[Histonet] frozen sections for in situ hybridization

[Anand Vasudevan]

Hi,
I have obtained fresh rat brains (not fixed) that have been directly
snap-freezed in isopentane and stored at -80C. I plan to section 20um thick.
I want to know if there is way to store the cut sections before I carry out
the in situ hybridization without thawing and freezing?
taht is, the cut sections will be frozen but when placed on slides, but they
will thaw before I can store them in the fridge (either -20C or -80C) and
then before conducting my experimemt i will have to thaw it again.

Thanks,
Anand Vasudevan
Nanyang Technological University
Singapore
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[Histonet] (no subject)

[Moody, Robert]

Hi all can some one please give me the web site to apply for newyork state 
license
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[Histonet] Licensing for Massachusetts?

[Michelle MacVeigh-Aloni]

Hi all,

I live in Los Angeles and have HT and HTL from CA.

I would like to move to Cape Cod in couple of years. 

I know that one needs a special Florida license to work in Fl, but do I need a 
special license from Massachusetts to be able to do histology there?

I would appreciate any info
Michelle Aloni

USC Keck School of Medicine
Los Angeles, CA
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[Histonet] Re: Frozen Myocardium sections

[Michelle MacVeigh-Aloni]

The OCT cracks if the block remains in liquid N too long.

I use a plastic mold, which holds my tissue in the OCT.
Float the mold on the surface of the liquid N but pull it out of there while 
there is still a little liquid/clear OCT (about 6-7mm in diameter) in the 
middle of the forming block.
Put it on the counter (room temp.). As the block sits on the counter, for a 
minute or two, the freezing of the center takes place. The block will never 
crack if it is pulled out of the OCT on time.

Michelle Aloni MS HTL ASCP
USC Keck School of Medicine
Los Angeles, CA
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[Histonet] (no subject)

[Santiago, Albert]

Hello fellow histonetters, I was hoping someone can tell me how long it
is required to store patient serum for indirect immunofluorescence and
patient tissue frozen in OCT medium for direct immunofluorescence. Also,
how long do we store the slides produced by these two procedures? Also,
the source where I can refer to. Thank you very much for your help. My
email address is,  albert.santi...@uphs.upenn.edu 

 

Albert Santiago, HT(ASCP)

Lab Supervisor

Dermatopathology

215-662-6539-office

215-662-6150-fax

 



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[Histonet] Sudan Black stain for fluid smear

[Maria Katleba]

Anyone know of a Sudan Black technique for peritoneal fluid smear ???  Or if 
it's even possible



Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689




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RE: [Histonet] Licensing?

[Jaime Plata]

Contact State of Department of Health Division of Medical Quality Assurance.
Be careful in your selection. I did move from Va down here and the housing 
(realstate) and pay hourly it is no as good we thought.
Jaime E Plata
BS. MT. HTL (ASCP)
Clinical Lab Supervisor Supervisor
State of Florida Department of Health

--- On Wed, 10/7/09, Feher, Stephen  wrote:


From: Feher, Stephen 
Subject: RE: [Histonet] Licensing?
To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, 
"Komal Gada" 
Date: Wednesday, October 7, 2009, 10:08 AM


Here's the link to the Fla DOH Licensure Offices.

http://www.doh.state.fl.us/mqa/med-boards.html 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 07, 2009 8:11 AM
To: histonet@lists.utsouthwestern.edu; Komal Gada
Subject: Re: [Histonet] Licensing?

The best thing for you to do is to visit the Florida society for 
histotechnology website. Google the name.
René J.

--- On Tue, 10/6/09, Komal Gada  wrote:


From: Komal Gada 
Subject: [Histonet] Licensing?
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 6, 2009, 9:17 PM


Hello all,
I am an ASCP certified Histotechnician (HT), i also have NY state license.Can 
anyone suggest the best way to obtain a Florida license?

