[Histonet] Golder's trichrome stain

[Liang, Yan]

We have trouble to stain bone paraffin section with Golder's trichrome
stain. Our lab use stain for over ten years for plastic and paraffin
section on bone. The stain is stable. Only this time, our some
mineralized bone of the section was stained with red and green color
mix. We checked reagents and changed every reagent, prepared new
solutions, still the same. Does anyone have experience with the red bone
stain?

 

Thank you

Yan Liang MD PhD

MDS Pharma 


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[Histonet] RE: Experience with Tissue Tek VIP6 processor

[Nancy Klemme]

Hello, Dr. Rabe:

I working on my 40th year as a histologist and remember hearing or reading of 
tissue processing by going from isopropanol directly into the paraffin step 
that had taken place in the 1950's or '60's.  I'm not sending this as a 
declaration of my age, but, rather as a reference that xylene-free processing 
has been around for a long, long time, even though most of us used a clearing 
step between the dehydrant and the paraffin steps.  ANY tissue processor (human 
or mechanical) can be used for this technique because the key elements are the 
chemicals that the tissues are being exposed to.

Because attachments cannot be included, as a private eMail, I will send you a 
White Paper that Sakura published last year using this technique with the VIP6 
that you're considering.  Anyone reading this may obtain it from your Sakura 
Sales Manager or simply send me a return eMail.  I'll also request to have it 
placed on our company web-site.  As with any change in specimen handling 
procedure, you will want to document your validation of this technical change.

Whatever instrument you ultimately choose to purchase, I send my kind regards.
Nancy Klemme
Edu. Svcs. Dir. / Sakura Finetek USA, Inc.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe
Sent: Friday, January 15, 2010 8:05 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Experience with Tissue Tek VIP6 processor

Our lab is in the market for a new processor.  Does anyone have experience with 
the Tissue Tek
VIP 6?  And does anyone have experience using it xylene-free?  Our histology 
supervisor would
love to talk with you.

Kim Rabe, M.D.
North Central Pathology, P.A.

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[Histonet] Leica 6 xylene free

[Madary, Joseph]

Do you mean clearant free or xylene free?  I use Hemo de and so far it
has been fine. 

 

Nick Madary, HT/HTL(ASCP)QIHC

Histology Mgr, Medimmune

301.398.6360(lab), 4745(vm),9745(fax)

 

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If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose.  Thank you for your cooperation."

 

 




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[Histonet] RE: Dispensing stains from gallon jugs

[gayle callis]

You wrote:  

We have had continuing problems when pouring some stain reagents out of the
gallon jugs in which they are sold.  We have all ruined shoes and scrubs
from the splashing as the stain comes out of the bottle.  

 



 

Not sure if you can find a spout adaptor.  The ones I have used never work
very well, still drip and are messy.  You did not say if you use a funnel to
help direct the reagent into the stain containers.  Be sure it is a liquid
funnel, one with tapered end as compared to a powder funnel (wide end for
introducing powders/solids to containers).  Liquid funnel should direct the
stain without extra splashing, and introduce the stain to the side of the
funnel..  You may want or might have a ring stand to hold the funnel.  Some
funnels have extra long ends.  

 

Also, there is an old trick taught in chemistry labs - use a very large
serological pipette (25 or 50 ml size) and pour your stain so it flows down
the pipette.  Directing the flow into the container prevents splashing and
spills.A plastic turkey baster found in any grocery store with a large
diameter tube (remove the bulb) will also do the job - then wash and reuse. 

 

Gayle M. Callis

HTL/HT/MT(ASCP)

Bozeman MT 

 

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[Histonet] Re: VIP6 Processor

[gayle callis]

We have just replaced a VIP 1000 K series with a VIP6 and are absolutely
delighted with the instrument.  We had to shorten our routine tissue
processing when over dehydration occurred, but this was due to the machine
being more efficient compared to the older model.  A minor adjustment only. 

 

We have not been disappointed, plus it is easy to operate, and has many
excellent features (keeps records, 50 processing schedules possible).  The
latter has allowed each research laboratory to have their own processing
schedule to fit their exact needs - custom processing at its best. 

