Re: RE: Re: [Histonet] Eliminating the edge effect in IHC/IF

[TF]

staining was performed on floating sections...and thus the incubation can reach 
the both sides of sections
normally we use 12/24/48 well plate, add 1 ml/0.5ml/0.2ml antibody diluted 
solution and shake 

widely used for brain section staining...

the wash will be better, in the edge parts of sections


2010-01-15 



TF 



发件人： Karen Cai 
发送时间： 2010-01-15  03:29:56 
收件人： ti...@foxmail.com; 'Rene J Buesa'; 'histonet' 
抄送： 
主题： RE: Re: [Histonet] Eliminating the edge effect in IHC/IF 
 
Hi, 
Thank you very much for your input. Could you describe more with the floating 
section method?  I never did it before.
 
Best,
Karen
 
-Original Message-
From: TF [mailto:ti...@foxmail.com] 
Sent: Wednesday, January 13, 2010 6:11 PM
To: Rene J Buesa; histonet; Karen Cai
Subject: Re: Re: [Histonet] Eliminating the edge effect in IHC/IF
 
Hi all
i believe it is due to the wash + antibody accumulation problem happened 
between the edge of section and the adhesive slide.
 
Even you have circled the section with IHC pen(DAKO), and flattened all 
soultions evenly, it improved but did not disappear
 
if you stain the section using "floating section" method, no edge effect now.
 
 
2010-01-14 



TF 



发件人： Rene J Buesa 
发送时间： 2010-01-12  13:30:49 
收件人： histonet; Karen Cai 
抄送： 
主题： Re: [Histonet] Eliminating the edge effect in IHC/IF 
Usually that is the result of incomplete fixation. Check your fixation protocol.
René J.
--- On Mon, 1/11/10, Karen Cai  wrote:
From: Karen Cai 
Subject: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 2:00 PM
Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?
Your kind help is greatly appreciated,
Thanks in advance,
Best,
Karen
Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
 www.prosci-inc.com
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RE: [Histonet] Thermometer holder used with water bath

[WILLIAM DESALVO]

I have not used the suction cup , but do have an inexpensive solution.

Rubber washers. Select a two small rubber washers w/ an inside diameter 
slightly larger than the outside diameter of your thermometer and the outside 
diameter of the washer that provides approximately 3/4 inch distance from the 
inside opening. Remove a small portion of the edge to create a flattened 
section, slide the washers on each end and place in water bath. This method 
will elevate the thermometer from the bottom surface and the flattened edge 
will allow you to position so that you can easily read the thermometer. Simple, 
inexpensive and easily replaced. You should be able to find a washer to fit any 
size thermometer.

William DeSalvo, B.S., HTL(ASCP)





> Date: Thu, 14 Jan 2010 19:07:04 +
> From: ethomas3...@comcast.net
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Thermometer holder used with water bath
> 
> 
> 
> Hi all, 
> 
> 
> 
> I like to know if anyone recall using in the past or present a small rubber 
> suction cup that holds a thermometer approx. 4 in length at the bottom of the 
> glass insert of a water bath. This accessory is approximately 1 inch in 
> diameter and the thermometer slides across it. 
> 
> If you know the name and/or vendor, please let me know. 
> 
> 
> 
> Thanks 
> 
> Gina 
> 
> 
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[Histonet] Unsubscribe

[Sebastián D. Calligaris]

Unsubscribe



Sebastián D. Calligaris, PhD
Investigador
Instituto de Ciencias
Facultad de Medicina
Clínica alemana-Universidad del Desarrollo




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[Histonet] Dermpath Consultants

[Andrew Burgeson]

In response to a question posed some time ago, if you are
looking for a consultant to build a DERMPATH LAB, contact
Andrew Burgeson, HTL @Buckeye Dermatology inc.
Dermatopathologist/dermatologists and HISTOTECHS giving
advice and guidance, as opposed to venture capitalists and
non-medical third parties who won't be able to teach you the
nuances of the trade. Contact buckeyed...@gmail.com or 513
680 1809. 

