Re: [Histonet] GUS staining on parifin thin sections, or infiltration os stain into developing embryos

[John Kiernan]

The following three papers indicate that attention to technical details is 
important in the detection of beta-glucuronidase as a reporter gene. 
- - -
1. Caissard JC, Guivarch A, Rembur J, Azmi A, Chriqui D (1994) Spurious 
localizations of diX-indigo microcrystals generated by the histochemical GUS 
assay. Transgenic Research 3: 176-181.

2. Tague BW, Gallant P, Goodman HM (1997) Expression analysis of an Arabidopsis 
C2H2 zinc finger protein gene. Plant Molecular Biology 32: 785-796.
 
3. Guivarch A, Caissard JC, Azmi A, Elmayan T, Chriqui D, Tepfer M   1996
In situ detection of expression of the gus reporter gene transgenic plants: Ten 
years of blue genes 
Transgenic Research 5 (5): 281-288 SEP 1996 
Abstract: 
Among the methods now available to localize the sites of gene expression in 
plant materials, reporter genes based on the gus (uidA) gene of Escherichia 
coli, which encodes a beta-glucuronidase (E.C. 3.2.1.31; GUS), have been the 
most widely used during the last ten years. The apparent simplicity of the 
histochemical GUS assay has been a major factor in the increase in articles 
using gus genes. However, over the last four years, there have been occasional 
reports expressing doubts concerning the specificity of the observed 
localizations, based on discrepancies between results obtained with GUS 
histochemistry and immunocytochemistry and/or in situ hybridization. This brief 
review compares the results obtained with immunocytochemistry with those 
obtained with various GUS substrates for histochemical studies. Certain sources 
of artefact are discussed, as are the limits that should be imposed on 
interpretation of GUS histochemistry results at the organ, tissue and cell 
levels. 
- - -
 
X-gal, X-gluc etc are nicknames for bromo-chloro-indoxyl glycosides, which can 
generate insoluble indigo-like pigments after enzyme-catalysed hydrolysis 
followed immediately by oxidation. The same X-* substrates are used also in 
methods that trap the products as insoluble azo dyes or as formazans. These 
methods are from the Golden Age of enzyme activity histochemistry (1960s and 
1970s). 
 
A recent review of indigogenic substrates (Indigogenic substrates for the 
detection and localization of enzymes. Biotechnic & Histochemistry 82, 73-103, 
2007) covers many sources of false-positive and false-negative artifacts. 
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: Michelle Jamison 
Date: Thursday, January 7, 2010 18:30
Subject: [Histonet] GUS staining on parifin thin sections, or infiltration os 
stain into developing embryos
To: histonet@lists.utsouthwestern.edu

> Hi All
> I have been trying to stain for GUS expression (X-Gluc) in 
> developing seeds of Arabidopsis.  I have good expression on 
> my positive control (a "housekeeping gene) except for the 
> developing embryos!  And forget about those samples with 
> the specific gene in the embryos.  One of the things I will 
> be trying is thin sectioning then staining.  Possibly the 
> enzyme will be compromised?  
> Does anyone have experience with this sort of thing? 
> Please let me know your thoughts and or experiences.
> Thank you
> Michelle
> 
>  
> 
> Michelle Jamison
> Cytology 
> Caisson labs
> 1740 Research Parkway
> North Logan Utah
> 84341
> 435.755.7617 ext 109
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[Histonet] GUS staining on parifin thin sections, or infiltration os stain into developing embryos

[Michelle Jamison]

Hi All
I have been trying to stain for GUS expression (X-Gluc) in developing seeds of 
Arabidopsis.  I have good expression on my positive control (a "housekeeping 
gene) except for the developing embryos!  And forget about those samples with 
the specific gene in the embryos.  One of the things I will be trying is thin 
sectioning then staining.  Possibly the enzyme will be compromised?  
Does anyone have experience with this sort of thing? 
Please let me know your thoughts and or experiences.
Thank you
Michelle

 