 

Gayle M. Callis

HTL/HT/MT(ASCP)

Bozeman MT  

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[Histonet] need tech with experience

[Histonet]

beautiful southern california with all our pollution and traffic has a 
potential opening for an experience tech at a high volume fast paced reference 
lab. we focus highly on quality and consistency and need to have people with 
the ability to cut and embed fast and still maintain a high level of quality. 
start time is approx. midnight, maybe as late as 2 a.m. we are currently 
working mon. through sat. so we might need a tue. through sat. schedule.
reply if interested,
curt


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[Histonet] Re: Mitochodrial IHC marker in FFPE skeletal muscle

[Hobbs, Carl]

I have not yet found an anti mitochondrial Ab that works in mouse FFPE tissues, 
so I am very interested.
However, the protein that Chemicon's MAB1273 is directed against survivesit 
has worked very well for me in human FFPE sections after Citric acid HIER ( see 
here for images: http://www.immunoportal.com/index.php )
At light microscopic level, no mitochondrial architecture is visible: rather, a 
series of dots are seen.

STAT3 in mitochondria?

Good luck.
Sincerely, 
carl


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Re: [Histonet] MGMT mutation analysis

[Kim Tournear]

Contact Gwen at Lab Corp in Phoenix, AZ at 602454-8088...she should be able to 
help



~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson,  AZ
~Don't let your life end before it begins~
OU Rocks

--- On Fri, 1/15/10, Rathborne, Toni  wrote:


From: Rathborne, Toni 
Subject: [Histonet] MGMT mutation analysis
To: histonet@lists.utsouthwestern.edu
Date: Friday, January 15, 2010, 9:42 AM



We have a physician request for an MGMT mutation analysis on a paraffin block 
of a lung resection, previously diagnosed as malignant melanoma. I'm looking 
for a lab that performs this test. Reference labs feel free to reply.

Toni Rathborne
Pathology Supervisor
Somerset Medical Center
110 Rehill Ave.
Somerville,NJ 08876
908-595-2367



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[Histonet] Poorly Designed Stain Bottles

[Jones, Laura]

We have had continuing problems when pouring some stain reagents out of the 
gallon jugs in which they are sold.  We have all ruined shoes and scrubs from 
the splashing as the stain comes out of the bottle.  I thought I had seen some 
kind of no-splash spout adapter in the Marketlab catalog, but haven't been able 
to find anything.  Does anyone know if there is such an adapter and/or where we 
can get one?  Any suggestions or help appreciated.  Thanks!  Laura

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[Histonet] Lu Ann Aderson

[Laurie Colbert]

LuAnn,

 

If you are out there somewhere, would you mind giving me a call
regarding a CJD case?  If anyone knows how I can reach LuAnn, I would
appreciate her email address or phone number.

 

Laurie Colbert

Huntington Hospital

Pasadena, CA

(626) 397-8620

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RE: [Histonet] re; eosin

[Sherwood, Margaret]

I never noticed if ours shakes to rid the excess, but I do know the 95% alcohol
after eosin is pink but even when we stained manually, we had that as well.
What we do with the XL is rotate the 2nd 95% to that position and put fresh 95%
in the 2nd position.  However, we only do that daily, not after each run.  Even
manually, we made that switch daily, not after each run.   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Friday, January 15, 2010 1:04 PM
To: histonet
Subject: [Histonet] re; eosin

I have had some great suggestions from many of you, the problem is I am doing
literally all of them. My question is on the Leica XL isn't  the instrument
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it
is keeping a lot of carryover because it isn't vibrating. Also the alcohol after
eosin is pretty pink after only a few racks. Tell me what you
think...thanks, Cheri

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

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Re: [Histonet] re; eosin

[Rene J Buesa]

I think that you should talk with the person who sold you this refurbished 
instrument and present your problem. That will be more expeditious.
René J.

--- On Fri, 1/15/10, Cheri Miller  wrote:


From: Cheri Miller 
Subject: [Histonet] re; eosin
To: "histonet" 
Date: Friday, January 15, 2010, 1:04 PM


I have had some great suggestions from many of you, the problem is I am doing 
literally all of them. My question is on the Leica XL isn't  the instrument 
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why 
it is keeping a lot of carryover because it isn't vibrating. Also the alcohol 
after eosin is pretty pink after only a few racks. Tell me what you 
think...thanks, Cheri

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

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[Histonet] re; eosin

[Cheri Miller]

I have had some great suggestions from many of you, the problem is I am doing 
literally all of them. My question is on the Leica XL isn't  the instrument 
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why 
it is keeping a lot of carryover because it isn't vibrating. Also the alcohol 
after eosin is pretty pink after only a few racks. Tell me what you 
think...thanks, Cheri

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

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Re: [Histonet] Eosin evaporation.

