[Histonet] Negative controls for special stains (non-organism)?!?!

[Morken, Tim]

I am reposting this because I didn't get any responses and am hoping for some 
info from other JC-inspected labs

Tim
*
Hi all,

Our Joint Commission audit was just completed (first time for JC for me). We 
passed almost everything fine.

The one thing they came up with is that we don't use "negative controls" for 
most of the special stains - like trichrome, congo red, etc. We use negative 
controls for micro-organism stains but not for the others. In fact I've never 
heard of anyone doing that and can't find any information in books, other lab 
manuals, etc. CLIA and CAP do not require negative controls for special stains.

Has anyone had this issue with JC?

Does anyone run "negative" controls for non-organism special stains?

Frankly I'm not sure how that would be done for something like a trichrome or 
other purely tissue-element stains.

Thanks for any insight!

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA

Email: tim.mor...@ucsfmedctr.org

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Her2 fixation question

[Shirley A. Powell]

I am sending this email for a friend who is having problems posting to histonet 
right now.  Please reply to her at 
carol.fie...@northside.com and not me.

>From Carol Fields
She writes:


Hi Netters,

I am attempting to write a processing schedule (VIP) for  breast bx's and 
breast tx for Her2 fixation.  We send the blocks out for testing but I cannot 
get my brain around this schedule for the processing piece.  The lab is 24 
hours with only one person from 7pm until 10 pm.  We work weekends also.  Would 
someone please let me know how they schedule the fixation and processing of 
these specimens.
Any help with this is greatly appreciated.
Thank you,
Carole



Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] EMA

[Connelly, John]

I saw the earlier post on the E29 clone and the problem with meningiomas.  We 
use the same clone and I am not too happy with it either.   Does anyone have an 
alternative clone they are happy with.

Thanks,
John Connelly


" Hello Histonetters,



We are in the market for a nicely robust EMA that will readily stain 
meningiomas.  We are currently using clone E29 but the formulation requires 
extremely long HIER and incubation times for meningiomas.



We use Ventana instruments but all opinions are welcome.



Thanks,



Linda A. Sebree

University of Wisconsin Hospital & Clinics IHC/ISH Laboratory

DB1-223 VAH

600 Highland Ave.

Madison, WI 53792

(608)265-6596"


+CONFIDENTIALITY NOTICE+
The information in this e-mail may be confidential and/or
privileged.   If you are not the intended recipient or an
authorized representative of the intended recipient, you
are hereby notified that any review,  dissemination or
copying of this e-mail and its attachments, if any, or the
information contained herein is prohibited. If you have
received this e-mail in error, please immediately notify
the sender by return e-mail and delete this e-mail from
your computer system. Thank you. 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] FW: Her2....HELP

[Rathborne, Toni]

We let the breast fix in a bread-loafed state for the desired amount of time 
(usually overnight). It is then sectioned and placed in cassettes. Whatever 
time the tissue is in formalin on the processor should be calculated so that it 
doesn't exceed the 48 hour CAP/ASCO guideline.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Carol
Fields
Sent: Thursday, October 29, 2009 2:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Her2HELP



From: Carol Fields 
Sent: Thursday, October 29, 2009 1:14 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Her2HELP

Hi Netters,

I am attempting to write a processing schedule (VIP) for  breast bx's
and breast tx for Her2 fixation.  We send the blocks out for testing but
I cannot get my brain around this schedule for the processing piece.
The lab is 24 hours with only one person from 7pm until 10 pm.  We work
weekends also.  Would someone please let me know how they schedule the
fixation and processing of these specimens.  
Any help with this is greatly appreciated.
Thank you,
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com





CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by 
Northside Hospital. It may contain information that is confidential, 
privileged, proprietary, or otherwise legally exempt from disclosure. If you 
are not the intended recipient, you are hereby notified that you are not 
authorized to read, print, retain, copy or disseminate this message, any part 
of it, or any attachments. If you have received this message in error, please 
delete this message and any attachments from your system without reading the 
content and notify the sender immediately of the inadvertent transmission. 
There is no intent on the part of the sender to waive any privilege.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from 
Somerset Medical Center and are intended only for the addressee.
The information contained in this message is confidential 
and may contain privileged, confidential, proprietary and/or 
trade secret information entitled to protection and/or exemption 
from disclosure under applicable law. Unauthorized forwarding, 
printing, copying, distribution, or use of such information is 
strictly prohibited and may be unlawful. If you are not the 
addressee, please promptly delete this message and notify 
the sender of the delivery error by e-mail or you may call 
Somerset Medical Center's computer Help Desk at 
908-685-2200, ext. 4050. 
-- 
Somerset Medical Center is the recipient of the 
2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades,
the nation's leading health care ratings company.
 
