[Histonet] To my fellow histotechs....

[Colleen Forster]

I am gearing up for a new project and have a list of primaries. I would 
appreciate any help on what others are using for these. The work will be 
done on human samples.


   * Cyclin D1
   * Cyclin D2
   * Cyclin D3
   * CD38
   * CD56
   * CD138
   * Kappa
   * Lambda
   * BLIMP-1
   * XBP-1
   * MUM-1

Thanks in advance...

Colleen Forster HT(ASCP)QIHC
Bionet Histology Service
U of MN
612-626-1930
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[Histonet] Vimentin for Mouse

[Bell, Pat]

Thank you all very much for your help regarding the COX2 for the mouse. Now I 
would like to ask your help regarding Vimentin for FFPE mouse tissue. I have 
tried Dako and Sigma with no success. I am also not sure of what clone to use.

Thanks again.
Pat


Pat Bell HT(ASCP)
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu


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RE: [Histonet] Staffing for IHC

[Thomas Jasper]

I would totally agree Angela.  Without knowing all the details, I'd bet
these people are plenty busy all day everyday.  I'd worry about burning
them out.  And what happens when one or the other is sick or needs a
vacation?

Tom J

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, December 17, 2009 9:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Staffing for IHC

I'm hoping some of the larger hospitals out there will share their data
with me.
I'm curious as to how labs are staffed for doing IF/IHC/CISH. 
A small portion of our staining protocols still require pretreatment by
hand, but we run instruments that do the pretreatments on board for the
majority of our stains.
We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are
cutting and staining between 200-350 slides per day. I think this is a
high volume for only two people. What do you all think?



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taken, in reliance on it is prohibited and may be unlawful. If you have
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[Histonet] Re: Histonet Digest, Vol 73, Issue 26

[Stephanie Rodriguez]

Hi Linda,

We routinely run this antibody, and would be happy to perform it for you.
Feel free to contact our Client Services department at the phone number
below regarding specimen submission.

Stephanie Rodriguez, HTL(ASCP), QIHC
IHC Tech III
Phenopath Laboratories
551 N. 34th St  Ste 100
Seattle, WA  98103
(206) 374-9000

> 
> Message: 8
> Date: Thu, 17 Dec 2009 09:13:40 -0600
> From: "Sebree Linda A" 
> Subject: [Histonet] hsNFS(INI1) antibody
> To: "Histonet" 
> Message-ID:
>  <8c023b4ab999614ba4791baeb26e27381bf...@uwhc-mail01.uwhis.hosp.wisc.edu>
>  
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi,
> 
> I'm looking for a reference lab that performs an IHC stain for this
> antigen.  Its used for diagnosing malignant rhabdoid tumor where this
> antigen is lost.  It is expressed in normal tissue.
> 
> Thanks,
> 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> 
> 
> 



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original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
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RE: [Histonet] Silver Nitrate instead of inking??

[Nancy Klemme]

Dear Sheila,

I would strongly recommend that you obtain approval from the manufacturer of 
the tissue processors into which you will be putting these tissues before you 
would begin this practice.  The negative accumulative affect on several of the 
internal components of the instrument can (and probably will) eventually cause 
the instrument to fail.

You would see the effect of the silver nitrate on any metal products it would 
come into contact with: forceps, metal holding container for the racks or 
baskets, the racks or baskets themselves if they are metal.

It is not approved for use on any model of Tissue Tek Tissue Processors.

Many years ago this practice was tolerated by the old tissue-transfer 
processors.  Some people even used this inking method and placed tissues on 
earlier models of the fluid-transfer processors.  Service that was required in 
those years did not include investigation as to what was placed into the 
instrument (not simply the reagents, but what also came in on the specimens) 
that could have been the root cause of the problem.

I hope this informative and that you continue to use your current inking 
product or investigate others that are safer for you and your processors.

With kind regards and wonderful holiday wishes,
Nancy Klemme
EduSvcsDir - Sakura Finetek

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sheila adey
Sent: Thursday, December 17, 2009 3:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Silver Nitrate instead of inking??



Hi Everyone,



Has anyone ever heard of using Silver Nitrate for inking skin bx's before 
processing???

One of our paths mentioned it.



Thanks in advance.


