Re: [Histonet] Slide marking pens

[V. Neubert]

Keep the cap on the pen and always store the pen on its tip (even in the
lab coats pocket). Won't dry out, for sure.

> I have used Secureline Markers as one person suggested, but they seemed to dry
> up fast.  
>
> Peggy
>   

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[Histonet] IHC edge effect

[Jeffrey Silverman]

 





Hi Katelin, 
 
It would be helpful to view some images- can you post them on Histonet? 
Just to be sure: You say you have intense peripheral non-specific staining and 
do not see the expected immunoreactivity in the center of the tissues, or do 
you see expected reactivity centrally but of weaker intensity? 
Don't know what more to tell you but some questions come to mind...
..
Formalin fixed tissues?  Any microwave  processing or fixation going on? 
Antigen retrieval? SMA, in my experience, does not require antigen retrieval. 
 
Are you staining manually or on an instrument?  If instrument, which one? Is it 
calibrated and PM'ed to deliver the proper amount of reagent? The progressive 
involvement and the problem's new appearance in other antibodies suggests maybe 
a deteriorating problem with an instrument's function. 
 
 If manual, do you drag reagent well past the edges of the tssue to more than 
cover each section?  Is only one person doing the stains? ie could it be a 
problem with someone's technique? 
 
How do you dry your sections? Immediately after cutting place in a 60 degree C 
oven. Be sure it is  no more than that, hotter than 60 deg C will damage 
sections and immunoreactivity. Or do you  air dry first and then oven?  This 
might allow lifting of the periphery with subsequent entrapment of reagent 
under the edges that might not be removed with interstep washing. 
 Just some more ideas. 
 
Jeff Silverman HT HTL QIHC (ASCP)
Pathologists' Assistant
Southside Hospital - NSLIJ Health System

--- On Wed, 1/13/10, Katelin Lester  wrote:


From: Katelin Lester 
Subject: Re: [Histonet] Fw: Waste paraffin and edge effect
To: "Jeffrey Silverman" 
Date: Wednesday, January 13, 2010, 12:33 PM


Hi Jeffrey,
I'm curious about what you said about the edges of the tissue having more
fixation than the rest of the tissue. I'm curious because that would make
sense with some of the uterus sections we are using for our control for
SMA, but I'm not sure how that would work for the patient tissue as well.
Every SMA I run, control and patient, any area of the slide shows this
dark edge staining with minimal staining in the center, as well as
nonspecific. We are also seeing it in CK AE1/AE3 and our CD117 has started
demonstrating it on previously perfect controls.  Any other ideas or
suggestions?
Katelin Lester, HTL(ASCP)
MedSurg Pathology Associates, Inc.
(503)443-2157
>
>
> ---
>
>
>
>
>
>
>
> Hello everyone,
>  
> Our system's  hazardous chemical waste consultants changed our method of
> disposal- we had been solidifying the paraffin in a hood and then red
> bagging it, ie treating it as regulated medical (biohazard) waste.
>  
> But, due to it's contamination with xylene, the very reason for our
> disposing of it, the paraffin is really hazardous chemical waste. Believe
> it or not, EPA regs prohibit most hospitals from treating their chemical
> waste and the consultants worried that solidifying it under the hood was
> considered "treating" the waste.  Yeah right!!   Anyway, the blocks of
> solidified paraffin are now dumped in it's own specially labelled drum and
> has become a part of our hazardous waste stream, manifested like the
> xylene and dye waste. I'd be careful about putting hazardous chemicals
> into regulated medical waste.
>  
> Interestingly, our system recently inquired if formalin in discarded
> surgicals needs to be decanted before disposal- many hospitals are doing
> just that, but most of those, but not all, recycle formalin (yuk!). Our
> disposal facility informed us that as long as the containers are plastic,
> ie are flammamble, they can be incinerated with the formalin and there is
> no need to decant.  Glory be!!
>  
> In IHC slides, edge effect, or more intense, sometimes nonspecific,
> staining at the periphery of the tissue, can be caused  by more intense
> fixation of a block at the periphery, drying out of the edges of a block
> before fiation,  and/or by some degree of drying of antibodies and
> detection chemistry reagents during staining- this cause is more common in
> manually stained slides rather than those stained on automated stainers.  
>  
> Also electrocautery used during the excision can also cause intense
> staining at the edges of specimens.
>  
> Jeffrey S. Silverman HT HTL QIHC (ASCP)
> Southside Hospital- NSLIJ Health System
> Pathologists' Assistant and Laboratory Safety Officer
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[Histonet] RE: Histonet Digest, Vol 74, Issue 12

[Moody, Robert]

Hi, All we are having problems with chatter in our biopsies their usually on 
the edge of tissue is the a problem with the cutting or in the handling of the 
tissue after it is removed from the patient like the biopsies being left out to 
dry or not put in formalin what are some of your experience with this.
Robert Moody HT ASCP
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, January 13, 2010 10:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 74, Issue 12

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Today's Topics:

   1. IHC (Marian Powers)
   2. RE: Eliminating the edge effect in IHC/IF (C.M. van der Loos)
   3. RE: RE: Eliminating the edge effect in IHC/IF
  (Sherwood, Margaret )
   4. RE: Eliminating the edge effect in IHC/IF (Rene J Buesa)
   5. RE: RE: Eliminating the edge effect in IHC/IF
  (Della Speranza, Vinnie)
   6. RE: RE: Eliminating the edge effect in IHC/IF (Rene J Buesa)
   7. RE: RE: Eliminating the edge effect in IHC/IF (Hermina Borgerink)
   8. Fw: Waste paraffin and edge effect (Jeffrey Silverman)
   9. Re: Sakura iDent (Anne van Binsbergen)
  10. Looking for Florida licensed Histologist (Kaye Ryan)
  11. RE: Re: Sakura iDent (Cynthia Pyse)
  12. AW: [Histonet] Eliminating the edge effect in IHC/IF (Gudrun Lang)
  13. Bladder Biopsies (S R)
  14. Re:  Bladder Biopsies (Lester Raff MD)
  15. Re: Eliminating the edge effect in IHC/IF
  (histol...@medsurgpath.com)
  16. Part-time Flex Histologist position (Fallak, Alice)
  17. RE: Re: Sakura iDent (Nancy Klemme)
  18. Re: RE: [Histonet] Problems with MSB stain (John Kiernan)
  19. (Used microtome) - Thanks ... (Massimo)
  20. (Used microtome) - Thanks ... (Massimo)
  21. AW: [Histonet] Problems with MSB stain (Gudrun Lang)
  22. Re: Re: Sakura iDent (Anne van Binsbergen)


--

Message: 1
Date: Tue, 12 Jan 2010 14:34:33 -0500
From: Marian Powers 
Subject: [Histonet] IHC
To: histonet@lists.utsouthwestern.edu
Message-ID:
<5d7de0e61001121134g662d43adr3a7c4cd1b0235...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi:  We are looking to start N-ras and B-raf for IHC, is anyone running
these antibodies on paraffin?  If so, what clone and vendor are you using?
Thanks in advance!