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Re: [Histonet] Unsuscribe

[Emily Sours]

MY BRAIN JUST EXPLODED.
USE.
THE.
LINK.
AT.
THE.
BOTTOM.
OF.
HISTONET.
EMAILS.

Emily

...the thrill of being close to that hidden knowledge. That's the way I feel
when I read Nabokov. Encrypted within his words, encoded indecipherably,
ambiguously, is the equivalent of the secret of lightning. Something akin to
the secret code of higher human consciousness, the DNA, the genome of
genius.
-Ron Rosenbaum


On Thu, Jan 14, 2010 at 3:56 PM, Lorena Benedetti
wrote:

> Unsuscribe
>
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Re: [Histonet] Unsubscribe

[Emily Sours]

By Zeus's beard, I cannot imagine why ANYONE is still writing unsubscribe to
the list.
USE THIS LINK.
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
NOTE THAT IT IS AT THE BOTTOM OF EVERY EMAIL YOU SEND.  EVEN THE ONES WITH
THE WORD "unsubscribe"

Emily

...the thrill of being close to that hidden knowledge. That's the way I feel
when I read Nabokov. Encrypted within his words, encoded indecipherably,
ambiguously, is the equivalent of the secret of lightning. Something akin to
the secret code of higher human consciousness, the DNA, the genome of
genius.
-Ron Rosenbaum


On Thu, Jan 14, 2010 at 3:52 PM, Martinez, Angela wrote:

> Unsubscribe
>
>
>
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[Histonet] Unsuscribe

[Lorena Benedetti]

Unsuscribe

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[Histonet] Unsubscribe

[Martinez, Angela]

Unsubscribe 

 

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[Histonet] CD15 clone

[Taylor, Jean]

Hi everyone,

I'd like to hear what clone and company labs are using for their CD15 antibody.

Thanks!

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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Re: [Histonet] Fabric Softener sheets

[Pat Laurie]

In my lab we have a couple of Techs that use the spray Static Guard, and
sprays the microtome occasionally.  I am not sure how well it works.  It
might freeze inside of the cryostat...

On Wed, Jan 13, 2010 at 1:34 PM,  wrote:

> Hello all,
>
>
> When using a dryer sheet in the cryostat to reduce static-
> has anyone experienced an increased amount of artifact with fluorescein
> stained slides???
> We will occasionally get a slide that has lots of fluorescent fibers all
> over the section!
> I vaguely remember being told that dryer sheets are not good for labs that
> cut cryostat sections for either  muscle histochemistries /or kidney
> sections for immunofluorescent staining...
>
> I think using a small container of alcohol placed into the cryostat will
> also help reduce static-
> These dry months kill us
>
> Thanks in advance
>
> Theresa
> Theresa R Dobersztyn HT (ASCP)
> Senior Technologist-Histology/Electron Microscopy Labs
> Dept. of Pathology and Laboratory Medicine
> Phone: 330-543-8279
> Fax: 330-543-3226
> tdobersz...@chmca.org
>
>
> Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best
> Workplace" award!
>
> *
> This electronic mail transmission, including any attached files, may
> contain confidential and/or privileged information for the sole use of the
> intended recipient(s). It is not intended for transmission to, or receipt
> by, any unauthorized parties. Any review, use, distribution, dissemination,
> downloading, copying or disclosure by others is strictly
> prohibited. If you are not the intended recipient (or authorized to receive
> information for the intended recipient), you are hereby notified that you
> have received this transmission in error. If you have received this
> transmission in error, please delete it, as well as any copies, from your
> system without copying it, and notify the sender by reply e-mail.
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plau...@cellnetix.com
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RE: Re: [Histonet] Eliminating the edge effect in IHC/IF

[Karen Cai]

Hi, 
Thank you very much for your input. Could you describe more with the
floating section method?  I never did it before.
 