Thanks,

I appreciate your replies.
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[Histonet] tris buffer

[Perry, Margaret]

I am trying a protocol that uses 0.1M Tris/0.15M sodium chloride pH7.5, which 
is what I normally use.  At another step in the protocol it says to wash with 
0.05M Tris buffer pH 7.5.
What do I use to make the 0.05M Tris buffer?  Is it a combination of Tris HCl 
and Tris base without sodium chloride?
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[Histonet] regulations or procedures for "pre-labeling slides"???

[jcarpenter764]

Does anyone know where I can find the protocal or know the protocal for 
prelabeling slides before cutting the tissue?
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[Histonet] Pathology Manager position with Providence in Portland, Oregon

[Fletcher, Annette M]

Dear Histonetters,

(no recruiters please)


Providence Health and Services is seeking a Pathology Manager in Portland, OR 
to oversee three supervisors who manage Histo, Cyto and Pathology 
Transcription.  This is a new position!  There are about 60 Full Time employees 
working under these Supervisors.  The hiring range for this will be somewhere 
between 95K and 100K (possible room for negotiation).  Here are the job 
description and requirements:



General Summary: The position of Pathology Manager is one primarily focused on 
delivering pathology related service commitments to six hospitals and numerous 
outreach customers within the State of Oregon by coordinating the technical and 
support sections of cytopathology processing and screening, histology, surgical 
pathology grossing and transcription with the professional physician staff 
(pathologists).



Position requires a Bachelors degree in healthcare or related field with 
additional formal training in finance, economics, purchasing and operations 
management. A minimum of 10 year's progressive medical/healthcare management 
experience, in a high volume facility, preferably with bottom line 
accountability in a for profit environment. Familiarity with all aspects of 
department operations, including cost effective management of labor and 
supplies. Familiarity with workflow and operational processes between technical 
and non-technical functions and physician staff. Demonstrated ability to 
achieve operational improvement leading to margin enhancement. Ability to 
identify root causes for operational and financial variances and to remediate.

Preferred: MBA.



Please call me directly at 503-215-5840 or email me with any questions.



Kind Regards,

Annette M Fletcher

Senior Recruiter

Providence Health & Services, Employment

1235 NE 47th Avenue - Suite 200

Portland, OR 97213

T: 503-215-5840

F: 503-215-5770

annette.fletc...@providence.org


Kind Regards,
Annette M Fletcher
Senior Recruiter
Providence Health & Services, Employment
1235 NE 47th Avenue - Suite 200
Portland, OR 97213
T: 503-215-5840
F: 503-215-5770
annette.fletc...@providence.org



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Re: [Histonet] Histology positions

[Ben Spirto]

Hi;
I am looking for part-time (nights and weekends) position as a HTL or HT or
any lab position in Chicagoland area. Experience in immunohistochemistry.
Please reply to: brod...@gmail.com

Thank you

On Tue, Oct 6, 2009 at 11:35 AM, Paula Wilder  wrote:

>
> I have two full time Histology Technician positions available starting
> October 21st.  We are located at St. Joseph Medical Center in Towson, Md.
>  If anyone is interested, please contact Paula Wilder at 410-337-1741.
>  Thank you so much!
> _
> Hotmail: Powerful Free email with security by Microsoft.
>
> http://clk.atdmt.com/GBL/go/171222986/direct/01/___
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> Histonet@lists.utsouthwestern.edu
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>
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RE: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES

[Vacca Jessica]

I would like to know how you went about establishing the search fee, there are 
many times that these legal companies like Litigation management, Record trak, 
central records depository that just do a mass letter requesting records, and 
there is no pathology to be found, then they ask for you to sign and motorize 
their documents. I would absolutely love to tell them that there is a fee to 
search. Who established the fee your legal department or the lab? 
We charge 50 for the case, 10 per block after the first block, and 25 for 
additional cases with the 10 fee still applying for each additional block.

Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr
Brandon Fl 33511
(813) 571-5193
(813) 571-5169 FAX
  


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith Wanda
Sent: Wednesday, October 07, 2009 10:47 AM
To: sris...@mail.holyname.org; histonet-boun...@lists.utsouthwestern.edu; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, 
MEDICAL PURPOSES

Good Morning to All,
I have developed a fee schedule for legal cases and I have a form letter that I 
send to the offices before any slides are sent.  We only furnish 1 H&E and 2 
unstained slides per block, if tissue is adequate.  We never send original 
slides and we never send blocks.  My charges are:
$25.00 for looking up the case and up to 5 copies 
$12.50 per slide and a minimum of 3 slides per block
They pay any shipping other than US mail.  Sometimes the lawyer's office only 
wants reports and sometimes I have received checks for up to $375.00.

If a patient or institution request blocks for Continuation of Care, we either 
send the original slides with a request for them to be returned or we do recuts 
at no charge to the patient or the requesting institution.
Hope this helps!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
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attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sris...@mail.holyname.org
Sent: Wednesday, October 07, 2009 10:26 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, 
MEDICAL PURPOSES

Hi All,

I know this question has been asked before.  What is everyone doing about 
cutting and preparing slides and blocks for legal purposes as well as requests 
from other hospitals or patients. Is there any charges and how much are the 
charges?

Thanks in advance for your suggestions.

Nirmala Srishan

















__

I am seeking a Pathology Manager for a hospital system located in a great city 
in the Pacific NW.  The manager would oversee three supervisors who manage 
Histo, Cyto and Pathology Transcription.  There are about 60 Full Time 
employees working under these Supervisors.

The position of Pathology Manager is one primarily focused on delivering 
pathology related service commitments to six hospitals and numerous outreach 
customers  by coordinating the technical and support sections of cytopathology 
processing and screening, histology, surgical pathology grossing and 
transcription with the professional physician staff (pathologists).

For more details contact me at 574.633.1231.

Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com
j...@opssearchgroup.com
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY 
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Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007  
Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 
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Care, HealthGrad

RE: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL,MEDICAL PURPOSES

[Nita Searcy]

I have been charging for legal cases for years; other institutions there is no 
charge---patient carte issue. We have a $60.00 search fee ( transcription & 
histology expense offset) ; if we recut- $25.00 for each H&E; special stains- 
$50; immunohistochemistry- $75.00.
Nita

Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438

>>> "Rathborne, Toni"  10/7/2009 9:38 AM >>>
We do not charge for recuts or send outs. Our Medical Records department 
charges $1 per page for reports. Charging is something that we have discussed 
for many years, though. I am also interested in what other institutions doe. It 
takes a lot of time for packing and record keeping even if sending originals 
(for legal cases), not to mention the time to recut and stain and the cost to 
send them.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of
sris...@mail.holyname.org 
Sent: Wednesday, October 07, 2009 10:26 AM
To: histonet-boun...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu 
Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR
LEGAL,MEDICAL PURPOSES


Hi All,

I know this question has been asked before.  What is everyone doing about 
cutting and preparing slides and blocks for legal purposes as well as 
requests from other hospitals or patients. Is there any charges and how 
much are the charges?

Thanks in advance for your suggestions.

Nirmala Srishan

















__

I am seeking a Pathology Manager for a hospital system located in a great 
city in the Pacific NW.  The manager would oversee three supervisors who 
manage Histo, Cyto and Pathology Transcription.  There are about 60 Full 
Time employees working under these Supervisors.

The position of Pathology Manager is one primarily focused on delivering 
pathology related service commitments to six hospitals and numerous 
outreach customers  by coordinating the technical and support sections of 
cytopathology processing and screening, histology, surgical pathology 
grossing and transcription with the professional physician staff 
(pathologists).

For more details contact me at 574.633.1231.

Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com 
j...@opssearchgroup.com 
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY
___
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Healthcare, Ranked Sixth Nationally by  Modern Healthcare , 2008 Best Places to 
Work in New Jersey,  NJBIZ  - 2006, 2007, 2008 & 2009  Awards for Emergency, 
Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007  
Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 
2006, 2007 & 2008  Excellence Awards for Stroke, Gastrointestinal and Pulmonary 
Care, HealthGrades - 2008 & 2009  Chest Pain Center Accreditation, Society of 
Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and 
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Re: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES

[DKBoyd]

For legal cases we charge $25.00 per slide and send on their FedEx 
account.  We do not charge for patient referral work, it is considered a 
courtesy for using our facility.

Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical 
Center I 
200 Medical Park Boulevard I Petersburg, Va.  23805 I T: 804-765-5050 I F: 
804-765-5582 I dkb...@chs.net







sris...@mail.holyname.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/07/2009 10:27 AM

To
histonet-boun...@lists.utsouthwestern.edu, 

cc

Subject
[Histonet] CHARGING  FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL 
PURPOSES






Hi All,

I know this question has been asked before.  What is everyone doing about 
cutting and preparing slides and blocks for legal purposes as well as 
requests from other hospitals or patients. Is there any charges and how 
much are the charges?

Thanks in advance for your suggestions.

Nirmala Srishan

















__

I am seeking a Pathology Manager for a hospital system located in a great 
city in the Pacific NW.  The manager would oversee three supervisors who 
manage Histo, Cyto and Pathology Transcription.  There are about 60 Full 
Time employees working under these Supervisors.

The position of Pathology Manager is one primarily focused on delivering 
pathology related service commitments to six hospitals and numerous 
outreach customers  by coordinating the technical and support sections of 
cytopathology processing and screening, histology, surgical pathology 
grossing and transcription with the professional physician staff 
(pathologists).

For more details contact me at 574.633.1231.

Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com
j...@opssearchgroup.com
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY
___
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in Patient Care, American Nurses Credentialing Center 100 Best Places to 
Work in Healthcare, Ranked Sixth Nationally by  Modern Healthcare , 2008 
Best Places to Work in New Jersey,  NJBIZ  - 2006, 2007, 2008 & 2009 
Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. 
Power - 2006 & 2007  Distinguished Hospital Awards for Clinical 
Excellence, HealthGrades - 2005, 2006, 2007 & 2008  Excellence Awards for 
Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 
Chest Pain Center Accreditation, Society of Chest Pain Centers Primary 
Stroke Center Designation, The Joint Commission and NJ Department of 
Health and Human Services  Warning: The information contained in this 
message is privileged and CONFIDENTIAL and is intended only for the use of 
the addressee above. If you are not the intended recipient, you are hereby 
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RE: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES

[Smith Wanda]

Good Morning to All,
I have developed a fee schedule for legal cases and I have a form letter that I 
send to the offices before any slides are sent.  We only furnish 1 H&E and 2 
unstained slides per block, if tissue is adequate.  We never send original 
slides and we never send blocks.  My charges are:
$25.00 for looking up the case and up to 5 copies 
$12.50 per slide and a minimum of 3 slides per block
They pay any shipping other than US mail.  Sometimes the lawyer's office only 
wants reports and sometimes I have received checks for up to $375.00.

If a patient or institution request blocks for Continuation of Care, we either 
send the original slides with a request for them to be returned or we do recuts 
at no charge to the patient or the requesting institution.
Hope this helps!!!
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
recipient. If you are not the intended recipient of the email or any of its 
attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sris...@mail.holyname.org
Sent: Wednesday, October 07, 2009 10:26 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, 
MEDICAL PURPOSES

Hi All,

I know this question has been asked before.  What is everyone doing about 
cutting and preparing slides and blocks for legal purposes as well as requests 
from other hospitals or patients. Is there any charges and how much are the 
charges?

Thanks in advance for your suggestions.

Nirmala Srishan

















__

I am seeking a Pathology Manager for a hospital system located in a great city 
in the Pacific NW.  The manager would oversee three supervisors who manage 
Histo, Cyto and Pathology Transcription.  There are about 60 Full Time 
employees working under these Supervisors.