--
Marian L. Powers, HT(ASCP)
Manager, Technical Operations

Doctors Pathology Services
1253 College Park Drive
Dover, DE 19904
302-677-


--

Message: 2
Date: Tue, 12 Jan 2010 20:54:35 +0100
From: "C.M. van der Loos" 
Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu
Cc: k...@prosci-inc.com
Message-ID: <9637c5fe5d7dffc7.4b4ce...@amc.uva.nl>
Content-Type: text/plain; charset=utf-8

Hi all,We also have observed this phenomenon many times. But sorry Colleen and 
Rene, I don't believe that an fixation issue is the explanation why the edges 
are sometimes stronger than the rest. To my opinion this is a bit too easy. One 
of my explanations is that the immuno reagents tend to stick to the edges of 
the tissue section, especially when no Tween20 is involved. As a result the 
outer edges might become a little dry during incubation times and cause darker 
mostly non-specific staining. Always try to cover a section completely, 
including a rim around the section. However, to be honest, I am sure my 
explanation is certainly not always appropriate. Anyone 
elseCheers,ChrisChris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
phone:  +31 20 5665631
 From: Rene J Buesa 
Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu, Karen Cai 

Usually that is the result of incomplete fixation. Check your fixation protocol.
Ren??? J.

--- On Mon, 1/11/10, Karen Cai  wrote:


From: Karen Cai 
Subject: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 2:00 PM


Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?

Your kind help is 

Re: Re: [Histonet] Eliminating the edge effect in IHC/IF

[TF]

Hi all
i believe it is due to the wash + antibody accumulation problem happened 
between the edge of section and the adhesive slide.

Even you have circled the section with IHC pen(DAKO), and flattened all 
soultions evenly, it improved but did not disappear

if you stain the section using "floating section" method, no edge effect now.


2010-01-14 



TF 



发件人： Rene J Buesa 
发送时间： 2010-01-12  13:30:49 
收件人： histonet; Karen Cai 
抄送： 
主题： Re: [Histonet] Eliminating the edge effect in IHC/IF 
 
Usually that is the result of incomplete fixation. Check your fixation protocol.
René J.
--- On Mon, 1/11/10, Karen Cai  wrote:
From: Karen Cai 
Subject: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 2:00 PM
Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?
Your kind help is greatly appreciated,
Thanks in advance,
Best,
Karen
Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
 www.prosci-inc.com
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[Histonet] HTL certification

[Jeffrey Silverman]

>From the ASCP website (just google ASCP and HTL certification)
 
Histotechnologist, HTL(ASCP)
Application Fee: $210
To be eligible for this examination category, an applicant must satisfy the 
requirements of at least one of the following routes:
Route 1: Baccalaureate degree from a regionally accredited college/university 
with a combination of 30 semester hours (45 quarter hours) of biology and 
chemistry AND successful completion of a NAACLS accredited Histotechnician or 
Histotechnology program within the last 5 years; or
Route 2: Baccalaureate degree from a regionally accredited college/university 
with a combination of 30 semester hours (45 quarter hours) of biology and 
chemistry AND one year full time acceptable experience in a histopathology 
laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited 
laboratory within the last ten years. This year of experience must be under the 
supervision of a pathologist (certified by the American Board of Pathology in 
Anatomic Pathology) or an appropriately board certified medical scientist.
Clinical Laboratory Experience
To fulfill the experience requirement for the Histotechnologist examination, 
you must have experience, within the last ten years, in the following areas:

Fixation 
Microtomy 
Processing 
Staining 
Jeff Silverman
 
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[Histonet] TMA Repair

[Gross, Jenny]

 

My organization has spent a great deal of time and effort constructing a
tissue microarray.  However, we have just been notified that two out of
the three TMA blocks have cracks in the bottom portion and are coming
off of the cassettes.  I wanted to know what other organizations have
done to repair this issue so the blocks can still be used.

 

Please advise.

 

Jenny Gross, MPH

Manager

Women's Cancer Research Institute

Samuel Oschin Comprehensive Cancer Institute

 

 

Jenny Gross, MPH

Manager

Women's Cancer Research Institute

Samuel Oschin Comprehensive Cancer Institute

Cedars-Sinai Health System

8700 Beverly Boulevard, Suite 290W

Los Angeles, CA 90048

Office:  (310) 423-6241

Cell:  (323) 428-2338

 

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[Histonet] Estrogen Receptor and Progesterone Receptors

[Mary Benoit]

Sorry, I forgot to sign my name.
Mary F Benoit MT(ASCP)
The Pathology Laboratory
830 Bayou Pines Drive
Lake Charles, La
 



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[Histonet] Estrogen and Progesterone Receptors

[Mary Benoit]

We currently are using Dako antibodies for Estrogen and Progesterone Receptors 
then followed with ACIS imaging on all infiltrating breast carcinomas.  We 
generally put a positive control on the bottom of the same slide for each to 
save a little time and money.  Results are great . Fixation is at least 8 hours 
(never more than 18 hours) in NBF on a designated processor. My question is our 
pathologists are concerned when the Estrogen Receptor is strongly positive and 
the Progesterone is very weak or negative.  He states that the results should 
be approximately within 10% of each other.  Our policy is to repeat the IHC if 
there are no internal "normal" ducts within the sample(note that the normal 
ducts, when present, do stain well).  Repeated results are the same.  I've made 
a comparison of diagnosis for tumor types (well diff, mod diff, poorly diff, 
lobular, etc.) and there is an even distribution of these cases.  Does anybody 
have input to this
 situation?  



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[Histonet] Kaiser Permanente

[Angela Bitting]

If someone from Kaiser Permanente is watching the Histonet, would you contact 
me offline about a piece of Histology equipment. My rep. (and I'm not 
mentioning the company!!) is telling me you have something that I'm interested 
in buying, and I'd like your feedback.
 
Thanks,
Angie
 
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
 


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RE: [Histonet] requirements for certification

[Katelin Lester]

Yes, you are correct. One year OJT plus a BA/BS with required # of
biology/chemistry credits for HTL.  When I tried to call to see if I
qualified they said that they can not tell you over the phone. You have to
apply (and pay the $200) to see if you qualify.
Katelin Lester, HTL(ASCP)
MedSurg Pathology Associates, Inc.
(503)443-2157
> You might be eligible to sit for the registry already either HT or HTL,
> you just need to have the correct number of credits in biology and
> chemistry to meet the requirements.  I would call ASCP to see if you
> have the requirements, it seems to me that you have the experience
> needed you just need to make sure you have the credits.  That's my
> opinion.  All you need is one year on the job training with a BS or BA
> to sit for the HTL.  I think I'm correct on this, but if I'm not we'll
> know soon enough from the replys.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949
> fax (303) 682-9060
> www.premierlab.com
>
>
> Ship to Address:
> 1567 Skyway Drive, Unit E
> Longmont, Colorado 80504
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
> Anderson
> Sent: Wednesday, January 13, 2010 2:51 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] requirements for certification
>
> Hello.
>
> I'm thinking about getting my HTL certification.  I've been a bench
> scientist in both academia and biotech for 21 years, which has included
> pre-clinical animal research and subsequent histological applications.
> I've set up an histology lab and have a lot of IHC but only basic
> staining (mostly H&E), as well as loads of animal tissue processing and
> some human tissues.  I have a liberal arts degree (an AB) with a biology
> major and a chemistry minor.  We have both a DVM and a few MD's on site,
> but for pre-clinical and clinical research consult.  I would appreciate
> any insight on getting certified through an online program, how many
> hours is required, and what kind of mentorship is necessary.
>
> Thank you for your insights and time!
>
> Jennifer M. Anderson, Scientist
> Halozyme Therapeutics, Inc.
> 11388 Sorrento Valley Road
> San Diego, CA 92121
> 858-704-8333
> jander...@halozyme.com
>
> 
> The information transmitted in this email is confidential and is
> intended only for the person(s) or entity to which it is addressed.
> Delivery of this message to any person other than the intended
> recipient(s) is not intended in any way to waive confidentiality or any
> applicable privilege. Any review, retransmission, dissemination or other
> use of, or taking of any action in reliance upon, this information by
> individuals or entities other than the intended recipient is prohibited
> by Halozyme and may be in violation of applicable laws. If you received
> this in error, please contact the sender and delete/destroy this email.
> ___
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RE: [Histonet] requirements for certification

