[Histonet] out of office

[Marilyn . A . Weiss]

I will be out of the office starting  11/23/2009 and will not return until
11/30/2009.

.In my absence please ask for Mary Campbell .  If this is urgent you can
contact me on my cell phone number 858-472-4266.
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Re: [Histonet] 4 HISTOTECH POSITIONS IN kAISER

[Kiranjit Grewal]

Kaiser Regional Laboratory in Berkeley, CA has 4 full time Histotech openings, 
if interested please call Kiranjit @ 510-559-5404.
 


--- On Mon, 11/16/09, Isaac O  wrote:


From: Isaac O 
Subject: [Histonet] NEW POSITION WANTED PLS
To: histonet@lists.utsouthwestern.edu
Date: Monday, November 16, 2009, 12:22 PM



  Hi,
   I am looking for a new HISTOTECH/IHC position. I am  both HT(ASCP) and 
HTL(ASCP) certified. I am open to relocation most especially , the MIDWEST, 
NORTH EAST, SOUTH EAST and the NORTH WEST.
   I also have some management experience.

 Isaac.



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[Histonet] need old processing baskets

[Patricia F Lott]

Help!  I obtained an old Tissue-Tek II 4640 tissue processor, but I need the 
baskets, and a manual, if possible!

Patty Lott, Laboratory Manager
UAB CMBD Core Laboratory 205-934-2007
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RE: [Histonet] TNF alpha

[Liz Chlipala]

Sorry Guys I was thinking of IL-6 which I have done on bone and it
works.  We have worked with TNF-alpha (from Sigma) and our protocol does
not requite retrieval I would think it would work on formic acid
decalcified bone also, but I have not tried it.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, November 23, 2009 3:25 PM
To: Histonet
Subject: [Histonet] TNF alpha

I have a colleague who is interested in doing IHC for TNF alpha on
formalin-fixed, decalcified bone specimens.  Is this possible?  If so, I
would appreciate hearing about your antibody and technique.  Thanks!

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax


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[Histonet] Open Position

[Joseph Saby]

I have a full time position open for a histotech at NAMSA, located just south 
and east of Toledo in Northwood, Ohio.  This is a full time days position, and 
requires skills in embedding, sectioning, staining, etc.  Must be willing to 
work on animal tissues.  We work on many interesting specimens, and are always 
working to expand our capabilities.

NAMSA is a CRO that performs a great deal of medical device testing.  Please 
feel free to look us up on the internet.  If you have any questions, you can 
respond to me at this email address, or at js...@namsa.com.

Thanks!

Joe Saby, BA HT(ASCP)



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RE: [Histonet] TNF alpha

[Liz Chlipala]

Richard it works.  We purchase the antibody from Abcam.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, November 23, 2009 3:25 PM
To: Histonet
Subject: [Histonet] TNF alpha

I have a colleague who is interested in doing IHC for TNF alpha on
formalin-fixed, decalcified bone specimens.  Is this possible?  If so, I
would appreciate hearing about your antibody and technique.  Thanks!

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax


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[Histonet] TNF alpha

[Richard Cartun]

I have a colleague who is interested in doing IHC for TNF alpha on 
formalin-fixed, decalcified bone specimens.  Is this possible?  If so, I would 
appreciate hearing about your antibody and technique.  Thanks!

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax


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RE: [Histonet] Mouse eyes

[gayle callis]

Davidsons fixative is popular for eyes.  This has been discussed at great
length on Histonet, so search the Archives.  I believe it works for IHC.
Gluteraldehyde can crosslink antigens too strongly if IHC is needed.  Retina
commonly detaches, at least with larger eyes we have worked with, when the
eye section is flattened on a water bath.  The water bath temperature used
was a few degrees lower than normally used for other tissues. You should
drain the slides well in upright position if using Plus charge.  Lay flat on
slide warmer, to dry at 37C to 40C overnight or longer as we do for
decalcified bone sections.

There are other ways to flatten eyes by floating an eye section on 5%
alcohol water bath, then picking up on a slide, and slowly lowering the
section (keep top part of paraffin of section attached to slide, and
lowering this into a warm waterbath.  The surface tension is reduced by the
alcohol, allowing the eye to very slowly flatten, then pick up, drain well
then dry flat. This takes a bit of practice but also works for decalcified
bone when the softer cartilage likes to wrinkle versus the harder bone.
Always pays to slow down speed of water rinsing, if the water flows too
hard, during staining.  