Michelle Jamison
Cytology 
Caisson labs
1740 Research Parkway
North Logan Utah
84341
435.755.7617 ext 109
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RE: [Histonet] pH of 10% NBF

[Tony Henwood]

Greg,

A pH "around" 7 is the aim:

Abler et al (J Histotechnol 6(4):181-184,1983) surveyed several neutral
buffered formalin solutions received from different laboratories and
found a great variation in osmolarity, pH, buffering capacity and
formaldehyde concentration. They recommend the following quality control
parameters be routinely checked on neutral buffered formalin solutions
prepared and used in histopathology laboratories:

Osmolarity  1,000 - 1,600 milliosmols
pH  6.5 - 7.5
Buffering Capacity *Greater than 0.75ml
Formaldehyde Concentration  3 - 5%w/v

* Buffering Capacity is obtained by titrating the amount of 0.1N
hydrochloric acid required to lower the pH of the solution to 2

Comparing the osmolarity in the above table with that recommended for
Millonig's buffered formalin obviously indicates some disagreement. The
importance of osmolarity in fixation is debatable. Carson et al (Am
J.Clin Pathol 59:365, 1973) recommend that Millonig's Phosphate Buffered
Formalin should have a pH of 7.4 and the effective osmolarity is about
290mOsM. 

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Greg
Dobbin
Sent: Friday, 8 January 2010 5:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] pH of 10% NBF


Hi Folks,
If the pH of our 10% Neutral Buffered Formalin is reading 7.01
(recycled) and 7.12 (made from new formaldehyde), should I try to get to
7.2? If yes-how- add sodium bicarbonate? 
I look forward to your responses. 
Warm regards,
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


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[Histonet] California Histotech?

[Breeden, Sara]

I need some assistance and am looking for a contact HT in the San Diego
area.  If that's you and you're willing to answer a couple questions of
a general histology nature, please reply to me directly (not via
Histonet).   This is not a vendor issue and is not a job-search.
Thanks!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

 

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[Histonet] phosphoSMAD-2

[Margaryan, Naira]

Hi histonetters,

I am looking for a good Ab for IHC for phospho-SMAD-2 on mouse tissue.
Any suggestions on company and protocol is appreciated.

Thanks in advance,
Naira 

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[Histonet] Histotech Needed In Seattle

[Erik Dokken]

On Assignment Healthcare in Seattle is currently looking for a qualified 
Histotech to complete a one month contract with one of our clients in Seattle, 
WA.

If you or someone that you know is interested in receiving additional 
information about this position please do not hesitate to contact us.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Thursday, January 07, 2010 10:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 74, Issue 6

Send Histonet mailing list submissions to
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To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. IHC recommendations paper - FYI (Liz Chlipala)
   2. Policy on Floaters (Cho, Soo-Jin)
   3. NYS Licensing Examination Update (NYSHisto)
   4. Re: Policy on Floaters (Rene J Buesa)
   5. Re: Policy on Floaters (Bill)
   6. RE: Policy on Floaters (Blazek, Linda)
   7. Re: Policy on Floaters (Mark Tarango)
   8. special stains (Jennifer Campbell)
   9. RE: Policy on Floaters (Terri  Braud)
  10. Re: Policy on Floaters (Rene J Buesa)
  11. Re: Policy on Floaters (Marcia Funk)
  12. Cytokeratin 17 (Nicholas David Evans)
  13. Re: Policy on Floaters (dkb...@chs.net)
  14. RE: Policy on Floaters (Edwards, Richard E.)
  15. osteopontin antibody (Michele Wich)
  16. getting rid of surplus equipment-Source? (Madary, Joseph)
  17. RE: Policy on Floaters (Merced M Leiker)
  18. RE: Policy on Floaters (Morken, Tim)
  19. CMV positive tissue (Hammel, Vicky)
  20. cmv positive tissue (Hammel, Vicky)


--

Message: 1
Date: Wed, 6 Jan 2010 11:30:22 -0700
From: "Liz Chlipala" 
Subject: [Histonet] IHC recommendations paper - FYI
To: 
Message-ID:

Content-Type: text/plain;   charset="us-ascii"

A lot of people have asked me for the paper that I mentioned in my
e-mail yesterday.  I think I found it on the CAP website, but here is
the papers title and authors.  It's a review article.  Its helped me a
lot with IHC validation.