[Rene J Buesa]

Before going home, cover the container
René J.

--- On Fri, 1/15/10, Cheri Miller  wrote:


From: Cheri Miller 
Subject: [Histonet] Eosin evaporation.
To: "histonet" , 
"histonet-boun...@lists..utsouthwestern.edu" 

Date: Friday, January 15, 2010, 10:05 AM


I recently purchased a leica refurbished autostainer, Before we hand stained 
our by hand. Now I notice the eosin is getting very low very fast, we have to 
replenish almost everyday. Before a bottle of Richard Allen Eosin would last 
the week or at least six days. Any idea's?

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148


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[Histonet] RE: Experience with Tissue Tek VIP6 processor

[Bell, Lynne]

We just installed a Tissue Tek VIP6 processor.  So far I love it!  Please feel 
free to email me with any questions.  I know that the Histonet is a great 
resource - they helped lead me to purchasing this unit.

Lynne

Lynne A. Bell, HT (ASCP)
Lead Histologist
Central Vermont Medical Center
130 Fisher Road
Barre, VT  05641
802-371-4923


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[Histonet] Mitochodrial IHC marker in FFPE skeletal muscle

[Sarka Lhotak]

Hi all,

I need to do a STAT3 IHC on mouse skeletal muscle. It should be showing
up in mitochondria. Before purchasing the antibody I am wondering if
the mitochodria are well enough preserved in FFPE tissue to show the
distinct mitochondrial pattern. Is there a reliable mitochondrial
"marker" (or a good mitochondrial protein antibody) that works on FFPE
tissue?

Thanks for your help,

Sarka Lhotak

McMaster university, Hamilton, Ontario  

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RE: [Histonet] Experience with Tissue Tek VIP6 processor

[Kathy M. Gorham]

I would like that information also please.   Kathy Gorham, H.T. 


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Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimmie
Rabe
Sent: Friday, January 15, 2010 8:05 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Experience with Tissue Tek VIP6 processor

Our lab is in the market for a new processor.  Does anyone have
experience with the Tissue Tek VIP 6?  And does anyone have experience
using it xylene-free?  Our histology supervisor would love to talk with
you.

Kim Rabe, M.D.
North Central Pathology, P.A.

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Re: [Histonet] Nuclei staining

[John Kiernan]

With the methods you list, you should easily be able to get selective nuclear 
staining with haemalum ("haematoxylin") or with Weigert's haematoxylin. The 
other traditional method, which is specific for DNA, is the Feulgen reaction. 
All these methods are in every book of staining techniques
 
John Kiernan.
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: "Tilston, Valerie" 
Date: Friday, January 15, 2010 6:08
Subject: [Histonet] Nuclei staining
To: "'histonet@lists.utsouthwestern.edu'" 

> Hi,
> 
> I'm trying to find a  stain for nuclei only (without a high 
> background stain) so that I am able to count the number of 
> nuclei present in paraffin fixed livers.  I appreciate that 
> I could do DAPI immunofluorescence or IHC etc but does anyone 
> know of any basic histology stains/protocols that give good 
> results? I've already tried haematoxyin, nuclear fast red, 
> carbol fuschin &  Weigerts A+B.
> 
> Many thanks,
> 
> Val
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RE: [Histonet] Eosin evaporation.

[Rathborne, Toni]

Are you keeping the lid to the stainer closed at all times, and are you 
covering each reagent container overnight? It should have come with a lid for 
each container.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Cheri
Miller
Sent: Friday, January 15, 2010 10:06 AM
To: histonet; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Eosin evaporation.


I recently purchased a leica refurbished autostainer, Before we hand stained 
our by hand. Now I notice the eosin is getting very low very fast, we have to 
replenish almost everyday. Before a bottle of Richard Allen Eosin would last 
the week or at least six days. Any idea's?

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148


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[Histonet] MGMT mutation analysis

[Rathborne, Toni]

We have a physician request for an MGMT mutation analysis on a paraffin block 
of a lung resection, previously diagnosed as malignant melanoma. I'm looking 
for a lab that performs this test. Reference labs feel free to reply.

Toni Rathborne
Pathology Supervisor
Somerset Medical Center
110 Rehill Ave.
Somerville,NJ 08876
908-595-2367



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Re: [Histonet] Eosin evaporation.