The position of Pathology Manager is one primarily focused on delivering 
pathology related service commitments to six hospitals and numerous outreach 
customers  by coordinating the technical and support sections of cytopathology 
processing and screening, histology, surgical pathology grossing and 
transcription with the professional physician staff (pathologists).

For more details contact me at 574.633.1231.

Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com
j...@opssearchgroup.com
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Holy Name Hospital  is the recipient of: Magnet Recognition for Excellence in 
Patient Care, American Nurses Credentialing Center 100 Best Places to Work in 
Healthcare, Ranked Sixth Nationally by  Modern Healthcare , 2008 Best Places to 
Work in New Jersey,  NJBIZ  - 2006, 2007, 2008 & 2009  Awards for Emergency, 
Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007  
Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 
2006, 2007 & 2008  Excellence Awards for Stroke, Gastrointestinal and Pulmonary 
Care, HealthGrades - 2008 & 2009  Chest Pain Center Accreditation, Society of 
Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and 
NJ Department of Health and Human Services  Warning: The information 
contained in this message is privileged and CONFIDENTIAL and is intended only 
for the use of the addressee above. If you are not the intended recipient, you 
are hereby notified that any disclosure, copying, distribution, or taking of 
any action in reliance on the content of this message is strictly prohibited. 
If you have received this communication in error, please notify the sender by 
replying to this message, and then delete it from your system.  
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RE: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES

[Rathborne, Toni]

We do not charge for recuts or send outs. Our Medical Records department 
charges $1 per page for reports. Charging is something that we have discussed 
for many years, though. I am also interested in what other institutions doe. It 
takes a lot of time for packing and record keeping even if sending originals 
(for legal cases), not to mention the time to recut and stain and the cost to 
send them.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of
sris...@mail.holyname.org
Sent: Wednesday, October 07, 2009 10:26 AM
To: histonet-boun...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Subject: [Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR
LEGAL,MEDICAL PURPOSES


Hi All,

I know this question has been asked before.  What is everyone doing about 
cutting and preparing slides and blocks for legal purposes as well as 
requests from other hospitals or patients. Is there any charges and how 
much are the charges?

Thanks in advance for your suggestions.

Nirmala Srishan

















__

I am seeking a Pathology Manager for a hospital system located in a great 
city in the Pacific NW.  The manager would oversee three supervisors who 
manage Histo, Cyto and Pathology Transcription.  There are about 60 Full 
Time employees working under these Supervisors.

The position of Pathology Manager is one primarily focused on delivering 
pathology related service commitments to six hospitals and numerous 
outreach customers  by coordinating the technical and support sections of 
cytopathology processing and screening, histology, surgical pathology 
grossing and transcription with the professional physician staff 
(pathologists).

For more details contact me at 574.633.1231.

Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com
j...@opssearchgroup.com
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY
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[Histonet] CD3 on mouse tissue

[Jennifer Campbell]

Hi Histonetters,
 
  I'm trying to work up CD3 on mouse tissue.  I knew I would have to get
a mouse-on-mouse kit since CD3 is a rabbit polyclonal but do you use
some sort of rodent kit to eliminate background staining?  My secondary
antibody is part of a multilink kit from Biogenex and is capable of
recognizing both mouse and rabbit primary antibodies.  So, I'm afraid
that it is recognizing endogenous antibodies in my mouse tissue.  Any
recommendations?  
 
Thanks,
 
Jennifer
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[Histonet] CHARGING FOR SLIDES TO BE CUT AND SENT OUT FOR LEGAL, MEDICAL PURPOSES

[srishan]

Hi All,

I know this question has been asked before.  What is everyone doing about 
cutting and preparing slides and blocks for legal purposes as well as 
requests from other hospitals or patients. Is there any charges and how 
much are the charges?

Thanks in advance for your suggestions.

Nirmala Srishan

















__

I am seeking a Pathology Manager for a hospital system located in a great 
city in the Pacific NW.  The manager would oversee three supervisors who 
manage Histo, Cyto and Pathology Transcription.  There are about 60 Full 
Time employees working under these Supervisors.