VM114 Standardization and Troubleshooting

Immunohistochemistry for Pathologists

Neal S. Goldstein, MD

October 2, 2007 8:30 AM - Noon



Recommendations for Improved Standardization

of Immunohistochemistry

Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD,
DPhil,

Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee

On Immunohistochemistry Standardization







Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949

fax (303) 682-9060

www.premierlab.com





Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504





--

Message: 2
Date: Wed, 6 Jan 2010 10:45:53 -0800
From: "Cho, Soo-Jin" 
Subject: [Histonet] Policy on Floaters
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

<622d25c2d399ac40a7cdcf12351b5151fb8ca...@exmbmcb12.ucsfmedicalcenter.org>

Content-Type: text/plain; charset=iso-8859-1

Hello, I'm a resident at UCSF currently working on a QA/QI project regarding 
floaters, with the ultimate goal of formulating a departmental policy regarding 
floaters.  Despite extensive searching on the internet and the Histonet 
archives, I have not found any concrete examples of policies at other 
institutions and was hoping someone could help me out in this regard.  Thank 
you in advance for your help.

Most sincerely,
Soo-Jin Cho
Anatomic Pathology
University of California, San Francisco




--

Message: 3
Date: Wed, 6 Jan 2010 11:33:57 -0800 (PST)
From: NYSHisto 
Subject: [Histonet] NYS Licensing Examination Update
To: Histonet List 
Cc: Amy Farnan 
Message-ID: <969352.91010...@web58803.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Everyone
We have excellent news..!! we?have just received this update from the New 
York State Office of Professions regarding the examination procedures and 
eligibility for licensing in NYS, Please see below.?
Since this is "hot off the press" information, I am sure there will be 
questions but please be patient as the procedures are formally written and 
posted on the website (http://www.op.nysed.gov/).??For those of you who want to 
be updated regularly on whats going on in NY, please join the New York State 
Histotechnological Society message board 
at:?http://tech.groups.yahoo.com/group/NYSHS1972/?If you have questio

[Histonet] pH of 10% NBF

[Greg Dobbin]

Hi Folks,
If the pH of our 10% Neutral Buffered Formalin is reading 7.01
(recycled) and 7.12 (made from new formaldehyde), should I try to get to
7.2? If yes-how- add sodium bicarbonate? 
I look forward to your responses. 
Warm regards,
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


-
Statement of Confidentiality
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received this communication in error, please notify the sender immediately. If 
you are not the intended recipient, you are not authorized to use, disclose, 
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email from your entire computer system.




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[Histonet] Re: Policy on Floaters

[Robert Richmond]

Dr. Soo-Jin Cho asks:

>>Hello, I'm a resident at UCSF currently working on a QA/QI project regarding 
>>floaters, with the ultimate goal of formulating a departmental policy 
>>regarding floaters.  Despite extensive searching on the internet and the 
>>Histonet archives, I have not found any concrete examples of policies at 
>>other institutions and was hoping someone could help me out in this regard.<<

Several people have already offered some very useful advice. From the
pathologist's viewpoint, I'd distinguish between section floaters
(which usually aren't the direct concern of the pathologist or
pathologist's assistant) and tissue floaters (which definitely are).
The usual cause of tissue floaters is carry-over of tissue fragments
during grossing. I almost never have this problem myself - where I've
seen problems is with an overloaded P.A. who's working too fast.

I've always been ridiculed for proposing that it would be worthwhile
to have about five sets of the basic dissecting tools (scalpel,
scissors, ruler, tweezers, etc.) and toss them into a pot of water
between each case, then stop and wash them off after five cases. (This
arrangement would of course violate the Good Management Principle that
a pathologist may have only one set of tools.)

Certain cases - such as papillary urinary tract and ovarian tumors -
are notorious creators of floaters and should be grossed last, or
right before the placentas at any rate.