[Paula Pierce]

During the winter months the air is drier, especially with central heat. 

My tap water is sometimes as if it has been refrigerated too.

Paula





From: Sheila Haas 
To: Cheri Miller ; "Sherwood, Margaret" 
; "Breeden, Sara" ; "Mahoney, 
Janice A" ; histonet 
; 
"histonet-boun...@lists.utsouthwestern.edu" 

Sent: Fri, January 15, 2010 10:22:32 AM
Subject: Re: [Histonet] RE: Eosin evaporation.

Cheri,
How close on your stain line is your eosin in relation to the oven?
It's possible if it nearby, the oven is evaporating your eosin more
rapidly than expected. Just a thought.
 
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories
 





From: Cheri Miller 
To: "Sherwood, Margaret" ; "Breeden, Sara" 
; "Mahoney, Janice A" ; 
histonet ; 
"histonet-boun...@lists.utsouthwestern.edu" 

Sent: Fri, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.

We do this as well, that is why I am stumped.

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

-Original Message-
From: Sherwood, Margaret [mailto:msherw...@partners.org]
Sent: Friday, January 15, 2010 9:53 AM
To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.

I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also try to limit the sections to the bottom half or third of the sldie.



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Re: [Histonet] RE: Eosin evaporation.

[Sheila Haas]

Cheri,
How close on your stain line is your eosin in relation to the oven?
It's possible if it nearby, the oven is evaporating your eosin more
rapidly than expected. Just a thought.
 
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories
 





From: Cheri Miller 
To: "Sherwood, Margaret" ; "Breeden, Sara" 
; "Mahoney, Janice A" ; 
histonet ; 
"histonet-boun...@lists.utsouthwestern.edu" 

Sent: Fri, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.

We do this as well, that is why I am stumped.

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

-Original Message-
From: Sherwood, Margaret [mailto:msherw...@partners.org]
Sent: Friday, January 15, 2010 9:53 AM
To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.

I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also try to limit the sections to the bottom half or third of the sldie.



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RE: [Histonet] RE: Eosin evaporation.

[Cheri Miller]

We do this as well, that is why I am stumped.

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

-Original Message-
From: Sherwood, Margaret [mailto:msherw...@partners.org]
Sent: Friday, January 15, 2010 9:53 AM
To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.

I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also try to limit the sections to the bottom half or third of the sldie.



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[Histonet] Experience with Tissue Tek VIP6 processor

[Kimmie Rabe]

Our lab is in the market for a new processor.  Does anyone have experience with 
the Tissue Tek
VIP 6?  And does anyone have experience using it xylene-free?  Our histology 
supervisor would
love to talk with you.

Kim Rabe, M.D.
North Central Pathology, P.A.

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RE: [Histonet] RE: Eosin evaporation.

[Sherwood, Margaret]

I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also try to limit the sections to the bottom half or third of the sldie.

Peg 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, January 15, 2010 10:22 AM
To: Mahoney,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.

Eosin is alcohol-based, thus the evaporation. The containers on my
autostainer get covered as soon as the last run is finished and that
slows evaporation somewhat but I do replenish as needed. I like my eosin
level in the container a little lower so we don't have pink-stained
frosted ends, but that's just me!

Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM  87106
505-841-2576


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Re: [Histonet] Fabric Softener sheets

[Michael Smeltzer]

We placed a humidifier right next to our cryostat and kept movement by the
cryostat to a minimum to preserve the "shroud of humidity". Worked like a
charm when neither dryer sheets, grounding, a beaker of ethanol, or light
breathing into the chamber helped.

On Wed, Jan 13, 2010 at 4:34 PM,  wrote:

> Hello all,
>
>
> When using a dryer sheet in the cryostat to reduce static-
> has anyone experienced an increased amount of artifact with fluorescein
> stained slides???
> We will occasionally get a slide that has lots of fluorescent fibers all
> over the section!
> I vaguely remember being told that dryer sheets are not good for labs that
> cut cryostat sections for either  muscle histochemistries /or kidney
> sections for immunofluorescent staining...
>
> I think using a small container of alcohol placed into the cryostat will
> also help reduce static-
> These dry months kill us
>
> Thanks in advance
>
> Theresa
> Theresa R Dobersztyn HT (ASCP)
> Senior Technologist-Histology/Electron Microscopy Labs
> Dept. of Pathology and Laboratory Medicine
> Phone: 330-543-8279
> Fax: 330-543-3226
> tdobersz...@chmca.org
>
>
> Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best
> Workplace" award!
>
> *
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RE: [Histonet] RE: Eosin evaporation.

