. If the program/course meets the criteria listed, it will be accepted for CMP points. Program provider must assign points or contact hours." Keep us informed. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kmcn...@gmail.com Sent: Thursday, September 24, 2009 10:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP CMP continuing education credits I am attempting to renew my HT ASCP certification for the first time and I was wondering if taking the Anderson Continuing Education course (Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications-they list it as providing 36 hours) would fulfill the requirement completely. I am also certified by the FL BOH and have taken HIV and Medical errors for a few points to meet the states renewal requirements. I only have a couple months until renewal is required so any information would be greatly appreciated! Thanks, M. Mcneta ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