Visit Somerset Medical Center's Web site 
- www.somersetmedicalcenter.com - 
for news, event listings, health information and more.

Join the Discussion: 
Facebook: www.somersetmedicalcenter.com/fb
Twitter: www.twitter.com/SomersetMedCtr
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CDJ Institutions

[Carol Fields]

Also besides the Her2 policy...do any of you send CJD tissue to other
institutions to process?  We were sending ours out but they would not
provide a report back to our Pathologists.
THX again,
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by 
Northside Hospital. It may contain information that is confidential, 
privileged, proprietary, or otherwise legally exempt from disclosure. If you 
are not the intended recipient, you are hereby notified that you are not 
authorized to read, print, retain, copy or disseminate this message, any part 
of it, or any attachments. If you have received this message in error, please 
delete this message and any attachments from your system without reading the 
content and notify the sender immediately of the inadvertent transmission. 
There is no intent on the part of the sender to waive any privilege.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Transcription Benchmarks

[Rene J Buesa]

Under separate cover I am sending something about that.
René J.

--- On Thu, 10/29/09, Norm Burnham  wrote:


From: Norm Burnham 
Subject: [Histonet] Transcription Benchmarks
To: histonet-boun...@lists.utsouthwestern.edu, Histonet@lists.utsouthwestern.edu
Date: Thursday, October 29, 2009, 4:22 PM






Dear Histonetters, 



Does someone have benchmarks they would be willing to share about the
benchmarks they use for transcription?



Thank you in advance.



Norm Burnham





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Transcription Benchmarks

[Norm Burnham]

 

 

Dear Histonetters, 

 

Does someone have benchmarks they would be willing to share about the
benchmarks they use for transcription?

 

Thank you in advance.

 

Norm Burnham

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] imaging organ cultures

[gayle callis]

Alice, 

You didn't say if your confocal was using an inverted or upright microscope
setup.  If inverted, there are special round glass bottom dishes with cover
glass thicknesses, different diameters designed for viewing with an inverted
setup. They probably work for upright microscopes too. BD Bioscience had
culture plates with very small diameter wells (for inverted microscope)/
Try Mattek (just Google for website) and www.willcowells.com   There were
samples of WillCo Wells at the NSH Symposium/Convention, with both clear or
black glass .  ,  There are even special culture well plates for confocal
use with the well diameters very small.  I believe the culture wells come
from BD Bioscience, and Mattek has the glass bottom petri dishes. Using a
hard set antifade may help anchor your embryonic organ cultures, but these
dishes also come sterile so you can do the cultures in the dish itself,
followed by staining and coverslipping.  

Hope this helps,

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alice
Fleming
Sent: Wednesday, October 28, 2009 4:34 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] imaging organ cultures

Hi,
I am doing whole mount IHCs on embryonic organ cultures and imaging them on
a confocal microscope.  The IHCs are going well, but I'm having trouble
making the tissue stay in a specific position for imaging.  I have been
putting the tissue in the wells of depression slides, adding about 30uL of
Vectashield mounting medium, then coverslipping.  This works fine, except
that I have little control over the final position of the tissue-as I put
the coverslip down, the tissue often moves.
Any suggestions would be welcome!  Is there, for example, some kind of
optically neutral agarose that I could spot around the tissue before adding
the Vectashield and coverslip??  Or-other ideas??
Thanks,
Alice

Alice Fleming
Department of Human Genetics
University of California Los Angeles
Los Angeles, CA 90095
310-267-2456






IMPORTANT WARNING: This email (and any attachments) is only intended for the
use of the person or entity to which it is addressed, and may contain
information that is privileged and confidential. You, the recipient, are
obligated to maintain it in a safe, secure and confidential manner.
Unauthorized redisclosure or failure to maintain confidentiality may subject
you to federal and state penalties. If you are not the intended recipient,
please immediately notify us by return email, and delete this message from
your computer.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

__ Information from ESET Smart Security, version of virus signature
database 4552 (20091028) __

The message was checked by ESET Smart Security.

http://www.eset.com


 