[Breeden, Sara]

Eosin is alcohol-based, thus the evaporation. The containers on my
autostainer get covered as soon as the last run is finished and that
slows evaporation somewhat but I do replenish as needed. I like my eosin
level in the container a little lower so we don't have pink-stained
frosted ends, but that's just me!

Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM  87106
505-841-2576


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[Histonet] RE: Eosin evaporation.

[Mahoney,Janice A]

Hi Cheryl,
Maybe the auto stainer does not drain the rack a well or it could just be a 
matter of the dipping and dunking that is causing more evaporation.
Jan
Omaha, NÉE

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Friday, January 15, 2010 9:06 AM
To: histonet; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Eosin evaporation.

I recently purchased a leica refurbished autostainer, Before we hand stained 
our by hand. Now I notice the eosin is getting very low very fast, we have to 
replenish almost everyday. Before a bottle of Richard Allen Eosin would last 
the week or at least six days. Any idea's?

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148


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[Histonet] Eosin evaporation.

[Cheri Miller]

I recently purchased a leica refurbished autostainer, Before we hand stained 
our by hand. Now I notice the eosin is getting very low very fast, we have to 
replenish almost everyday. Before a bottle of Richard Allen Eosin would last 
the week or at least six days. Any idea's?

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148


PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you 
are not the addressee intended / indicated or agent responsible for delivering 
it to the addressee, you are hereby notified that you are in possession of 
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Re: [Histonet] Unsuscribe

[Peter Carroll]

At least we've progressed from people asking to "unscribe"! Hahahaha...

Emily Sours wrote:

MY BRAIN JUST EXPLODED.
USE.
THE.
LINK.
AT.
THE.
BOTTOM.
OF.
HISTONET.
EMAILS.

Emily

...the thrill of being close to that hidden knowledge. That's the way I feel
when I read Nabokov. Encrypted within his words, encoded indecipherably,
ambiguously, is the equivalent of the secret of lightning. Something akin to
the secret code of higher human consciousness, the DNA, the genome of
genius.
-Ron Rosenbaum


On Thu, Jan 14, 2010 at 3:56 PM, Lorena Benedetti
wrote:

  

Unsuscribe

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Re: [Histonet] Unsubscribe

[Peter Carroll]

> Unsubscribe

Impossible... sorry, you're stuck on here FOREVER! ;)


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Re: [Histonet] requirements for certification

[Kim Merriam]

Hi Jen,

I am HT certified and have spent my entire career working in 
research/veterinary pathology.  I received my HT years ago when I was working 
for a preclinical contract lab, I had one of our board-certified veterinary 
pathologists sign the form for me (I work for a biotech company now and did the 
same thing when I took my QIHC a couple of years ago).  It looks like you have 
the proper education and experience, I think all you would need is for a 
board-certified pathologist (either DVM or MD) to sign the form that they are 
familiar with your work and you should be good to go.
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 





From: Jennifer Anderson 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Wed, January 13, 2010 4:50:53 PM
Subject: [Histonet] requirements for certification

Hello.

I'm thinking about getting my HTL certification.  I've been a bench scientist 
in both academia and biotech for 21 years, which has included pre-clinical 
animal research and subsequent histological applications.  I've set up an 
histology lab and have a lot of IHC but only basic staining (mostly H&E), as 
well as loads of animal tissue processing and some human tissues.  I have a 
liberal arts degree (an AB) with a biology major and a chemistry minor.  We 
have both a DVM and a few MD's on site, but for pre-clinical and clinical 
research consult.  I would appreciate any insight on getting certified through 
an online program, how many hours is required, and what kind of mentorship is 
necessary.

Thank you for your insights and time!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
jander...@halozyme.com


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[Histonet] Nuclei staining

[Tilston, Valerie]

Hi,

I'm trying to find a  stain for nuclei only (without a high background stain) 
so that I am able to count the number of nuclei present in paraffin fixed 
livers.  I appreciate that I could do DAPI immunofluorescence or IHC etc but 
does anyone know of any basic histology stains/protocols that give good 
results? I've already tried haematoxyin, nuclear fast red, carbol fuschin &  
Weigerts A+B.

Many thanks,

Val
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