Gayle Callis
HTL/HT/MT(ASCP) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Moretz
Sent: Monday, November 23, 2009 2:15 PM
To: Durden, Kelley; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Mouse eyes

Altho' retired for 2 years, we had a protocol that retained all parts of the
eye quite well.  Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C
for overnight.  Don't extend the fixation as this will cause the tissue to
be too hard.  Wash for about 1hr in running tap water and process as usual
for mouse tissue (if it works for mouse liver, it will work for the eyes).  

Roger Moretz, Ph.D. (ret.)



- Original Message 
From: "Durden, Kelley" 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Mon, November 23, 2009 10:44:21 AM
Subject: [Histonet] Mouse eyes

My question concerns mouse eyes.

Can anyone send me their fixation suggestions?  We are receiving mouse eyes
that end up with a "wavy" look instead of a nicely defined "C" and have no
explanation for this occurrence.

Can anyone send me their mouse eye processing schedule?  We have a protocol
that has proved fast and true but would welcome other suggestions.

Can you give me a good idea for making sure sections stick to slides well? 
Gold Plus?  What else?

Has anyone experienced a retinal detachment after or upon staining?  Retina
attached after sectioning - detached after staining - just routine H&E. 
Should I heat for an extended period of time?

Kelley Durden, HT ASCP
University of Florida
Molecular Pathology Core
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Re: [Histonet] Mouse eyes

[Roger Moretz]

Altho' retired for 2 years, we had a protocol that retained all parts of the 
eye quite well.  Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C 
for overnight.  Don't extend the fixation as this will cause the tissue to be 
too hard.  Wash for about 1hr in running tap water and process as usual for 
mouse tissue (if it works for mouse liver, it will work for the eyes).  

Roger Moretz, Ph.D. (ret.)



- Original Message 
From: "Durden, Kelley" 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Mon, November 23, 2009 10:44:21 AM
Subject: [Histonet] Mouse eyes

My question concerns mouse eyes.

Can anyone send me their fixation suggestions?  We are receiving mouse eyes 
that end up with a "wavy" look instead of a nicely defined "C" and have no 
explanation for this occurrence.

Can anyone send me their mouse eye processing schedule?  We have a protocol 
that has proved fast and true but would welcome other suggestions.

Can you give me a good idea for making sure sections stick to slides well?  
Gold Plus?  What else?

Has anyone experienced a retinal detachment after or upon staining?  Retina 
attached after sectioning - detached after staining - just routine H&E.  Should 
I heat for an extended period of time?

Kelley Durden, HT ASCP
University of Florida
Molecular Pathology Core
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[Histonet] job opening at UCLA

[Linke, Noelle]

Hi all,

I have an opening for a Histotech III in the histology lab which (it was just 
posted).  For this position, an HTL is required, no exceptions.  We are looking 
for someone with 5+ years of experience in routine histology, manual specials, 
automated special stain experience is always a help, as well as someone with 
leadership skills.

To apply, enter the job number H51527 in the 'Find Job Code box.
https://jobs2.mednet.ucla.edu/css_external/CSSPage_BrowseJobs.ASP

Salary range for this position is $36.83-$47.66 per hour DOE.

If you are interested, please let me know!

Thanks,
Noelle

Noёlle Linke M.S., HTL(ASCP)QIHC
Manager, Histology Services
Department of Pathology & Laboratory Medicine
David Geffen School of Medicine at UCLA
Phone: 310-825-7397
Pager: 97471
nli...@mednet.ucla.edu







  
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Unauthorized redisclosure or failure to maintain confidentiality may subject 
you to federal and state penalties. If you are not the intended recipient, 
please immediately notify us by return email, and delete this message from your 
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[Histonet] RE: Histonet Digest, Vol 72, Issue 26

[Suresch, Donna L.]