The worst floater incident I've ever seen occurred in a small lab that
mostly saw skin biopsies. A lens paper wrapper (this was over 20 years
ago) came open in processing and spilled out the cell block contents
from a peritoneal fluid specimen. The specimen contained papillary
fragments from an ovarian tumor. I found those fragments in the slides
from 26 different cases (fortunately none of them was diagnostically
difficult). Follow-up with the attending physician found that he was
totally surprised by the results - the cancer was quite unsuspected in
a woman with rapidly progressing ascites.

I was taught to write "floater" on the slide with an appropriate pen,
and initial the slide also. The problem should almost never be
mentioned in the pathologist's report.

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] Policy on Floaters

[Bill B.]

At 9:19 AM -0800 1/7/10, Morken, Tim wrote:
>I assure you "floaters" are not trivial in a diagnostic setting. Imagine a 
>case biopsied for suspected melanoma. The tissue appears clear of melanoma but 
>on the edge there is a small piece that is suspicious. But other slides do not 
>show that piece and it is not in the block. Was it cut through? Is it a 
>floater? Is it real? Investigations ensue. Even genetic testing of the 
>fragment may be done to resolve the issue. <<<

Yes, but most floaters are obvious. Most common, IME, are chorionic villi. 
Granted a rare one can be a diagnostic conundrum and must be worked up 
appropriately. I think it a waste of time and resources to investigate and 
document every one encountered, beyond marking them on the slide as a floater. 
If they are becoming common then their source must be investigated. 

Bill


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Re: [Histonet] cmv positive tissue

[Mark Tarango]

Sorry everyone, I didn't mean to send that to the whole histonet.  Please
respond privately Vicky.

On Thu, Jan 7, 2010 at 10:12 AM, Mark Tarango  wrote:

> I can trade you.  I have a few prepared control blocks that we use for
> IHC.  I'd be willing to trade for malignant melanoma.  In Seattle, we don't
> get enough sun to see many melanoma cases.  If you know that its postive for
> hmb-45, mart-1/melan-a, and S100 it'd be my dream come true.
>
> Let me know!
>
> Thanks
>
> Mark Tarango HT(ASCP)QIHC
> Cellnetix Pathology
> 1124 Columbia Street, Suite 200
> Seattle, Wa  98104
>   On Thu, Jan 7, 2010 at 9:50 AM, Hammel, Vicky wrote:
>
>> What are labs using for positive tissue for CMV IHC? If anyone is
>> interested
>> in trading tissue (slides or blocks) please contact me.
>>
>> Thank you,
>>
>>
>>
>> Vicky Hammel HTL, ASCP
>>
>> Pathology Technical Consultant
>>
>>
>>
>> vham...@primecare.org 
>>
>>
>>
>> St. Alexius Medical Center
>>
>> Histology Laboratory
>>
>> 900 east Broadway
>>
>> Bismarck, ND 58506
>>
>>
>>
>>
>>
>> ___
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>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
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Re: [Histonet] cmv positive tissue

[Mark Tarango]

I can trade you.  I have a few prepared control blocks that we use for IHC.
I'd be willing to trade for malignant melanoma.  In Seattle, we don't get
enough sun to see many melanoma cases.  If you know that its postive for
hmb-45, mart-1/melan-a, and S100 it'd be my dream come true.

Let me know!

Thanks

Mark Tarango HT(ASCP)QIHC
Cellnetix Pathology
1124 Columbia Street, Suite 200
Seattle, Wa  98104
On Thu, Jan 7, 2010 at 9:50 AM, Hammel, Vicky  wrote:

> What are labs using for positive tissue for CMV IHC? If anyone is
> interested
> in trading tissue (slides or blocks) please contact me.
>
> Thank you,
>
>
>
> Vicky Hammel HTL, ASCP
>
> Pathology Technical Consultant
>
>
>
> vham...@primecare.org 
>
>
>
> St. Alexius Medical Center
>
> Histology Laboratory
>
> 900 east Broadway
>
> Bismarck, ND 58506
>
>
>
>
>
> ___
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>
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[Histonet] cmv positive tissue

[Hammel, Vicky]

What are labs using for positive tissue for CMV IHC? If anyone is interested
in trading tissue (slides or blocks) please contact me. 