__ Information from ESET Smart Security, version of virus signature
database 4552 (20091028) __

The message was checked by ESET Smart Security.

http://www.eset.com
 
 

__ Information from ESET Smart Security, version of virus signature
database 4552 (20091028) __

The message was checked by ESET Smart Security.

http://www.eset.com
 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Her2...help

[Carol Fields]

Hi Netters,

I am attempting to write a processing schedule (VIP) for  breast bx's
and breast tx for Her2 fixation.  We send the blocks out for testing but
I cannot get my brain around this schedule for the processing piece.
The lab is 24 hours with only one person from 7pm until 10 pm.  We work
weekends also.  Would someone please let me know how they schedule the
fixation and processing of these specimens.
Any help with this is greatly appreciated.
Thank you,
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com





CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by 
Northside Hospital. It may contain information that is confidential, 
privileged, proprietary, or otherwise legally exempt from disclosure. If you 
are not the intended recipient, you are hereby notified that you are not 
authorized to read, print, retain, copy or disseminate this message, any part 
of it, or any attachments. If you have received this message in error, please 
delete this message and any attachments from your system without reading the 
content and notify the sender immediately of the inadvertent transmission. 
There is no intent on the part of the sender to waive any privilege.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Her2....HELP

[Carol Fields]

Hi Netters,

I am attempting to write a processing schedule (VIP) for  breast bx's
and breast tx for Her2 fixation.  We send the blocks out for testing but
I cannot get my brain around this schedule for the processing piece.
The lab is 24 hours with only one person from 7pm until 10 pm.  We work
weekends also.  Would someone please let me know how they schedule the
fixation and processing of these specimens.
Any help with this is greatly appreciated.
Thank you,
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com





CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by 
Northside Hospital. It may contain information that is confidential, 
privileged, proprietary, or otherwise legally exempt from disclosure. If you 
are not the intended recipient, you are hereby notified that you are not 
authorized to read, print, retain, copy or disseminate this message, any part 
of it, or any attachments. If you have received this message in error, please 
delete this message and any attachments from your system without reading the 
content and notify the sender immediately of the inadvertent transmission. 
There is no intent on the part of the sender to waive any privilege.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FW: Her2....HELP

[Carol Fields]

From: Carol Fields
Sent: Thursday, October 29, 2009 1:14 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Her2HELP

Hi Netters,

I am attempting to write a processing schedule (VIP) for  breast bx's
and breast tx for Her2 fixation.  We send the blocks out for testing but
I cannot get my brain around this schedule for the processing piece.
The lab is 24 hours with only one person from 7pm until 10 pm.  We work
weekends also.  Would someone please let me know how they schedule the
fixation and processing of these specimens.
Any help with this is greatly appreciated.
Thank you,
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com





CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by 
Northside Hospital. It may contain information that is confidential, 
privileged, proprietary, or otherwise legally exempt from disclosure. If you 
are not the intended recipient, you are hereby notified that you are not 
authorized to read, print, retain, copy or disseminate this message, any part 
of it, or any attachments. If you have received this message in error, please 
delete this message and any attachments from your system without reading the 
content and notify the sender immediately of the inadvertent transmission. 
There is no intent on the part of the sender to waive any privilege.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Digital Pathology Systems

[Sheila Haas]

Can anyone recommend a digital pathology system? Our pathologists are 
interested in viewing slides from off-site locations. 
Thank you!
 
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] RE: alternative to Glucose oxidase (GLUOX) peroxidase block

[Andrea T. Hooper]

Couldn't agree with Gayle more!
 
Glucose oxidase is the best protocol for blocking endogenous peroxidase but 
isn't entirely necessary unless you are working with hematopoietic tissue or 
tissue that has a large leukocyte infiltrate. For muscle standard 0.3% 
H202 should be fine. Of course if you happen to be staining for leukocytes 
(neutrophils) in your muscle section, I would just buy the reagents and do the 
glucose oxidase afterall. It's simply the best.
 
That being said, I do muscle IHC on frozens for endothelial markers, and use 
0.3% H2O2. I do see bubbling but my sections do not come off and morphology 
looks great at the end. I use plus slides and dry my slides for several hours 
before putting at -80 deg C. This is both with PFA fixed tissue and acetone.
 