RE:  Cryojane Tape Transfer System
I have found that you need to have your tape at the same temp as your
tissue to have your tissue transfer completely from the tape.  I set
everything up in the morning putting the tape in the cryostat and get
everything equilibrated first for about 2 hours or so.  
Hope it helps. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Monday, November 23, 2009 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 72, Issue 26

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. HT Opening (Marian Powers)
   2. cryojane tap transfer system (Richard Pattison)
   3. Re: cryojane tap transfer system (Merced M Leiker)
   4. Suggestions for Cassette Labeling (Fye Beth)
   5. RE: Suggestions for Cassette Labeling (Rathborne, Toni)
   6. Reference labs doing parafibromin? (Sebree Linda A)
   7. Mouse eyes (Durden, Kelley)
   8. Job Opening (Walzer Susan)
   9. Reponses to Merced and Richard Re: [Histonet] cryojane tape
  transfer  system  (gayle callis)
  10. RE: cryojane tap transfer system (CHRISTIE GOWAN)


--

Message: 1
Date: Sun, 22 Nov 2009 13:54:28 -0500
From: Marian Powers 
Subject: [Histonet] HT Opening
To: histonet@lists.utsouthwestern.edu
Message-ID:
<5d7de0e60911221054i5971a474y64a461eeb5aa8...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

DPS in Dover, Delaware, is currently seeking a full time histotech, day
or
evening shift.  All inquiries please email mpo...@dpspa.com



-- 
Marian L. Powers, HT(ASCP)
Manager, Technical Operations

Doctors Pathology Services
1253 College Park Drive
Dover, DE 19904
302-677-


--

Message: 2
Date: Mon, 23 Nov 2009 09:01:39 -0500
From: Richard Pattison 
Subject: [Histonet] cryojane tap transfer system
To: Histonet@lists.utsouthwestern.edu
Message-ID: <4b0a95c3.4090...@googlemail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi Everybody,
I was hoping to get some advice - I'm cryosectioning plant tissues and 
transferring sections to slides using the Cryojane system. However, i'm 
having problems in transferring the sections without them falling apart 
during the tape transfer. I'm fixing my tissue for 24 hours in 
ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then 
sectioning at between 2 and 14 microns. The sections seem to be ok but 
whenever i remove the adhesive tape from the slide a large part of the 
tissue is removed with it. As a result I lose the majority of my 
section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides 
from instrumedics) but neither have given satisfactory results.
Does anyone have any suggestions as to how i could reduce the loss of 
tissue? Any advice would be much appreciated.
Thanks
Richard



--

Message: 3
Date: Mon, 23 Nov 2009 09:26:51 -0500
From: Merced M Leiker 
Subject: Re: [Histonet] cryojane tap transfer system
To: Richard Pattison ,
Histonet@lists.utsouthwestern.edu
Message-ID: <5d6d90dc816b8e32d70f0...@cdywxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed

Unless I'm missing something, I don't understand why people use this
tape? 
It seems like a marketing gimmick to me...ol' fashion' melting of
sections 
onto slides works perfectly for us...

?

Regards,
Merced

--On Monday, November 23, 2009 9:01 AM -0500 Richard Pattison 
 wrote:

> Hi Everybody,
> I was hoping to get some advice - I'm cryosectioning plant tissues and
> transferring sections to slides using the Cryojane system. However,
i'm
> having problems in transferring the sections without them falling
apart
> during the tape transfer. I'm fixing my tissue for 24 hours in
> ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then
> sectioning at between 2 and 14 microns. The sections seem to be ok but
> whenever i remove the adhesive tape from the slide a large part of the
> tissue is removed with it. As a result I lose the majority of my
section.
> I've tried using both 1x and 1/2x slides (CFSA adhesive slides from
> instrumedics) but neither have given satisfactory results.
> Does anyone have any suggestions as to how i could reduce the loss of
> tissue? Any advice would be much appreciated.
> Thanks
> Richard
>
> 

Re: [Histonet] cryojane tap transfer system

[Nicole Collette]

Hello, Richard,

I have done some sectioning of undecalcified bone with the Cryojane 
(hallelujah! it works! to paraphrase Ms. Callis) and one thing is 
that the sections won't cross-link if the tapes or the slides are 
being stored in the presence of UV light (then they are 
pre-crosslinked!), keep them dark and dry when not using, and don't 
take them into and out of the cryostat, only bring in what you need. 
I have also found that thicker sections don't crosslink as well, I've 
had trouble with sections over 6um (although I am far from am expert 
at cryosectioning). Temperature is also somewhat of a factor, I've 
found that I would section at colder temperature than I normally 
would for that tissue to get it to crosslink well- sounds 
counterintuitive, but there it is. This is purely based on my own 
trial and error, take it with a grain of salt and good luck.