Sheila Adey HT MLT



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Re: [Histonet] Staffing for IHC

[Rene J Buesa]

The range for histotechs "doing other tasks" is from 200 to 78,300 slides/year 
(1 to 301 slides/day).
The threshold from 99 laboratories is 9,500 slides/year = 37 slides/day per 
histotech.
René J.

--- On Thu, 12/17/09, Angela Bitting  wrote:


From: Angela Bitting 
Subject: [Histonet] Staffing for IHC
To: histonet@lists.utsouthwestern.edu
Date: Thursday, December 17, 2009, 12:42 PM


I'm hoping some of the larger hospitals out there will share their data with me.
I'm curious as to how labs are staffed for doing IF/IHC/CISH. 
A small portion of our staining protocols still require pretreatment by hand, 
but we run instruments that do the pretreatments on board for the majority of 
our stains.
We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are 
cutting and staining between 200-350 slides per day. I think this is a high 
volume for only two people. What do you all think?



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to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
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prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
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[Histonet] Re: veterinary lab question - chem expiration

[Silvina Molinuevo]

Hi Jan! with the lote number you can write to the manufacturer and they will 
inform you expiration times.
Best wishes, sil




  Yahoo! Cocina

Encontra las mejores recetas con Yahoo! Cocina.


http://ar.mujer.yahoo.com/cocina/
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[Histonet] RE: immunoperoxidase background staining

[Troutman, Kenneth A]

A couple of suggestions.  First, what kind of fixative are you using?  If you 
are using a heavy metal fixative, sometimes that can be the reason.  We have 
had this issue with B-plus fixative (B5 replacement) and I have seen it with 
some protocols in zinc formalin and non-buffered fixatives.

Second, try backing off of the CC1 or 2.  I use a Biocare's Background 
Terminator in an option container to help with my most troublesome antibodies.  
You can also use the green diluent they provide you with as a blocking reagent. 
 I found it works on most things.  If you are having issues with tons of 
background on ALL your slides, I would send a slide to a place that has 
established protocols to see if it is the instrument or the tissue.

Hope this helps.

Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN  37232
615-343-9134
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[Histonet] histotechs grossing

[Marilyn . A . Weiss]

Can you please tell me if there are any HT's  or HTL's grossing complex 
cases.  If so , are they trained by their Pathologist or a school?  Please 
e-mail me direct. Thank you.
marilyn.a.we...@kp.org.

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[Histonet] Can anyone help me?

[Mike Valerio]

Hello,
I would like to stain FFPE mouse brain tissue with MCP-1 antibody from serotec. 
I was wondering 
if anyone who uses this marker could share their protocol and indicate what is 
a good positive 
control. Thanks in advance.
Michael Valerio
University at Buffalo
valer...@buffalo.edu
716-829-5650






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[Histonet] Re: Bubbles on Fresh Frozen tissue after IHC

[Johnson, Teri]

Hi Nick,

Do your peroxidase blocking offline in a coplin jar or staining bucket, prior 
to loading them in the capillary gap holder.

Problem solved.

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO


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RE: [Histonet] Staffing for IHC

[McMahon, Loralee A]

I have 2 full time techs (one of those techs is supposed to be devoted to 
research), one part time tech that cuts controls and one part time tech on the 
overnight shift to load the machines.   I am supposed to only be at the bench 
20% of the time, but it is much more, trying to cover vacations and sick time.  
I also help out on the research end and we have lots of it.
There are times when we are extremely overwhelmed with IHC's and ISH/CISH 
We do about 35K IHC a year, not including the research slides. 


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting 
[akbitt...@geisinger.edu]
Sent: Thursday, December 17, 2009 12:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Staffing  for IHC

I'm hoping some of the larger hospitals out there will share their data with me.
I'm curious as to how labs are staffed for doing IF/IHC/CISH.
A small portion of our staining protocols still require pretreatment by hand, 
but we run instruments that do the pretreatments on board for the majority of 
our stains.
We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are 
cutting and staining between 200-350 slides per day. I think this is a high 
volume for only two people. What do you all think?