Good luck,
Andrea




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Import of staining reagents(richard allan/microm, now thermo)

[Truscott, Tom]

Hi Abijag, You can get MSDS forms from the company that explains any hazards 
associated with these reagents or any others. You can study these with your 
local distributor. These reagents are packed by RA to be safely shipped 
anywhere in this country and I would assume wouldn't need much extra to go to 
India. Tom Truscott

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of abi jag
Sent: Thursday, October 29, 2009 9:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Import of staining reagents(richard allan/microm, now 
thermo)

Hello histonetters,

This is with regard to import of Richard allan(microm, now thermo)staining 
reagents from US to India.

After facing several problems from stains manufactured by local manufacturers, 
we thought of switching over to RA stains.

The local distributor here is telling that these reagents(we are planning for 
procuring hematoxylin,eosin,bluing reagent and clarifier)are highly hazardous 
and need special packing(itself require 4000 US $).


Anybody having any sort of experience about this and having idea about how RA 
stains can be transported/used in other parts of the world.

Thanks for all your help

abijag 


  Try the new Yahoo! India Homepage. Click here. http://in.yahoo.com/trynew

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Fourth Annual International IHC Course in Boca Raton Jan 23-28, 2010

[Patsy Ruegg]

badze_2...@yahoo.com asked for meetings coming up in 2010.

 

 

FYI

Please visit
http://www.pathlearning.com/Pathology_Learning_Centers/Welcome.html for
detailed information of the program and how to register.



This meeting is in Jan 2010 and is a very good one.

 

Best regards,

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net 

www.ihcrg.org

 

  _  

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Automatic coverslippers

[Michael, Susan]

Does anyone have experience using Vectashield on an automatic coverslipper?
If so, how successful was it, and what model of coverslipper were you using?
What section thickness were you covering, number of sections per slide?

Susan Michael, HTL

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] SoCal Dako Autostainer service

[R C]

Is there a listing of Dako Bioengineering groups in the San Diego area who
service Autostainers?

I'm having a terrible time finding (local) reliable parties to:

A. Show up for appointments
B. Return phone calls
C. Initiate contracts

These are simple business practices not extreme circumstances and our
company prefers to service equipment and recycle versus purchasing every few
years. Again, I'd appreciate any leads in helping me achieve a goal.

Thank you.

Ruben C.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Digital Pathology and storage

[R C]

Hi All.

Regarding the recent advances in digital pathology have the standards for
slide and block storage been revised for both clinical and research
settings?

Some believe that once a slide is digitized physical slide storage should no
longer take place, while others believe storage times should be decreased.

Can anyone offer information?

Thank you in advance.

Ruben C.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] new antibody validations

[Akemi Allison-Tacha]

Hi Sharon,

This was discussed around 1 1/2 months ago in great detail.  Check  
the histonet archives for antibody validation.



Akemi Allison-Tacha BS, HT (ASCP) HTL
President/Director
Phoenix Lab Consulting
Tele: 408.402.5257
Cell: 408.335.9994
E-Mail: akemiat3...@yahoo.com

On Oct 29, 2009, at 10:31 AM, Campbell, Sharon wrote:


Hello Histonet!
We are going to be optimizing and validating new antibodies. What  
is the recommended number of cases for validating once we have  
optimized? I have heard 20 and also 40 cases. Also, How is a  
negative control to be optimized and validated?

Thank you.

Sharon Campbell, HTL(ASCP)CM, BSBM
Histology Supervisor
Celligent Diagnostics, LLC
101 East W.T. Harris Blvd, Suite 1212
Charlotte, NC  28262
800-524-6779 ext. 104
704-970-3304 Direct Line
scampb...@celligent.net

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Surgipath Automated Slide Printer

[Johnson, Teri]

Is anybody in histoland using the Surgipath Automated Slide Printer, Item 
#04V5000? If so, could you please let me know how it's working for you?

Thanks!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] new antibody validations

[Campbell, Sharon]

Hello Histonet!
We are going to be optimizing and validating new antibodies. What is the 
recommended number of cases for validating once we have optimized? I have heard 
20 and also 40 cases. Also, How is a negative control to be optimized and 
validated?
Thank you.

Sharon Campbell, HTL(ASCP)CM, BSBM
Histology Supervisor
Celligent Diagnostics, LLC
101 East W.T. Harris Blvd, Suite 1212
Charlotte, NC  28262
800-524-6779 ext. 104
704-970-3304 Direct Line
scampb...@celligent.net

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] AlkPhos

[Margaryan, Naira]

Dear histonetters,

I have to order AlkPhos kit to my IHC and, because I did not use it for the 
past 5-6 years, I do not know what company will best to order from.