Sincerely,
Nicole Collette
LLNL/UC Berkeley


At 9:01 AM -0500 11/23/09, Richard Pattison wrote:

Hi Everybody,
I was hoping to get some advice - I'm cryosectioning plant tissues 
and transferring sections to slides using the Cryojane system. 
However, i'm having problems in transferring the sections without 
them falling apart during the tape transfer. I'm fixing my tissue 
for 24 hours in ethanol:acetic acid (3:1), embedding in O.C.T, 
snap-freezing and then sectioning at between 2 and 14 microns. The 
sections seem to be ok but whenever i remove the adhesive tape from 
the slide a large part of the tissue is removed with it. As a result 
I lose the majority of my section. I've tried using both 1x and 1/2x 
slides (CFSA adhesive slides from instrumedics) but neither have 
given satisfactory results.
Does anyone have any suggestions as to how i could reduce the loss 
of tissue? Any advice would be much appreciated.

Thanks
Richard

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Reponses to Merced and Richard Re: [Histonet] cryojane tape transfer system

[gayle callis]

You wrote:

 

Unless I'm missing something, I don't understand why people use this tape? 

It seems like a marketing gimmick to me...ol' fashion' melting of sections 

onto slides works perfectly for us...

?

Regards,

Merced

 

Merced, 

Yes,  you are missing something.  If you have ever tried to cryosection
undecalcified bone or extremely difficult tissues that simply will not
result in "ol' fashion' melting" onto a slide , then you would understand
why people use this unique cryosectioning system.  It is not some
"marketing  gimmick"  but an unique instrument  helping many laboratories
obtain frozen sections that otherwise are scrunched up,  shattered, and
destroyed.  I suggest you go to the Instrumedics website or www.alphelys.com
for a superb slide show  and learn how this instrument works before making
assumptions about a technology that serves many of us more than well.  

 

A happy, informed user of the Cryojane

 

Gayle M. Callis

HTL/HT/MT(ASCP)

Bozeman MT 

 

As for what Richard wrote: 

 

Hi Everybody,
I was hoping to get some advice - I'm cryosectioning plant tissues and 
transferring sections to slides using the Cryojane system. However, i'm 
having problems in transferring the sections without them falling apart 
during the tape transfer. I'm fixing my tissue for 24 hours in 
ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then 
sectioning at between 2 and 14 microns. The sections seem to be ok but 
whenever i remove the adhesive tape from the slide a large part of the 
tissue is removed with it. As a result I lose the majority of my 
section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides 
from instrumedics) but neither have given satisfactory results.
Does anyone have any suggestions as to how i could reduce the loss of 
tissue? Any advice would be much appreciated.
Thanks
Richard
 
Dear Richard, 
 
I could be the fixative you are using that causes the problem.   If the
fixative contains alcohol, the alcohol acts as antifreeze when you try to
snap freeze a tissue, animal or plant.  The alcohol may cause problems with
how the pink tape sticks to the face of the plant tissue, and allows them to
fall apart during the tape transfer.   If you rinse away the fixative, then
you should cryoprotect the fixed plant tissue with 30% sucrose before snap
freezing. vbThis will remove the alcohol.  If cryoprotection causes problems
with the final staining results, then try unfixed plant tissue, snap freeze,
Cryojane tape transfer the section and then fix the transferred plant
section in  your favorite fixative.   You may have to optimize the time in
fixative though.   
 
Other suggestions:  
 
Do a double UV flash, but wait for 15 to 20 seconds between flashes.  You
must allow the UV light source (capacitor) build up enough charge to work
properly.  This double flash seems to help polymerize the coating more
thoroughly, and the section should transfer better.  Also, the tape must be
removed at an angle across the slide,  very slowly, and inside the cryostat
(I am sure you probably do this already.) 
 
Also, try the 4X slide if you still have problems  with 1/2X and 1X slides.
You might ask Leica to send you a few to try before investing in a whole box
of these.  Contact Emmanuel Mineo, Intrumedics Product Manager
emmanuel.mi...@leica-microsystems.com for the 4X slides.  Manny is a nice
gentleman who has worked with Cryojane for many years and has always been
helpful to us.   Once again, do the double UV light flash with the 4X
slides. They are gooey, but may/should hold more securely.  
 
With undecalcified bone, we use the 1/2X but do the double flash routinely
for all sections.   
 