The position of Pathology Manager is one primarily focused on delivering 
pathology related service commitments to six hospitals and numerous 
outreach customers  by coordinating the technical and support sections of 
cytopathology processing and screening, histology, surgical pathology 
grossing and transcription with the professional physician staff 
(pathologists).

For more details contact me at 574.633.1231.

Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com
j...@opssearchgroup.com
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY
___
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Holy Name Hospital  is the recipient of: Magnet Recognition for Excellence in 
Patient Care, American Nurses Credentialing Center 100 Best Places to Work in 
Healthcare, Ranked Sixth Nationally by  Modern Healthcare , 2008 Best Places to 
Work in New Jersey,  NJBIZ  - 2006, 2007, 2008 & 2009  Awards for Emergency, 
Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007  
Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 
2006, 2007 & 2008  Excellence Awards for Stroke, Gastrointestinal and Pulmonary 
Care, HealthGrades - 2008 & 2009  Chest Pain Center Accreditation, Society of 
Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and 
NJ Department of Health and Human Services  Warning: The information 
contained in this message is privileged and CONFIDENTIAL and is intended only 
for the use of the addressee above. If you are not the intended recipient, you 
are hereby notified that any disclosure, copying, distribution, or taking of 
any action in reliance on the content of this message is strictly prohibited. 
If you have received this communication in error, please notify the sender by 
replying to this message, and then delete it from your system.  
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Re: [Histonet] frozen myocardium sections

[Geoff McAuliffe]

You could but it would be too cold and might crack the tissue. 
Isopentane becomes a solid and mitagates, for lack of a better word, the 
coldness of  liquid nitrogen.
A good alternative would be to cool the rod with dry ice which is only 
about -80C or so.


Geoff

Merced M Leiker wrote:
Hi Geoff, just curious...could you not just plunge the rod directly 
into the liquid N to cool it?


Regards,
Merced

--On Tuesday, October 06, 2009 10:32 AM -0400 Geoff McAuliffe 
 wrote:



1. Cool the isopentane with liquid N. Put a metal rod (aluminum, brass,
copper) in the chilled isopentane. Wait for the rod to cool.
2. Put the tissue+OCT on a metal object disk, microtome chuck or even a
small metal plate.
3. Put the metal supporting the tissue on the chilled metal rod, the
tissue will freeze rapidly without cracking or touching the isopentane.
Voila!
Put the tissue in the cryostat at the appropriate temperature for
sectioning and have some coffee while the tissue "warms up" to cutting
temperature.

Geoff


Jean-Martin Lapointe wrote:

Hi all,

for a study we are freezing myocardium sections in OCT immersed 
directly

in liquid nitrogen, without isopentane, because apparently isopentane
quenches the fluorescence of the cells we need to detect in the tissue.
Unfortunately the blocks tend to crack after freezing. Does anyone have
a suggestion to avoid cracking ?

thanks



__

Jean-Martin Lapointe

AccelLAB Inc

jm.lapoi...@accellab.com 







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--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 mcaul...@umdnj.edu
**



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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.






--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcaul...@umdnj.edu

**



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RE: [Histonet] Licensing?

[Feher, Stephen]

Here's the link to the Fla DOH Licensure Offices.

http://www.doh.state.fl.us/mqa/med-boards.html 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 07, 2009 8:11 AM
To: histonet@lists.utsouthwestern.edu; Komal Gada
Subject: Re: [Histonet] Licensing?

The best thing for you to do is to visit the Florida society for 
histotechnology website. Google the name.
René J.

--- On Tue, 10/6/09, Komal Gada  wrote:


From: Komal Gada 
Subject: [Histonet] Licensing?
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 6, 2009, 9:17 PM


Hello all,
I am an ASCP certified Histotechnician (HT), i also have NY state license.Can 
anyone suggest the best way to obtain a Florida license?