Any suggestions are appreciated,

Naira

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Import of staining reagents(richard allan/microm, now thermo)

[Sherwood, Margaret]

We order RA Hematoxlylin 2 and Eosin-Y from Fisher.  Haven't heard anything
about additional cost, etc.

Peg  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of abi jag
Sent: Thursday, October 29, 2009 12:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Import of staining reagents(richard allan/microm,now thermo)

Hello histonetters,

This is with regard to import of Richard allan(microm, now thermo)staining
reagents from US to India.

After facing several problems from stains manufactured by local manufacturers,
we thought of switching over to RA stains.

The local distributor here is telling that these reagents(we are planning for
procuring hematoxylin,eosin,bluing reagent and clarifier)are highly hazardous
and need special packing(itself require 4000 US $).


Anybody having any sort of experience about this and having idea about how RA
stains can be transported/used in other parts of the world.

Thanks for all your help

abijag 


  Try the new Yahoo! India Homepage. Click here. http://in.yahoo.com/trynew

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Import of staining reagents(richard allan/microm, now thermo)

[abi jag]

Hello histonetters,

This is with regard to import of Richard allan(microm, now thermo)staining 
reagents from US to India.

After facing several problems from stains manufactured by local manufacturers, 
we thought of switching over to RA stains.

The local distributor here is telling that these reagents(we are planning for 
procuring hematoxylin,eosin,bluing reagent and clarifier)are highly hazardous 
and need special packing(itself require 4000 US $).


Anybody having any sort of experience about this and having idea about how RA 
stains can be transported/used in other parts of the world.

Thanks for all your help

abijag 


  Try the new Yahoo! India Homepage. Click here. http://in.yahoo.com/trynew

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Tissue-Tek II Manual

[Yang, YangMing]

Hello,

I saw your email from internet. We have an old Cryostat (model: Tissue-Tek II 
4553 from Miles) without the instruction manual. It will be very grateful if 
you already found one, and can shear with us. If you have it could you email me 
a copy? We are trying to fix our Cryostat.

Best regards,

Yang-Ming Yang
Dept of Physiology
New York Medical College
BSB, room 629

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Celerus Wave

[O'Donnell, Bill]

Good day,
 
Is anyuone using the Celerus Wave rapid IHC system? Would appriciate any
feedback.
 
William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RELIA Histology Job Alert for Supervisors, Managers Techs and PAs. Hot Hot Hot Jobs!!

[Pam Barker]

Hi Histonetters!!
I hope everyone is having a great day and ramping up for a great
weekend.  Any plans for Halloween?  
I have some great opportunities that I want to tell you about.  All of
these opportunities are permanent full time positions with some of my
best clients who are offering excellent compensation and benefits and in
most cases relocation and or sign on bonuses and the newest and hottest
positions have an *
Histology Management
Histology Manager - Charlotte, NC*
Pathology Supervisor - Fresno, CA*
Histology Supervisor - Los Angeles, CA
Pathology Supervisor - Cape Cod, MA
Pathology Manager - Portland, OR
Histology Supervisor - Austin, TX*
Histology Supervisor - Spokane, WA
 
Pathology Assistant
PA - Charlotte NC*
 
Histotechnicians/Histotechnologists
Lead Histotech - Tucson area, AZ*
Grossing Histotech - Atlanta, GA (night shift)*
Histotech - Pittsburgh
Histotech - Orange/Rockland, Cty, NY
Histotech - Plattsburgh, NY
Histotech - Largo, FL part time afternoons M-F
 
If you or anyone you know might be interested in hearing more about any
of these opportunities please contact me.  I can be reached at
rel...@earthlink.net or toll free at 866-607-3542.

Thank You!
 
Pam Barker
President
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
Toll Free: (866)607-3542
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
<>
www.myspace.com/pamatrelia 
www.twitter.com/pamatrelia

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Canine endothelial antibody

[Jan Shivers]

Jackie,

Dako's Factor VIII (von Willebrand Factor), cat. #A0082 works for me on 
canine tissue (and cat, pig, cow, horse, sheep, chicken, mouse, etc.).  I 
use Proteinase K enzyme digestion pretreatment for 5' at room temperature; 
primary rabbit Ab incubation 30', EnVision+/HRP anti-Rabbit 30' (with 4% 
normal dog serum added per volume for blocking), AEC 10'.