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to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
distribution or any action taken, or omitted to be taken, in reliance on it is 
prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
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Re: [Histonet] Staffing for IHC

[Pat Laurie]

Angela,

We do about the same number of IHC slides.  We run primarily Ventana
machines (one of the main benefits are reduced tech time) and are expanding
into the intellipath.  Our IHC department is relatively large, we have
dedicated 1 person for leading and development, one person on both 1st and
2nd shift running the IHC as well as 1 person who will assist as needed on
both shifts.  Our IF and ISH are done on a seperate bench.  We still have
days where our IHC bench is overwhelmed even with as many people as we
have.  It seems that IHC volume per case in all institutions is going up as
more tests are being developed.



Patrick Laurie HT(ASCP)QIHC

Histology Supervisor

CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104




On Thu, Dec 17, 2009 at 9:42 AM, Angela Bitting wrote:

> I'm hoping some of the larger hospitals out there will share their data
> with me.
> I'm curious as to how labs are staffed for doing IF/IHC/CISH.
> A small portion of our staining protocols still require pretreatment by
> hand, but we run instruments that do the pretreatments on board for the
> majority of our stains.
> We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are
> cutting and staining between 200-350 slides per day. I think this is a high
> volume for only two people. What do you all think?
>
>
>
> IMPORTANT WARNING: The information in this message (and the documents
> attached to it, if any) is confidential and may be legally privileged. It is
> intended solely for the addressee. Access to this message by anyone else is
> unauthorized. If you are not the intended recipient, any disclosure,
> copying, distribution or any action taken, or omitted to be taken, in
> reliance on it is prohibited and may be unlawful. If you have received this
> message in error, please delete all electronic copies of this message (and
> the documents attached to it, if any), destroy any hard copies you may have
> created and notify me immediately by replying to this email. Thank you.
>
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plau...@cellnetix.com
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RE: [Histonet] Staffing for IHC

[Sebree Linda A]

Your " I think this is a high volume for only two people" is an
understatement!  We have two techs also and cut/stain anywhere from 20 -
150 slides/day on a 7:30 - 4 pm shift and there are times when we're
pulling our hair out.   Your techs must be completely over the edge; get
them some help.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, December 17, 2009 11:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Staffing for IHC


I'm hoping some of the larger hospitals out there will share their data
with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. 
A small portion of our staining protocols still require pretreatment by
hand, but we run instruments that do the pretreatments on board for the
majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd
shift.Together they are cutting and staining between 200-350 slides per
day. I think this is a high volume for only two people. What do you all
think?



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It is intended solely for the addressee. Access to this message by
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taken, in reliance on it is prohibited and may be unlawful. If you have
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hard copies you may have created and notify me immediately by replying
to this email. Thank you.

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[Histonet] Re: FNA stain

[Matthew Lunetta]

Debbie,

We use the Diff-Quick stain for immediate evaluation.

Matt Lunetta HT (ASCP)
Longmont United Hospital
Longmont Colorado


Message: 2
Date: Wed, 16 Dec 2009 13:24:10 -0500
From: dkb...@chs.net
Subject: [Histonet] FNA stain
To: histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset="US-ASCII"

This question is for those of you who perform fine needle aspirations. 
What stain are you using for your immediate evaluation? Or do you give an 
immediate evaluation/adequacy?
Thanks.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net
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[Histonet] veterinary lab question - chem expiration

[Jan Shivers]

What are veterinary labs (non-CAP inspected labs) setting as their expiration 
dates for dry and liquid chemicals that come without expiration dates on the 
labels?

On the Histonet I've found varying answers from human labs (5-10 yrs for dry; 
1-5 yrs for liquid).  I'm in the process of setting guidelines for my labs, but 
want to be sure to be in agreement with other vet labs out there as well.

Thanks,
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
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[Histonet] Staffing for IHC

[Angela Bitting]

I'm hoping some of the larger hospitals out there will share their data with me.
I'm curious as to how labs are staffed for doing IF/IHC/CISH. 
A small portion of our staining protocols still require pretreatment by hand, 
but we run instruments that do the pretreatments on board for the majority of 
our stains.
We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are 
cutting and staining between 200-350 slides per day. I think this is a high 
volume for only two people. What do you all think?



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prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
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Re: [Histonet] New processor help

[Rene J Buesa]

And I think that is what everybody do and I also consider it is more than 
enough. 
Just make sure that the OK is given by the pathologist and that you keep the 
test slides in file and all the paperwork handy for any inspection.
Another thing: do it but do not offer the information to any inspector. Just be 
prepared in case you are asked.
René J.