[Whitaker, Bonnie]

I agree with Liz.  It seems with a bio major and chem minor, that should take
care of the coursework.  You could do the IU online program, but if you are
self-motivated, I would just study on my own, if I were you.  If you don't
pass the first time, then at least you would know how close you were, and
could decide to take the course, or just to study a little more.  If you can
pass the exam, why waste the time and money unnecessarily?  It sounds like
you probably have a great background and experience already!

Bonnie Whitaker

Clinical Histology Manager
Ohio State University Medical Center
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

bonnie.whita...@osumc.edu

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, January 13, 2010 4:57 PM
To: Jennifer Anderson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] requirements for certification

You might be eligible to sit for the registry already either HT or HTL, you
just need to have the correct number of credits in biology and chemistry to
meet the requirements.  I would call ASCP to see if you have the
requirements, it seems to me that you have the experience needed you just
need to make sure you have the credits.  That's my opinion.  All you need is
one year on the job training with a BS or BA to sit for the HTL.  I think I'm
correct on this, but if I'm not we'll know soon enough from the replys.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO
Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Wednesday, January 13, 2010 2:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] requirements for certification

Hello.

I'm thinking about getting my HTL certification.  I've been a bench scientist
in both academia and biotech for 21 years, which has included pre-clinical
animal research and subsequent histological applications.
I've set up an histology lab and have a lot of IHC but only basic staining
(mostly H&E), as well as loads of animal tissue processing and some human
tissues.  I have a liberal arts degree (an AB) with a biology major and a
chemistry minor.  We have both a DVM and a few MD's on site, but for
pre-clinical and clinical research consult.  I would appreciate any insight
on getting certified through an online program, how many hours is required,
and what kind of mentorship is necessary.

Thank you for your insights and time!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
jander...@halozyme.com


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or entities other than the intended recipient is prohibited by Halozyme and
may be in violation of applicable laws. If you received this in error, please
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RE: [Histonet] Paraffin Disposal

[Sherwood, Margaret]

To all:

I stand corrected!  I asked our hazardous waste tech about the paraffin waste
and he said they should take it (since it has xylene, or in our case, citrisolv
in it).  Live and learn.

Peggy 

-Original Message-
From: Sherwood, Margaret 
Sent: Monday, January 11, 2010 2:05 PM
To: 'Scott, Allison D'
Subject: RE: [Histonet] Paraffin Disposal

Once it is hardened, I put it in the regular waste (haven't had any questions
raised about that practice).  Would be interested in other responses.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Monday, January 11, 2010 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin Disposal

Happy New Year to all.  How are you all disposing the paraffin that
comes off of the tissue processor?  Do you consider it as biohazard
waste and disposing it as such or are you putting it into the regular
trash disposal?  I have been asked why I am disposing it the way I do.
I consider it as biohazard waste.  Your help in this will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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RE: [Histonet] requirements for certification

[Liz Chlipala]

You might be eligible to sit for the registry already either HT or HTL,
you just need to have the correct number of credits in biology and
chemistry to meet the requirements.  I would call ASCP to see if you
have the requirements, it seems to me that you have the experience
needed you just need to make sure you have the credits.  That's my
opinion.  All you need is one year on the job training with a BS or BA
to sit for the HTL.  I think I'm correct on this, but if I'm not we'll
know soon enough from the replys.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Wednesday, January 13, 2010 2:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] requirements for certification

Hello.

I'm thinking about getting my HTL certification.  I've been a bench
scientist in both academia and biotech for 21 years, which has included
pre-clinical animal research and subsequent histological applications.
I've set up an histology lab and have a lot of IHC but only basic
staining (mostly H&E), as well as loads of animal tissue processing and
some human tissues.  I have a liberal arts degree (an AB) with a biology
major and a chemistry minor.  We have both a DVM and a few MD's on site,
but for pre-clinical and clinical research consult.  I would appreciate
any insight on getting certified through an online program, how many
hours is required, and what kind of mentorship is necessary.

Thank you for your insights and time!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
jander...@halozyme.com


The information transmitted in this email is confidential and is
intended only for the person(s) or entity to which it is addressed.
Delivery of this message to any person other than the intended
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applicable privilege. Any review, retransmission, dissemination or other
use of, or taking of any action in reliance upon, this information by
individuals or entities other than the intended recipient is prohibited
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RE: [Histonet] Slide marking pens

[Sherwood, Margaret]

Thanks to all who emailed me in regards to this problem.  There were many
suggestions of different pens (and pencil).  I know pencils work, but I agree
that they smear.  

I have used Secureline Markers as one person suggested, but they seemed to dry
up fast.  

One thing I am not doing is letting the ink dry thoroughly before dropping the
cassettes into formalin.  I will definitely do that and see how that works.  It
was suggested that I use fresh formalin.  That would be costly; we do change it
every 3 weeks (we don't have the volume of cassettes that histology labs run).
I will let the list know how I fare.

Peggy



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret 
Sent: Wednesday, January 13, 2010 3:50 PM
To: Mike Pence; Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens

I have a question re:  these pens.  We are a research lab that does histology
for our group.  We mark our cassettes and slides with special marking pens
(currently Statmark Pen), but we are having a problem with the ink wearing off
after processing.  We have tried marking over the info repeatly, but the ink
still comes off!  

Are these pens for this purpose?  Or can you recommend a good marking pen for
us? 
Thanks in advance.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Wednesday, January 13, 2010 3:02 PM
To: Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens

Thanks for all the rapid replies.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, January 13, 2010 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens


I order these from Staples, formerly Corporate Express.

Pens, xylene free marker, black DZ  PIL44102
Pens, xylene free marker,  greenDZ  PIL44103
Pens, xylene free marker, red   DZ  PIL44104
Pens, xylene free marker, blue  DZ  PIL44105

Hope this helps! 

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, January 13, 2010 14:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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[Histonet] requirements for certification

[Jennifer Anderson]

Hello.