Good luck
 
Gayle Callis
 
 
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RE: [Histonet] cryojane tap transfer system

[CHRISTIE GOWAN]

 Hi Richard,

I am assuming that you are using the flash in the cryojane system to adhere the 
specimen to the slide. Make sure your tape is not too old and don't store it in 
the cryostat. It sounds like you are able to get the section but unable to get 
it to adhere. Make sure you pass your roller over the tape and slide several 
times before putting it under the flash and always use a positive charged slide 
from a freshly opened box. Good luck. I hope this helps a little

Christie
  
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[Histonet] Job Opening

[Walzer Susan]

We have an opening for a tech, days no weekends at St Pete.General Hospital in  
St Pete,FL.  Call our HR dept at 727 384-1414 X 4814.
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[Histonet] Mouse eyes

[Durden, Kelley]

My question concerns mouse eyes.

Can anyone send me their fixation suggestions?  We are receiving mouse eyes 
that end up with a "wavy" look instead of a nicely defined "C" and have no 
explanation for this occurrence.

Can anyone send me their mouse eye processing schedule?  We have a protocol 
that has proved fast and true but would welcome other suggestions.

Can you give me a good idea for making sure sections stick to slides well?   
Gold Plus?  What else?

Has anyone experienced a retinal detachment after or upon staining?  Retina 
attached after sectioning - detached after staining - just routine H&E.  Should 
I heat for an extended period of time?

Kelley Durden, HT ASCP
University of Florida
Molecular Pathology Core
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[Histonet] Reference labs doing parafibromin?

[Sebree Linda A]

Good Monday morning,

One of our pathologists is getting pressure from the endocrinologists to
bring on Parafibromin (gene: hrpt2).  Are there any reference labs out
there offering this?

Thanks,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


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RE: [Histonet] Suggestions for Cassette Labeling

[Rathborne, Toni]

That seems like a lot of effort. Do your pathologists do the grossing? or PA's? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Fye Beth
Sent: Monday, November 23, 2009 9:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Suggestions for Cassette Labeling


There have been some great suggestions for reducing or catching slide labeling 
errors.  I'm interested in the same for cassette labeling.  Currently, we have 
the patient's last name on one side of the cassette, and the site listed on the 
other.  This makes it easier to identify blocks if they are numbered 
incorrectly.

Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638





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[Histonet] Suggestions for Cassette Labeling

[Fye Beth]

There have been some great suggestions for reducing or catching slide labeling 
errors.  I'm interested in the same for cassette labeling.  Currently, we have 
the patient's last name on one side of the cassette, and the site listed on the 
other.  This makes it easier to identify blocks if they are numbered 
incorrectly.

Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638





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Re: [Histonet] cryojane tap transfer system

[Merced M Leiker]

Unless I'm missing something, I don't understand why people use this tape? 
It seems like a marketing gimmick to me...ol' fashion' melting of sections 
onto slides works perfectly for us...


?

Regards,
Merced

--On Monday, November 23, 2009 9:01 AM -0500 Richard Pattison 
 wrote:



Hi Everybody,
I was hoping to get some advice - I'm cryosectioning plant tissues and
transferring sections to slides using the Cryojane system. However, i'm
having problems in transferring the sections without them falling apart
during the tape transfer. I'm fixing my tissue for 24 hours in
ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then
sectioning at between 2 and 14 microns. The sections seem to be ok but
whenever i remove the adhesive tape from the slide a large part of the
tissue is removed with it. As a result I lose the majority of my section.
I've tried using both 1x and 1/2x slides (CFSA adhesive slides from
instrumedics) but neither have given satisfactory results.
Does anyone have any suggestions as to how i could reduce the loss of
tissue? Any advice would be much appreciated.
Thanks
Richard

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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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[Histonet] cryojane tap transfer system

[Richard Pattison]

Hi Everybody,
I was hoping to get some advice - I'm cryosectioning plant tissues and 
transferring sections to slides using the Cryojane system. However, i'm 
having problems in transferring the sections without them falling apart 
during the tape transfer. I'm fixing my tissue for 24 hours in 
ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then 
sectioning at between 2 and 14 microns. The sections seem to be ok but 
whenever i remove the adhesive tape from the slide a large part of the 
tissue is removed with it. As a result I lose the majority of my 
section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides 
from instrumedics) but neither have given satisfactory results.
Does anyone have any suggestions as to how i could reduce the loss of 
tissue? Any advice would be much appreciated.

Thanks
Richard

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