--- On Thu, 12/17/09, kim.dona...@bhcpns.org  wrote:


From: kim.dona...@bhcpns.org 
Subject: Re: [Histonet] New processor help
To: "Carole Ruffin" 
Cc: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu
Date: Thursday, December 17, 2009, 11:57 AM


Hi Carol, 
                   I am not aware of any procedure that is regulated for 
this. When we have processor problems or purchase a new one we usually run 
test on each specific protocol. ie: bx's, fat run etc. We just make up 
some blocks from scrap specimens and run them through. We log the outcome 
in our QA logs. 

Hope this helps




Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



"Carole Ruffin"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
12/16/2009 12:12 PM

To

cc

Subject
[Histonet] New processor help






Is there a procedure or some standard method validation process out there
for starting up a new tissue processor? Maybe one that would be approved
by regulatory agency? 
I am not sure whether this would just be left up to the pathologists to 
decide what they would prefer.

Thank you,

Carole Ruffin, HT(ASCP)
ValleyCare Hospital
Pleasanton, CA







This message and any included attachments are from ValleyCare Health 
System 
and are intended only for the addressee(s). The information contained 
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may include trade secrets or privileged or otherwise confidential 
information.
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Re: [Histonet] New processor help

[Kim . Donadio]

Hi Carol, 
   I am not aware of any procedure that is regulated for 
this. When we have processor problems or purchase a new one we usually run 
test on each specific protocol. ie: bx's, fat run etc. We just make up 
some blocks from scrap specimens and run them through. We log the outcome 
in our QA logs. 

Hope this helps




Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



"Carole Ruffin"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
12/16/2009 12:12 PM

To

cc

Subject
[Histonet] New processor help






Is there a procedure or some standard method validation process out there
for starting up a new tissue processor? Maybe one that would be approved
by regulatory agency? 
I am not sure whether this would just be left up to the pathologists to 
decide what they would prefer.

Thank you,

Carole Ruffin, HT(ASCP)
ValleyCare Hospital
Pleasanton, CA







This message and any included attachments are from ValleyCare Health 
System 
and are intended only for the addressee(s). The information contained 
herein
may include trade secrets or privileged or otherwise confidential 
information.
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this
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[Histonet] RELIA Histology Job Alert and a last minute shopping tip!

[Pam Barker]

Hi Histonetters!!
I hope everybody is having a great day!  I have a last minute shopping tip
for you... Today is National Free Shipping Day so if you have any online
shopping left to do here is the website:  
www.freeshippingday.com  
Also I wanted to give you a heads up on my current openings.  All of my
clients offer excellent compensation benefits and relocation assistance.
They are willing to interview right away or after the holidays whichever is
more convenient for you.
Here is a list of my current openings:

HISTOLOGY/PATHOLOGY  MANAGEMENT

WI – Waukesha AP Team Leader

NY- Orange/Rockland County Histology Supervisor

KS – Topeka AP Manager

MA – Cape Cod Pathology Supervisor

NC – Charlotte Histology Manager

OR-Portland-Pathology Manager

WA-Spokane-Histology Supervisor-Hospital

CA – Central CA – Pathology Supervisor

CA – Los Angeles –Histology Supervisor

 

HISTOTECHS

LA – Lead IHC Tech –private lab

FL – Pensacola- Histotech private lab

MI – Jackson – Grossing Histotechnologist HTL req,  Private lab

TX – Austin –Night Shift Histotech

TX – Fort Worth  MOHS Tech part time

MA – North Shore of Boston – Histotech 2nd or 3rd shift

NY-Orange/Rockand County-Brand New Lab NYS license req

NY-Upstate NY-NYS license req

NY-NYC-night shift NYS license req

FL- Largo-part time FL lic req

 

Pathology Assistant

NC – Charlotte PA grad from NAACLES program required

 

If you or anyone you know might be interested in any of these opportunities
please contact me.

Thanks-Pam

 

Happy Holidays!!


Thank You!
 