Thank you,

 

Vicky Hammel HTL, ASCP

Pathology Technical Consultant

 

vham...@primecare.org  

 

St. Alexius Medical Center

Histology Laboratory

900 east Broadway

Bismarck, ND 58506

 

 

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[Histonet] CMV positive tissue

[Hammel, Vicky]

What are labs using for positive tissue for CMV IHC? If anyone is interested
in trading tissue please contact me.

Thank you, Vicky Hammel

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RE: [Histonet] Policy on Floaters

[Morken, Tim]

I assure you "floaters" are not trivial in a diagnostic setting. Imagine a case 
biopsied for suspected melanoma. The tissue appears clear of melanoma but on 
the edge there is a small piece that is suspicious. But other slides do not 
show that piece and it is not in the block. Was it cut through? Is it a 
floater? Is it real? Investigations ensue. Even genetic testing of the fragment 
may be done to resolve the issue. 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker
Sent: Thursday, January 07, 2010 9:02 AM
To: Edwards, Richard E.; 'dkb...@chs.net'; Marcia Funk
Cc: histonet@lists.utsouthwestern.edu; Cho, Soo-Jin
Subject: RE: [Histonet] Policy on Floaters

I second that question! Seems like a lot of hassle (documenting, holding 
meetings) over a bit of waxy tissue left in a water bath (mind you, this is 
coming from a research mind-set where we don't mind these things so much). 
:-)


--On Thursday, January 07, 2010 1:34 PM + "Edwards, Richard E." 
 wrote:

> So, what  exactly is   a  "floater", an extra bit of a previous section
> picked up with the next section in a dirty waterbath, or  an obviously
> extra identifiable piece of tissue  included in/picked up in error during
> grossing;  bit  of  ambiguity  in previous  emails?.
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> dkb...@chs.net Sent: 07 January 2010 13:18
> To: Marcia Funk
> Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho;
> histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy
> on Floaters
>
> Lots of traffic on this one.  Yes you definitely need a policy.  The
> policy should define how you will "try" to eliminate floaters ie;  clean
> water bath after each block, spray/wipe/change paper towels after each
> specimen while grossing etc.  It also must include how it will be
> resolved.  This covers everyone involved and is considered best practice.
>
> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
> Center I
> 200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l
> F:  804-765-5582 l dkb...@chs.net
>
>
>
>
>
>
>
> "Marcia Funk" 
> Sent by: histonet-boun...@lists.utsouthwestern.edu
> 01/06/2010 05:23 PM
>
> To
> "Mark Tarango" , "Soo-Jin Cho"
> 
> cc
> "histonet@lists.utsouthwestern.edu" 
> Subject
> Re: [Histonet] Policy on Floaters
>
>
>
>
>
>
> Floaters
> Yes, you are so right, for patient safety and your safety,  policy is a
> must.  Protects you and the patient.
> Marcia
>
>
> Marcia Funk
> Histology Laboratory
> Mercy Medical Center North Iowa
> Mason City, IA, 50401
> 641-422-7907
>
>
 Mark Tarango  01/06/2010 2:21 PM >>>
> Your goal is not to have floaters.  If you get one, your policy should set
> out to determine the cause of these incidents.  You should track who did
> it
> (in a spreadsheet), where it happened (grossing, embedding, cutting...).
> Then you should have a meeting every so often with people from the lab and
> some pathologists where you go over all the incidents, brainstorm for
> corrective actions, and decide about what you can do differently.
>
> Make sure you have the techs invovled or it won't be very effective.
>
> Mark
>
>
>
>
> On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin
> wrote:
>
>> Hello, I'm a resident at UCSF currently working on a QA/QI project
>> regarding floaters, with the ultimate goal of formulating a departmental
>> policy regarding floaters.  Despite extensive searching on the internet
> and
>> the Histonet archives, I have not found any concrete examples of
> policies at
>> other institutions and was hoping someone could help me out in this
> regard.
>>  Thank you in advance for your help.
>>
>> Most sincerely,
>> Soo-Jin Cho
>> Anatomic Pathology
>> University of California, San Francisco
>>
>>
>> ___
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>>
> ___
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>
>
>
> --
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> is intended only for the use of the individual(s) and entity named
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> message, please notify the sender immediately and delete the
> material from your com