I'm thinking about getting my HTL certification.  I've been a bench scientist 
in both academia and biotech for 21 years, which has included pre-clinical 
animal research and subsequent histological applications.  I've set up an 
histology lab and have a lot of IHC but only basic staining (mostly H&E), as 
well as loads of animal tissue processing and some human tissues.  I have a 
liberal arts degree (an AB) with a biology major and a chemistry minor.  We 
have both a DVM and a few MD's on site, but for pre-clinical and clinical 
research consult.  I would appreciate any insight on getting certified through 
an online program, how many hours is required, and what kind of mentorship is 
necessary.

Thank you for your insights and time!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
jander...@halozyme.com


The information transmitted in this email is confidential and is intended only 
for the person(s) or entity to which it is addressed. Delivery of this message 
to any person other than the intended recipient(s) is not intended in any way 
to waive confidentiality or any applicable privilege. Any review, 
retransmission, dissemination or other use of, or taking of any action in 
reliance upon, this information by individuals or entities other than the 
intended recipient is prohibited by Halozyme and may be in violation of 
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[Histonet] Fabric Softener sheets

[tdobersztyn]

Hello all,


When using a dryer sheet in the cryostat to reduce static- 
has anyone experienced an increased amount of artifact with fluorescein 
stained slides???
We will occasionally get a slide that has lots of fluorescent fibers all 
over the section!
I vaguely remember being told that dryer sheets are not good for labs that 
cut cryostat sections for either  muscle histochemistries /or kidney 
sections for immunofluorescent staining...

I think using a small container of alcohol placed into the cryostat will 
also help reduce static- 
These dry months kill us

Thanks in advance

Theresa
Theresa R Dobersztyn HT (ASCP)
Senior Technologist-Histology/Electron Microscopy Labs
Dept. of Pathology and Laboratory Medicine
Phone: 330-543-8279
Fax: 330-543-3226
tdobersz...@chmca.org


Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best
Workplace" award! 

*
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recipient(s). It is not intended for transmission to, or receipt by, any 
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[Histonet] new CAP guidelines for digital imaging

[Liz Chlipala]

Hello All

 

Someone mentioned about a week ago or so about new CAP guidelines for
digital imaging.  If anyone has a copy of the questions from CAP and
would be willing to share them with me I would greatly appreciate it.  

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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RE: [Histonet] Slide marking pens

[Weems, Joyce]

Remember that Surgipath is now Leica... 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Wednesday, January 13, 2010 16:24
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens

I have had very good luck with the Surgipath "Marking Pens" Cat.#01880.
I've tried many & they are the only ones that can stand up to my high pH
Heat Induced Epitope Retrieval (HIER).

Best of Luck,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

 -Original Message-
From:   histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]  On Behalf Of
Sherwood, Margaret 
Sent:   Wednesday, January 13, 2010 2:50 PM
To: Mike Pence; Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject:RE: [Histonet] Slide marking pens

I have a question re:  these pens.  We are a research lab that does
histology for our group.  We mark our cassettes and slides with special
marking pens (currently Statmark Pen), but we are having a problem with
the ink wearing off after processing.  We have tried marking over the
info repeatly, but the ink still comes off!  

Are these pens for this purpose?  Or can you recommend a good marking
pen for us? 
Thanks in advance.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, January 13, 2010 3:02 PM
To: Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens

Thanks for all the rapid replies.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, January 13, 2010 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens


I order these from Staples, formerly Corporate Express.

Pens, xylene free marker, black DZ  PIL44102
Pens, xylene free marker,  greenDZ  PIL44103
Pens, xylene free marker, red   DZ  PIL44104
Pens, xylene free marker, blue  DZ  PIL44105

Hope this helps! 

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax 





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, January 13, 2010 14:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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e-mail.


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not the intended recipient, please reply to the

FW: [Histonet] Re: Bladder Biopsies

[Lester Raff MD]

 Bob--I believe we had only minor adjustments to make in our immuno
staining when we switched from a VIP processor to a microwave.

Lester J. Raff, MD
Medical Director
UroPartners Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel 708.486.0076
Fax 708.486.0080
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Wednesday, January 13, 2010 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Bladder Biopsies

In answer to a query about microwave processing of urinary bladder
biopsy specimens Lester Raff MD (Uropartners, Winchester IL) notes:

>>We microwave process our bladder biopsies on the same cycle as
prostate biopsies.<<

Good to know this. Do the immunostains require any adjustment -
particularly the racemase stain, notorious for difficulty in adjusting
to different fixations?

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] Slide marking pens

[Breeden, Sara]

I hate pencil and refuse to use them!  They smear and blur more than any
"specialized" histo slide/cassette markers!  I just replied to a
separate post saying that as long as your NBF is not "old" (like more
than a couple days) and that you let the cassette sit for a minute or
two before you drop it into solution to make sure it's ink is completely
dry, I've not had any problems.  Besides, I think every pen manufactured
for our use has an "off day" because I've seen every brand have an
occasional glitch.  Not that this is a good thing, but it seems to be
universal.  Has something to do with the phase of the moon and what
color socks you're wearing.  May the Force be with you...

Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM  87106
505-841-2576



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Re: [Histonet] Slide marking pens

[V. Neubert]

Should've kept it for Friday's fun :-D

Anyway, I guess that there is no ink that will withstand several hours 
in both watery and organic solvants. I used Securline Lab Markers II, 
even those will faint slightly when processing. Can be scrubbed of with 
a HCl-ethanole-water solution.



> but the ink still comes off! Are these pens for this purpose?
> Or can you recommend a good marking pen for us?

yep... a pencil ;)


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[Histonet] veterinary IHC question - BVD Ab

[Jan Shivers]

Hi all,

I've been using the BVD clone 15C5 for years, but need to find another vendor 
source with reactivity to another clone.  Does anyone have experience with and 
recommendations for a commercial source of a monoclonal BVD antibody that has 
strong reactivity?  This would be for Type 2 BVD virus.

Thanks in advance,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

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RE: [Histonet] Slide marking pens

[Dawson, Glen]

I have had very good luck with the Surgipath "Marking Pens" Cat.#01880.  I've 
tried many & they are the only ones that can stand up to my high pH Heat 
Induced Epitope Retrieval (HIER).

Best of Luck,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

 -Original Message-
From:   histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu]  On Behalf Of Sherwood, 
Margaret 
Sent:   Wednesday, January 13, 2010 2:50 PM
To: Mike Pence; Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject:RE: [Histonet] Slide marking pens

I have a question re:  these pens.  We are a research lab that does histology
for our group.  We mark our cassettes and slides with special marking pens
(currently Statmark Pen), but we are having a problem with the ink wearing off
after processing.  We have tried marking over the info repeatly, but the ink
still comes off!  

Are these pens for this purpose?  Or can you recommend a good marking pen for
us? 
Thanks in advance.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Wednesday, January 13, 2010 3:02 PM
To: Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens

Thanks for all the rapid replies.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, January 13, 2010 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens


I order these from Staples, formerly Corporate Express.

Pens, xylene free marker, black DZ  PIL44102
Pens, xylene free marker,  greenDZ  PIL44103
Pens, xylene free marker, red   DZ  PIL44104
Pens, xylene free marker, blue  DZ  PIL44105

Hope this helps! 