Pam Barker
President
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
Toll Free: (866)607-3542
FAX: (407)678-2788
E-mail:   rel...@earthlink.net <
 mailto:rel...@earthlink.net>
<<  http://home.earthlink.net/~relia1>>
  www.myspace.com/pamatrelia 
  www.twitter.com/pamatrelia

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[Histonet] ThermoScientific stainers

[Joyce Cline]

Does anyone have the Gemini ES stainer and the Clearvue cloverslipper? How is 
the daily performance? Problems?

Thank you
Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941


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[Histonet] Lab chair

[Jones, Lynne]

Hello -
I'm a bit late replying (and can't locate the e-mail address of the OP), but I 
hope the information below is useful.  I have rather strong opinions on this 
subject :)  Our technicians can easily spend 6-8 hours seated at the bench, and 
after 15 years at the bench myself, I understand that proper seating (and 
lighting and instruments) is a necessity, not a luxury.

When we moved into a new facility, I wanted ergonomic chairs that were durable, 
(relatively) solvent resistant and easily cleaned. We work with biological 
material, solvents and radioactivity, and often perform hours of gross 
dissection. I had used several styles of Safco seating in another facility and 
even after 7 years they showed minimal wear.

Similar but less expensive lab stools/chairs are sold by some of the large 
science and office supply companies and with whom we have contracts, so I had 
to plead my case that the Safco chairs I selected were not an extravagance.  
(Office and lab seating was in the process of being standardized as a 
cost-saving measure, and so it was an opportune time for input from folks in 
the lab.)  Several vendors provided demo chairs and I brought over a Safco 
chair and stool as well as one of the less expensive lab chairs to illustrate 
typical wear.  (When the outer skin wears off the less expensive foam chairs, 
from physical wear and exposure to cleaning solvents, they become more porous 
and removal of radioactive contamination is more challenging.) Everybody got to 
try out all the chairs, and we talked about the pros and cons of  specific 
features, and also the challenge of finding chairs to fit employees with 
varying proportions.   We also discussed features that should be avoided for 
lab seating - like mesh backs in any lab that works with blood or biohazards.  
(Some vendors don't seem to understand the difference between office and lab 
seating.)

You may find other models that fit your needs, but in my experience, adjustable 
backs and seats that tilt to adjust significantly ease the inevitable fatigue 
that comes from sitting for >4 hours as do foot rings, while fixed armrests set 
an improper height can increase strain.  I have no affiliation with the Safco 
company, but have been very impresses with the SitStar stool series and the 
Taskmaster 5113 seating.

Here is the website: http://www.safcoproducts.com/
SitStar 6660 stools (all with optional backs).
SitStar 6659 stools (one with no back, two with backs).
Optional SitStar 6661 backs to go with the stools.
Taskmaster 5113 chairs (two with arm-rests).
Optional Taskmaster 5144 T-Bar Armrests to go with the chairs.

My views are my personal opinions and don't represent my employer, but without 
ergonomic seating, I know my own productivity would be reduced.

Lynne Jones

> 
>
> From: histonet-boun...@lists.utsouthwestern.edu on behalf of
> Manalac, Rosa
> Sent: Mon 12/14/2009 12:24 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Lab chair
>
>
>
> Can anyone suggest a comfortable and ergonomic chair to use while
> working with the microtome for many hours each day?  I would
> appreciate
> information on the brand, model, or type and where to order it.
> Even a
> website would help.  Thank you.
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> The information contained in this message may be privileged and/or
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--

Message: 3
Date: Mon, 14 Dec 2009 15:36:22 -0500
From: "Bonner, Janet" 
Subject: RE: [Histonet] Lab chair
To: "Akemi Allison" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
  <5f31f38c96781a4fbe3196ebc22d47807f2...@fhosxchmb006.adventistcorp.net>

Content-Type: text/plain; charset=iso-8859-1

The chairs have to have vinyl seats and back supports, not cloth, so they can 
be easily washed.
  Janet




From: Akemi Allison [mailto:akemiat3...@yahoo.com]
Sent: Mon 12/14/2009 1:36 PM
To: Bonner, Janet
Cc: Manalac, Rosa; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Lab chair


Whatever chair 

RE: [Histonet] hsNFS(INI1) antibody

[Houston, Ronald]

Cell Marque, clone MRQ-27, gives excellent results on the BondMax after
EDTA retrieval.