RE: [Histonet] Policy on Floaters

[Merced M Leiker]

I second that question! Seems like a lot of hassle (documenting, holding 
meetings) over a bit of waxy tissue left in a water bath (mind you, this is 
coming from a research mind-set where we don't mind these things so much). 
:-)



--On Thursday, January 07, 2010 1:34 PM + "Edwards, Richard E." 
 wrote:



So, what  exactly is   a  "floater", an extra bit of a previous section
picked up with the next section in a dirty waterbath, or  an obviously
extra identifiable piece of tissue  included in/picked up in error during
grossing;  bit  of  ambiguity  in previous  emails?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
dkb...@chs.net Sent: 07 January 2010 13:18
To: Marcia Funk
Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho;
histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Policy
on Floaters

Lots of traffic on this one.  Yes you definitely need a policy.  The
policy should define how you will "try" to eliminate floaters ie;  clean
water bath after each block, spray/wipe/change paper towels after each
specimen while grossing etc.  It also must include how it will be
resolved.  This covers everyone involved and is considered best practice.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l
F:  804-765-5582 l dkb...@chs.net







"Marcia Funk" 
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/06/2010 05:23 PM

To
"Mark Tarango" , "Soo-Jin Cho"

cc
"histonet@lists.utsouthwestern.edu" 
Subject
Re: [Histonet] Policy on Floaters






Floaters
Yes, you are so right, for patient safety and your safety,  policy is a
must.  Protects you and the patient.
Marcia


Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907



Mark Tarango  01/06/2010 2:21 PM >>>

Your goal is not to have floaters.  If you get one, your policy should set
out to determine the cause of these incidents.  You should track who did
it
(in a spreadsheet), where it happened (grossing, embedding, cutting...).
Then you should have a meeting every so often with people from the lab and
some pathologists where you go over all the incidents, brainstorm for
corrective actions, and decide about what you can do differently.

Make sure you have the techs invovled or it won't be very effective.

Mark




On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin
wrote:


Hello, I'm a resident at UCSF currently working on a QA/QI project
regarding floaters, with the ultimate goal of formulating a departmental
policy regarding floaters.  Despite extensive searching on the internet

and

the Histonet archives, I have not found any concrete examples of

policies at

other institutions and was hoping someone could help me out in this

regard.

 Thank you in advance for your help.

Most sincerely,
Soo-Jin Cho
Anatomic Pathology
University of California, San Francisco


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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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However, many electrons were severely inconvenienced.


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[Histonet] getting rid of surplus equipment-Source?

[Madary, Joseph]

Does anyone have a good source to get rid of equipment that is still
good and maybe our lab can make a few bucks in the process?  The last
time I did this I was told the processor and stainer I gave away would
be going to some charity and of course it did not I found out later.
These items are off the books, maybe in need of a small repair.  We have
no room and the equipment is just been depreciated such that if we can
get a credit or something for our lab as a trade in.  Specifically we
have an RMS microtome in perfect condition used for 6 months and put
away.  The other is a Leica Embedder that does everyting but dispense
paraffin.  We were using a separate paraffin dispenser and it was fine,
but finally got another embedder.  Anyway, vendors welcome.

 

Nick Madary, HT/HTL(ASCP)QIHC

Medimmune Histology Mgr, 

OMW, Area 4, Lab 2438

301.398.4745(vm)

301.398.6360(lab)

301.398.9745(fax)

 




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[Histonet] osteopontin antibody

[Michele Wich]

Can anyone recommend an osteopontin antibody that works in FFPE mouse
tissue?