Hope this helps,

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)



- Original Message - 
From: "Jackie M O'Connor" 
To: ; 


Sent: Wednesday, October 28, 2009 3:35 PM
Subject: [Histonet] Canine endothelial antibody



A week or so ago I thought I saw a post about an antibody that works for
canine endothelial cells, but I can't find it in the archives - was I just
dreaming, because I need this so bad?   I've tried Factor VIII and a CD34
on FFPE, but with poor results.   Any help would be most appreciated.
Thanks,
Jackie O'

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] GFAP IHC

[Jan Shivers]

Nancy,

I don't use the ready-to-use GFAP from Dako, but I do use the concentrated 
form of GFAP (it is also a rabbit anti-cow GFAP.)  It's worked successfully 
in dog, cat, pig, cow, horse, goat, sheep, deer, eagle, mouse, rat, gerbil, 
guinea pig, hamster, monkey, ferret, and kangaroo with no background 
problems whatsoever.


The EnVision+HRP Rabbit will only bind to the rabbit IgG, not the cow IgG, 
so that is not the source of your background.  I would suspect that if 
you're getting brown staining on the slide itself, your antibody is much 
much too concentrated.  My working concentration calculates out to be 4 
ug/ml of Ab.  You might see if your ready-to-use preparation has a 
concentration listed, and then work from there to approximate a 4 ug/ml 
concentration with diluting it out.


If that doesn't work... buy the concentrated stock form and optimize it for 
your tissues.  In my lab, it requires absolutely no tissue pretreatment, and 
the stain is always strong, clear and clean.  Good Luck!


Best regards,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)




- Original Message - 
From: "Nancy Herman" 

To: 
Sent: Wednesday, October 28, 2009 3:19 PM
Subject: [Histonet] GFAP IHC


We are using DAKO's ready-to-use GFAP antibody on our bovine tissue and 
using Envision+ HRP Rabbit detection system.  When using this antibody we 
see a brown color on the slide itself.  The staining was intense and quite 
uneven.  We have diluted the anitbody to 1:10 but there is still the brown 
slide.  The staining is not quite as uneven but appears more washed out. 
We don't see this with any of our other antibodies we are using and our no 
primary controls for GFAP are clean.  Any advice or explanations for the 
slides picking up the brown color or improving GFAP IHC on bovine tissue?

Thanks

Nancy Herman
CFIA Lethbridge Laboratory
Lethbridge, Alberta







___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Canine endothelial antibody

[Amy Porter]

We use Dako Von Willebrand Factor VIII in Rabbit on multiple species and it 
works fabulously.  Enzyme pretreatment with 0.03% pronase e for 10 mins at 
37C / Primary at 1:200 to 1:500 depending on the species being stained.

Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  port...@msu.edu
Web:  www.humanpathology.msu.edu
- Original Message - 
From: "Jackie M O'Connor" 
To: ; 


Sent: Wednesday, October 28, 2009 4:35 PM
Subject: [Histonet] Canine endothelial antibody



A week or so ago I thought I saw a post about an antibody that works for
canine endothelial cells, but I can't find it in the archives - was I just
dreaming, because I need this so bad?   I've tried Factor VIII and a CD34
on FFPE, but with poor results.   Any help would be most appreciated.
Thanks,
Jackie O'

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] GFAP IHC

[Amy Porter]

If I remember correctly Dako's GFAP is a bovine immunogen (bovine spinal 
cord) - that is probably what is causing the problem.  You may want to look 
for GFAP where the cow is not the immunogen / try running another brain from 
a different species and see if you get the same problem.


Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  port...@msu.edu
Web:  www.humanpathology.msu.edu
- Original Message - 
From: "Nancy Herman" 

To: 
Sent: Wednesday, October 28, 2009 4:19 PM
Subject: [Histonet] GFAP IHC


We are using DAKO's ready-to-use GFAP antibody on our bovine tissue and 
using Envision+ HRP Rabbit detection system.  When using this antibody we 
see a brown color on the slide itself.  The staining was intense and quite 
uneven.  We have diluted the anitbody to 1:10 but there is still the brown 
slide.  The staining is not quite as uneven but appears more washed out. 
We don't see this with any of our other antibodies we are using and our no 
primary controls for GFAP are clean.  Any advice or explanations for the 
slides picking up the brown color or improving GFAP IHC on bovine tissue?

Thanks

Nancy Herman
CFIA Lethbridge Laboratory
Lethbridge, Alberta







___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet