[Histonet] Frozen Sections Slides per Day Question
Hi dear histology experts, I will greatly appreciate if somebody can let me know the estimated number of slides (with 3 sections per slide) per day on average a lab technician can cut (of frozen tissue). I am a lab technician ( not a histotech) and work alone in a highly complex testing specialzed low volume dermatology lab.My job description includes 30 % of immunohistochemistry related dutites. I cut skin frozen sections and do immunohistochemistry manually for several years . My routiene panel consists of 12 antibodies for T-cells surface markers. Ocassionally I add another panel of 8 antibodies for B-Cells. I am very slow in cutting sections and strugle a lot to get good sections. If I spend entire day ( 8 hours) just cutting 3-4 section on each slide with slow speed. What number of slides should be considered as efficent cutting? What number of blocks (12 slides for each block with 3-4 sections) should I finish in one day? Help me please if you can. Thanks. Soofia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Derm Lab
Raj, If you answer Eric's email I may help you too, because I set up a highly complex testing lab at dermatology starting from scratch with an empty room when they were starting the remodeling the room. The room may have already been equipped with ventilation, plumbing and electrical but they still had to put outlets and design the working are and bench surface. Soofia --- On Wed, 12/30/09, Eric Sulkosky wrote: From: Eric Sulkosky Subject: [Histonet] Derm Lab To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 30, 2009, 8:40 AM RAJ, What tips are you looking for? Are you starting from scratch with an empty room or has the room already been equipped with ventilation, plumbing and electrical? Eric ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] autofluorescence experiment
Hello, All, I am working on doing some IF stains with bone samples (lucky me!). I am having a difficult time sometimes to assess the antibody since the autofluorescence gets in the way. I am using undecalcified, FFPE sections (late embryo and neonate mouse bones). Without treatment, I see autofluorescence everywhere, but most frustrating is the red blood cells and the mineralized matrix. It took me a while to get samples that are properly fixed, section well, and stay on the slides, so I am not particularly jazzed about messing with the fixation protocol. Thus, I conducted an experiment today of several published and voodoo methods to reduce autofluorescence with samples that did NOT undergo the IF protocol : no treatment photobleaching, fluorescent light box, up to 2 weeks photobleaching, UV crosslinking light, 2 inches from source, 24h 10mM copper sulfate in 50mM ammonium acetate, pH 5.0 0.25% (v/v) NH3 in 70% ethanol (in water) 0.25% (v/v) NH4OH in 70% ethanol (in water) (since it was unclear from the published reference what the source of ammonia was, and I have made this mistake before on some other thing) 0.3% (w/v) Sudan Black in 70% ethanol (in water) I found that the most effective treatment in my hands is Sudan Black for cell-based autofluorescence, but it did not seem to impact the autofluorescence from the mineralized matrix at all, while copper sulfate had significant impact on the autofluorescence in mineralized bone, but did not quench cell-based autofluorescence as well as the Sudan Black (it had an even but incomplete impact over the entire section). I have tried Sudan Black on my slides before, and have found that it did not seem to interfere significantly with the antibody signal. I modified the Sudan Black protocol to eliminate the "goopies" and "chunkies" resulting on my slides from previous attempts, and am happy with the results- a light, even stain. My question to all you chemistry folks: Is there some reason why copper sulfate treatment followed by washing and subsequent Sudan Black treatment (and more washing) cannot or should not be used? I tried them together on my unstained slides, they look pretty darn fabulous. Just striving for clean data and beautiful pictures. Thanks again for all your help, Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: FFPE Microwave processed biopsies
-Original Message- From: Chad Miller [mailto:cmil...@labmd.org] Sent: Wednesday, February 03, 2010 4:46 PM To: Delaney, Sondra L Cc: Chad Miller Subject: FFPE Microwave processed biopsies Microtomy Issue: Our lab has recently switched from using GTF (Glycol Tissue Fixative) as a primary fixative to10%NBF for prostate biopsies. I am experiencing issues with wrinkles, to varying degrees, along the length of the biopsies especially around the lumens of glandular structures. Sections are taken at 3 micrometers with zero visible compression/wrinkling/washboarding etc... of the tissue. When the ribbon is placed on the water bath (47-50 C) the tissue seems to contract and wrinkling appears although, only in the tissuesimilar to the coils of a compressed slinky. I have adjusted the water bath temp. increasingly higher until my wax can no longer stand the temp and dissipates around the tissue. This has not cured the wrinkling problem but has shown some improvement. Prostate biopsies that have been fixed in GTF and processed identically to the formalin fixed biopsies are simply perfect in every way. Specimens are embedded using Polyfin (TBS, M.P. 55C) and kept cool for sectioning on ice trays. Sections are collected on charged Histobond slides. Wrinkles appear whether processing solutions are brand new or have been used for previous runs. If anyone has any suggestions ideas or experiences I would sincerely appreciate them. I have included processing times below. Specimen Type: Prostate Needle Core Biopsy Processor: Milestone Histos 5 (Microwave) Specimen Fixative: 10%NBF Specimen Prep: Aligned in linear fashion and sandwiched between sponges to maintain orientation. Max Block Volume: 110 blocks/run Offline Prep: 1. 70% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) 2. 95% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) 3. 100% ETOH Rinse (Manual @ 5minutes)--(Changed every 450 blks) Histos 5 Processor: (0-55blk processing protocol) 1. 100% ETOH (15 minutes @ 65 C)--(Changed every 220 blks) 2. Isopropyl (16 minutes @ 68 C)--(Changed every 220 blks) 3. Vacuum Step (2 minutes ambient) 4. Paraffin (Paraplast M.P.56 C) (23 minutes @ 68 C with Vacuum)-(Changed every 450 blks) This e-mail may contain identifiable health information that is subject to protection under state and federal law. This information is intended to be for the use of the individual named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited and may be punishable by law. If you have received this electronic transmission in error, please notify us immediately by electronic mail (reply). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slide drying
Place the slides in every other slot of basket and dry for 15-20 min at 69 celcius, let cool in front of fan for 1-2 min. You are able to combine baskets at this step. Kyle HT BS Nacogdoches Memorial Our lab just received a slide drying oven, and we are trying to figure out what's a good temperature for the slides to be heated and for how long. We are mainly doing H&E slides. The way we are currently drying them is under a small fan, then heat them up, and back to the fan. Then finally heating them up and off to the stainer. Just putting them in the slide drying oven for 10 min at 80C melts the paraffin, but leaves some water on the bottom of some sections. Any suggestions to get the slides dry asap ? Also is the oven much help to your labs? Thanks ! Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TRAP assay - acid phosphatase
We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling with the protocol it works pretty good Liz From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sherwood, Margaret Sent: Wed 2/3/2010 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TRAP assay - acid phosphatase Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TRAP assay - acid phosphatase
Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on mouse tibia for IHC. If so, 1) can you do it on paraffin and/or frozen sections? 2) if you do it on frozens, do you decalcify first? 3) what type of blade do you use for sectioning on the cryostat? 3) what procedure do you follow after sectioning: do you fix slides (i.e. acetone, etc.) Thank you. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Drying ovens, eosin fading, microtome PM, eyeballs
Our routine sections, picked up on regular slides with no adhesive in the water bath, dry at 65C for 15 minutes in the oven on our Leica stainer XL, we never lose sections. Sections for IHC and specials go on Plus slides and dry in an oven also at 65C for at least 1 hour. Then they get the same Leica XL oven drying cycle before deceration. Fading eosin usually means incomplete dehydration, trace water in the xylene etc. Belair does all our PM's, microtomes, cryostats, they've kept our 30 year old VIP running despite the fact that Sakura no longer supports the oldest models (like ours). Eyeballs should not be opened until they have been fixed for at least two days intact in 10% buffered formalin. Then, using a brand new blade, slice off the two callottes, proceeding from cornea posteriorly to the optic nerve, from a central section which includes the optic nerve and the pupil. Some retinal separation is inevitable, this technique will minimize it. Jeffrey Silverman, HT HTL QIHC Pathologists' Assistant Southside Hospital NSLIJHS Bay Shore, New York USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microarray info thanks!
Thank you everyone for all the information about microarray equipment. As usual on the Histonet, everyone was quick with the information, generous with their time, and informative. Michael Titford USA Pathology Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: P63 RUO
It doesn't matter what machine you run them on or if you do them by hand. You cannot bill for anything that is RUO.ASR and IVD's muct be properly validated before you bill for them as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine [sharon.davis-dev...@carle.com] Sent: Wednesday, February 03, 2010 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 RUO Another question for the group of experts. Is it true that you cannot bill for antibodies that are classified as a RUO for Ventana? Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P63 RUO
Another question for the group of experts. Is it true that you cannot bill for antibodies that are classified as a RUO for Ventana? Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin leaching out of sections
Where do you get the beads from? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Joyce Cline Sent: Wednesday, February 03, 2010 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eosin leaching out of sections Humidity has played a large part in our slides leaching Eosin. We have a dehumidifier in our staining room and we also use H2Blue Beads in our Xylene substitute. The beads absorb water from the substitute and help prevent leaching of the stain. The beads are ordered from Amercian Mastertech. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin leaching out of sections
Humidity has played a large part in our slides leaching Eosin. We have a dehumidifier in our staining room and we also use H2Blue Beads in our Xylene substitute. The beads absorb water from the substitute and help prevent leaching of the stain. The beads are ordered from Amercian Mastertech. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: GI Biopsies
What kind of stainer do you have? Jan, Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anderson, David W Sent: Wednesday, February 03, 2010 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies We are having an issue with our GI Biopsy H&E Stains. On our GI Biopsies, we currently cut eight slides, staining levels 2, 5 and 8 with H&E. The pathologist are noticing that the level two slide looks great but then levels 5 and 8 will have what they call "Blue Blobs" on the tissue sections. They tend to look like formalin salt crystals. Does anyone else experience this? Do any of you have a resolution to this problem? Thank you so much for your help with this matter. David Anderson,HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] P63
We use an IVD p63 (cat# CM163) from BioCare Medical. Contact me if you'd like further info. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 03, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] P63
>From Cell Marque -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 03, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P63
Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GI Biopsies
We are having an issue with our GI Biopsy H&E Stains. On our GI Biopsies, we currently cut eight slides, staining levels 2, 5 and 8 with H&E. The pathologist are noticing that the level two slide looks great but then levels 5 and 8 will have what they call "Blue Blobs" on the tissue sections. They tend to look like formalin salt crystals. Does anyone else experience this? Do any of you have a resolution to this problem? Thank you so much for your help with this matter. David Anderson,HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] thermal printer microscope labels
Just remember that some thermal labels will fade over time. Be sure your label will still be legible in 10 years. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, February 03, 2010 11:55 AM To: 'Paul Lambert'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermal printer microscope labels The key thing to note with slide labels is that it's not JUST the label. It's the combination of the label, ribbon, and printer. As an LIS vendor, I've heard very, very good things about General Data, and I do know that GD will sell you the label, ribbon, printer and even the bar code scanner. If you have any further questions, feel free to email me. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paul Lambert Sent: Wednesday, February 03, 2010 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermal printer microscope labels Does anyone have experience with printed plastic labels for microscope slides that are claimed to be resistant to various solvents and chemical used in histolology? If so what product do you use? One company (General Data) apparently has a special version of their label that is compatible with heat-based antigen unmasking steps... Any experience there as well? Appreciate the feedback Thanks Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 54706 USA tel: 608-262-8533 fax: 608-262-2824 email: plamb...@wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] thermal printer microscope labels
The key thing to note with slide labels is that it's not JUST the label. It's the combination of the label, ribbon, and printer. As an LIS vendor, I've heard very, very good things about General Data, and I do know that GD will sell you the label, ribbon, printer and even the bar code scanner. If you have any further questions, feel free to email me. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paul Lambert Sent: Wednesday, February 03, 2010 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermal printer microscope labels Does anyone have experience with printed plastic labels for microscope slides that are claimed to be resistant to various solvents and chemical used in histolology? If so what product do you use? One company (General Data) apparently has a special version of their label that is compatible with heat-based antigen unmasking steps... Any experience there as well? Appreciate the feedback Thanks Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 54706 USA tel: 608-262-8533 fax: 608-262-2824 email: plamb...@wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] labels
We use the labels from General Data through Dako and are happy with the printing and the resistance to solutions. They adhere well and go through all types of staining from H&E to IHC and are placed in the slide dryer. We have treid both the flapless labels and those with the protective "flaps". Dorotohy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome PM
You can contact Belair in NJ at 800-783-9424 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of S R Sent: Wednesday, February 03, 2010 10:54 AM To: histo net Subject: [Histonet] Microtome PM Hello Everyone. Does anyone know if there is anyone in the northeast that does microtome pm's? The microtome is a Microm Hm 315. I do not think it has ever had a pm and it is starting to act up. Thanks in Advanced. Sammy _ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Eosin leaching out of sections
Scott, Did you mount from water or a clearing agent? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tape/Film Coverslips vs. Glass
Glass is generally better for photomicrotomy also. Pam Marcum UAMS - Original Message - From: "Cynthia Pyse" To: "Cathy Crumpton" , histonet@lists.utsouthwestern.edu Sent: Wednesday, February 3, 2010 8:21:15 AM GMT -06:00 US/Canada Central Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome PM
Sammy, Try Michael Dietrich at Southeast Pathology Instrument Services, Inc at 843-588-2559. I don't know how far north he is willing to go but he is the best in the business! You can tell him Wanda said so!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of S R Sent: Wednesday, February 03, 2010 10:54 AM To: histo net Subject: [Histonet] Microtome PM Hello Everyone. Does anyone know if there is anyone in the northeast that does microtome pm's? The microtome is a Microm Hm 315. I do not think it has ever had a pm and it is starting to act up. Thanks in Advanced. Sammy _ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome PM
Hello Everyone. Does anyone know if there is anyone in the northeast that does microtome pm's? The microtome is a Microm Hm 315. I do not think it has ever had a pm and it is starting to act up. Thanks in Advanced. Sammy _ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thermal printer microscope labels
Does anyone have experience with printed plastic labels for microscope slides that are claimed to be resistant to various solvents and chemical used in histolology? If so what product do you use? One company (General Data) apparently has a special version of their label that is compatible with heat-based antigen unmasking steps... Any experience there as well? Appreciate the feedback Thanks Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 54706 USA tel: 608-262-8533 fax: 608-262-2824 email: plamb...@wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tape/Film Coverslips vs. Glass
Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin leaching out of sections
Your xylene and/or alcohols may need changing. Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Scott Hendricksen Sent: Wed 2/3/2010 9:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections > Hi again, > > Does anyone have an issue with eosin leaching out of sections a few days > after they have been coverslipped on an H&E stained slide? > > Thanks, > > Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin leaching out of sections
> Hi again, > > Does anyone have an issue with eosin leaching out of sections a few days > after they have been coverslipped on an H&E stained slide? > > Thanks, > > Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Veronica Cedeño te ha dejado un mensaj e...
Veronica Cedeño te ha dejado un mensaje... El mensaje y la persona que lo envió solo te será mostrado a ti y borrarlo en cualquier momento. Puedes responder a través del sistema de intercambio de mensajes. Para descubrir quién te escribió, sigue el siguiente link: http://us1.badoo.com/01107496637/in/9.VDNlA6QFg/?lang_id=7 Más gente que también te está esperando: FRANKLIN XAVIER (Portoviejo, Ecuador) Kritos beauty (Portoviejo, Ecuador) Xavier (Portoviejo, Ecuador) http://us1.badoo.com/01107496637/in/9.VDNlA6QFg/?lang_id=7 Si al pulsar el enlace de este mensaje no funciona, copia y pégalo en la barra de tu navegador. Este email es parte del procedimiento del sistema para el envío de mensajes enviados por Veronica Cedeño. Si has recibido este mensaje por error, ignora este email. Tras un corto periodo de tiempo, será eliminado del sistema. ¡Divértete! El equipo de Badoo Has recibido este email porque un usuario de Badoo te ha dejado un mensaje en Badoo. Este mensaje es automático. Las respuestas a este mensaje no estan controladas y no serán contestadas. Si no quieres recibir más mensajes de Badoo, háznoslo saber: http://us1.badoo.com/impersonation.phtml?lang_id=7&mail_code=21&email=histonet%40lists.utsouthwestern.edu&secret=&invite_id=411714&user_id=1107496637 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin leaching out of sections
Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an H&E stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC protocol for vimentin in animal tissue
Hello Lynn I work in a veterinary institution and use a Benchmark. Our vimentin works very well. Our protocol is stdCC1,16m. Good luck! Kathy Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microwave processor
I cannot help you with your specific enquiry, re the microwave processor, but gently suggest you learn from this experience and keep a comprehensive record of methods/techniques used in your laboratory, good luck anyway -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of RP Sent: 03 February 2010 00:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processor Hello All, I have a dilemma and was hoping you guys could help me. I just took on a part time job in a small GI lab and the previous tech working there left without no one knowing how to use the microwave processor and there's no procedure. I was wondering if anyone has used the Lab pulse Microwave Tissue Processor H 2850 by EBSciences and if there is a procedure for small BX's. I see nothing but magnets on the side and there is no letters or numbers indicating where the magnets are placed. Also I've never used one before. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alcohol fixation and immuno
You did not specify which enzyme you were using, but I am assuming proteinase K. If an hour's retrieval is too much, and no retrieval is too little, I would suggest running some (alcohol fixed) controls at 15, 30, and 45 minutes in the 95C, or 1,2,3, and 4 minutes in the enzyme. Evaluate the slides with a pathologist for staining vs. section adhesion. Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