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree
Linda A
Sent: Thursday, December 17, 2009 10:14 AM
To: Histonet
Subject: [Histonet] hsNFS(INI1) antibody

Hi,

I'm looking for a reference lab that performs an IHC stain for this
antigen.  Its used for diagnosing malignant rhabdoid tumor where this
antigen is lost.  It is expressed in normal tissue.

Thanks,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


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[Histonet] hsNFS(INI1) antibody

[Sebree Linda A]

Hi,

I'm looking for a reference lab that performs an IHC stain for this
antigen.  Its used for diagnosing malignant rhabdoid tumor where this
antigen is lost.  It is expressed in normal tissue.

Thanks,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


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[Histonet] RE: FNA stain

[Lecorchick, William]

Here we provide onsite adequacy for every FNA. The normal is for every pass 
preformed we make two smears one is air dried and stained using Diff-Quick, and 
the other is fixed in 95% ETOH using Pap or H&E.It's a fast and easy to do 
bedside in cases with bilateral nodules as the Radiologist is switching from 
the left to the right side of the bed we can stain the first nodule before he 
has the second started.

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[Histonet] IHC marker RANKL protein

[Bliven, Laura]

I am looking for a reference lab that will stain and interpret the IHC marker 
RANKL.
Any information would be appreciated. Thanks,
Laura
bliven.la...@marshfieldclinic.org

Laura Bliven, AAS, HT(ASCP), QIHC
Histology Lab/IHC
Marshfield Laboratories
1000 N. Oak Ave.
Marshfield, WI 54449



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Re: [Histonet] Silver Nitrate instead of inking??

[Rene J Buesa]

Silver nitrate will react in the skin and transform in silver chloride BUT in 
order to get black it needs to be exposed to solar or very strong light, and 
the reaction is not instantaneous.
Your PT seems that at some point in his/her life s/he got silver nitrate in the 
skin that transformed into a black stain. but that will not readily "fly" in a 
lab setting.
René J.





From: sheila adey 
To: histonet@lists.utsouthwestern.edu
Sent: Thu, December 17, 2009 6:15:17 AM
Subject: [Histonet] Silver Nitrate instead of inking??



Hi Everyone,



Has anyone ever heard of using Silver Nitrate for inking skin bx's before 
processing???

One of our paths mentioned it. 



Thanks in advance. 


Sheila Adey HT MLT 


                        
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[Histonet] Silver Nitrate instead of inking??

[sheila adey]

Hi Everyone,

 

Has anyone ever heard of using Silver Nitrate for inking skin bx's before 
processing???

One of our paths mentioned it. 

 

Thanks in advance. 


Sheila Adey HT MLT 


  
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[Histonet] RE: leaving IHC slides in wash buffer

[Maria Katleba]

The rule is buffer no longer than 1 hour... so it will definitely compromise 
your IHC stain where the antibody may already be marginal.  Sorry
Maria Katleba HT(ASCP) MS
Napa Ca

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, 
Richard E.
Sent: Thursday, December 17, 2009 1:27 AM
To: 'Jennifer Campbell'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: leaving IHC slides in wash buffer

Why?, and why  introduce  such an apparently unnecessary variable?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
Campbell
Sent: 16 December 2009 18:35
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] leaving IHC slides in wash buffer

Hi Everyone,

  I have yet another IHC-related question.  So after antigen retrieval,
and then allowing my slides too cool, I place them in wash buffer before
loading them on to the autostainer.  Would leaving the slides in the
wash buffer in the fridge for an extended period of time (say, 1 week)
before continuing with immunostaining affect staining quality in any
way?

Thank you,

Jen Campbell
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[Histonet] RE: leaving IHC slides in wash buffer

[Edwards, Richard E.]

Why?, and why  introduce  such an apparently unnecessary variable?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
Campbell
Sent: 16 December 2009 18:35
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] leaving IHC slides in wash buffer

Hi Everyone,
 
  I have yet another IHC-related question.  So after antigen retrieval,
and then allowing my slides too cool, I place them in wash buffer before
loading them on to the autostainer.  Would leaving the slides in the
wash buffer in the fridge for an extended period of time (say, 1 week)
before continuing with immunostaining affect staining quality in any
way?
 
Thank you,
 
Jen Campbell
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