Best,
Karen
 
-Original Message-
From: TF [mailto:ti...@foxmail.com] 
Sent: Wednesday, January 13, 2010 6:11 PM
To: Rene J Buesa; histonet; Karen Cai
Subject: Re: Re: [Histonet] Eliminating the edge effect in IHC/IF
 
Hi all
i believe it is due to the wash + antibody accumulation problem happened
between the edge of section and the adhesive slide.
 
Even you have circled the section with IHC pen(DAKO), and flattened all
soultions evenly, it improved but did not disappear
 
if you stain the section using "floating section" method, no edge effect
now.
 
 
2010-01-14 
  _  

TF 
  _  

发件人： Rene J Buesa 
发送时间： 2010-01-12  13:30:49 
收件人： histonet; Karen Cai 
抄送： 
主题： Re: [Histonet] Eliminating the edge effect in IHC/IF 
Usually that is the result of incomplete fixation. Check your fixation
protocol.
René J.
--- On Mon, 1/11/10, Karen Cai  wrote:
From: Karen Cai 
Subject: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 2:00 PM
Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?
Your kind help is greatly appreciated,
Thanks in advance,
Best,
Karen
Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
 www.prosci-inc.com
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[Histonet] Thermometer holder used with water bath

[ethomas3224]

Hi all, 



I like to know if anyone recall using in the past or present a small rubber 
suction cup that holds a thermometer approx. 4 in length at the bottom of the 
glass insert of a water bath. This accessory is approximately 1 inch in 
diameter and the thermometer slides across it. 

If you know the name and/or vendor, please let me know. 



Thanks 

Gina 


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[Histonet] Automated special stainers

[kristen arvidson]

Which automated Special Stainer does everyone like best?  Does any of them do a 
Fontana stain?
Thanks



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[Histonet] Jennifer Hofecker

[Laurie Colbert]

Hi,

 

Does someone have Jennifer's phone number?  I have a possible CJD case.

 

Laurie Colbert

Huntington Hospital

Pasadena ca

(626) 397-8620

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[Histonet] Leica CM 1850 UV cryostat

[Kathy M. Gorham]

Good Morning Histonetters,  We have a Leica CM 1850 UV cryostat and I
was wondering how often others are using the UV to decontaminate the
cryostat.  When we bought this I was told to use it only when we
suspected TB or the like.  BUT we are to treat ALL human tissue as if we
knew for certain that it was infectious right?  So with this in mind are
others using the UV light after every frozen section.  We are a small
lab doing maybe 10 FS a month. I need to write a procedure on how often
this should be done so wanted your option. We do disinfect the cryostat
with 70% Alc.  Have a great day,  Kathy Gorham, H.T.


GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS

GRH Mission
We will ensure ACCESS to superior QUALITY, affordable health care for all those 
in need of our services, including underserved populations within our 
communities.


GRH Confidentiality Notice
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and should be protected against viewing by unauthorized persons. The information
herein may have been disclosed from records whose confidentiality is protected
by Federal and State Law. Federal regulations prohibit further distribution or
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transmission in error, please notify the sender immediately to arrange for 
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RE: [Histonet] Histology Job Opportunities

[Dimitri Scholz]

Hi KC Carpenter,
I am not looking for a Histology Supervisor position (at least not for now), 
but could be helpful for you and your clients as a consultant.
We collected a lot of expertise here in Dublin and offer a Master Course in 
Imaging starting from September 2010. 
Earlier this spring, we will offer some stand-alone teaching modules in 
advanced microscopy methods for PhD students and technicians.
Kind regards,
Dimitri

Dimitri Scholz, PhD, Dr. Sci.
Director of Biological Imaging
Associate Professor
Conway Institute 
University College Dublin (UCD)
Belfield, Dublin 4 
Ireland
Tel: +353-1 716 6736


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of K.C. Carpenter
Sent: 14 January 2010 14:04
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Job Opportunities




Dear Histonet Subscribers, 


 


I am a one of the founders of a Healthcare Recruiting firm that specializes in 
placing Lab Professionals. We work exclusively on permanent positions and have 
clients across the country. We are completely free of charge to candidates and 
are currently working on numerous Histology positions. Our clients often assist 
with relocation expenses. If you're looking for a new job please then please 
email a resume to k...@ka-recruiting and let me know when you're available to 
talk. 