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, January 13, 2010 14:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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sender that you have received the message in 
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RE: [Histonet] Slide marking pens

[Rathborne, Toni]

We use Tissue-Tek Marking Pencils for cassettes and slides. No smearing. We 
purchase them through Allegiance, catalog #M7321-10.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of HistoLab
Sent: Wednesday, January 13, 2010 3:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens


Gorilla Scientific sells starmark marking pens. They are resistant to
aqueous based stains, alcohols, xylene and xylene substitutes.
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[Histonet] New Job Posting - Minneapolis/St. Paul MN

[Pam Bakken]

Job Description

 Job Title:   Anatomic Pathology Scientist  
 Job ID:   19854  
 Location:  St. Paul  
 Full/Part Time:   Full-Time  
 Regular/Temporary:   Regular  
 
 
   
 Work Schedule  

Std Hrs:40 hours, 1.0 
Shift:  Days 
Shift Length:   8 hours
Weekend Coverage:  e4th 
Call Coverage:  

 Department Overview  

The laboratories at Children's Hospitals and Clinics are full service
facilities that offer a complete spectrum of laboratory techniques for
the evaluation of pediatric disorders. Laboratories are located on both
the Minneapolis and St. Paul campuses and provide services on an
inpatient and outpatient basis. Disciplines include Chemistry,
Hematology, Microbiology, Virology, Serology, Blood Bank,
Immunology/Flow Cytometry, Histology and Pathology.  

 Position Summary  

Accessions specimens in the pathology computer system and chooses
workload along with the appropriate CPT code.  Performs gross
examination of all tissue submitted to the pathology lab.  Prepares
non-gyn. cytology for staining.  Prepares tissue for microscopic
examination by a Pathologist.  Assists pathologist with autopsy exams. 
Acts as a resource for histology services.  Collaborates with the health
care team to accurately and efficiently provide laboratory services. 
Communicates results to necessary individuals. 

Qualifications 

Graduate from a Histology Technician program with HT or HTL degree. 
ASCP Certification for HT or HTL or eligible - required to obtain within
first twelve months of employment.  

 Physical Demands  

The following lists only the physical demands that have been identified
as frequent or continuous for this position.  A more detailed list is
available upon request. 
°Walking/Standing 
°Repetitive Movement - hands/wrists 
°Simple Grasping - both hands 
°Firm Grasping - Both Hands 
°Fine Manipulating - Both Hands 
°Keyboarding - both hands  

 EEO Statement  
Children's Hospitals and Clinics of Minnesota is an equal opportunity
employer and is committed to a diverse workforce.  Children's also
participates in E-Verify.  

To apply, please click on link below and enter Job ID # 19854
 
https://eprod89.pshr.childrensmn.org/psp/EXTAPP/EMPLOYEE/HRMS/c/HRS_HRS.HRS_APP_SCHJOB.GBL?FolderPath=PORTAL_ROOT_OBJECT.HC_HRS_APP_SCHJOB_GBL&IsFolder=false&IgnoreParamTempl=FolderPath%2cIsFolder


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Re: [Histonet] Slide marking pens

[Peter Carroll]

> but the ink still comes off! Are these pens for this purpose?
> Or can you recommend a good marking pen for us?

yep... a pencil ;)


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[Histonet] Slide marking pens

[HistoLab]

Gorilla Scientific sells starmark marking pens. They are resistant to
aqueous based stains, alcohols, xylene and xylene substitutes.
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RE: [Histonet] Slide marking pens

[Sherwood, Margaret]

I have a question re:  these pens.  We are a research lab that does histology
for our group.  We mark our cassettes and slides with special marking pens
(currently Statmark Pen), but we are having a problem with the ink wearing off
after processing.  We have tried marking over the info repeatly, but the ink
still comes off!  

Are these pens for this purpose?  Or can you recommend a good marking pen for
us? 
Thanks in advance.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Wednesday, January 13, 2010 3:02 PM
To: Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens

Thanks for all the rapid replies.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, January 13, 2010 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens


I order these from Staples, formerly Corporate Express.

Pens, xylene free marker, black DZ  PIL44102
Pens, xylene free marker,  greenDZ  PIL44103
Pens, xylene free marker, red   DZ  PIL44104
Pens, xylene free marker, blue  DZ  PIL44105

Hope this helps! 

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, January 13, 2010 14:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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[Histonet] job opening at UCLA

[Linke, Noelle]

Hi all,

I have an opening for a Histotech II or III in the histology lab.  For this 
position, an HTL is required for a III, HT for a II.  We are looking for 
someone with 5+ years of experience in routine histology, manual specials, 
automated special stain experience is always a help.  The hours for this 
position are 8:00pm-4:30am.

To apply, enter the job number H51527 in the 'Find Job Code' box.
https://jobs2.mednet.ucla.edu/css_external/CSSPage_BrowseJobs.ASP

Salary range for this position is $34.93-$45.18 per hour DOE for Histotech II, 
and $36.83-47.66 per hour DOE for Histotech III.  There will also be additional 
shift differential.

If you are interested, please let me know!

Thanks,
Noelle

Noёlle Linke M.S., HTL(ASCP)QIHC
Manager, Histology Services
Department of Pathology & Laboratory Medicine
David Geffen School of Medicine at UCLA
Phone: 310-825-7397
Pager: 97471
nli...@mednet.ucla.edu







  
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RE: [Histonet] Slide marking pens

[Mike Pence]

Thanks for all the rapid replies.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, January 13, 2010 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide marking pens


I order these from Staples, formerly Corporate Express.

Pens, xylene free marker, black DZ  PIL44102
Pens, xylene free marker,  greenDZ  PIL44103
Pens, xylene free marker, red   DZ  PIL44104
Pens, xylene free marker, blue  DZ  PIL44105

Hope this helps! 

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, January 13, 2010 14:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
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not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.


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RE: [Histonet] Slide marking pens

[Weems, Joyce]

I order these from Staples, formerly Corporate Express.

Pens, xylene free marker, black DZ  PIL44102
Pens, xylene free marker,  greenDZ  PIL44103
Pens, xylene free marker, red   DZ  PIL44104
Pens, xylene free marker, blue  DZ  PIL44105

Hope this helps! 

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, January 13, 2010 14:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
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not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.


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RE: [Histonet] Slide marking pens

[Diana Martinez-Longoria]

Hi,
The pathologist use pilot extra fine point permanent marker, cat# SCA-UF. The 
ink is blue. You can google the information. It has been a while since we have 
ordered that I don't remember where we got them. Hope this helps.

Diana Martinez-Longoria
Histology Laboratory/Histology Technician
El Centro Regional Medical Center
1415 Ross Ave
El Centro CA 92243
(760) 339-7267
(760)482-5365 fax

dmlongo...@ecrmc.org






Confidentiality Notice: This e-mail is for the sole use of the intended 
recipient(s) and may contain confidential and privileged information. Any 
unauthorized review, use, disclosure, or distribution is prohibited. If you are 
not the intended recipient, please contact the sender at the phone number above 
and promptly destroy this e-mail and its attachments.