Thanks,

I appreciate your replies.
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[Histonet] Path lab manager position

[jeri]

I am seeking a Pathology Manager for a hospital system located in a great city 
in the Pacific NW.  The manager would oversee three supervisors who manage 
Histo, Cyto and Pathology Transcription.  There are about 60 Full Time 
employees working under these Supervisors. 

The position of Pathology Manager is one primarily focused on delivering 
pathology related service commitments to six hospitals and numerous outreach 
customers  by coordinating the technical and support sections of cytopathology 
processing and screening, histology, surgical pathology grossing and 
transcription with the professional physician staff (pathologists).

For more details contact me at 574.633.1231.

Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com
j...@opssearchgroup.com
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY
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Re: [Histonet] Licensing?

[Rene J Buesa]

The best thing for you to do is to visit the Florida society for 
histotechnology website. Google the name.
René J.

--- On Tue, 10/6/09, Komal Gada  wrote:


From: Komal Gada 
Subject: [Histonet] Licensing?
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 6, 2009, 9:17 PM


Hello all,
I am an ASCP certified Histotechnician (HT), i also have NY state
license.Can anyone suggest the best way to obtain a Florida license?

Thanks,

I appreciate your replies.
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Re: [Histonet] Re: Brain frozen sections

[Anne van Binsbergen]

HA!
excellent - I ran a neuropath lab for 8 years and we ALWAYS used smears - I
used to make the smears and stain the slide - by the time the Path go to the
lab all he had to do was read them!!
...but here it is different - they insist on frozens and my techs know that
most of the time there will be frezzing artefact
I will pass the message to them and then I will run and hide!! LOL ;D
thanks
greetings from the desert
Anne

2009/10/7 Tim Wheelock 

> Hi:
>
> There is one thing you might try to make frozen brain sections more than
> acceptable.
> You can freeze the tissue in liquid nitrogen vapor (LNV) (not directly into
> liquid nitrogen), let the tissue equilibrate to cryostat temperature, and
> then cut the sections.
> The histology is almost as good as a paraffin section.
>
> This is assuming that the technique fits the time frame within which you
> must arrive at a diagnosis.
> Also we ourselves do not use frozen sections to screen brains
> diagnostically. We use paraffin sections from the formalin fixed half of the
> brain.
> We send the LNV frozen blocks to investigators who cut sections from the
> frozen blocks for their research.
>
> If you would like the details, you can contact one of the following
> individuals who do this routinely at our brain bank, to see if  this
> protocol is appropriate for your needs.
>
>
>
> George Tejada
> 617-855-2646
> gtej...@mclean.org
>
> Louis Fernandes
> 617-855-2636
> lfernan...@mclean.harvard.edu
>
>
> Good luck.
> I hope this information helps.
>
>
>
> Tim Wheelock
> Assistant Director, Neuropathology
> Instructor In Neuroanatomy
> Harvard Brain Tissue Resource Center
> 203 Mailman Research Center
> McLean Hospital
> Belmont MA 02478
> Phone: 617-855-3592
> Fax: 617-855-3199
>
>
>
> Robert Richmond wrote:
>
>> You guys tell your pathologists to listen to Dr. Hessler - he taught
>> me how to do these preparations, and the basics of interpreting them,
>> when I did a locum tenens for him at the Medical College of Georgia
>> (in Augusta) 6 years ago. I did a lot of them when I got a full time
>> job at a place that did a lot of neurosurgical pathology, a year
>> later.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Knoxville TN
>> *
>> There is nothing you can do to make brain frozen sections acceptable.
>> Your Pathologists need to learn how to read smears, or just accept
>> being wrong 50% of the time. An educated guess based on the imaging is
>> more accurate than frozen sections on intra-axial primary brain
>> tumors.
>>
>> Richard B Hessler, MD
>> Chief of Pathology
>> Erlanger Medical Center
>> Chattanooga, TN
>>
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>>
>>
>>
>
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>



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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