 


Below is a list of some of the other Histology opportunities we are currently 
working on. 


  


Histotechs/Cytotechs:



 


Connecticut- Histology Operations Manager



Southern CA - Histology Supervisor



New York City - Surgical Pathology and Histology Supervisor



New York City - Histotech 3rd shift



Las Vegas, NV - Histotech 3rd shift



Central Georgia - Histotech 1st shift



Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor)



Long Island, NY - Cytotech



New York City - Cytotech



Pennsylvania - Cytology Supervisor



Connecticut  Cytotech


 


If you're interested in learning more about any of these opportunities then 
please email me a resume and let me know how best to get in touch with you.  
If none of these are a fit please let me know what you'd be interested in and 
where you're looking so I can tailor a search for you.  With the New Year upon 
us many of our clients have fresh hiring budgets and will be looking to add 
people over the next several months. We work on positions at all levels and 
cover the entire US. To view some additional opportunities please visit our 
website at www.ka-recruiting.com .  



 


Sincerely












KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



k...@ka-recruiting.com



www.ka-recruiting.com
  




 





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[Histonet] RE: requirements for certification

[Lecorchick, William]

Histotechnician, HT(ASCP)

Application Fee: $185

To be eligible for this examination category, an applicant must satisfy the 
requirements of at least one of the following routes:

Route 1: Successful completion of a NAACLS accredited Histotechnician program 
within the last 5 years prior to the date of application for examination; or

Route 2: At least 60 semester hours (90 quarter hours) of academic credit from 
a regionally accredited college/university, with a combination of 12 semester 
hours (18 quarter hours) of biology and chemistry, or Associate degree from a 
regionally accredited college/university, with a combination of 12 semester 
hours (18 quarter hours) of biology and chemistry, AND one year full time 
acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint 
Commission (JCAHO) accredited laboratory within the last ten years under the 
supervision of a pathologist (certified by the American board of Pathology in 
Anatomic Pathology), or an appropriately board certified medical scientist.

Clinical Laboratory Experience
To fulfill the experience requirement for the Histotechnologist examination, 
you must have experience, within the last ten years, in the following areas: 
Fixation, Microtomy, Processing, Staining



Histotechnologist, HTL(ASCP)

Application Fee: $210

To be eligible for this examination category, an applicant must satisfy the 
requirements of at least one of the following routes:

Route 1: Baccalaureate degree from a regionally accredited college/university 
with a combination of 30 semester hours (45 quarter hours) of biology and 
chemistry AND successful completion of a NAACLS accredited Histotechnician or 
Histotechnology program within the last 5 years; or

Route 2: Baccalaureate degree from a regionally accredited college/university 
with a combination of 30 semester hours (45 quarter hours) of biology and 
chemistry AND one year full time acceptable experience in a histopathology 
laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO) accredited 
laboratory within the last ten years. This year of experience must be under the 
supervision of a pathologist (certified by the American Board of Pathology in 
Anatomic Pathology) or an appropriately board certified medical scientist.