From: Mike Pence
Sent: Wed 1/13/2010 11:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens


I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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[Histonet] RE: Slide marking pens

[Maria Katleba]

HI Mike,

Try BBC Biochemical at 1-800-635-4477

Cyt-O-Dot pens  item #9001   it's a four pack (one of each colour)

 green blue red and black


Call for current price

www.BBCUS.com

They are great!

Maria Katleba HT (ASCP) MS
Queen of the Valley medical Center
Pathology Dept. Mgr
707-257-4076
www.thequeen.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Wednesday, January 13, 2010 11:37 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide marking pens

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.

Mike
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Notice from St. Joseph Health System:
Please note that the information contained in this message may be privileged 
and confidential and protected from disclosure.


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[Histonet] Slide marking pens

[Mike Pence]

I am wondering if anyone can tell me where to get slide marking pens
that the Pathologist would mark an area of interest on the cover slipped
side of a slide. Cytologist use these all the time.  hey most often have
blue ink.  Thanks for any help I can get.
 
Mike
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[Histonet] H&E on frozen sections

[Jeffrey Thompson]

Hello,
 
This is a question that has probably been asked of the group many times before. 
 
I would like to know if anyone can suggest a protocol for H&E stain of 
paraformaldehyde perfused rat brain sections collected at 10 microns on a 
cryostat?
 
And, by the way, is there a method to search histonet for topics that have 
likely been covered before?
 
Thanks,
 
Jeff Thompson
 
 

University of New Mexico
Health Sciences Center
Department of Neurology
MSC11 6035
Domenici Hall / NRF 
Room 1311
1101 Yale Blvd. NE
Albuquerque, NM 87131 
USA

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RE: [Histonet] IHC edge effect

[Dimitri Scholz]

I would agree with Mesruh: there could be different reasons.
Own experience: if you stain large semithin Epon sections with toluidin
blue, edges are usually darker stained than the center. At least for this
case, we can exclude drying and be sure the different penetration of osmium
fixation is the reason.
In case of immunolabeling, however, my first move would be to check if the
sections are dried, which could cause non-specific binding or even some
specific one caused by antigen retrieval (each tissue damage could work as
an antigen retrieval!).   

Regards,
Dimitri

Dimitri Scholz, PhD, Dr. Sci.
Director of Biological Imaging
Assotiate Professor
Conway Institute 
University College Dublin (UCD)
Belfield, Dublin 4 
Ireland
Tel: +353-1 716 6736


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mesruh
turkekul
Sent: 13 January 2010 17:57
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC edge effect

Dear Colleagues,


In my opinion the edge effect problem has many different components. In each
particular case the reason/reasons for edge effect are different.
Sometimes I observe that the staining intensity gradually decreases towards
the center of the section. In this case I think this is mainly fixation
problem.
I had experienced edge effect with manual as well different automated IHC
staining systems. Sometimes adjacent sections from he same block give
different intensity of edge effect with different antibodies/protocols.
Sometimes the edge effect is due to the trauma caused during
cutting/crossing the specimen before fixation.
Sometimes due to HIER methods or drying of the sections during staining. And
different antibodies/IHC protocols have different sensitivities to all of
the above phenomena that tortures the tissue/section.
All the answers given before to that question are correct.


Regards,
Mesruh Turkekul
Memorial Sloan-Kettering Cancer Center
New York, NY 10021
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[Histonet] IHC edge effect

[mesruh turkekul]

Dear Colleagues,


In my opinion the edge effect problem has many different components. In each
particular case the reason/reasons for edge effect are different.
Sometimes I observe that the staining intensity gradually decreases towards
the center of the section. In this case I think this is mainly fixation
problem.
I had experienced edge effect with manual as well different automated IHC
staining systems. Sometimes adjacent sections from he same block give
different intensity of edge effect with different antibodies/protocols.
Sometimes the edge effect is due to the trauma caused during
cutting/crossing the specimen before fixation.
Sometimes due to HIER methods or drying of the sections during staining. And
different antibodies/IHC protocols have different sensitivities to all of
the above phenomena that tortures the tissue/section.
All the answers given before to that question are correct.


Regards,
Mesruh Turkekul
Memorial Sloan-Kettering Cancer Center
New York, NY 10021
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[Histonet] Re: Bladder Biopsies

[Robert Richmond]

In answer to a query about microwave processing of urinary bladder
biopsy specimens Lester Raff MD (Uropartners, Winchester IL) notes:

>>We microwave process our bladder biopsies on the same cycle as prostate 
>>biopsies.<<

Good to know this. Do the immunostains require any adjustment -
particularly the racemase stain, notorious for difficulty in adjusting
to different fixations?

Bob Richmond
Samurai Pathologist
Knoxville TN

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Re: [Histonet] Re: Sakura iDent

[Anne van Binsbergen]

Dear Nancy
thank you for your email - yes, I know Ghaleb well and have had a working
relationship with him for many years here in the middle east. He is indeed
trying to extract some sort of resolution from the Sakura European Head
office in Zoeterwoude - Ralph, Wim, Chris and Kees-Jan my local vendor
here in the UAE is excellent and he has witnessed my print rubbing off the
cassette face when gently brushed with xylene on gloved finger or a cotton
swab.
I must just add that it is very strange for me to be battling with a company
I have avidly supported and promoted for so many years.
I hope that we will resolve this issue and that i will add yet another
Sakura workhorse to my already large Sakura stable.
kind regards
Annie


2010/1/13 Nancy Klemme 

> Dear Annie,
>
> I am writing you from California where our Wednesday workday is beginning
> and you are preparing for your Wednesday to draw to a close.
>
> Although the majority of Sakura Finetek products are carried by all Sakura
> Finetek entities, the iDent is not one that is available from Sakura Finetek
> USA in North America.   If most Histonet subscribers are from the U.S., that
> may explain the "very little response from the histonet" that you noted.
>
> I am sorry about your cassette labeler issues and have forwarded your
> posting to the gentleman who is in charge of Sakura Finetek Middle East.
>  His name is Mr. Ghaleb Alkhaseb and I am sure he will be in contact with
> you very soon after reading it.  I do not know what Sakura contact
> information you have been using, but I know that Mr. Alkhaseb will make sure
> that you will have the most current information for a more direct company
> contact.  I do expect your iDent situation will be corrected and resolved to
> your satisfaction very soon.
>
> From 12 hours behind you, I offer my kindest regards.
>
> Nancy Klemme, HT(ASCP)
> Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA
> 800-725-8723 x7879
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van
> Binsbergen
> Sent: Wednesday, January 13, 2010 1:16 AM
> To: Denise Van Eaton
> Cc: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Sakura iDent
>
> Hi Denise
> sadly no resolution
> also sadly very little response from the histonet - except for Rene - he
> and
> i did some troubleshooting to try to see if i had missed anything - we had
> the same thoughts
> so ... i am still sitting with the iDent in its box, unused, until someone,
> somewhere can convince me that the print does not wipe off with xylene  -
> which will be hard to do seeing that i wiped it off myself and saw with my
> own eyes that xylene removes the ink!!
>
> Sakura - if you are reading this - i am not a happy camper
>
> Annie
> 2010/1/12 Denise Van Eaton 
>
> >  Hi Anne,
> >
> > We have been checking into cassette printers. Obviously, if we can bring
> > one in for a demonstration we will but I thought the iDent looked like a
> > good place to start. I noticed your problem (on the Histonet) with the
> ink
> > rubbing off after the xylene. I never saw any responses from the rest of
> the
> > Histonetters... did you resolve the problem? Assuming you (or Sakura)
> did,
> > what was the trick?
> >
> > Thank you in advance for sharing your "fix" for a potential problem.
> >
> > Denise Van Eaton HT(ASCP)
> > Litton Pathology Associates
> >
>
>
>
> --
> Anne van Binsbergen (Hope)
> Abu Dhabi
> UAE
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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AW: [Histonet] Problems with MSB stain