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RE: [Histonet] Policy on Floaters

[Edwards, Richard E.]

So, what  exactly is   a  "floater", an extra bit of a previous section picked 
up with the next section in a dirty waterbath, or  an obviously  extra 
identifiable piece of tissue  included in/picked up in error during grossing;  
bit  of  ambiguity  in previous  emails?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dkb...@chs.net
Sent: 07 January 2010 13:18
To: Marcia Funk
Cc: histonet@lists.utsouthwestern.edu; Soo-Jin Cho; 
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Policy on Floaters

Lots of traffic on this one.  Yes you definitely need a policy.  The 
policy should define how you will "try" to eliminate floaters ie;  clean 
water bath after each block, spray/wipe/change paper towels after each 
specimen while grossing etc.  It also must include how it will be 
resolved.  This covers everyone involved and is considered best practice.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







"Marcia Funk"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/06/2010 05:23 PM

To
"Mark Tarango" , "Soo-Jin Cho" 

cc
"histonet@lists.utsouthwestern.edu" 
Subject
Re: [Histonet] Policy on Floaters






Floaters
Yes, you are so right, for patient safety and your safety,  policy is a 
must.  Protects you and the patient.
Marcia
 
 
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907


>>> Mark Tarango  01/06/2010 2:21 PM >>>
Your goal is not to have floaters.  If you get one, your policy should set
out to determine the cause of these incidents.  You should track who did 
it
(in a spreadsheet), where it happened (grossing, embedding, cutting...).
Then you should have a meeting every so often with people from the lab and
some pathologists where you go over all the incidents, brainstorm for
corrective actions, and decide about what you can do differently.

Make sure you have the techs invovled or it won't be very effective.

Mark




On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin 
wrote:

> Hello, I'm a resident at UCSF currently working on a QA/QI project
> regarding floaters, with the ultimate goal of formulating a departmental
> policy regarding floaters.  Despite extensive searching on the internet 
and
> the Histonet archives, I have not found any concrete examples of 
policies at
> other institutions and was hoping someone could help me out in this 
regard.
>  Thank you in advance for your help.
>
> Most sincerely,
> Soo-Jin Cho
> Anatomic Pathology
> University of California, San Francisco
>
>
> ___
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> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
>
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Re: [Histonet] Policy on Floaters

[DKBoyd]

Lots of traffic on this one.  Yes you definitely need a policy.  The 
policy should define how you will "try" to eliminate floaters ie;  clean 
water bath after each block, spray/wipe/change paper towels after each 
specimen while grossing etc.  It also must include how it will be 
resolved.  This covers everyone involved and is considered best practice.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







"Marcia Funk"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/06/2010 05:23 PM

To
"Mark Tarango" , "Soo-Jin Cho" 

cc
"histonet@lists.utsouthwestern.edu" 
Subject
Re: [Histonet] Policy on Floaters






Floaters
Yes, you are so right, for patient safety and your safety,  policy is a 
must.  Protects you and the patient.
Marcia
 
 
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907


>>> Mark Tarango  01/06/2010 2:21 PM >>>
Your goal is not to have floaters.  If you get one, your policy should set
out to determine the cause of these incidents.  You should track who did 
it
(in a spreadsheet), where it happened (grossing, embedding, cutting...).
Then you should have a meeting every so often with people from the lab and
some pathologists where you go over all the incidents, brainstorm for
corrective actions, and decide about what you can do differently.

Make sure you have the techs invovled or it won't be very effective.

Mark




On Wed, Jan 6, 2010 at 10:45 AM, Cho, Soo-Jin 
wrote:

> Hello, I'm a resident at UCSF currently working on a QA/QI project
> regarding floaters, with the ultimate goal of formulating a departmental
> policy regarding floaters.  Despite extensive searching on the internet 
and
> the Histonet archives, I have not found any concrete examples of 
policies at
> other institutions and was hoping someone could help me out in this 
regard.
>  Thank you in advance for your help.
>
> Most sincerely,
> Soo-Jin Cho
> Anatomic Pathology
> University of California, San Francisco
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
>
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