Clinical Laboratory Experience
To fulfill the experience requirement for the Histotechnologist examination, 
you must have experience, within the last ten years, in the following areas: 
Fixation, Microtomy, Processing, Staining

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RE: [Histonet] new CAP guidelines for digital imaging

[Rathborne, Toni]

Might just as well reply to all.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Terri
Braud
Sent: Thursday, January 14, 2010 9:22 AM
To: Horn, Hazel V; Liz Chlipala; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new CAP guidelines for digital imaging


Me, three

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital and Medical Center
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3676 phone
(215) 938-3689 fax


-Original Message-
Hazel V
Sent: Thursday, January 14, 2010 09:02
To: Liz Chlipala; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new CAP guidelines for digital imaging


I am interested in the information as well.  Thanks.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 

 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Wednesday, January 13, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] new CAP guidelines for digital imaging

Hello All

 

Someone mentioned about a week ago or so about new CAP guidelines for
digital imaging.  If anyone has a copy of the questions from CAP and
would be willing to share them with me I would greatly appreciate it.  

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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RE: [Histonet] new CAP guidelines for digital imaging

[Terri Braud]

Me, three

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital and Medical Center
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3676 phone
(215) 938-3689 fax


-Original Message-
Hazel V
Sent: Thursday, January 14, 2010 09:02
To: Liz Chlipala; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new CAP guidelines for digital imaging


I am interested in the information as well.  Thanks.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 

 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Wednesday, January 13, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] new CAP guidelines for digital imaging

Hello All

 

Someone mentioned about a week ago or so about new CAP guidelines for
digital imaging.  If anyone has a copy of the questions from CAP and
would be willing to share them with me I would greatly appreciate it.  

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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[Histonet] Histology Job Opportunities

[K.C. Carpenter]

Dear Histonet Subscribers, 


 


I am a one of the founders of a Healthcare Recruiting firm that specializes in 
placing Lab Professionals. We work exclusively on permanent positions and have 
clients across the country. We are completely free of charge to candidates and 
are currently working on numerous Histology positions. Our clients often assist 
with relocation expenses. If you're looking for a new job please then please 
email a resume to k...@ka-recruiting and let me know when you're available to 
talk. 


 


Below is a list of some of the other Histology opportunities we are currently 
working on. 


  


Histotechs/Cytotechs:



 


Connecticut- Histology Operations Manager



Southern CA - Histology Supervisor



New York City - Surgical Pathology and Histology Supervisor



New York City - Histotech 3rd shift



Las Vegas, NV - Histotech 3rd shift



Central Georgia - Histotech 1st shift



Oklahoma - Histotech 1st shift (with opportunity to be promoted to supervisor)



Long Island, NY - Cytotech



New York City - Cytotech



Pennsylvania - Cytology Supervisor



Connecticut – Cytotech


 


If you're interested in learning more about any of these opportunities then 
please email me a resume and let me know how best to get in touch with you.  
If none of these are a fit please let me know what you'd be interested in and 
where you're looking so I can tailor a search for you.  With the New Year upon 
us many of our clients have fresh hiring budgets and will be looking to add 
people over the next several months. We work on positions at all levels and 
cover the entire US. To view some additional opportunities please visit our 
website at www.ka-recruiting.com
.  



 


Sincerely












KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



k...@ka-recruiting.com



www.ka-recruiting.com
  




 



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RE: [Histonet] new CAP guidelines for digital imaging

[Horn, Hazel V]

I am interested in the information as well.  Thanks.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax501.364.3155
 
visit us on the web at:www.archildrens.org
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Wednesday, January 13, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] new CAP guidelines for digital imaging

Hello All

 

Someone mentioned about a week ago or so about new CAP guidelines for
digital imaging.  If anyone has a copy of the questions from CAP and
would be willing to share them with me I would greatly appreciate it.  

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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Re:[Histonet] veterinary IHC question - BVD Ab

[Kathleen Jones]

Hello Jan

I have been trying to find a reliable source for a decent BVD antibody
for a long time now with no success. Idexx will supply a good antibody
for certain research projects, but not for any diagnostic work. Please
keep us informed if you can find a supplier. Good luck!

Kathy


Kathleen Jones
Research Technician
Pathology/Microbiology
AVC - UPEI
(902)566-0595


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