[Gudrun Lang]

Hi Steve,
I have noticed that the CAB-stain, which is also a trichrome-stain (like
Trichrome Gomori), shows a blue overall appearence after NBF-fixation alone.
After re-fixation in Bouin the staining shows normal red staining of
liverslides. Re-fixation is like "antigen-retrieval" and opens the right
binding sites for the dyes. 
Did you re-fix your slides in Bouin? Is there a difference in fixation of
the specimen?

Gudrun Lang


-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von
stephen.ey...@sanofi-aventis.com
Gesendet: Dienstag, 12. Jänner 2010 17:05
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Problems with MSB stain

Hi,
 
I have just encountered a problem I have never seen before and would
welcome opinions as to the cause. I work in pharma R&D and had a request
to perform MSBs on some liver slides. The results varied within the run,
with a distinct difference in the overall colour of the  slides; some
appearing blue and others red. The person performing the staining is
experienced, and our neqas scores are good. When we investigated the
problem the only difference we could identify was that the bluer slides
were stained after  40 minutes drying after being cut, whereas the
redder slides were cut the day before. This suggests that maybe slides
were incompletely dried and that this affected the staining. Comments
please.
 
Cheers
 
Steve 
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[Histonet] (Used microtome) - Thanks ...

[Massimo]

... to all those who kindly responded at my email.

Best Regards,

Massimo Tosi

=

In ricordo di "Nice":

"E Argo, che aveva visto Odisseo dopo vent’anni,

fu preso dal Fato della nera morte."

Omero, Odissea, XVII, 290-327



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Re: RE: [Histonet] Problems with MSB stain

[John Kiernan]

The unduly blue sections are all from Animals 12, 13 and 14. Anything different 
about these specimens? The blue and red sections surely must look different 
under the microscope.
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: stephen.ey...@sanofi-aventis.com
Date: Wednesday, January 13, 2010 10:12
Subject: RE: [Histonet] Problems with MSB stain
To: jkier...@uwo.ca


> Hi John,
>  
> Many thanks for the rapid response. The slides look fine down the microscope 
> except for a few that macroscopically look blue. However because the MSBs 
> will be sent away for evaluation, we want to send a consistent set of slides. 
> I attach a picture to show the problem. I can also scan a slide if you 
> prefer, but the image size is large. The paper was very helpful.
>  
> Cheers
>  
> Steve
> 



> From: John Kiernan [mailto:jkier...@uwo.ca] 
> Sent: Wednesday, January 13, 2010 6:23 AM
> To: Eyres, Stephen R&D/GB
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Problems with MSB stain
> 

> What do the slides look like under the microscope? In a normal liver there 
> shouldn't be much collagen (blue) and there should be plenty of cytoplasm 
> (red). If the preceding nuclear stain was too strong, that could put unwanted 
> bluish shades in the cytoplasm. 
>  
> Trichrome methods aren't at their best after formaldehyde fixation; the 
> colours can be improved by soaking the hydrated sections in saturated aqueous 
> picric acid for 30 min or so at 55-60C before staining. Bouin's solution is 
> also used for this purpose, and it is said that the same effect can be 
> achieved with citrate buffer pH4 or with 1% iodine in 2% aqueous KI.  See Yu 
> Y, Chapman CM (2003) Masson trichrome stain: postfixation substitutes. J. 
> Histotechnol. 26: 131-134. I've not yet tried these alternatives to picric 
> acid; has anyone else?
>  
> Lendrum's 1962 paper, which includes colour photos, includes many technical 
> tips. More than 40 years ago a med lab technician in Birmingham, UK, 
> suggested this method to me as a way to impart different colours to gliosis 
> (red) and collagenous scarring (blue) in sections of the brains of frogs and 
> other animals in which I'd made surgical lesions dorsal to the optic chiasma. 
> The MSB method showed these changes very well. I recall that we had to buy 
> about 500 grams of brilliant crystal scarlet 6R because it was then cheaper 
> as an an article of commerce than as a bioogical stain.
>  
> I have appended a PDF of Lendrum et al 1962.  This won't  gwt through to all 
> Histonetters, but it might make it to stephen.ey...@sanofi-aventis.com. 
>  
>  
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> - Original Message -
> From: stephen.ey...@sanofi-aventis.com
> Date: Tuesday, January 12, 2010 11:06
> Subject: [Histonet] Problems with MSB stain
> To: histonet@lists.utsouthwestern.edu
> 
> > Hi,
> >  
> > I have just encountered a problem I have never seen before and would
> > welcome opinions as to the cause. I work in pharma R&D and had a 
> > requestto perform MSBs on some liver slides. The results varied 
> > within the run,
> > with a distinct difference in the overall colour of the  
> > slides; some
> > appearing blue and others red. The person performing the 
> > staining is
> > experienced, and our neqas scores are good. When we investigated the
> > problem the only difference we could identify was that the bluer 
> > slideswere stained after  40 minutes drying after being 
> > cut, whereas the
> > redder slides were cut the day before. This suggests that maybe slides
> > were incompletely dried and that this affected the staining. Comments
> > please.
> >  
> > Cheers
> >  
> > Steve 
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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RE: [Histonet] Re: Sakura iDent

[Nancy Klemme]

Dear Annie,

I am writing you from California where our Wednesday workday is beginning and 
you are preparing for your Wednesday to draw to a close.

Although the majority of Sakura Finetek products are carried by all Sakura 
Finetek entities, the iDent is not one that is available from Sakura Finetek 
USA in North America.   If most Histonet subscribers are from the U.S., that 
may explain the "very little response from the histonet" that you noted.

I am sorry about your cassette labeler issues and have forwarded your posting 
to the gentleman who is in charge of Sakura Finetek Middle East.  His name is 
Mr. Ghaleb Alkhaseb and I am sure he will be in contact with you very soon 
after reading it.  I do not know what Sakura contact information you have been 
using, but I know that Mr. Alkhaseb will make sure that you will have the most 
current information for a more direct company contact.  I do expect your iDent 
situation will be corrected and resolved to your satisfaction very soon.

>From 12 hours behind you, I offer my kindest regards.

Nancy Klemme, HT(ASCP)
Edu. Svcs. Dir. - Sakura Finetek USA, Inc. - Torrance, CA
800-725-8723 x7879

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van 
Binsbergen
Sent: Wednesday, January 13, 2010 1:16 AM
To: Denise Van Eaton
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Sakura iDent

Hi Denise
sadly no resolution
also sadly very little response from the histonet - except for Rene - he and
i did some troubleshooting to try to see if i had missed anything - we had
the same thoughts
so ... i am still sitting with the iDent in its box, unused, until someone,
somewhere can convince me that the print does not wipe off with xylene  -
which will be hard to do seeing that i wiped it off myself and saw with my
own eyes that xylene removes the ink!!

Sakura - if you are reading this - i am not a happy camper

Annie
2010/1/12 Denise Van Eaton 

>  Hi Anne,
>
> We have been checking into cassette printers. Obviously, if we can bring
> one in for a demonstration we will but I thought the iDent looked like a
> good place to start. I noticed your problem (on the Histonet) with the ink
> rubbing off after the xylene. I never saw any responses from the rest of the
> Histonetters... did you resolve the problem? Assuming you (or Sakura) did,
> what was the trick?
>
> Thank you in advance for sharing your "fix" for a potential problem.
>
> Denise Van Eaton HT(ASCP)
> Litton Pathology Associates
>



--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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[Histonet] Part-time Flex Histologist position

[Fallak, Alice]

We have a part-time/Flex Histologist position open at United Hospital
and Medical Center in Kenosha,Wi.
Anyone interested can contact our Human Resource Dept. at (262)656-2116.
 
 
Alice Fallak
Histology coordinator
United Hosp. & Med. Center
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Re: [Histonet] Eliminating the edge effect in IHC/IF

[histology]

Hi Karen/Histonet,
This is a problem we have been working on for some time with our IHC.  We
have ruled out fixation as the source of the problem, and we are in Oregon
so we don't believe we are having a drying out issue.  I have also tried
different detection kits with both having this artifact.  Recently we were
wondering if it could be a problem with our blower. Is it possible that it
is blowing too hard, or that the rinses are too hard? We have tried hand
staining twice, once with perfect results, once with the same artifact. 
Any additional advice you can give will be greatly appreciated.
Thank you, once again,
Katelin Lester, HTL(ASCP)
MedSurg Pathology Associates, Inc.
(formerly Cutting Edge Histology)
(503)443-2157

> Hi,
> I have a question for the generous input. When I do the IHC or IF, it
> seems very common that the intensity of the edge area of the tissue is
> always stronger than the central tissue part. Is it possible to
> eliminate this and make the staining evenly distributed around the whole
> tissue section?
>
> Your kind help is greatly appreciated,
>
>
> Thanks in advance,
>
> Best,
> Karen
>
> Karen Cai
> Research Scientist
> Prosci Incorporated
> (858) 513-2638 x 204
> (858) 513-2692 Fax
>   www.prosci-inc.com
>
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>




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[Histonet] Re: Bladder Biopsies

[Lester Raff MD]

We microwave process our bladder biopsies on the same cycle as prostate
biopsies.

 

Lester J. Raff, MD

Medical Director

UroPartners Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel 708.486.0076

Fax 708.486.0080

 

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[Histonet] Bladder Biopsies

[S R]

Hello Everyone!

I have a couple of questions.  I work for a urology group.  As of right now i 
only work with prostate bx's.  They want to add Bladder bx's into the mix.  As 
of right now i microwave process them.  I was wondering if anyone eles does 
this and if you use a different procedure than you would use for processing the 
prostate biopsies, and would anyone be willing to share?

Thanks in Advanced

Sammy

 
  
_
Your E-mail and More On-the-Go. Get Windows Live Hotmail Free.
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AW: [Histonet] Eliminating the edge effect in IHC/IF

[Gudrun Lang]

My favourite for intense specific staining at the edges vs. faint specific
staining in the center is poor fixation.
I think drying artefacts or crush artefacts would lead rather to unspecific
background staining.

Gudrun Lang

Histolab, Akh Linz, Austria

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Karen Cai
Gesendet: Montag, 11. Jänner 2010 20:01
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Eliminating the edge effect in IHC/IF

Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?
 
Your kind help is greatly appreciated,
 
 
Thanks in advance,
 
Best,
Karen
 
Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
  www.prosci-inc.com
 
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RE: [Histonet] Re: Sakura iDent

[Cynthia Pyse]

Anne
You might want to try going up the Sakura chain of command. I had trouble
with a coverslipper, after contacting the regional manager it was amazing
the service it received. Good luck.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van
Binsbergen
Sent: Wednesday, January 13, 2010 4:16 AM
To: Denise Van Eaton
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Sakura iDent

Hi Denise
sadly no resolution
also sadly very little response from the histonet - except for Rene - he and
i did some troubleshooting to try to see if i had missed anything - we had
the same thoughts
so ... i am still sitting with the iDent in its box, unused, until someone,
somewhere can convince me that the print does not wipe off with xylene  -
which will be hard to do seeing that i wiped it off myself and saw with my
own eyes that xylene removes the ink!!

Sakura - if you are reading this - i am not a happy camper

Annie
2010/1/12 Denise Van Eaton 

>  Hi Anne,
>
> We have been checking into cassette printers. Obviously, if we can bring
> one in for a demonstration we will but I thought the iDent looked like a
> good place to start. I noticed your problem (on the Histonet) with the ink
> rubbing off after the xylene. I never saw any responses from the rest of
the
> Histonetters... did you resolve the problem? Assuming you (or Sakura) did,
> what was the trick?
>
> Thank you in advance for sharing your "fix" for a potential problem.
>
> Denise Van Eaton HT(ASCP)
> Litton Pathology Associates
>



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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[Histonet] Looking for Florida licensed Histologist

[Kaye Ryan]

North Florida Dermatology Associates located in Jacksonville, Florida is
looking for a full time histologist.  Must be eligible for Florida State
Technologist license.  The lab is a small private lab with good benefits
and minimal stress.  Responsibilities include routine histology and some
IHC techniques.  If interested, please contact me or our Personnel
Director at 904-398-0547, ext. 1106.

 

Kaye Ryan

North Florida Dermatology Associates

Histology Lab Supervisor

(904) 398-0547 Ext. 2004

 

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[Histonet] Re: Sakura iDent

[Anne van Binsbergen]

Hi Denise
sadly no resolution
also sadly very little response from the histonet - except for Rene - he and
i did some troubleshooting to try to see if i had missed anything - we had
the same thoughts
so ... i am still sitting with the iDent in its box, unused, until someone,
somewhere can convince me that the print does not wipe off with xylene  -
which will be hard to do seeing that i wiped it off myself and saw with my
own eyes that xylene removes the ink!!

Sakura - if you are reading this - i am not a happy camper

Annie
2010/1/12 Denise Van Eaton 

>  Hi Anne,
>
> We have been checking into cassette printers. Obviously, if we can bring
> one in for a demonstration we will but I thought the iDent looked like a
> good place to start. I noticed your problem (on the Histonet) with the ink
> rubbing off after the xylene. I never saw any responses from the rest of the
> Histonetters... did you resolve the problem? Assuming you (or Sakura) did,
> what was the trick?
>
> Thank you in advance for sharing your "fix" for a potential problem.
>
> Denise Van Eaton HT(ASCP)
> Litton Pathology Associates
>



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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